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Effects of whey peptide extract on the growth of probiotics and gut microbiota

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Whey peptide extract with molecular weight below 1 kDa was investigated in microplate assay, and viable cells, as well as metabolic activity were determined to evaluate augmented growth of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis). Results illustrated that whey peptide extract 1% (w/v) has the capacity to stimulate the proliferation of both probiotic bacteria tested, further supported by the faster generation of metabolic products. The effect of whey peptide extract on the modulation of gut microbiota was also examined in Wistar rats fed either with a standard or a high-fat diet, assessed via 16S ribosomal RNA expression of gut microbiota by quantitative PCR. Relative abundance of Lactobacillus spp., Bifidobacterium spp. and Bacteroidetes was significantly increased by whey peptide extract in rats fed with a standard diet. These results highlight an additional unexploited positive effect of whey peptide extract on gut microbiota modulation.
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Effects of whey peptide extract on the growth of
probiotics and gut microbiota
Ya-Ju Yu a, Manuela Amorim a, Cláudia Marques b, Conceição Calhau b,c,
Manuela Pintado a,*
aCentro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia,
Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal
bDepartamento de Bioquímica, Faculdade de Medicina da Universidade do Porto, Al. Prof. Hernâni Monteiro,
4200-319 Porto, Portugal
cCINTESIS, Centro de Investigação em Tecnologias e Sistemas de Informação em Saúde, Al. Prof. Hernâni
Monteiro, 4200-319 Porto, Portugal
Article history:
Received 23 June 2015
Received in revised form 21 October
Accepted 26 October 2015
Available online
Whey peptide extract with molecular weight below 1 kDa was investigated in microplate
assay, and viable cells, as well as metabolic activity were determined to evaluate aug-
mented growth of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis).
Results illustrated that whey peptide extract 1% (w/v) has the capacity to stimulate the pro-
liferation of both probiotic bacteria tested, further supported by the faster generation of
metabolic products. The effect of whey peptide extract on the modulation of gut microbiota
was also examined in Wistar rats fed either with a standard or a high-fat diet, assessed via
16S ribosomal RNA expression of gut microbiota by quantitative PCR. Relative abundance
of Lactobacillus spp., Bifidobacterium spp. and Bacteroidetes was significantly increased by whey
peptide extract in rats fed with a standard diet. These results highlight an additional
unexploited positive effect of whey peptide extract on gut microbiota modulation.
© 2015 Elsevier Ltd. All rights reserved.
Gut microbiota
Whey peptide extract
1. Introduction
As a major waste of cheese industry, whey has been given in-
creasing importance not only due to its nutritious value but
also due to concern of environmental pollution (Pintado,
Pintado, & Malcata, 1999; Tavares & Malcata, 2013). Whey con-
tains highly valuable soluble milk components, particularly
soluble proteins (0.6–0.8%, w/v) (Barth & Behnke, 1997; Walzem,
Dillard, & German, 2002), and the role of these soluble pro-
teins has been increasingly emphasised due to the discoveries
on their bioactive properties (Sinha, Radha, Prakash, & Kaul,
2007;Urista, Fernandez, Rodriguez, Cuenca, & Jurado, 2011).
By definition, bioactive peptides are certain protein frag-
ments exhibiting positive health impacts (Kitts & Weiler, 2003).
Although bioactive whey peptides are less common than those
of casein, there have been some studies illustrating their diverse
biological functions (Lòpez-Expòsito & Recio, 2006;Marques
et al., 2015; Ortiz-Chao et al., 2009; van der Kraan et al., 2005).
On the other hand, consumption of prebiotics (Grootaert
et al., 2009) to modulate microbiota, such as stimulating pro-
liferation of Bifidobacterium and Lactobacillus genera (Tuohy,
*Corresponding author. Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade
Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal. Tel.: +351 225580097; fax: +351 22 558 00 01.
E-mail address: (M. Pintado).
1756-4646/© 2015 Elsevier Ltd. All rights reserved.
Journal of Functional Foods 21 (2016) 507–516
Available online at
journal homepage:
Rouzaud, Bruck, & Gibson, 2005), which brings about the healthy
impacts (Wang, 2009), has been also increasingly focused.
However, most substances identified as prebiotics or candi-
dates are currently non-digestible oligosaccharides (Crittenden
& Playne, 1996), i.e., the potential of proteins/peptides as
prebiotics, leading to growth promoting effect and improve-
ment of metabolism, has not yet been fully explored.
Various microorganisms exist in the human intestinal tract
and bacteria predominate among them. Advanced methods,
for instance 16S ribosomal RNA (rRNA) investigations and direct
sequencing of faecal microbiota, have facilitated findings on
the relationship between gut microbial communities and host
metabolism. More than 90% of those findings are associated
with Firmicutes and Bacteroidetes (Tremaroli & Backhed, 2012;
Vrieze et al., 2010), and host obesity was found to alter their
colonisation (Furet et al., 2010; Ley et al., 2005). On the other
hand, Actinobacteria (ex. Bifidobacterium) and Proteobacteria (ex.
Helicobacter and Escherichia) also constitute a great number
among gut bacteria (Eckburg et al., 2005; Ley et al., 2005).
In the current study, a new whey peptide (<1 kDa) extract
(WPE) obtained from whey hydrolysed by Cynara cardunculus
was evaluated to determine its impacts on the modulation of
gut beneficial bacteria. Initial screening and conventional mi-
crobiological analysis of the single strains, Bifidobacterium
animalis subsp. lactis (strain Bb 12) and Lactobacillus acidophi-
lus Ki were undertaken to examine in vitro direct effects on
augmented growth of specific bacteria, allowing a more com-
plete understanding of their probable mechanism.Additionally,
animal experiments were also conducted to examine whether
the WPE can modify the composition of colonic microbiota in
two different dietary patterns (standard and high-fat diet), by
quantifying 16S rRNA gene expressions of certain gut microbe
communities through real-time PCR.
2. Materials and methods
2.1. Materials and microorganisms
Fructooligosaccharide (FOS) was purchased from Sigma-
Aldrich (St. Louis, MO, USA). Chemical standards utilised in HPLC
included 98% sulphuric acid (Merck KGaA, Darmstadt, Germany),
glacial acetic acid (Fisher Scientific, Leics, UK), L-(+)-Lactic acid
and glucose (Sigma-Aldrich). Microbial culture media (MRS broth
and MRS agar) were purchased from Biokar Diagnostics (Beau-
vais, France). Lactobacillus acidophilus Ki, previously isolated from
fermented milk, was obtained from CSK Food Enrichment (Leeu-
warden, The Netherlands) as ultrafrozen concentrates, and
Bifidobacterium animalis Bb12 was obtained from Christian
Hansen (Hørsholm, Denmark) as lyophilised culture.
2.2. Preparation of WPE
Whey extracts were obtained and processed according to
Tavares et al. (2012) with some modifications. Mixture of 80%
cow, 10% goat and 10% ewe whey was kindly provided by Saloio
(Torres Vedras, Portugal). Hydrolysis of whey was performed
using aqueous extract of Cynara cardunculus (Formulab, Maia,
Portugal) (Tavares et al., 2011) at an enzyme/substrate ratio of
3.0% (v/v), incubated for 3 h at pH 5.2 and 55 °C. Besides, further
processes were done using a 1 kDa cut-off membrane to gen-
erate the filtrates, constituting the peptides with MW <1 kDa.
The WPE with MW <1 kDa was further used in the following
experiments to assess its probiotic growth enhancing properties.
2.3. Screening of bacterial growth via microplate assay
To evaluate the role of the WPE as prebiotic (assuming the en-
hancing properties upon probiotic bacteria), the microplate
assay was conducted with variable media-pure MRS broth, MRS
broth with 2% (w/v) FOS or different concentrations of the whey
extract (1.0 and 2.0%). MRS broth was autoclaved at 121 °C for
20 min and subsequently supplemented with filter-sterilised
FOS and 0.5 g/L of L-cysteine (for the growth of B. animalis Bb12).
Owing to the lower solubility of whey extract, filter
sterilisation could not be used, but the broth with the extract
had to be sterilised at 80 °C for 30 min. In order to control the
sterility of this medium, a control without inoculum was always
included to prove the absence of growth. Each type of medium
was then inoculated at 2% (v/v) with each of aforementioned
probiotic bacteria. One aliquot of 250 µL was transferred to a
96-well microplate (Thermo Fischer Scientific, Denmark) in trip-
licate and the wells were covered with 50 µL autoclave-
sterilised liquid paraffin (Merck, Germany) to avoid the presence
of oxygen. Incubation occurred at 37 °C for a period of 48 h
under non-controlled pH conditions, and cellular growth was
monitored by measuring the OD of the cultures at 660 nm at
intervals of 60 min using microplate reader (FLUOstar OPTIMA,
BMG LABTECH GmbH, Ortenberg, Germany).
2.4. Evaluation of fermentable activity via determination
of viable cells and metabolic activity
Effect of peptide extracts on the growth of probiotics was evalu-
ated as the following. After completely dissolving, the medium
with the extract was specially sterilised at 80 °C for 30 min as
previously explained. L. acidophilus Ki and B. animalis Bb12 were
inoculated at 2% (v/v) into basal media MRS, with filter-
sterilised FOS 2% (medium used as positive control since FOS
is one of the most used prebiotics) and WPE (1% and 2% w/v)
for 24 h and 48 h, respectively. The culture was then trans-
ferred to 15-mL sterile tubes and incubated at 37 °C for 24 h.
All the conditions were sampled at 8 or 9 time points (0, 2, 4,
6, 8, 10, 12 and 24 h for L. acidophilus Ki and 48 h additionally
for B. animalis Bb12). At each sampling point, inoculated medium
(100 µL) was decimally diluted in salt peptone (1 g/L) (Sigma-
Aldrich), and plated in duplicate on two types of media: MRS
agar for L. acidophilus Ki and MRS agar added with 0.5%
L-cysteine for B. animalis Bb12. Inoculation, incubation and enu-
meration were conducted as described previously by Miles,
Misra, and Irwin (1938).
Of each triplicated sample 500 µL was diluted in 500 µLof
sulphuric acid 13 mM in a single run and the concentration
was obtained based on calibration curves previously pre-
pared with appropriate chromatographic standards. HPLC
analysis was conducted by referring to Zeppa, Conterno, and
Gerbi (2001) in order to assess the metabolic activity of each
probiotic in various medium. The HPLC system consists of a
LaChrom L-7100 pump (Merck-Hitachi, Fullerton CA, USA); an
508 Journal of Functional Foods 21 (2016) 507–516
ion exchange Aminex HPX-87H Column (300 mm ×7.8 mm) (Bio-
Rad, Richmond CA, USA), which was maintained at 65 °C (L-
7350 Column Oven; LaChrom, Merck-Hitachi); UV detector to
analyse organic acids at 220 nm (L-7400 UV Detector; LaChrom,
Merck-Hitachi), and detection was by refractive index for sugars.
The mobile phase used was 13 mM sulphuric acid, at a flow
rate of 0.8 mL/min. The running time was 30 min, and the in-
jection volume was 50 µL. Data were collected and analysed
by a D-7000 Interface (LaChrom, Merck-Hitachi) and HPLC
System Manager 3.1.1 Software (Merck-Hitachi).
2.5. Animals and housing
Twenty-one male Wistar rats (253 ±18 g) were purchased from
Harlan Laboratories (Santiga, Spain) and kept under con-
trolled environmental conditions (22–24 °C and 12 h light/
dark cycles), for at least 1 week before starting the experiments.
Animals were then randomly divided into two different diet
groups: standard diet group (St, N =9) and high-fat diet group
(HF, N =12). Diets were respectively categorised into “Stan-
dard” (Teklad, 2014, Harlan Laboratories) with 48% carbohydrate
(w/w), 14.3% protein (w/w) and 4% lipid (w/w), and “High-Fat”
(D1245 Research Diets, New Brunswick, USA) with 41% carbo-
hydrate (w/w), 24% protein (w/w) and 24% lipid (w/w)). Water
and chow were supplied ad libitum. Food and beverage con-
sumption and body weight were monitored weekly to carefully
characterise energy ingested and weight gain.
After 12 weeks of treatment, animals in each diet group were
further randomly subdivided into two different groups to form
the following 4 experimental groups: St (N =6), St +WPE (N =3),
HF (N =6) and HF +WPE (N =6). WPE was supplied in water at
a dose of 350 mg/kg/day during a total of 5 weeks.
At the end of the study, food was removed 4–6 h before sac-
rifice. Animals were anaesthetised with a mixture of ketamine
(50 mg/kg) and medetomidine (1 mg/kg) and sacrificed by ex-
sanguination. Fresh faecal samples were collected directly from
the colon of all animals, snap-frozen in liquid nitrogen and
stored at 80 °C until further analysis.
Animal handling and housing protocols followed the Eu-
ropean Union guidelines (Directive 2010/63/EU) for the use of
experimental animals in scientific research.
2.6. DNA extraction from stool
Genomic DNA was extracted and purified from stool samples
using NZY Tissue gDNA Isolation Kit (NZYTech, Lisboa, Portu-
gal) with some modifications. Briefly, faeces (170–200 mg) were
homogenised in TE buffer (10 mM Tris/HCl; 1 mM EDTA, pH 8.0)
and centrifuged at 4000 ×g for 15 min. The supernatant was
discarded and the pellet was re-suspended in 350 µL of buffer
NT1. After an incubation step at 95 °C for 10 min, samples were
centrifuged at 11,000 ×g for 1 min. Then, 25 µL of proteinase
K (22 mg/mL) (NZYTech, Lisbon, Portugal) was added to 200 µL
of the supernatant for incubation at 70 °C for 10 min. Con-
secutive steps were adopted according to manufacturer’s
2.7. Microbial analysis of rat stool by real-time PCR
Real-time PCR was performed in sealed 96-well microplates using
a LightCycler FastStart DNA Master SYBR Green Kit and a
LightCycler Instrument (Roche Applied Science, Indianapolis, ID,
USA). PCR reaction mixtures (total of 10 µL) contained 5 µLof
2×FastStart SYBR green (Roche Diagnostics Ltd), 0.2 µLofeach
primer (Sigma-Aldrich, St. Louis, MO, USA) (final concentra-
tion of 0.2 µM), 3.6 µL of water and 1 µL of DNA (equilibrated to
20 ng). Primer sequences used to target the 16S rRNA gene of
the bacteria and the conditions for PCR amplification reac-
tions are described in Table 1 (Bacchetti De Gregoris, Aldred, Clare,
& Burgess, 2011; Delroisse et al., 2008; Queipo-Ortuno et al., 2013;
Stach, Maldonado, Ward, Goodfellow, & Bull, 2003).To verify the
specificity of the amplicon, a melting curve analysis was per-
formed via monitoring SYBR green fluorescence in the
temperature ramp from 60 to 97 °C. Data were processed and
analysed using the LightCycler software (Roche Applied Science).
DNA levels were approximated as 2Ct, where Ctwas the cross-
ing threshold value calculated by the software. Results are
normalised to the universal bacteria gene and expressed in per-
centage of control (St group) (Bose, Song, Nam, Lee, & Kim, 2012).
2.8. Statistics analysis
Results are expressed as mean ±SD. GraphPad Prism 5.02
(GraphPad Software, Inc., La Jolla, CA, USA) was used to analyse
Table1–Primer sequences used for gut microbiota analysis by real-time PCR and corresponded reaction conditions.
Target group Primer sequence (5-3) PCR
Size (bp)
3-step amplification Ref.
Denaturation Annealing Elongation
Bifidobacterium spp. CGCGTCYGGTGTGAAAG 244 95 °C for 10 s 60 °C for 20 s 72 °C for 15 s Delroisse et al. (2008)
Lactobacillus spp. GAGGCAGCAGTAGGGAATCTTC 126 95 °C for 10 s 60 °C for 20 s 72 °C for 15 s Delroisse et al. (2008)
Actinobacteria CGCGGCCTATCAGCTTGTTG 600 95 °C for 10 s 70 °C for 20 s 72 °C for 15 s Stach et al. (2003)
Firmicutes ATGTGGTTTAATTCGAAGCA 126 95 °C for 10 s 60 °C for 20 s 72 °C for 15 s Queipo-Ortuno
Bacteroidetes CATGTGGTTTAATTCGATGAT 126 95 °C for 10 s 60 °C for 20 s 72 °C for 15 s
Universal AAACTCAAAKGAATTGACGG 180 95 °C for 15 s 62 °C for 15 s 72 °C for 20 s Bacchetti De Gregoris
509Journal of Functional Foods 21 (2016) 507–516
all the data. One-way ANOVA followed by Bonferroni’s post-
hoc test was performed to evaluate the significant differences
between groups. Differences were considered significant when
3. Results and discussion
3.1. Screening of probiotic bacteria growth via
microplate assay
In this study, growth curves of L. acidophilus Ki and B. animalis
Bb12 are depicted in Fig. 1. Results from the initial screening
showed that the addition of WPE to the medium can enhance
the growth through different concentrations but contributed
to distinctive outcomes in these two bacteria, i.e., both con-
centrations exerting similar effects on the growth in
L. acidophilus Ki, whereas only WPE 1% led to an obviously im-
proving effect for the growth of B. animalis Bb12. A previous
study found that this bacteria favours more catabolic activity
than anabolic activity at a certain level of nitrogen resource
(Beal & Corrieu, 1995). It is possible that WPE at 2% led to this
shift of physiological activity for B. animalis Bb12 and didn’t exert
positive effect on its growth. Evaluation of its anabolic activ-
ity at this concentration might be done by the detection of
On the other hand, 2% FOS enhanced the growth of
B. animalis Bb12 significantly (P<0.05) after 12 h but had no posi-
tive impact on the growth of L. acidophilus Ki. Due to limited
availability of transport enzyme,intracellular metabolic enzymes
for oligosaccharides, namely glycosyl hydrolases, of lactoba-
cilli have less accessibility to more complex carbohydrates In
comparison, Bifidobacterium spp. developed higher capacity of
hydrolysing extracellularly (Ganzle & Follador, 2012). The lack
of these transport enzymes of L. acidophilus might explain why
no positive impact on growth by FOS was observed.
Hence, it was observed that addition of WPE to the MRS
media significantly (P<0.05) benefited the growth of L. aci-
dophilus Ki and B. animalis Bb12 within 24 h and 48 h,
respectively, and WPE 1% possessed the capacity to improve
growth for both beneficial bacteria.
3.2. Effects of peptides on probiotic bacteria growth and
assessment of metabolic activity
Following the beneficial effects seen in previous screening by
microplate assay, 1% WPE was further tested.
Fig. 2 shows the evolution of pH value and viable cell
numbers (log CFU/mL) at each sampling time for L. acidophi-
lus Ki during 24 h and B. animalis Bb12 during 48 h.
Growth of both probiotics in the media with WPE 1% re-
sulted in significantly lower pH value within the incubation,
possibly benefiting the inhibition of growth of pathogenic bac-
teria (Tannock, 2002).
After the incubation, viable cell numbers of L. acidophilus Ki
showed no significant difference (P>0.05) among three groups,
all achieving ca. 9.7–9.8 log CFU/mL. Nevertheless, the growth
rate of L. acidophilus Ki at exponential phase was still en-
hanced by WPE 1%, whereas it was not the case of FOS 2%.
Regarding the growth of B. animalis Bb12, significantly higher
growth rate and viable cell number after 48 h by WPE 1%
(P<0.05) are shown (Fig. 2) in comparison with control and FOS
2% groups.
Species from the genus Bifidobacterium are said to be the
main consumers prebiotics, are involved in the formation of
acetate and lactic acid (Palframan, Gibson, & Rastall, 2003), and
bring out butyrogenic effect (Falony & De Vuyst, 2009), which
further contributes to bifidogenic effects on the improve-
ment of health (De Vuyst & Leroy, 2001). In addition, lactate
is known to exhibit antimicrobial properties (Koo, Eggleton,
O’Bryan, Crandall, & Ricke, 2012).The production of acetate and
lactic acid by two probiotic bacteria in different media was as-
sessed in this study, not only unveiling the role of WPE in
alteration of metabolic activity but also evaluating its pos-
sible health-promoting property via the induction of organic
Evolutions of organic acids throughout incubation by L. aci-
dophilus Ki and B. animalis Bb12 are presented in Figs. 3 and 4,
respectively.Throughout the incubation of L. acidophilus Ki during
24 h, the production of lactic and acetic acids in the samples
with added WPE 1% exerted the best induction among three
groups. Highest rate (P<0.05) on depletion of the main sub-
strate, glucose, was also shown in the culture of WPE 1%. Due
to its homo-fermentative capacity (Salminen, von Wright, &
Ouwehand, 2004), L. acidophilus Ki preferably produces much
higher lactic acid. Steep decline on the pH value occurring
Fig.1–Growthcurves for (A) L. acidophilus Ki and (B)
B. animalis Bb12. Bacteria were grown in basal MRS
medium (), MRS medium with FOS 2% () and MRS
medium with extract 1% (), 2% (), and growth
determined by measuring the OD values at 660 nm
(mean ±SD of triplicates) over 24 h or 48 h period.
510 Journal of Functional Foods 21 (2016) 507–516
during 4 h to 8 h (Fig. 2) corresponded to the obvious induc-
tion of lactic acid (Fig. 3), causing more significant pH-reducing
effect, in the incubation of L. acidophilus Ki.
Unique metabolic pathway termed “bifid shunt” (De Vries
& Stouthamer, 1967) contributes to higher acetic acid produc-
tion by B. animalis. In accordance with this fact, our result
showed that B. animalis Bb12 generated significantly higher
(P<0.05) acetic acid in the samples with WPE 1% than control
counterpart (Fig. 4). In addition, WPE 1% resulted in the con-
sumption of glucose at higher rate, which metabolised the
substrates to the significantly different (P<0.05) level at earlier
time point (at 4 h).
Aiming to evaluate the properties of WPE as a nitrogen
source, this study utilised the complete MRS basal medium,
i.e., when glucose is present at high concentrations in order
to simulate the real situation. FOS was chosen as the positive
control because no recognised prebiotics that originated from
proteins/peptides have been generally confirmed. FOS 2% trig-
gered higher production of lactic acid for both bacteria but didn’t
enhance the formation of acetic acid for B. animalis Bb12 sig-
nificantly. This may be related to the fact that two sugar sources
are in the media to provide energy constraining the meta-
bolic pathways.
Overall, the supplementation of WPE 1% in the basal MRS
medium was capable of not only enhancing the growth of viable
cells of B. animalis Bb12 but also expediting the metabolism of
both probiotics. Meanwhile, subsequent to the accelerated meta-
bolic process, higher generation of lactic acid mainly from
L. acidophilus and induction of acetic acid from B. animalis were
observed. Some previous observations might lay the founda-
tion to improvement on the growth of probiotics by whey
peptides (Dave & Shah, 1998; Mitchell & Gilliland, 1983), ex-
hibiting health-benefiting effect (Ghasemi-Niri et al., 2011).
Favourable effects of WPE might be attributed to the pres-
ence of amino acids and peptides obtained by hydrolysis of the
proteases from Cynara cardunculus.
3.3. Assessment of the effect of the WPE on the
composition of gut microbiota in rats
After twelve weeks of being fed with an HF diet, animals de-
veloped severe obesity as reflected by a significant increase in
Fig.2–pHvalues (A and B) and viable cell numbers (log CFU/mL) (C and D) (mean ±SD of 6 replicates) for the growth of
L. acidophilus Ki and B. animalis Bb12 incubated for 24 h and 48 h, respectively, at 37 °C growing in basal MRS medium (),
MRS medium with FOS 2% () and MRS medium with extract 1% ().
511Journal of Functional Foods 21 (2016) 507–516
body weight (Marques et al., 2015).To evaluate the effect of WPE
on the modulation of gut microbiota in this diet-induced obesity
model, animals were treated withWPE during five more weeks.
In parallel, animals fed with an St diet were also treated with
WPE for five weeks to evaluate its beneficial bacteria-enhancing
properties in vivo (Table 2).
Progress of metagenomics analyses (Tomas, Langella, &
Cherbuy, 2012) has facilitated the investigation of influential
factors in the human gut microbiota (Gerritsen, Smidt, Rijkers,
& de Vos, 2011; Turnbaugh et al., 2009). Among them, a strong
correlation between diet and gut microbiota has been estab-
lished (Fallani et al., 2010; Sekirov, Russell, Antunes, & Finlay,
2010). It has been shown that obesity induced by HF diet modi-
fied the profile of intestinal bacteria (Cani et al., 2007;Kim, Gu,
Lee, Joh, & Kim, 2012), i.e., decreasing the relative abundance
of Bacteroidetes (Turnbaugh et al., 2006) and consequently in-
creasing Firmicutes to Bacteroidetes ratio (Ley, Turnbaugh, Klein,
Fig. 3 – Lactic and acetic acids (g/L) produced and glucose
(g/L) consumed by L. acidophilus Ki throughout 24 h of
incubation at 37 °C. (A, B and C) Differences (P<0.05)
among control (MRS broth ), FOS 2% () and extract 1% ()
at each sampling time (mean ±SD of 6 replicates). (a–e)
Differences (P<0.05) in each sample throughout time
(mean ±SD of 6 replicates).
Table 2 – Energy ingestion of animals from different experimental groups during the 5 weeks of the study.
St St +Whey HF HF +Whey
Energy ingestion (kcal/day) 53.3 ±7.4 56.3 ±3.9 74.2 ±8.4*69.4 ±7.3*
Values are expressed as mean ±SEM.
*P<0.05 vs St group.
Fig. 4 – Lactic and acetic acids (g/L) produced and glucose
(g/L) consumed by B. animalis Bb12 throughout 48 h of
incubation at 37 °C. (A, B and C) Differences (P<0.05)
among control (MRS broth ), FOS 2% () and extract 1% ()
at each sampling time (mean ±SD of 6 replicates). (a–e)
Differences (P<0.05) in each sample throughout time
(mean ±SD of 6 replicates).
512 Journal of Functional Foods 21 (2016) 507–516
Fig. 5 – Real-time PCR quantification of microbiota phyla (A) Actinobacteria, (B) Firmicutes, (C) Bacteroidetes and genera (D)
Bifidobacterium spp., and (E) Lactobacillus spp. in different groups of treatment. (F) Firmicutes to Bacteroidetes ratios were
calculated as the relative expression of 16S rDNA of Firmicutes divided by the one of Bacteroidetes. Data are presented in
percentage of control as mean ±SD (N =3–6). *P<0.05, **P<0.01 and ***P<0.001 vs St group. St, standard diet group; HF,
high-fat diet group; WPE, whey protein extract.
513Journal of Functional Foods 21 (2016) 507–516
& Gordon, 2006). These alterations in the gut microflora com-
position are associated with an increased intestinal permeability
that eventually can lead to the development of metabolic
endotoxemia, inflammation and metabolic disorders (Cani et al.,
In the present study, after seventeen weeks, HF diet caused
a significant decrease in the relative abundance of Bacteroidetes,
in comparison with the St group. It also led to induction on
the ratio of Firmicutes to Bacteroidetes (P<0.05). With regard
to the relative abundance of Actinobacteria, Lactobacillus spp.,
Bifidobacterium spp. and Firmicutes, no differences were ob-
served between St and HF group (Fig. 5). Results from our group
also indicated that a similar pattern is observed in the gut
microbiota of rats fed with a high fat diet for twelve weeks
(Marques et al., 2015).
Under the pattern of St diet, WPE significantly increased the
relative abundance of Bacteroidetes (P<0.05), Bifidobacterium
spp. (P<0.05) and Lactobacillus spp. (P<0.001). However, no sig-
nificant changes were observed in the Firmicutes to
Bacteroidetes ratio and in the relative abundance of
Actinobacteria and Firmicutes, in rats treated withWPE (Fig. 5).
On the whole, in a diet-induced obesity model,WPE did not
cause any statistical significant change in the relative abun-
dance of above mentioned bacteria compared with HF control
group. These results showed that WPE was not able to rescue
the deleterious outcomes caused by HF diet through raising
the number of beneficial bacteria. Since WPE supplementa-
tion was only initiated after obesity was installed, these findings
might indicate that a longer or earlier intervention may be re-
quired for WPE to modulate intestinal flora.
Nevertheless, the effects of WPE in the presence of an St
diet are remarkable.WPE promoted the growth of Bifidobacterium
spp. and Lactobacillus spp. in vivo like our in vitro studies had
pointed out. These results are in accordance with a previous
study conducted in pigs that reported a larger population of
lactobacilli and bifidobacteria in gut microbiota after liquid whey
feeding (Kobayashi et al., 2011). Furthermore, WPE also in-
creased the relative abundance of Bacteroidetes.
Effects of bioactive peptides incorporated into diet either
are required to resist gastrointestinal digestion or undergo
physiological conversion into functional fragments, ex. the
case of some antihypertensive peptides (Miguel, Aleixandre,
Ramos, & Lopez-Fandino, 2006; Vermeirssen, Van Camp, &
Verstraete, 2004), and reach target sites to exert its bioactivi-
ties. In our study, despite that both preliminary in vitro
assessments and the following in vivo evaluation on microbiota
couldn’t specifically indicate which mechanism WPE con-
ducts, the benefits acting in the organisms were demonstrated.
It is still necessary that the identification and characterisa-
tion of bioactive peptides in WPE are executed in the future
It is known that Bacteroides spp. belongs to one of amino
acid fermenting bacteria (Dai, Wu, & Zhu, 2011) and is able to
secrete proteolytic enzymes in large intestine, which is also
attributed to Lactobacillus spp. and Bifidobacterium spp. (Macfarlane
& Cummings, 1991). This, combined with other features, in-
cluding polyamine production (Allison & Macfarlane,1989) and
aromatic amino acid fermentation (Smith & Macfarlane, 1996),
may explain the distinctive growth of these three species by
WPE in our gut microbiota analysis.
Altogether, these findings highlight for the first time the pos-
sible intervention of WPE as a probiotic growth and metabolism
enhancer – “prebiotic” in a healthy life style.
4. Conclusions
More innovative than other hydrolysis processes, whey extract
evaluated in this study was obtained by the enzymatic reac-
tions of proteases from Cynara cardunculus, which is vegetable
rennet, instead of the enzymes originated from animal or mi-
croorganism. After some in vitro assessments, the fraction of
WPE with MW <1 kDa at 1% showed the potential to be used
as a supplementation to promote the growth of Lactobacillus
acidophilus Ki and Bifidobacterium animalis Bb12 by lowering pH
and inducing faster organic acid production, including lactic
acid and acetic acid.
Through the investigation of rat gut microbiota commu-
nity in this study, it was observed that intake of WPE at the
dose of 350 mg/day/kg in rats can significantly induce the 16S
rRNA expression of Lactobacillus spp., Bifidobacterium spp. and
Bacteroidetes in gut under the pattern of St diet, meaning the
shift towards the beneficial microbiota profile, but was not
capable of rescuing the adverse consequences resulting from
The production of WPE was supported by ACTIPEP Project
(QREN-ADI 11531). This work was supported by National Funds
from FCT – Fundação para a Ciência e a Tecnologia through
project PEst-OE/EQB/LA0016/2013 and by the PhD grant of Maria
Manuela Amorim and Cláudia Marques with reference SFRH/
BD/81901/2011 and SFRH/BD/93073/2013, respectively.
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... In addition to carbon-sourced prebiotics, protein hydrolysates or peptides with potential prebiotic functions were also reported to play an important role in promoting the growth of lactic acid bacteria, thereby inhibiting intestinal pathogens (Sanders, Merenstein, & Merrifield, 2018). Whey protein can specifically increase the amount of L. rhamnosus in the rat intestine, inhibit the adhesion of pathogenic bacteria, promote the secretion of antibacterial substances by a variety of lactic acid bacteria, and inhibit the growth of a variety of pathogenic bacteria (Bartkiene et al., 2019;Yu, Amorim, Marques, Calhau, & Pintado, 2016). Pea protein hydrolysates and buckwheat protein hydrolysates also showed the ability to promote the adhesion of Lactobacillus to intestinal epithelial cells (Świątecka, Markiewicz, & Wróblewska, 2012. ...
... During the period of 8 h to 12 h, the viable cells in the dpep group were significantly higher than those of the pep group and dpro group. At 12 h, the viable count of the dpep group reached the highest value of 7.27 × 10 9 CFU/mL, which inferred that L. reuteri LR08 can make better use of small molecular peptides for growth and reproduction, which was consistent with the activity of the small molecular peptides identified from soybean and whey (Capriotti et al., 2015;Yu et al., 2016). The difference is that in the results of growth curve, pep group and dpro group were lower than MRS group, but not lower than MRS group in the determination of viable count, suggesting that the ratio of viable count of L. reuteri was higher in the pep group and dpro group. ...
... Izaguirre, Dietrich, Villarán, and Castañón (2020) reported that the fermentative production of lactic acid usually requires large quantities of nitrogen and carbon. Some previous observations might also lay the foundation for the improvement of organic acid secretion by proteins or peptides (Fitzpatrick & O'Keeffe, 2001;Yu et al., 2016). However, the mechanisms by which proteins and peptides, as nitrogen-sourced nutrients, regulate organic acid production in Lactobacillus still need further investigation. ...
Soybean peptides were reported to promote the growth and metabolism of Lacticaseibacillus rhamnosus (L. rhamnosus) Lra05. However, the relationship between L. rhamnosus Lra05 and the characteristics of soybean peptides is still unclear. Therefore, digested soybean peptides (dPEP) after 36 h utilization by L. rhamnosus Lra05 were identified and analyzed. We found that L. rhamnosus Lra05 tends to utilize hydrophobic peptides with three to five amino acids residues, and hydrophilic peptides with more than five residues. They also prefer peptides with proline at penultimate C-terminal position or arginine at ultimate C-terminal position. Moreover, fraction 1 (F1) and fraction 7 (F7) acquired from dPEP using RP-HPLC exhibited the strongest growth and metabolism promoting effects, and the utilized characteristics of F1 and F7 were similar with those of dPEP. These results explained why soybean peptides could promote L. rhamnosus to some extent and strengthen theoretical basis for the application of soybean peptides as potential prebiotics.
... At the genus level, the Akkermansia, Allobaculum, Bacteroides, Peptococcus, Bifidobacterium, Parasutterella, Saccharibacteria, and Clostridium were identified, among which, Akkermansia, Allobaculum, Bacteroides were the main genus in all groups. The Akkermansia is demonstrated to be a promising probiotic candidate for gut health and a decreasing abundance is revealed in IBD patients [29]. In the comparison between NC and PC group (Figure 9B), the Akkermansia also had significant differences where the DSS (18.7) posed to have lower abundance than ...
... At the genus level, the Akkermansia, Allobaculum, Bacteroides, Peptococcus, Bifidobacterium, Parasutterella, Saccharibacteria, and Clostridium were identified, among which, Akkermansia, Allobaculum, Bacteroides were the main genus in all groups. The Akkermansia is demonstrated to be a promising probiotic candidate for gut health and a decreasing abundance is revealed in IBD patients [29]. In the comparison between NC and PC group ( Figure 9B), the Akkermansia also had significant differences where the DSS (18.7) posed to have lower abundance than NC (26.8%). ...
... In the current study, the relevant abundances of major gut microbiota were analyzed in phylum and gene levels. The Akkermansia is demonstrated to be a promising probiotic candidate for gut health and a decreasing abundance is revealed in IBD patients [29]. As expected, the GP intervention improved the Akkermansia abundance. ...
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The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension. In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice. The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues. In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment. In the analysis of gut microbiota, the GP was checked to modulate the abundance of Akkermansia, Parasutterella, Peptococcus, Bifidobacterium and Saccharibacteria. The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.
... More recently, an IF with partially hydrolyzed WP was proved to support adequate growth for healthy term infants [6]. WP hydrolysate was also found to serve as a probiotic growth and metabolism enhancer for Wistar rats, which were fed with a standard diet [7]. However, few studies have been conducted to evaluate the potential effect of hydrolyzed WP on the infant gut microbiome. ...
Full-text available
Whey protein and its hydrolysate are ubiquitously consumed as nutritional supplements. This study aimed to evaluate the potential effect of whey protein hydrolysate (WPH) on the infant gut microbiome, which is more variable than that of adults. Colonic fermentation was simulated through a static digestion model and fecal culture fermentation, using feces from normal infants aged from 1–3 years old. During in vitro gut fermentation, measurements of short-chain fatty acids (SCFA) concentrations and 16S rRNA amplicon sequencing were performed. Additionally, the growth curves of cultivated probiotics were analyzed to evaluate the prebiotic potential of WPH. Besides the decline of pH in fermentation, the addition of WPH induced a significant increase in the SCFA production and also the relative abundance of Proteobacteria, Bacteroides, and Streptococcus (p < 0.05). The lower ratio of Firmicutes/Bacteroidetes in WPH-supplemented samples indicated the positive modulation of WPH on the gut microbiota, which could benefit the energy balance and metabolism of infants. The stimulation effect of WPH on the probiotics (particularly Lactobacillus acidophilus NCFM) during cultivation implied the prebiotic potential as well. Our findings shed light on WPH as a valuable dietary supplement with not only enriched resources of essential amino acids but also the potential to restore the infant gut microbiome.
... The relative abundance of Coprococcus and Clostridium was increased and the SCFAs production was also elevated, revealing the potential of GMP to improve the host's gut microbiota (Ntemiri et al., 2017). The whey protein-peptide, which was also derived from milk, has been proven to promote the growth of lactic acid bacteria in the Wistar rat (Yu, Amorim, Marques, Calhau, & Pintado, 2016). In addition, dietary egg albumin peptides could change the gut microbiota of Zucker obese rats, and the composition of the gut microbiota in rats after twelve weeks of intervention was similar to that of the lean control group (Requena et al., 2017). ...
In this study, a twelve-week intervention was conducted to investigate the anti-obesity effects of yak bone collagen hydrolysates (YBCH) on mice with a high-fat diet. The obesity-associated phenotypes of mice were detected and the feces of mice were jointly analyzed by 16S rRNA gene sequencing and untargeted metabolomics. Results indicated that supplementation with YBCH could ameliorate the obesity-associated phenotypes of mice, especially with the medium dose (MD) and high dose (HD) of YBCH. Compared with the high-fat diet control (HC) group, the ratio of Firmicutes and Bacteroidetes in the fecal microbiota of the low dose (LD), MD, and HD groups was separately decreased by 29.83 %, 70.85 %, and 75.70 %. Lachnospiraceae and Muribaculaceae were the key bacteria for the YBCH intervention, which might be attributed to their different substrate preferences. The joint analysis of the 16S rRNA gene sequencing and untargeted metabolomics suggested that the anti-obesity effects of YBCH might be achieved by up-regulating the arginine and proline metabolism and down-regulating the aromatic amino acids metabolism via the gut microbiota.
Modern food diets primarily rely upon the extensive usage of the meal or refined flour of bread wheat. Some individuals revealed the immunogenic response after eating food products made from gluten-containing cereals (wheat, barley, and rye), which is known as immune-mediated enteropathy or coeliac disease (CD). The specific category of gliadins and glutenins is responsible for such immunogenic reactions. Immune epitope database (IEDB) analysis revealed the presence of 190 T-cell stimulatory epitopes for celiac disease in wheat. Diarrhea, abdominal pain, fatigue, bone pain, weakness are the primary symptoms of CD. Therefore, CD patients are advised to exclude wheat flour from the diet and rely upon the composite flours from pseudo-cereals, pulses, etc., in routine diets. Gluten-free bread and muffins are usually made from starch and deficient in proteins, minerals, and dietary fibres. The lower gas retention capacity of gluten-free dough yields hard texture bread with lower loaf volume. Poor texture attributes of crust and crumb, and other sensory properties such as aroma and crust color, poor mouth feel decreases the consumer acceptability of gluten-free products. A high rate of starch syneresis after baking was attributed to the lower shelf-life of the gluten-free products. Therefore, the enhancement in the texture properties of gluten-free products by using non-glutenous proteins with higher nutritional values is the major challenge. The reduction in the glycaemic index of non-glutenous baked food products by the utilization of starches from gluten-free cereals/pulses is also required. Therefore, the new formulations based on composite flours that gluten-free products possessing sensory quality attributes comparable to those from wheat, rye, and barley are gaining global interest. In the present chapter, the functional characteristics of alternative sources of wheat for the processing of gluten-free products are discussed.KeywordsCoeliac diseasePseudocerealsGluten-free productsFunctional foodGlycemic index
This work proposes an innovative approach to valorise swine blood based on enzymatic hydrolysis and membrane fractionations. Hydrolysis with Cynara cardunculus enzymes, followed by microfiltration and double nanofiltration generated three high protein fractions, retentate of microfiltration (RMF; >0.5 µm) and retentate of nanofiltration (RNF; >3 kDa) with approximately 90% of protein on a dry basis and filtrate of nanofiltrate (FNF; <3 kDa) with 65%. FNF, rich in low molecular weight peptides, showed excellent antioxidants (ABTS and ORAC of 911.81 and 532.82 µmol TE g⁻¹ DB, respectively) and antihypertensive (IC50 of 28.51 µg mL⁻¹) potential. By peptidomics and in silico analysis, 43 unique sequences of interest were found, among which LVV-Hemorphin-7 was identified. This hemorphin was demonstrated as the main responsible for the observed bioactivity. Complementary results showed a prebiotic effect mainly for the growth of Bifidobacterium animalis Bo, as well as interesting free amino acids (mainly glutamic acid, leucine, alanine, phenylalanine and aspartic acid) and mineral (e.g., Ca, Mg, P, K and Na) profiles. No antibacterial effect was verified for the seven pathogenic bacteria tested. This study allowed obtaining new ingredient of high nutritional and nutraceutical value for human consumption, with a perspective of sustainability and industrial viability.
The object of the study is a probiotic Rumit produced by Biotrof Ltd., which can be added to the diet of milk-fed calves. It is developed on the basis of cellulolytic bacteria. In order to assess the effectiveness of the probiotic use there has been conducted a scientific and agricultural experiment on the basis of the agricultural production co-operative Kolkhoz Andoga of the Kaduysky district, Vologda region since March to June 2021. Studied were 20 calves of black-and- white bred aged 1.5-2 months divided according to the principle of paired peers into two groups: a control and an experimental. The experimental group was given the probiotic Rumit in the amount of 15 g/head/day during 90 days as a supplement to the basic diet. The data on live weight obtained in the course of the study indicate that the use of the probiotic in feeding calves has led to an increase in gross growth to 74.2 kg, that has provided a 3.8 % reduction in feed costs per unit of output.
Bioactive peptides derived from diverse food proteins have been part of diverse investigations. Whey is a rich source of proteins and components related to biological activity. It is known that proteins have effects that promote health benefits. Peptides derived from whey proteins are currently widely studied. These bioactive peptides are amino acid sequences that are encrypted within the first structure of proteins, which required hydrolysis for their release. The hydrolysis could be through in vitro or in vivo enzymatic digestion and using microorganisms in fermented systems. The biological activities associated with bio-peptides include immunomodulatory properties, antibacterial, antihypertensive, antioxidant and opioid, etc. These functions are related to general conditions of health or reduced risk of certain chronic illnesses. To determine the suitability of these peptides/ingredients for applications in food technology, clinical studies are required to evaluate their bioavailability, health claims, and safety of them. This review aimed to describe the biological importance of whey proteins according to the incidence in human health, their role as bioactive peptides source, describing methods, and obtaining technics. In addition, the paper exposes biochemical mechanisms during the activity exerted by biopeptides of whey, and their application trends.
Entomophagy is the practice of eating insects, and inclusion of edible insects in the diet has been followed by humans for many years, especially among some of the ethnic groups of South America, Mexico, Africa, and Asia. Entomophagy is considered a potent dietary practice to replace animal protein due to its highly nutritious, protein-rich, and environmentally sustainable nature. Beneficial effects of edible insects on health have been reported in numerous studies, and they possess several health-promoting properties such as antidiabetic, antioxidative, antiobesity, and anticancer activity, along with Gastro-intestinal health benefits. Apart from major nutrients, edible insects are a rich source of fiber (approx. 2%–8% dry weight basis). Chitin, a polymer of N-acetyl-d-glucosamine, is a major component of the exoskeleton of respiratory linings, digestive, and excretory systems of arthropods and is also resistant to mammalian digestive enzymes. This chapter covers the effect of edible insects on gut health and other disease conditions owing to their richness in fibers and chitins. The food products enriched or supplemented with edible insects and their components may help combat nutritional deficiency and reduce disease incidence. The consumer behavior toward entomophagy along with the potential risks of entomophagy is also described here.
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In the past decades, obesity and associated metabolic complications have reached epidemic proportions. For the study of these pathologies, a number of animal models have been developed. However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. Wistar and SD rats were assigned for 2 experimental groups for 17 weeks: standard (St) and high-fat (HF) diet groups. To assess some of the features of the metabolic syndrome, oral glucose tolerance tests, systolic blood pressure measurements and blood biochemical analysis were performed throughout the study. The gut microbiota composition of the animals of each group was evaluated at the end of the study by real-time PCR. HF diet increased weight gain, body fat mass, mesenteric adipocyte's size, adiponectin and leptin plasma levels and decreased oral glucose tolerance in both Wistar and SD rats. However, the majority of these effects were more pronounced or earlier detected in Wistar rats. The gut microbiota of SD rats was less abundant in Bacteroides and Prevotella but richer in Bifidobacterium and Lactobacillus comparatively to the gut microbiota of Wistar rats. Nevertheless, the modulation of the gut microbiota by HF diet was similar in both strains, except for Clostridium leptum that was only reduced in Wistar rats fed with HF diet. In conclusion, both Wistar and SD Rat can be used as models of HF diet-induced obesity although the metabolic effects caused by HF diet seemed to be more pronounced in Wistar Rat. Differences in the gut microbial ecology may account for the worsened metabolic scenario observed in Wistar Rat.
While lactic acid producing fermentation has been utilized to improve the storability, palatability, and nutritive value of perishable foods for a very long time, only recently have we begun to understand just why it works. The first edition of this international bestseller both predicted and encouraged vigorous study of various strains of lactic acid bacteria in order to substantiate much of what was known but never scientifically validated. The editors now feel compelled to offer a new edition in order to inform microbiologists, food technologists, nutritionists, clinicians, product development experts, and regulatory experts of what continues to be rapid progress in the field. Lactic Acid Bacteria: Microbiological and Functional Aspects, Third edition brings its readers up to date on this continually expanding branch of study incorporating the latest research and findings from all corners of the world. In keeping with the inclusive multidisciplinary philosophy of the original editions, the editors have recruited several more scientists of distinction -- bringing the total of contributors to 37. The bulk of the text has been completely rewritten with new chapters being added to cover recently evolved topics, such as mathematical modeling, vegetable fermentation, methods for analysis of the gut microbiota, and probiotics for fish. As in the two previous editions, the present volume gives a valuable overview of the present status of this rapidly expanding interdisciplinary area of research. Also, as before, special emphasis has been placed on the health aspects of lactic acid bacteria, although, as can be seen in the table of contents, other relevant applications are covered as well.
Deriving from positive effects of whey drinking cures in antiquity, the Middle Ages and modern time, a review is given on nutritional significance of whey. The proteins are essential components of whey and belong to the proteins with highest biological value because of their amino acid composition. Besides, they show fundamental functional properties, which enable a varied application in foods, dietetic foods and beverages in form of different whey products (powder, protein concentrates and isolates). Whey proteins have found considerable usage in infant's nutrition as whey predominant formulas as well as whey protein hydrolysates in case of cow's milk protein intolerances. A recent field of research are biological active peptide sequences which become effective during digestion and are of importance for secretion of entero hormones as well as for immune enthancing effects. They may contribute to assess the biological value of whey proteins under enlarged points of view and to develop new application forms and areas. It is pointed to further fields of application (e. g. adipositas, gout, kidney insufficiency). Concerning the quantitatively most dominant lactose in whey, it is dealt with its importance for the healthy development of infants (adaptation to the increased lactose content of mother's milk) as well as with lactose intolerance and galactosaemia. In case of mineral salts of whey it is emphasized the high nutrient density of calcium (prophylaxis for osteoporosis), the beneficial Ca : P and Na : K proportions (antihypertensive in case of the last one), the promotion of absorption of mineral salts by lactose, and the high content of iodine. The whey is rich in B-vitamins, which contribute essentially for their satisfaction or requirement in case of a corresponding consumption. To be emphasized is the vitamin B12 in milk and whey, which is the sole source of this indispensable nutrient for blood-formation and cell division in lacto-ovo-vegetarian nutrition. In conclusion, a summerizing dietetics valuation of whey is performed.