The data used for calibration of a water distribution system (WDS) model contain uncertainties. These uncertainties include, in particular, errors in measurements of pressures and water flows, errors in demands, demands pattern, erroneous node elevations, limited sample size, etc. Moreover, some assumptions influence the results of calibration as well. For example, water inflow into WDS is often higher than sum of registered demands. This difference between water inflow and demands includes real and apparent leakages with unknown proportionality. The results of calibration depend on the assumed proportion between real and apparent leakages and on the assumed distribution of them over WDS. Weight coefficients, assumed for pressure and flow measurements in objective function, used in the optimization procedure, are another assumption influencing calibration results. WDS models contain also patterns of demands. But real demands are usually measured for quite long periods of time (e.g. a month) and give information only on the average base demand. Experience in the calibration of WDS models showed large uncertainties of hourly demands estimated by this manner. The effect of these types of uncertainties on calibration results has been considered in the paper.