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S5 Molecular classification and annotation of selected Phosphomotifs showing strong interaction with endogenous GRB2 from breast normal and cancer cells
Reporter Name MCF10A MCF7 T47D MDA-MB-231
Receptors Signal Intensity
Rceptor Tyrosine Kinases
CSF1R_923 2132.82 3326.84 14204.79 3911.82 yTNLPS
CSF3R_787 23.64 39784.34 8264.28 1344.52 yENLWF
EGFR_113 159.36 13348.42 6649.81 1105.25 yENSYA
FLT1_1213 1243.70 50329.84 42773.63 5265.04 yVNAFK
FLT3_768 3346.64 60294.52 47482.62 5622.40 yENQKR
FLT3_955 692.92 19028.31 4620.21 2764.02 yQNVDG
FLT3_969 20582.89 1740.46 15343.27 6024.30 yQNRRP
MET_1356 559.03 3902.83 5867.53 2945.71 yVNVKC
PDGFRB_716 1170.99 11085.63 1339.97 1912.86 ySNALP
TIE1_1117 104.00 6881.89 3256.48 572.03 yVNMSL
Other receptors
EPOR_489 90.03 20435.75 21415.85 1251.54 yENSLI
PILRA_246 1347.84 6395.42 13985.79 4050.55 yENIRN
PTPRA_295 1.00 3289.62 1.00 657.20 yINASF
PTPRA_798 1286.91 11705.29 25480.48 4155.81 yANFK
PTPRC_1015 1.00 3215.14 1.00 681.71 yINASF
LIRB4_412 4613.77 4035.71 4372.06 6801.10 yARLHS
IL1RL1_99 426.15 2332.21 15882.67 1472.26 yANVTI
Signaling adaptors
SOS1_974 1133.47 56630.19 35405.88 6737.76 yQNQPY
FRS2_196 1299.14 18237.29 17453.82 2861.65 yVNTTG
IRS1_46 559.26 8414.52 6619.40 433.41 yYENEK
IRS1_47 962.60 53236.66 18388.82 1093.66 yENEKK
IRS1_612 1678.35 1042.01 5174.43 1006.55 yMPMSPG
IRS1_632 3259.79 944.00 6908.81 2977.46 yMPMSPK
Reporter
Sequence (N' to
C')
IRS1_896 40.37 4777.99 3742.82 1369.10 yVNIEF
IRS1_941 15.76 5455.87 1483.42 345.72 yMNMDL
IRS4_921 1.00 7686.77 3264.65 513.84 yVNMDF
SHC1_349 302.29 4908.84 488.92 306.07 yYNDFP
SHC1_427 631.05 6357.72 15424.05 3265.95 yVNIQN
PTPN11_279 3687.78 3170.98 13701.15 3070.10 yKNILP
PTPN11_546 94.84 22205.14 2627.97 1387.73 yTNIKY
PTPN11_584 28.70 28289.57 13791.17 1636.27 yENVGL
PTPN18_303 696.24 5771.67 3255.49 2229.09 yQNIKE
LAT_200 2429.98 25106.13 16252.66 3681.30 yVNVPE
LAT_220 972.14 36557.34 11143.63 2162.05 yVNVSQ
LAT_255 48.83 5838.26 9974.29 1528.02 yENLQE
LAX1_268 1409.30 19194.37 10462.40 2382.91 yVNMTG
LAX1_294 4367.68 22474.61 18570.96 4866.88 yENVPA
LAX1_373 2000.16 62569.89 42557.02 3559.71 yENVLT
NTAL_136 3019.42 21187.56 33979.91 9194.80 yQNFSK
NTAL_233 367.30 7261.03 1217.39 1604.09 yVNGEV
NTAL_95 60.69 49842.24 22332.39 2257.07 yENVLI
SIT1_188 4148.71 5476.31 11818.29 2852.00 yANSQP
SHIP1_556 1472.04 10661.11 27023.16 3236.18 yMNILR
Cyotplasmic TKs
FAK1_925 1439.62 59992.23 41303.45 3586.70 yENVTG
PAK1_464 1770.16 4104.25 2550.99 1592.94 yLNENP
Cytoskeletal Signaling
SPTA2_2430 23107.54 56317.28 50857.88 13934.16 yQNLTR
DYST_959 4886.17 56188.23 18325.11 8996.48 yQNVLT
MYPT1_67 104.58 4697.01 4038.31 1051.18 yANVDG
Ligand
TNFL6_258 487.91 17619.25 6887.68 2638.64 yVNVSE
Cell adhesion molecule
SIG10_597 5033.45 7245.86 6636.45 3604.85 yINVVP
RNA Binding Protein
LUC7L2_173 3630.02 3720.58 658.47 2207.75 yRNSMP
ETV6_314 426.82 4506.53 8044.14 1316.54 yMNHIM
NFAM1_220 1905.54 1266.47 16788.02 3604.45 yTALQR
Nuclear Transport Proteins
RANBP17_668 8234.32 1322.92 6604.43 3986.83 yTALTR
XPO7_669 933.94 158.69 9193.54 2399.73 yTALGR
Enzymes
GSTP1_199 2594.76 25267.94 45756.98 7675.11 yVNLPI
HEXB_277 1765.58 134.40 7013.36 1160.75 yARLRG
S5 Molecular classification and annotation of selected Phosphomotifs showing strong interaction with endogenous GRB2 from breast normal and cancer cells
Uniprot ID Reported PPBDs Name
P07333 GRB2
Macrophage colony-stimulating factor 1 receptor precursor
Q99062 SYK,SH3BP2
Granulocyte colony-stimulating factor receptor precursor
P00533 GRB2
Epidermal growth factor receptor precursor
P17948 PTPN11,GRB2,NCK1,PIK3R1(p85)
VEGFR1, Vascular endothelial growth factor receptor 1
P36888 GRB2 FL cytokine receptor
P36888 GRB2 FL cytokine receptor
P36888 SH3BP2 FL cytokine receptor
P08581 INPP5D,INPPL1,PTPN11,PLCG1,GRB2,PIK3R1 Hepatocyte growth factor receptor
P09619 GRB2,GRB7
Beta-type platelet-derived growth factor receptor
P35590 PIK3R1 Tyrosine-protein kinase receptor Tie-1
P19235 LYN,SH3BP2, Erythropoietin receptor
Q9UKJ1 PTPN11, PTPN6
Paired immunoglobulin-like type 2 receptor alpha
P18433 GRB2
Receptor-type tyrosine-protein phosphatase alpha
P18433 SRC,GRB2
Receptor-type tyrosine-protein phosphatase alpha
P08575 GRB2
Receptor-type tyrosine-protein phosphatase C precursor
Q8NHJ6 PTPN6
Leukocyte immunoglobulin-like receptor subfamily B member 4
Q01638 GRB2,MATK Interleukin-1 receptor-like 1
Q07889 GRB2 Son of sevenless homolog 1
Q8WU20 GRB2
Fibroblast growth factor receptor substrate 2
P35568 IRS1 Insulin receptor substrate protein
P35568 GRB2,SH3BP2 Insulin receptor substrate protein
P35568 IRS1 Insulin receptor substrate protein
P35568 IRS1 Insulin receptor substrate protein
P35568 IRS1\INSR Insulin receptor substrate protein
P35568 GRB2,PIK3R1 Insulin receptor substrate protein
O14654 GRB2,PIK3R1, SHC1, PLCG Insulin receptor substrate 4
P29353 GRB2,GRAP2,INPP5D SHC-transforming protein 1
P29353 GRB2 SHC-transforming protein 1
Q06124 GRB2
Tyrosine-protein phosphatase non-receptor type 11
Q06124 PTPN11,GRB2,FYN,SOCS3
Tyrosine-protein phosphatase non-receptor type 11
Q06124 PTPN11,GRB2,FYN
Tyrosine-protein phosphatase non-receptor type 11
Q99952 TEC
Tyrosine-protein phosphatase non-receptor type 18
O43561
GRAP2,GRAP,VAV1,PLCG1,GRB2,ZAP70,PIK3R1
Linker for activation of T cell
O43561 VAV1,GRB2,PLCG1,GRAP2,PIK3R1 Linker for activation of T cell
O43561 GRAP,VAV1,PLCG1,GRB2,ZAP70 Linker for activation of T cell
Q8IWV1 GRB2,PIK3R1 Lymphocyte transmembrane adapter 1
Q8IWV1 GRB2 Lymphocyte transmembrane adapter 1
Q8IWV1 GRB2 Lymphocyte transmembrane adapter 1
Q9GZY6 GRB2
Linker for activation of T-cells family member 2
Q9GZY6 GRB2
Linker for activation of T-cells family member 2
Q9GZY6 GRB2
Linker for activation of T-cells family member 2
Q9Y3P8 GRB2
Signaling threshold-regulating transmembrane adapter 1
Q92835 GRB2,INPP5D
Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1
Q05397 SRC,STAT3,SHC1,GRB2,FES,SH3BP2 Focal adhesion kinase 1
Q13153 GRB2 Serine/threonine-protein kinase PAK 1
Q13813 GRB2 Spectrin alpha chain, brain
Q03001 GRB2 Dystonin
O14974 GRB2
Myosin phosphatase-targeting subunit 1/Protein phosphatase 1 regulatory subunit 12A
P48023 GRB2
Tumor necrosis factor ligand superfamily member 6
Q96LC7 PTPN11,PTPN6 Sialic acid-binding Ig-like lectin 10
Q9Y383 GRB2
Putative RNA-binding protein Luc7-like 2
P41212 GRB2 Transcription factor ETV6
Q8NET5 SYK,ZAP70 NFAT activation molecule 1
Q9H2T7 PTPN6 Ran-binding protein 17
Q9UIA9 PTPN11,PTPN6 Exportin-7
P09211 GRB2 Glutathione S-transferase P
P07686 PTPN11,PTPN6 Beta-hexosaminidase subunit beta
S5 Molecular classification and annotation of selected Phosphomotifs showing strong interaction with endogenous GRB2 from breast normal and cancer cells
Signaling Pathways Reference
Oncogenic RTK YES autocatalysis Mancini A, et al. (1997)
cytokine signaling YES not known
Hunter MG, et al. (2004), de Koning JP, et al. (1996)
Oncogenic RTK NO
VEGFR signaling pathway YES autocatalysis Ito N et al (1998, 2001)
VEGFR signaling pathway YES Masson K, et al. (2009)
VEGFR signaling pathway YES Masson K, et al. (2009)
VEGFR signaling pathway YES Masson K, et al. (2009)
hepatocyte growth factor receptor signaling pathway
YES Fixman ED, et al.; Giordano S, et al. 1997
GF signaling, cell ligration YES Arvidsson AK, et al. (1994)
protein tyrosine-kinase transmembrane receptor-negative regulation of angiogenesis
YES Kontos CD et al (2002)
cytokine receptor family, JAK-STAT signaling
YES JAK2, LYN Sulahian R, Cleaver O, Huang LJ (2009)
Immune signaling. receptor -negative regulation of immune system
NO not known
Receptor-type tyrosine-protein phosphatase alpha
regulation of integrin signaling, cell adhesion and proliferation.
YES
Heibeck T.H., et al (2009). Chen M, et al (2006)
T-cell activation through the antigen receptor.
NO
Leukocyte immunoglobulin-like receptor subfamily B member 4
YES
Pighi C, et al. (2011), Rikova K, et al. (2007)
NF kappa B signaling, cytokine signaling; NO not known
Ras signaling NO not known
YES Hadari YR et al (1998)
Insulin signaling/ IGF1R YES Rush J. et al (2005), Rikova K.et al (2007)
Insulin signaling/ IGF1R NO
Insulin signaling/ IGF1R YES Esposito DL, et al (2001)
Insulin signaling/ IGF1R YES
Esposito DL, et al (2001), Heibeck, TH et al (2009)
Phosphorylation
reported
Probable Kinase
(s) that may
phosphorylate
Insulin GFR, FGFR, NGFR, GPCR signaling
EGFR mediated cytokine signaling through TNF and FGFR signaling?
YES
Sun XJ, et al. (1993), Myers MG, et al. (1994), Valverde AM, et al. (2001)
Insulin signaling/ IGF1R YES
Rikova K, et al. (2007) Hers I, et al. (2002)
SOCS binding, JAK-STAT signaling YES Hinsby AM. et al (2004)
Signaling adapter that couples activated growth factor receptors to signaling pathway.
YES
Patrussi L, et al. (2005); van der Geer P et al (1996)
Signaling adapter that couples activated growth factor receptors to signaling pathway.
YES
Ursini-Siegel J, et al. (2008); Patrussi L, et al. (2005)
participate in the signal transduction from the cell surface to the nucleus
YES Mitra S, et al (2008)
participate in the signal transduction from the cell surface to the nucleus
YES PDGFR
Bennett AM, et al. (1994), Herrera Abreu MT, et al. (2006)
participate in the signal transduction from the cell surface to the nucleus
YES PDGFR Araki T et al (2003)
GF signaling, YES TEC kinase Aoki N, et al. (2004)
T cell signaling YES ZAP70
Zhang W, et al. (1998, 2000), Malbec O, et al. (2004)
T cell signaling YES Malbec O, et al. (2004)
T cell signaling YES
Malbec O, et al. (2004), Mayya V.et al. (2009)
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor) mediated signaling
NO Syk, Lck or ZAP70 upon TCR or BCR activation
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor) mediated signaling
NO Syk, Lck or ZAP70 upon TCR or BCR activation
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor) mediated signaling
YES
Syk, Lck or ZAP70 upon TCR or BCR activation
Mayya V.et al. (2009)
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells, may be in B cell signaling
YES Iwaki S, et al. (2008)
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells, may be in B cell signaling
YES Iwaki S, et al. (2008)
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells, may be in B cell signaling
YES Iwaki S, et al. (2008)
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells
YES
Mayya V, et al. (2009) Rush J.,et al (2005).
Negative regulation of B cell signaling, Cytokine signaling
YES CST curated data
Ty Kinase, motility, oncogenic transformation
YES
Mitra SK, et al. (2006) , Kaneda T, et al. (2008)
S\T kinase, motility, polarity NO not known
Interacts with calmodulin through calcium signaling
YES
Pighi C, et al. (2011), Rikova K, et al. (2007)
Cytoskeletal signaling NO
Cytoskeletal signaling NO not known none
T cell signaling, T cell mediated apoptosis NO
YES Whitney G, et al. (2001)
not known NO
PDGF, Immune signaling YES Million, RP et al 2004
calcineurin/NFAT-signaling pathway , cytokine signaling,B cell signaling
NO only predicted ZAP70 and SYK
Yang J.et.al (2003). Ohtsuka M et al. (2004)
nuclear import receptor signaling NO
Mediates the nuclear export of proteins (cargos) with broad substrate specificity
NO
EGFR siganling YES not known Okamura T, et al. (2009)
CNS pathways? NO not known
Immune signaling, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal
transduction through dephosphorylation of signaling molecules