Effect of feeder cells expressing zebrafish Lif, Fgf2 and Gdnf on 3-week and 6-week FGSC cultures. After 6 weeks of culture, (A) the total number of FGSC colonies significantly decreased on each of the feeder layers when compared to the 3 week cultures; however, the remaining colonies were larger. (B) In the wells containing OFC3LF and OFC3LG feeder layers, the total number of FGSCs increased
... [Show full abstract] approximately 3-fold during this period, and (C) the average number of FGSCs per colony increased 4-fold compared to the 3-week-old cultures. (* indicates the significant difference by Student t tests).
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