Velmurugan Vivek. et al. / JPBMS, 2010, 1 (01)
1 Journal of Pharmaceutical and Biomedical Sciences (JPBMS), Vol. 01, Issue 01
Available online at www.jpbms.info
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL SIENCES
SHILAJIT IN MANAGEMENT OF IRON DEFICIENCY ANAEMIA
, D.Sheshadri Shekar
, SP. Sudha
& T. Sundaram
Department of Pharmacology, Sri K.V College of Pharmacy, M.G.Road, Chickballapur. (KA)-562101
Department of Pharmaceutical chemistry, Sri K.V College of Pharmacy, M.G.Road, Chickballapur. (KA)-562101
Department of Pharmaceutics, Sri K.V College of Pharmacy, M.G.Road, Chickballapur. (KA)-562101
The Shilajit as a dietary supplement was examined for iron deficiency anaemia in experimental animals. In this study diet
induced and bleeding technique were used to evaluate the iron deficiency anaemia. In diet induced, low iron diet from
sigma and in bleeding technique bled 2ml of blood from each animal for 5 alternative days was used to induce iron
deficiency anaemia (IDA), the animals which did not developed anaemia i.e. haemoglobin level < 9g/dl, were rejected and
replaced with new animals. The Shilajit 500 mg/kg shows significant (p<0.01) increase in Hb, RBC & PCV values in both
the model. According to our results Shilajit in doses of 500 mg/kg reveals anti-anaemic activity.
Key words: Shilajit, iron deficiency anaemia, haemoglobin, bleeding technique & low iron diet.
Anaemia is a decrease in number of red blood cells (RBCs)
or less than the normal quantity of hemoglobin in the
blood. Anemia is estimated to affect nearly two thirds of
the pregnant women in developing countries.Iron
deficiency anemia is responsible for 95% of the anemias
during pregnancy[2-3], [(Breymann C) (Yaqoob N), 2002].
In underdeveloped countries, anemia is a major
contributory factor to maternal morbidity and
mortality.Shilajit, a traditional medicine has been used
for cardioprotective, anti-asthmatic, anti-diabetic,
hepatoprotective, anaemic and potent CNS activity for
ages.In the Charak Samhita, Shilajit is described as a
product of four minerals: gold, silver, copper and iron,
whereas Susruta Samhita included two more minerals,
lead and zinc in its composition.The present study has
been designed to evaluate the anti anaemic activity of
shilajit in bleeding and diet induced IDA model in rats. The
drug has been able to raise the Hb.
when used in severely anemic iron deficient in rats.
Materials and Methods
Shilajit Dose Determination
The dose of shilajit was determined by acute toxicity study.
As per OECD 425 guidelines the acute toxicity study was
studied and it was found that the LD
is 5000 mg/kg body
weight. The effective dose calculated from one by tenth of the
The animal experimental protocol was approved by
Department of Pharmacology,Sri K.V College of Pharmacy,
Institutional Animal Ethical Committee as per the guidance of
the Committee for the Purpose of Control and Supervision of
Experiments on Animals (CPCSEA) 117/99/kvcp.
Iron deficiency model
Bleeding Induced IDA
This test was followed by the method described by
McCance, R. A., 1937 with some modifications. In the
model a total of 18 rats were divided into three groups of
six each. The Group II &III rats were rendered Anemia by
removing 2ml of blood from retro orbital plexus of each
rat for 5 alternative days. Group III rats were treated with
Shilajit 500mg/kg/day from the11th day after conforming
anemia to 20
day from the initial bleeding. The blood
samples were collected on 21
day for estimating the
Low Iron Diet Induced IDA
Three groups of six rats each were used for study. Group I
was maintained on normal rat diet, whereas Group II & III
were maintained on an iron deficient diet.[7-8], for 13
days before study. The diet was served in porcelain dishes.
The rats were fasted 1 day before study and Shilajit was
administered to Group III rats, 14 days after beginning the
iron-deficient diet upto 20
day. The blood samples were
collected at the end of the 20
day for estimating the
Bleeding induced IDA
Shilajit significantly increased the level of Hb, HCT and
RBC against the anaemia induced by bleeding. The values
are shown in the Table 1
Low iron diet induced IDA
Velmurugan Vivek. et al. / JPBMS, 2010, 1 (01)
2 Journal of Pharmaceutical and Biomedical Sciences (JPBMS), Vol. 01, Issue 01
Results (Table 2) reveled that Shilajit shows the significant (p < 0.01) anti-anaemic activity at dose of 500mg/kg.
Table 1: Effect of Shilajit on bleeding induced IDA Table 2 : Effect of Shilajit on low iron diet induced IDA
Groups Hb g/dl HCT % RBC
8.2 ± 0.2460
Values are in mean ±SEM; (n=6), ** p<0.01 vs. induced Values are in mean ±SEM; (n=6), ** p<0.01 vs. induced
The Shilajit’s increased the Hb, HCT and RBC when
assessed using the bleeding model and in low iron diet
induced model. About 1 mg of iron is lost each day through
sloughing of cells from skin and mucosal surfaces,
including the lining of the gastrointestinal tract.
Menstruation increases the average daily iron loss to
about 2 mg per day in premenopausal female adults.
The iron depletion leads to Hb deficiency. Hemoglobin
(found inside RBCs) normally carries oxygen from the
lungs to the tissues, anemia leads to hypoxia (lack of
oxygen) in organs, because all human cells depend
on oxygen for survival. Shilajit containing iron, when taken
as a dietary supplement it increase the haemoglobin level
and it neutralizes the regular loss and during
menstruation. Hence the present study shows that Shilajit
exhibited activities in various degrees against both models
In conclusion, this study has shown that the Shilajit having
significant anti-anaemic activity in both model. Our data
obtained from the present study suggest that the Shilajit
can be taken as a dietary supplement in the management
of iron deficiency anaemia.
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7.90 ± 0.12