Many years of trials and research tests proved that a lot of well-known vitamins could be successfully used in cosmetology. The available data indicate that one of them - folic acid plays an important role in life process of mitotically active tissues and its deficiency increases background level of DNA damage. Folic acid seems to have skin regeneration properties and it can modulate DNA repair in UV-damaged skin. In this study we check the possibility of using the folic acid (folacin) in personal care products and cosmetics as an anti-photo-aging cosmetic ingredient. At first in vitro research was performed on primary fibroblast culture: cell viability was determined in a Burker chamber; the shape of the cells was observed using confocal microscopy; the alkaline comet assay was used for assessment repairing activity of UV-induced DNA damage. The in vivo research has been conducted in a group of 30 volunteers. We have measured skin moisturisation, sebum secretion, elasticity, transepidermal water loss and micro topography of the forearm and skin face. The folic acid, depending on concentration, improved viability of the primary human fibroblasts and stimulates its proliferation. The folacin-treated cells in contrast to the control cells were very regular in shape (spindle-shaped) with high ability of reproduction. Interestingly, we have found that treatment with folic acid increased the rate of repair of UV-induced DNA damage. Our data suggest that folic acid modulates DNA repair and the observed effects apparently are due to accelerated rejoining of strand breaks. In vivo tests showed that 30-day treatment with cream containing folacin improved the skin moisturisation, decreased TEWL without any significant change of sebum secretion. Skin elasticity was almost two times greater after using tested cream and analysis micro topography showed decrease of skin roughness, number of trough and irregularity and desquamation index.