Objective: To study the effect of dorsal penile nerve selective neurectomy on the sensitivity of the penile before and after surgery. Methods: The penile vibration thresholds of penils in 92 cases of patients with primary premature ejaculation who underwent the dorsal penile nerve selective neurectomy were detected before operation and a a month and 3 months fter operation. Results: The glans penis vibration thresholds (GPVT) was (0.048±0.015) μm before operation, (0.280±0.084) μm 1 month after operation, (0.282±0.081) μ m 3 months after operation; The penile shaft vibration thresholds (PSVT) was (0.061±;0.014) μ m before operation, (0.064±0.022) μ m 1 month after operation, (0.065±0.020) μ m 3 months after operation.Statistical analysis showed: (1)GPVT at a month after operation significantly higher than that before operation(P<0.05). (2)GPVT at 3 months after operation was significantly higher than before operation (P <0.05). (3)Between one month after operation and 3 months after operation non significant change was found in GPVT(P> 0.05). (4)No significant change was found in PSVT preoperative and postoperative (P> 0.05). Conclusion: 1, Detection of penile vibration thresholds is simple, noninvasive, quantitative, reproducible methods for dorsal penile nerve sensitivity of objective testing methods. 2, After dorsal penile nerve selective neurectomy ,the sensitivity of the glans penis decreased and the threshold level become higher. 3, Dorsal penile nerve selective neurectomy is safe and effective for the treatment of primary premature ejaculation.