Article

Evaluation of anti-diarrhoeal activity of fenugreek seed extracts in rats

Authors:
  • BLDEA's COLLEGE OF PHARMACY,BIJAPUR-586103,KARNATAKA
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Abstract

Fenugreek (Trigonella foenum-graecum) seeds are used as spice, vegetable and medicinal plant. It is therefore important and useful to identify plants with anti-diarrhoeal activity. Fenugreek seeds are quoted by many traditional healers as a plant with this activity. Therefore, the present study was undertaken to evaluate the effect of methanol and aqueous extracts of T. foenum-graecum for its anti-diarrhoeal activity against several experimental models of diarrhoea in Albino Wistar rats. The anti-diarrhoeal activity of methanol and aqueous extracts of was evaluated using castor oil-induced diarrhoea model in rats. Further, we screened the effect of methanol and aqueous extracts on gastrointestinal tract motility after charcoal meal administration and PGE 2 induced intestinal fluid accumulation (enteropooling). The plant extracts showed significant (P<0.01) inhibitor activity against castor oil induced diarrhoea and PGE 2 induced enteropooling in rats when tested at 250 mg/kg, 1ml/rat. The methanolic extract showed P<0.01 and aqueous extract (P<0.05) reduction in gastrointestinal motility in charcoal meal test in rats. Our observations suggest that methanolic extract of TFG seed has significant anti-diarrhoeal activity compared to aqueous extract.

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... Mullaicharam et al., (2013) [25] reported that the aqueous extract and a gel fraction isolated from the fenugreek seeds have cytoprotective effect due its antisecretory action. Virupaksha et al., (2007) [57] reported that in rats at the dose rate of 250mg/Kg fenugreek plant extracts showed significant (p≤0.01) inhibitory activity against PGE2 induced enteropooling and castor oil induced diarrhoea. ...
... Mullaicharam et al., (2013) [25] reported that the aqueous extract and a gel fraction isolated from the fenugreek seeds have cytoprotective effect due its antisecretory action. Virupaksha et al., (2007) [57] reported that in rats at the dose rate of 250mg/Kg fenugreek plant extracts showed significant (p≤0.01) inhibitory activity against PGE2 induced enteropooling and castor oil induced diarrhoea. ...
... The antidiarrheal effect of Solanum rudepannum was not reported in the literature; however the antidiarrheal effects of other ingredients of sundai vatral chooranam were reported (Mandal et al., 2010;Mehmood et al., 2011;Sairam et al., 2003;Virupaksha et al., 2007). Our previous studies in this area also documented the usage of Pongamia pinnata for cuts and wounds (Pandikumar et al., 2011;Mutheeswaran et al., 2011); the wound healing effect of a formulation containing Pongamia pinnata was reported earlier (Shailajan et al., 2011). ...
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