Melastoma malabathricum, commonly known as senduduk, is a member of the Melastomataceae family. It is well-known for its therapeutic and medicinal properties, deeply rooted in local traditional knowledge and supported by scientific evidence. Often considered a robust plant and even labeled as a weed, M. malabathricum can be easily spotted along roadsides. However, despite its potential value, recent publications have provided limited information about its leaf anatomy. This study aimed to improve species identification by thoroughly examining the leaf anatomical characteristics of M. malabathricum. Leaf samples were collected from 10 different locations in Peninsular Malaysia and subjected to laboratory procedures involving sectioning and staining. The prepared permanent slides were then observed under a light microscope. A total of 35 leaf anatomical characteristics, including 10 types of trichomes, were successfully identified. Important characteristics such as the petiole, midrib, marginal outline, paracytic stomata, and vascular bundle types were systematically documented. Notable discoveries included the presence of hypodermal layers, collenchyma cells, druses, mucilage cells, and medullary phloem, all of which are distinctive anatomical traits of M. malabathricum. During the leaf anatomical analysis, it became evident that the majority of examined M. malabathricum samples displayed substantial similarities with minor variations in the combination of trichome types on the leaf surface. These variations might be influenced by ecological and geological factors. Nonetheless, it is essential to emphasize that the types of trichomes and other anatomical characteristics remained consistent within each sample. Consequently, these findings offer valuable insights into the systematic classification of M. malabathricum through the documentation of leaf anatomy characters. ABSTRAK Melastoma malabathricum, biasanya dikenali sebagai senduduk, merupakan famili Melastomataceae. Ia terkenal dengan sifat terapeutik dan perubatannya, secara mendalam berteraskan pengetahuan tradisional tempatan dan disokong oleh bukti saintifik. Melastoma malabathricum sering ditemui sepanjang tepi jalan dan tergolong dalam kategori rumpai yang tersebar dengan luas. Walaupun tumbuhan ini mempunyai potensi dalam perubatan, namun penerbitan berkaitan maklumat anatomi daunnya adalah terhad. Kajian ini bertujuan untuk menambah baik pengecaman spesies dengan memeriksa dengan teliti ciri-ciri anatomi daun M. malabathricum. Sampel daun telah dikumpulkan dari 10 lokasi berbeza di Semenanjung Malaysia dan seterusnya terlibat dengan kerja pemotongan dan pewarnaan di makmal. Kemudian penyediaan slaid kekal dijalankan untuk tujuan pemerhatian di bawah mikroskop cahaya. Sejumlah 35 ciri anatomi daun, termasuk 10 jenis trikom telah berjaya dikenalpasti. Ciri-ciri penting seperti bentuk luaran petiol dan midrib, tepi daun, stomata jenis parasitik, dan jenis berkas vaskular secara sistematik didokumenkan. Penemuan ketara termasuk kehadiran lapisan hipodermal, kolenkima sel, drus, sel musilaj, dan floem medulari, telah dikenalpasti sebagai ciri tersendiri M. malabathricum. Kesemua sampel M. malabathricum yang dikaji menunjukkan persamaan yang ketara dengan variasi kecil dalam gabungan jenis trikom pada permukaan daun. Variasi ini mungkin dipengaruhi oleh faktor ekologi dan geologi. Walau bagaimanapun, jenis trikom dan ciri anatomi lain kekal konsisten dalam setiap sampel. Justeru, kajian ini dapat membuktikan bahawa dokumentasi ciri anatomi daun dapat membantu pengelasan sistematik M. malabathricum.