Microscopic, Physical and Mechanical properties of Mulberry, Eri Muga, Tasar and peduncle Tasar silk fibre have been studied according to international standard testing methods. Results have been compared as per the requirement of industry. The results of the investigation thoroughly discussed It is revealed that Tasar, Eri and Muga silk has elongated triangular cross section, Mulberry silk has rounded triangular cross section. Mulberry silk is finest and strongest fiber, Tasar silk is coarser and weakest fiber, Muga silk fibre has highest elongation percentage than other silk with better strength than Tasar but lesser than Eri, mulberry silk fibre.