Article

The simple method of distinction between Japanese and Chinese magnolia bark by ion-pair high-performance liquid chromatography using photodiode array detector

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Abstract

To establish a simple method for making distinction between Japanese and Chinese magnolia barks, the components in both magnolia barks were analysed by ion-pair high performance liquid chromatography using a photodiode array detector. Methyleugenol and magnolignan A were detected only in Chinese magnolia barks. The presence or absence, of these components distinguishes Japanese magnolia barks from Chinese magnolia barks. The amounts these components in Chinese magnolia barks were determined.

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Article
To clarify the interspecific and local variations of magnolol, honokiol, and β -eudesmol contents in the magnolia barks, their contents and morphological characteristics of Magnolia obovata, M. officinalis, and M. officinalis var. biloba were studied. The results were as follows. Magnolia officinalis examined had the significantly higher magnolol and honokiol contents than M. obovata and M. officinalis var. biloba. Magnolia obovata had a significantly higher β -eudesmol content than M. officinalis and M. officinalis var. biloba. Additionally, in M. officinalis, a comparatively close correlation was recognized between the magnolol content and the honokiol content, and between the magnolol content and the thickness of bark. Therefore, we propose the ways to choose the Chinese magnolia barks similar to the Japanese magnolia barks in the magnolol content based on the thickness of bark.
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