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Figure S2

January 2010

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Tg(hsp70l:unplugged SV1-myc); unpluggedbr307/br307 embryos were heat-shocked starting at the indicated times points, and examined at 27 hpf for motor axon pathfinding. Results were summarized from one experiment, analyzed as in Figure 3A, and represented as mean±SEM. 20 hemisegments were scored in each embryo (n = 180−400, average = 328, hemisegments per time point). (0.14 MB TIF)

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Jing L, Gordon LR, Shtibin E, Granato M. Temporal and spatial requirements of unplugged/MuSK function during zebrafish neuromuscular development. PLoS One 5:e8843

Article

January 2010

Lili Jing · Laura R Gordon · Elena Shtibin · Michael Granato

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Figure S4

January 2011

Motoneuron Survival. (A) ChAT staining in whole-mount paraffin section in the cervical region of a wild type E18 animal. Scale bar = 200 µm. (B) Comparison of total motoneuron numbers in wild type (white) and γ/ε-fc (gray) embryos at age E16 and E18. n = 10 spinal cord sections, 3 embryos per point. Error bars indicate SEM. (TIF)

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Figure S11

October 2011

Effects of low amounts of smc3-MO on gene expression. (A) Morphology of living embryos at 26 hpf. Lateral views with anterior to the left. (B) Q-PCR analyses of gene expression in uninjected control (black), nipbla/b-morphants (blue), and low-smc3 (0.75 ng)-morphants (red) at 25 hpf by Q-PCR (n = 3, mean ± S.E.M.; * p<0.05 and # p>0.1 by paired t test). Scale bar: 100 µm. (EPS)

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S7 Fig

November 2018

Quantification of the length of tenocyte processes. col1a2Kaede embryos at 5 dpf were imaged in transverse views and the length of tenocyte processes was measured for individual tenocytes. A representative image is shown in Fig 4D. Data is plotted with mean ± SEM indicated. n = 28 tenocytes from 15 embryos. (TIF)

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Supplementary Figure 1

October 2016

GLI3 binding to GLI targets is confirmed by ChIP. ChIP-qPCR analyses of GLI3 precipitated with GLI binding regions of ALX4 (A), BMPR1A (B), OSR2 (C), WIF1 (D), and PTCH1 (E) in facial prominences of control embryo. Error bars are based on the standard error of the means (S.E.M.). n = 3.