Effects of Eutypa lata, the fungal agent of eutypiosis, on polyamine (putrescine (Put), spermidine (Spd), spermine (Spm), diaminopropane (Dap)) and tyramine (Tyr) contents in grapevine leaves were studied to determine the relationship between these components and disease development. The external symptoms of eutypiosis are small and necrotic leaves, which could be observed on only one arm or on ... [Show full abstract] two arms of the vine stock. This study was carried out on healthy leaves (S, borne by healthy vine stock), apparently healthy leaves (Aps, borne by one arm without symptoms, whereas the other arm was diseased), and moderately (M. mal.) and strongly (F. mal.) diseased leaves. We analysed free polyamines (free PAs, chlorhydric acid soluble fraction), conjugated polyamines (PAs-fpm, PAs covalently linked to molecules with low molecular mass like hydroxycinnamic acids), and bound polyamines (PAs-mm, covalently linked to macromolecules) in Vitis vinifera L. 'Cabernet sauvignon', a variety sensitive to eutypiosis. In diseased leaves, we noted a decrease of free PAs, PAs-fpm, free Tyr, and Tyr-fpm levels. By contrast, the Dap-fpm level was increased by the disease. Eutypiosis also induced an increase of Spm-mm and Dap-mm levels, whereas the Put-mm and Spd-mm levels were decreased. Free Put, free Spd, PAs-fpm, free Tyr, and Tyr-fpm levels were higher in apparently healthy leaves than in healthy leaves. The PAs-mm levels were slightly affected by eutypiosis in apparently healthy leaves. The specific role of each type of polyamines and tyramine is discussed with regard to the host-parasite relationship.Key words: polyamines, leaf, Eutypa lata, Vitis vinifera.