Sarcostemma acidum is widely used by the folk people in the treatment of verity of diseases and disorders. The present study was therefore carried out to provide requisite pharmacognostic details about the plant. Methods: macroscopy, microscopy, physicochemical analysis, preliminary phytochemical screening and other WHO recommended parameters for standardization were performed. Result: A perennial leafless, jointed shrub with green, cylindrical, fleshy glabrous, twining branches having milky white latex, taste bitter; Flower white or pale greenish white with fragrant odour. The microscopy reveals dicotyledonous plant having circular vascular bundles in stem, absence of pith in root and anomocytic type of stomata on stem bark. Other physicochemical tests like moisture content, ash value, extractive values were determined and Preliminary phytochemical screening showed presences of carbohydrates, triterpenoids, phytosterols, saponins and lignins. Extraction of chemical constituents and chromatographically analysis (TLC) were performed. Conclusions: The microscopic using histological identification, microscopic constants, and other physico chemical examinations of Sarcostemma acidum can be used as a rapid, inexpensive and botanical identification technique is useful in standardization, hence would be of immense value in authentication of plan. © 2014, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.