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Copernican Letters
®
Vol 1/2010
ISSN: 2082 968X
75
The use of synthetic polypeptides in cosmetics
(INDEX: 74-81/2010 Copernican Letters
®
Vol 1)
Beata Łubkowska
1
, Beata Grobelna
2
, Zbigniew Maćkiewicz
1
1
Department of Polypeptides Chemistry,
2
Department of Analytical Chemistry,
Faculty of Chemistry, University of Gdańsk, ul. Sobieskiego 18/19, 80-952 Gdańsk, Poland
b.lubkowska@gmail.com
Abstract
Cosmetic peptides are one of the active
components of modern day cosmetic preparation. Peptides
are short chains sequences of amino acids. Amino acids are
the basic building blocks of proteins and many other
different types of organic molecules. Many skincare
products use peptides to treat wrinkles. There are three main
groups of anti wrinkle peptides: signal peptides,
neurotransmitter–affecting peptides and carrier peptides.
This article reviews the most popular peptides used in
cosmetics. According to them established own sequences of
peptides which were synthesized and used in the subsequent
studies.
1. Introduction
On the cosmetics market appeared
peptides, which have to replace the popular
hydrates and hydrolysed collagen and
elastin. Peptides are transmitters of
information in the tissues, which makes it a
great demand for this type of ingredients
used in cosmetology and pharmacy.
Peptides in cosmetics are usually called
biomimetic peptides, which means
substances that mimic the mechanism of
action and the effects exerted by their
natural counterparts. Introduction to
cosmetic products of peptides is the result of
research conducted in–vitro and in–vivo,
confirming the therapeutic action of these
substances in many biological processes.
2. Bacground
As far back as the 1930s, yeast extracts
have been used in medications for their
enhancement of wound healing [1]. As
technology progressed, this allowed a
protein fraction to be recovered from the
extract of fermented yeast. Through studies
of this protein extracts, a beneficial effect on
wound healing and improved collagen
synthesis has been demonstrated [2, 3].
To date, more than 500 assorted proteins
have been identified from the yeast
Sachcaromyces cerevisiae [4]. Through these
research it was noted that peptides, which
have low molar mass, are the growth of the
yeast enzyme factories. The peptides are
theorized to up–regulate cellular growth
factors leading to skin healing as a result of
the simulation of angiogenesis and
granulation tissue and new collagen
synthesis [5]. From this early events,
peptides began to existing as ingredients in
cosmetics.
3. Peptides in cosmetics
Peptides as components of cosmetics are
small particles consisting of 6–7 amino acids,
but there are also exceptions to this rule (8–
and 20– peptides have been found). Peptides
are characterized by small molecular
weights (500–1000 Da). Short molecules
more easily penetrate the epidermis. Long
linear molecules, more or less branched,
more difficult to penetrate the cells of the
stratum corneum. Peptides, depending on
the form of the cosmetic product can
penetrate directly through the epidermal
cells or between cells on a curvy road lipid
intercellular cement. Finally, locate in the
dermis, and the speed of this process
depends mainly on the chemical nature of
the amino acid and unnatural hydration of
the stratum corneum, for example through
dressing occlusion. At that time, hydrophilic
substances have the greatest ability to
penetrate and remain in the aquatic ground.
Lipophilic substances pene
trate and remain
in the intercellular cement.
The easiest way
to penetrate have the particles with the
amphiphilic properties.
Some peptides are able to regulate the
components produced by fibroblasts, and
after the introduction into the dermis, we
can ob
served significantly improve the
appearance of the skin profile [6].
addition, peptides have capabilities of
binding water in their structure, they are
also excellent humidifiers.
This property is
used in the preparations, which improving
hydration and
barrier function or even
relaxing mimetic wrinkles. All through
primary timbers of peptides
–
which are a component of the natural factor
dampening NMF [7]. Therefore concluded
that the peptides are active in both the
epidermis and dermis (fig. 1–
3).
Fig. 1.
Construction of human skin [8]
Fig. 2.
Peptides pass through the skin layers [8]
Fig. 3.
The effect of active peptides [8]
During studies the role of peptides in the
body and with the development of these
studies, there wa
s an opportunity to use
intellig
ingredients
reduce the
appearance
of fine lines
and wrinkles
Copernican Letters
trate and remain
The easiest way
to penetrate have the particles with the
Some peptides are able to regulate the
components produced by fibroblasts, and
after the introduction into the dermis, we
served significantly improve the
appearance of the skin profile [6].
In
addition, peptides have capabilities of
binding water in their structure, they are
This property is
used in the preparations, which improving
barrier function or even
relaxing mimetic wrinkles. All through
–
amino acids,
which are a component of the natural factor
dampening NMF [7]. Therefore concluded
that the peptides are active in both the
3).
Construction of human skin [8]
Peptides pass through the skin layers [8]
The effect of active peptides [8]
During studies the role of peptides in the
body and with the development of these
s an opportunity to use
peptides as a selectively acting biologically
active compounds. Today it is known that
the peptides are in many cases the
information relays, whose presence in the
cell determines its specific activity.
the amino acid sequen
ces, it is possible to
create a peptide selectively stimulating the
process exactly where we want.
that the mechanism of action of peptides
depending on their sequence, based on 3
core processes.
One of them is to reduce
muscle contractions, i
n effect prevents the
creation of facial wrinkles. These peptides
act on the skin as soft drugs diastolic. The
second process is the regeneration and
restoration of damaged tissue, and
subsequent stimulation of dermis cells,
responsible for the synthesis o
elastin.
Peptides are able to turn on and turn
off complex regulatory processes. In
practice, they are not miraculous elixir of
youth, which will eliminate wrinkles, but the
effect their actions will certainly be
noticeable –
the skin younge
and wrinkle clearly shallower [8].
Many research in–
vitro
and include them as components for
products to protect the skin. Dermatology
practice suggests that the results are not
always possible to translate the reactions
vivo.
Each active ingredient must be
absorbed in a stable form, capable of living
and likely to accomplish its functions in the
dermis [9]. The advantage of peptides is a
small particle size, which helps them
overcome the barriers of skin and there is no
ne
ed to close them in the media patches.
Cosmetic peptides stimulate the natural
physiological processes in the skin. They are
active in low concentrations and meet the
global requirements of components for
cosmetics, such as excellent chemical and
microbiol
ogical purity and guaranteed
stability [10]. Cosmeceutical peptides can
cause real benefit to their patients, if they
know how and when they should apply
them.
Confirmed the clinical benefit is
improved skin hydration and consequently
improve the appearanc
e. This suggests that
dermis
epidermis
subcutenous
Intelligent
ingredients
enter the skin
intellig
ent
ingredients
reduce the
appearance
of fine lines
and wrinkles
Copernican Letters
®
Vol 1/2010
ISSN: 2082 968X
76
peptides as a selectively acting biologically
active compounds. Today it is known that
the peptides are in many cases the
information relays, whose presence in the
cell determines its specific activity.
Knowing
ces, it is possible to
create a peptide selectively stimulating the
process exactly where we want.
It was found
that the mechanism of action of peptides
depending on their sequence, based on 3
One of them is to reduce
n effect prevents the
creation of facial wrinkles. These peptides
act on the skin as soft drugs diastolic. The
second process is the regeneration and
restoration of damaged tissue, and
subsequent stimulation of dermis cells,
responsible for the synthesis o
f collagen and
Peptides are able to turn on and turn
off complex regulatory processes. In
practice, they are not miraculous elixir of
youth, which will eliminate wrinkles, but the
effect their actions will certainly be
the skin younge
r, more elastic
and wrinkle clearly shallower [8].
vitro
using peptides
and include them as components for
products to protect the skin. Dermatology
practice suggests that the results are not
always possible to translate the reactions
in–
Each active ingredient must be
absorbed in a stable form, capable of living
and likely to accomplish its functions in the
dermis [9]. The advantage of peptides is a
small particle size, which helps them
overcome the barriers of skin and there is no
ed to close them in the media patches.
Cosmetic peptides stimulate the natural
physiological processes in the skin. They are
active in low concentrations and meet the
global requirements of components for
cosmetics, such as excellent chemical and
ogical purity and guaranteed
stability [10]. Cosmeceutical peptides can
cause real benefit to their patients, if they
know how and when they should apply
Confirmed the clinical benefit is
improved skin hydration and consequently
e. This suggests that
Copernican Letters
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Vol 1/2010
ISSN: 2082 968X
77
the cosmetic industry got a tool that with
surgical precision affects the condition of
our skin. Looking optimistically to the future
with the amino acids can make a lot of
combinations with anti–wrinkle properties
[9].
3.1. Signal peptides
Skin is made up mostly of collagen
which is the foundation that gives skin its
support and thickness. Young people have
lots of collagen and taut, smooth skin. In
contrast, older people have much less
collagen and thin, wrinkled skin. Collagen is
protein and is made up long chains of amino
acids strung together. When it is broken
down, short segments of 3–5 amino acids
form, called peptides. Peptides are not just
junk collagen, but these “mini proteins” are
active molecules using as ingredients in lots
of creams and lotions designed to improve
the appearance of aging skin.
Signal peptides with the ability to
increase fibroblast production of collagen
can decrease collagenase breakdown of
existing collagen [11]. When we age collagen
is destroyed but not replaced. As a result
young, smooth skin becomes thin and
wrinkled over time.
Signal peptides are stimulating peptides,
which are applied to the reconstruction of
elastic layers of the skin. In fact, their
mechanism of action is based on the cellular
functions of fibroblasts. Over time,
fibroblast activity decreases, the
consequence is a slowing down of protein
synthesis which support for the skin. This is
one of the factors associated with biological
aging, which leads to the formation of
wrinkles and loss of firmness. Introduced
from outside the molecule peptides of
appropriate sequence of amino acids are
able to stimulate fibroblasts. Stimulate the
production of skin proteins that are
degraded with age. There are also a signal
for fibroblasts to increased production of
proteins, according to their name. Stimulate
also peptidoglycans, fibronectin,
glycosaminoglycans [8].
As mentioned earlier biologically active
peptides are small molecules, consisting of
few amino acids. However, because of their
specific structure, the cuticle is a barrier for
them not to move. This problem was
resolved by connecting the peptide chain
fatty acids, whose presence in the structure
allows a good penetration through the skin
and easier access to the destination [12]. The
process of obtaining such peptides IQ rely
on cut the chains of amino acids which have
sufficient sequence from a much larger
protein molecules. Then join them to the
chain fatty acids. This occurs in the process
lipofilization of peptides (fig. 4).
Fig. 4. Lipofilization of peptide [12]
Through this process, peptides can penetrate
the precision of skin cells, which stimulate
the production of new collagen by activating
the appropriate genes. This allows you to
rebuild the destroyed structures of the skin
and smooth wrinkles. Whereas the process
of regeneration of damaged fibers in the
skin. In addition, collagen causes marked
increase in skin firmness and elasticity, and
halting the processes of its flaccidity, and
biological aging [12].
The research of in–vitro and in–vivo
demonstrated a beneficial effect
cosmeceutical peptides, both on the skin
covered by chronological aging and the skin
affected photoageing. Reducing degradation
of collagen fibers, improving the
compactness of the skin, improve skin
appearance of the profile, and faster wound
modified
peptides
palmitic acid peptide
lipofilization
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healing effects are confirmed in the in–vitro
research. By contrast, research conducted in
living organisms show shallow and reduce
the length of wrinkles and improve
hydration of tissues, as well as smoothing
the skin. Signal peptides found in cosmetics
are fighting the aging of the skin.
Nowadays there are several known
bioactive amino acid sequences which are
commonly included in cosmetic formulas.
The most popular signal peptide for
cosmetic use is the sequence lysine–
threonine–threonine–lysine–serine (KTTKS)
found on type I procollagen. It is currently
marketed under the name of palmitoyl
pentapeptide–3 or Matrixyl
®
(Sederma) [13].
This pentapeptide has been demonstrated to
stimulate feedback regulation of new
collagen synthesis and to result in an
increased production of extracellular matrix
proteins such as types I and II collagen and
fibronectin [14]. KTTKS is linked to palmitic
acid in order to enhance delivery through
the epidermis for action in the dermis. It is
also considered to be a synthetic isomer (an
isomer is a molecule that has an identical
structure to another molecule but different
atomic components). Matrixyl
®
stimulates
the lower layers of the skin to heal
themselves, thus accelerating the healing of
wounds. Cells called fibroblasts are
responsible for knitting together wounds of
the skin. As you may know, as one ages
wounds take longer to heal. This is partially
because fibroblasts slowly lose the capacity
for collagen production. Matrixyl
®
stimulates the "matrix" layers in the skin –
primarily collagen and fibronectin. When
stimulated, the skin produces more collagen.
Loss of collagen is what leads to thinning
skin and the wrinkling of newly inelastic
skin. Matrixyl helps to counteract this
natural part of the aging process.
Another signal peptide is tripeptide,
lysyl–valine–lysine, has been shown to have
similar effects on the up–regulation of
transforming growth factor–beta when
combined with palmitic acid and
bistrifluoroacetic acid. Ths peptide is
currently marketed under the name of
palmitoyl–tripeptide–3/5 or Syn
®
–Coll [15].
Applying signal peptides directly to skin
is a way to replace lost collagen and treat
aging skin. When collagen breaks down, it
forms specific peptides. These peptides act
as a signal to tell your skin it was damaged.
Introduced from the outside active molecule
of peptides stimulate skin cells and make
new collagen. As a result, loss of collagen is
reconstructed.
3.2. Neurotransmitters–affecting peptides
Neurotransmitters–affecting peptides are
compounds called relaxant peptides due to
the function they perform. Their specific
activity rely on the relaxation muscles
responsible for the formation of the mimic
wrinkles. Peptides of this group work by
blocking the flow of nerve impulses from
cells located in the skin to the muscles.
Therefore, compounds acting by this
mechanism, referred to as neuropeptides.
Their activity is similar to the mechanism of
botulinum toxin injection. The effect of this
treatment is almost immediate smoothing
wrinkles. Action of neuropeptides is not as
strong as the effect of botulinum toxin.
However, regular use of products containing
this compounds give excellent results. In
addition, the advantage of therapy
neuropeptides is that they are not invasive
and do not cause complications. These
peptides have a beneficial effect on the state
of the skin: moisturize it, liquor, firm and
reduce the roughness. These features make
the preparations of peptides can also be used
after treatments with botulinum toxin for the
fixation of their performance [12].
The idea of applications neuropeptides
in cosmetic was due to previous use of
botulinum neurotoxin (Botox) in aesthetic
medicine to reduce facial wrinkles. Scientists
have long worked on the syntheses of this
compound counterpart, indicating a much
lower toxicity and similar therapeutic
effects. Studies on peptides helped to create
oligopeptides with similar mechanism of the
action which has botulinum toxin. Peptides,
which slow down transmission of ne
impulses
function as cosmeceuticals
minimize facial muscle contractions,
resulting in the reduction of wrinkles.
of them occur on the cosmetics market in the
name of patented, because of the therapeutic
effect [11].
Acetyl hexapeptide–
3 (Ac
Met–Gln–Arg–Arg–NH
2
) is a synthetic
peptide patterned from the N
–
of the protein SNAP–
25 that inhibits SNARE
complex formation and catecholamine
release. This peptide is currently marketed
as Argireline
®
(McEit [Tianjin] International
TradeCo
., Ltd.) [16]. Acetyl hexapeptide
was found to inhibit vesicle docking by
preventing formation of the ternary SNARE
complex, which is involved in synaptic
vesicle exocytosis [17, 18].
The neuropeptide
reduces the intensity of muscle tension,
which promot
es ease and reduce facial
wrinkles, induced by continuous facial
movement.
Argireline
®
is the active and safe
ingredient, non–
toxic, topical alternative to
Botox (Botulinum toxin type A), and has
been scientifically proven effective in
reducing wrinkles an
d preventing the
formation of new lines in a safe and natural
manner. The discovery of this hexapeptide
(six naturally occuring amino acids in
combination) was an exciting find, as
Argireline
®
has proven to inhibit two
internal processes that strongly cont
the formation of wrinkles. The first is: the
SNARE complex, a natural formation of
proteins in aging skin that stimulates
neurotransmitters, provoking facial muscle
contractions, thereby causing wrinkles and
lines. The second is: overproduction a
release of catecholamine–
hormones, such as
adrenaline, which affect the sympathetic
nervous system and cause wrinkles through
repetitive facial movements.
Modulation of
catecholamine secretion presented below
(fig. 5–8).
Copernican Letters
which slow down transmission of ne
rve
function as cosmeceuticals
minimize facial muscle contractions,
resulting in the reduction of wrinkles.
Most
of them occur on the cosmetics market in the
name of patented, because of the therapeutic
3 (Ac
–Gly–Glu–
) is a synthetic
–
terminal end
25 that inhibits SNARE
complex formation and catecholamine
release. This peptide is currently marketed
(McEit [Tianjin] International
., Ltd.) [16]. Acetyl hexapeptide
–3
was found to inhibit vesicle docking by
preventing formation of the ternary SNARE
complex, which is involved in synaptic
The neuropeptide
reduces the intensity of muscle tension,
es ease and reduce facial
wrinkles, induced by continuous facial
is the active and safe
toxic, topical alternative to
Botox (Botulinum toxin type A), and has
been scientifically proven effective in
d preventing the
formation of new lines in a safe and natural
manner. The discovery of this hexapeptide
(six naturally occuring amino acids in
combination) was an exciting find, as
has proven to inhibit two
internal processes that strongly cont
ribute to
the formation of wrinkles. The first is: the
SNARE complex, a natural formation of
proteins in aging skin that stimulates
neurotransmitters, provoking facial muscle
contractions, thereby causing wrinkles and
lines. The second is: overproduction a
nd
hormones, such as
adrenaline, which affect the sympathetic
nervous system and cause wrinkles through
Modulation of
catecholamine secretion presented below
Fig. 5. Normal synapse [16]
Fig. 6.
Partial secretion blocking [16]
Fig. 7. Synaptic distance increase [16]
vesicles
containing
catecholamine
receptor
receiving
vesicles
containing
catecholamine
receptor
receiving
vesicles
containing
catecholamine
receptor receiving
cell
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ISSN: 2082 968X
79
Partial secretion blocking [16]
Fig. 7. Synaptic distance increase [16]
synapse
catecholamines
receiving
cell
synapse
catecholamines
receiving
cell
catecholamines
cell
synapse
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Fig. 8. Reversible in 6 months [16]
Argireline
®
works by inhibiting both the
SNARE complex and overproduction of
catecholamine, skin smoothes out. Also
reduces the intensity and frequency of
neurotransmitters that cause muscle
contractions.
3.3. Carrier peptides
Carrier peptides are a small family of
peptides that include a copper molecule in
their structure. These peptides have been
studied due to their ability to encourage the
skin to heal wounds. Indeed, carrier
peptides certainly have a salutary effect on
various tissue types: the stomach lining,
bones, and intestines as well as skin.
Peptides are small fragments of proteins, but
proteins are the key building blocks of
nearly all living tissues. Certain kinds of
peptides have an avid affinity for copper
atoms, to which they bind themselves very
tightly. The resulting compound consisting
of a peptide and a copper atom are known
as copper peptides or carrier peptides.
In the 1970s, researchers discovered the
healing properties of copper peptides. Put
simply, copper peptides seem to encourage
the body's own healing mechanisms. Dr.
Loren Pickart discovered that a variety of
copper peptides have the ability to aid in
tissue regeneration. Copper peptides
boosted the body's healing abilities. Dr.
Pickart patented a number of specific copper
peptides. They seemed to be particularly
effective in healing wounds, skin lesions and
even gastrointestinal ulcers. In addition,
copper peptides help wounds heal. Copper
peptides break down a specific type of tissue
that forms scars (known as extra–large
collagen aggregates). When these aggregates
are broken down, wounds heal with far less
scar tissue. Copper peptides also offer a
significant anti-inflammatory benefit. In
short, they help wounds heal faster, better
and with less scarring [19].
A popular carrier peptide is tripeptyd
Gly–His–Lys, which forms a complex with
copper ions and has an important function
in our skin. This peptide sequence is
consistent with that found in the
extracellular matrix proteins as the α-chain
fragment of collagen and is released from
collagen during wound healing or
inflammation. Peptide GHK shows a wide
range of biological activities such as
stimulation of hair growth, collagen syntesis,
superoxide dismutase – like activity, wound
healing, tissue repair and angiogenesis [19,
20]. Tripeptide also possesses a high affinity
for Cu
2+
ions and spontaneously builds a
complex (GHK–Cu). GHK–Cu also plays a
key role in mammalian organism because it
directly participates in wound healing and
tissue repair [20–22].
For cosmetic purposes, GHK–Cu
complex was created synthetically, starting
from the isolation from human plasma.
Copper peptide was developed to improve
the transfer capability of copper in the
tissues, increase flexibility and strengthen
the skin of fibers. This combination of metal
and peptide enhances the level of
MMP/TIMPs and helps restore the youthful
appearance of skin tissue. Additionally, it
causes the synthesis of type I collagen in
fibroblasts and synthesis dermatan sulfate,
heparin sulfate, cytochrome–C oxidase and
tyrosinase. Dermatan sulfate and heparin
sulfate are substances from the group of
glycosaminoglycans, or mucopolysaccha-
rides hygroscopic properties, to ensure
constant concentration of water in the
dermis. Peptide complexes with copper
vesicles
containing
catecholamines
catecholamine
receptor receiving cell
synapse
closeness
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improves the appearance by stimulating the
regeneration of damaged skin. The complex,
on the one hand stimulates the activity of
metalloproteinases I and II (remove
damaged collagen and elastin of the
extracellular matrix), on the other hand
stimulates the synthesis of fibroblasts [23,
24].
Both tripeptide GHK and complex
GHK–Cu cause the beneficial effects by
stimulating collagen. The combination of
carrier peptide with copper increases the
level of matrix metalloproteinases MMP–1
and MMP–2 mRNA as well as TIMP–1 and
TIMP–2, which restores skin firmness [11].
The studies have shown that this modified
peptide is not only working to accelerate the
process of regeneration, but also to rebuild
damaged tissue.
Peptides containing copper can not be an
effective ingredients for wrinkles, because
wrinkles are not wounds. Wrinkles are
simply folds in the epidermis, the main
action of copper peptides is not effective on
wrinkles. Copper peptides are sometimes
used by doctors to help skin heal after
cosmetic surgical procedures or severe
chemical resurfacing. One possible
application of copper peptide is to use it
after cosmetic or dermatological treatments,
such as dermabrasion, chemical peeling, and
laser skin rejuvenation. Copper peptides can
be used to counteract some of the more
serious irritations caused by such treatment
with a derivative of vitamin A (Retin–A).
Application of peptide within two hours
after surgery to avoid local irritation,
redness and scaling associated. Copper
peptides are readily used by doctors than
vanity, it helps heal the skin after surgical or
chemical. There is not currently sufficient
research to prove that copper peptide is an
effective anti wrinkle ingredient.
4. Conclusion
Peptides in cosmetics are one of the new,
popular options to treat aging skin. Most
studies used to justify the incorporation of
these ingredients into skin care products. As
practicing dermatologists show, use of
cosmetic peptides increases from year to
year. Through in–vitro studies the following
results have been noted: improved skin
hydration, improved appearance of the skin,
increase in skin density and thickness. These
results do not always translate into in–vivo
actions. For any active ingredient to work, it
must be absorbed in the stable form into the
viable dermis. It is not an easy task to
penetrate the barrier of the skin. Therefore,
included palmitic acid helps peptides to go
through the skin barrier. There is, however,
soft clinical data and evidence that peptides
are able to overcome skin barrier and
produce beneficial effects for their patients.
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