An investigation was conducted on two consecutive years during 2016-17 & 2017-18 to study the response of cultivars, different spacing and time of fertigation on parthenocarpic cucumber (Cucumis sativus L.) under naturally ventilated polyhouse condition at Krishi Vigyan Kendra, Raisen (M.P). The whole experiment was arranged 12 treatments consisting of three cultivars V1-Kafka, V2-Kian and V3-Hilton along with two levels of spacing S1-50 × 30 cm, S2-50 x 50 cm and 2 levels of fertigation F1-once in a week, F2-once in a 10th days interval (recommended dose of N P K-8:3.5:9 kg/500 m 2. The experiment was laid out in factorial completely randomized design with three replications. The results showed that all vegetative characters under study were significantly influenced by various cultivars along with levels of spacing and time of fertigation. The effects of cultivars, spacing and time of fertigation were significant for most of vegetative characteristics except weight/fruit. The variety Kafka was found to be significantly superior in respect of yield and yield attributing traits having maximum values of 2.78 kg and 14.92 kg yield per vine and per square meter, respectively. The characters like vine length, intermodal distance, leaf area, days to flower bud initiation, fruit set (%), fruit drop (%), no. of fruit per vine, fruit length, fruit width, yield per vine and yield per m 2 were significantly influenced by interaction effect of variety, spacing and time of fertigation. The treatment of V1S1F1 registered significantly highest vegetative and yield attributing characters. However, treatment combination of V3S2F2 registered lowest yield per vine and per square meter (1.43 and 5.73 kg, respectively). Greenhouse cucumber production is very popular in many areas of the world. The cucumber is a warm season crop with required growing conditions of 80°F and plenty of sunlight. The fruits are mild in flavor, seedless, and have a thin edible skin that requires no peeling. Greenhouse cucumber cultivars are parthenocarpic (produce fruit without pollination). Gynoecious cultivars, i.e., those producing only female flowers or predominately gynoecious cultivars should be grown. India, being a vast country with diverse and extreme agro climatic conditions, the protected cultivation technology can be utilized for year round production of high value quality vegetable crops, with high yield. Protected cultivation has higher water and nutrient use efficiencies. Increasing photosynthetic efficiency and reduction in transpiratory losses are added advantages of protected cultivation. Both of these factors are of vital importance for healthy and luxuriant growth of crop plants. This technology is highly suitable for farmers in peri-urban areas of the country, especially in central zone of India. But protected cultivation requires. Careful planning and attention including selection of varieties, suitable production technology like spacing, time of planting, water and nutrient management and plant protection to produce economic yield of good quality. The cultivars of cucumber require a constant water supply along with fertilizer application to reach high quality yield. The irrigation and fertigation system of cucumbers under naturally ventilated greenhouse through drip system of irrigation and fertilizers are also applied along with irrigation water according to the crop growth and season of cultivation. In view of importance of protected cultivation, the study was initiated to find out suitable cultivar, plant spacing and time of fertigation (fertilizers with irrigation water) in cucumber grown under naturally ventilated polyhouse.