Through the moisture absorption and desorption experiments for the 66, 80, 90 and 100 S wool, it gots the moisture absorption and desorption curve and the fitting equation. Through statistical and compute it, the result that higher quality, finer fiber, and the moisture absorption and desorption regain rate were all increased, and the desorption regain rate of each fiber were greater than the absorption regain rate. The finer fibers' rate of the moisture absorption in the initial stage was greater, and through period of time, coarse fibers' moisture absorption rate was greater. In the desorption process, in the initial stage 100 S wool fibers' desorption rate was the slowest, and through period of time it was the fastest.