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Evaluation of the potential effect of the Fir Siberian essential oil modulator of proteolytic activity of Candida albicans isolated from several biological materials

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Abstract

Introduction: Many studies show antifungal activity of several essential oils of plant such as Fir Siberian essential oil. However there is no investigation how can Fir Siberian essential oil modulate the hydrolytic activity of fungal enzymes. The aim of study: Evaluation of the potential effect of the Fir Siberian essential oil as a modulator of proteolytic activity of Candida albicans isolated from several biological materials. Material and methods: 24 Candida albicans strains were isolated from patients of Medical University Hospital no 1 in memorial N. Barlicki in Lodz and from patients who were treatment in hospital's ambulatory. Candida were isolated from: pharyngeal mucosa (6 strains), tongue (1 strains), peritoneal fluid (3 strains), sputum (4 strains), ear secretion (1 strains), urine (4 strains), stools (2 strains), blood (1 strains) and after-surgery wound secretion (2 strains). The hydrolytic activity Candida albicans was estimated by using API ZYM test (bioMérieux). The strains were cultured without (control) and in the presence of the Fir Siberian essential oil. The hydrolytic activity of Candida was examined for minimal inhibitory concentration of essential oil, measured by the diffusion in agar gel method (Kadłubowski). Results: In strains which were cultured without the Fir Siberian essential oil (control) observed activity of 15 from 20 enzymes: no. 2 - alkaline phosphatase, no. 3 - esterase (C4), no. 4 - esterase lipase (C8), no. 6 - leucine arylamidase, no. 7 - valine arylamidase, no. 8 - cystine arylamidase, no. 9 - trypsin, no. 10 - α-chymotrypsin, no. 11 - acid phosphatase, no. 12 - naphtol-AS-BL- phosphohydrolase, no. 15 - β-glucuronidase, no. 16 - α-glucosidase, no. 17 - β-glucosidase, no. 18 - N-acetyl-β-glucosylamidase, no. 19 - α-mannosidase. Candida albicans strains showed no hydrolytic activity for enzymes: no. 5 - lipase (C4), no. 13 - α-galactosidase, no. 14 - β-galactosidase, no. 20 - α-fucosidase. The activity of leucine arylamidase (x= 4.04) and esterase (C4) (x = 3.25) was the highest. The activity of enzymes: trypsin and β-glukosidase was the lowest (x=0.08). Incubation with the Fir Siberian essential oil caused that examined strains released only 7 hydrolases: no. 3 - esterase (C4), no. 4 - esterase lipase (C8), no. 6 - leucine arylamidase, no. 11 - acid phosphatase, no. 12 - naphtol-AS-BL- phosphohydrolase, no. 16 - α-glucosidase, no. 18 - N-acetyl-β- glucosylamidase. No hydrolytic activity showed for 12 hydrolases: no 2, 5, 7, 8, 9, 10, 13, 14, 15, 17, 19, 20. After the incubation with oil the activity of leucine arylamidase and esterase (C4) was the highest but compared to control was lower (x=1.80) and (x=1.70). The Fir Siberian essential oil inhibited releasing 8 hydrolases from examined strains: no 2, 7, 8, 9, 10, 15, 17, 19. Conclusion: The oleum pichtae reduced the hydrolytic activity Candida albicans and can caused their lower virulence. The inhibition effect of the Fir Siberian essential oil of hydrolytic activity Candida can be recommended as the agent for the treatment of candidosis.

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