Aim: To explore the effect of long-term exercise of shadowboxing on the psychological factors in middle and aged people. Methods: Middle and aged people engaged in shadowboxing in Jinan were randomly selected as the subjects. Data about psychological stress, interpersonal relationship, mental age were obtained by the survey of questionnaire. Results: The state of mental health of middle and aged people who were accustomed to doing exercise in Jinan was better, there were 6% of them whose actual age <49, while 100% of them whose mental age <49. There was no difference in mental age between people engaged in shadowboxing and other people (χ2 = 5.49, P > 0.05). There were significant differences in the scores of psychological stress and interpersonal relationship between different activity groups (χ 2 = 18.00-41.82; P < 0.01). Conclusion: Long-term shadowboxing exercises can reduce the psychological stress of middle and aged people, influence their mental stress significantly, and promote the better development of interpersonal relationship.