Defibrillation overload recovery performance is one of the most important electrical properties of ECG electrodes, which directly affects the ECG display after the patient is defibrillated, thereby affects the diagnosis and treatment of the patient. A defibrillation overload recovery performance test system for ECG electrodes was developed according to the latest pharmaceutical industry standards and based on the C8051F340 hardware system. With USB protocol data transfer, the detection of defibrillation overload recovery performance parameters (including AC impedance) is implemented on LabVIEW software platform. The system has good electrical isolation control, which can accurately operate the charging and discharging time during test process automatically. Experiment result shows that this system has good performance, and features safe, accurate and easy to operate.