Aim: To study the effects of pine pollen on the intervention in rats with hyperplasia of prostate. Methods: The experiment was conducted at Room of Microelement, General Hospital of Chinese PLA from July to September 2003. Twenty-four clean-grade SD male rats aged 3 months were selected, and divided randomly into normal control group, starch placebo group and pine pollen group with 8 rats in each group. The rats in the normal control group were fed with common feed all the time. Those in the starch placebo group and pine pollen group were treated with 3 placebo starch tablets and 3 pine pollen tablets, respectively every day before giving feed. The total content of tablet and feed was the same to that in the normal control group. After 2-week adaptation, those in the normal control group were injected with 1 mL/kg olive oil every day into muscle. Those in the starch placebo group and the pine pollen group were injected with 4 mg/kg androtest parenteral solution dispensed by olive oil into muscles every day for 2 weeks, and the same feed way was kept. Four weeks later they were killed after anesthesia, and the examination on every indexes was performed. Serum homosteron and destradiol were detected with chemiluminescence. Blood biochemical index was measured with atom absorption spectrophotography. Superoxide dismutase (SOD) in erythrocyte and tissue was detected with xanthine oxidase method. Malondialdehyde (MDA) in serum and tissue was tested with thiobarbituric acid (TBA) colorimetric method. Results: Totally 24 included rats in th e experiment were involved in the result analysis. 1 Prostate index and observation result of pathological section: The prostate weight and index in the pine pollen group were lower than those in the starch placebo group [(1 062.3±91.9), (1 127.3±111.2) mg; (3.18±0.31), (3.26±0.31) mg/g; P < 0.05]. The pathological section showed that in the starch placebo group the prostate gland arranged very close; the epithelial cells appeared stratified shape or sham stratified shape. The papillary hyperplasia ratio was much higher than that in the pine pollen group with ectasia and hyperemia of small vessels of mesenchymal. While the gland volume in the pine pollen group shortened significantly. The epithelial cells showed simple layer cube or thin and flat shape, and the papillary hyperplasia decreased significantly. 2 Biochemical index: Compared with the normal control group, the glucose, total cholesterol, triacylglycerol, urea nitrogen and destradiol in the pine pollen group reduced significantly [(6.43±2.04), (4.07±1.38) mmol/L; (1.78±0.10), (1.49±0.17) mmol/L; (1.11±0.30), (0.61±0.10) mmol/L; (9.42±0.97), (7.44±0.79) mmol/L; (253.23±40.55), (199.12±37.09) nmol/L; P < 0.05]. Compared with the starch placebo group, the homosteron content in the pine pollen group fell down significantly [(11.85 ±8.25), (8.85 ±2.69)nmol/L, P < 0.05]. 3 Zinc and copper in serum and tissue: Compared with the normal control group, the contents of zinc and copper in plasm decreased significantly. [(1.60±0.20), (1.34±0.16); (1.49±0.16), (1.17±0.05)mg/L; P < 0.05]. The contents of zinc and copper in prostate increased significantly [(130.2±29.9), (187.9±56.0); (0.86±0.12), (1.07 ±0.39)mg/L; P < 0.05]. 4 Anti-oxidizing index: Compared with the normal control group, the activities of SOD in the liver in pine pollen group and prostate were all increased significantly [(3.18±0.57), (3.30 ±0.64); (2.90±1.41), (4.67±1.66) μkat/g; P < 0.05], while the activities of SOD in erythrocyte lightened obviously [(0.38±0.05), (0.30±0.04) μkat/g; P < 0.05]. The content of MDA in the liver and prostate decreased remarkably [(7.40±1.87), (5.85±1.20); (6.24±2.31), (5.67±2.30) nmol/g; P < 0.05]. Conclusion: The pine pollen not only can decrease the levels of body estrin and androgen, adjust microelement metabolism and activity of SOD, inhibit the hyperplasia of prostate, but its effective component can play selective inhibition on cell level through non-hormone way.