In pot experiments, the effect of single and combined pollution of soil by lead (Pb), cadmium (Cd), and zinc (Zn) and uptake of heavy metals in Brassica napus L. were investigated. There were two main factors that affected the assimilation of Pb, Cd, and Zn by rape: (i) level of soil pollution by the particular element and (ii) the combined influence of Pb, Cd, and Zn. In general, with the ... [Show full abstract] increase of the concentrations of Pb, Cd, and Zn in the soil, there were increases in the concentrations of those elements in the roots, stems, and seeds. The main part of the Pb and Zn amounts taken up by the roots from the soil are fixed and accumulated in the roots, and small amounts of them move through the conductive system to the seeds. Cadmium moves relatively easily from root to stem and is accumulated in higher concentrations in the top of the plant. There is a well-expressed synergistic interaction between Pb2+ and Cd2+, as well as of Cd2+ and Zn2+. Zinc has a highly depressing effect on the assimilation of Cd2+, as does Pb2+ on the assimilation of Zn2+. The combined pollution by Pb, Cd, and Zn stimulated the assimilation of these elements by the roots and foliage and eliminated the effect of Zn2+ on Cd2+ and of Pb2+ on Zn2+.