Article

Phytological analysis for designinig a microbial consortium to enhance plant growth

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Abstract

During the past couple of decades the use of plant growth promoting rhizobacteria(PGPR) for sustainable agriculture has increased tremendously in various parts of world. Significant increases in response to inoculation with PGPR have been repeatedly reported. Biofertilizers are also available for increasing crop nutrient uptake of nitrogen from nitrogen fixing bacteria associated with roots, sulfur uptake from phosphate mineral solubilizing bacteria. In the present study consortium of microorganisms were designed to cater different needs of growing plants and to understand interaction between consortium of microbial inoculants and plant systems will pave way to harness more benefits for improving plant growth and yield. The germination percentage of the Paddy seeds control is 66.7% on fourth day but varying results was obtained with Azotobacter, Pseudomonas, Bacillus, sth1 and sth2 as 83.3%, 83.3%, 50%, 66.7% and 66.7% respectively. The vigor index value for control is 113.39. Bacillus is 40 and Azotobacter, Pseudomonas, sth1 and sth2 are 149.99, 141.6, 73.37 and 66.7 respectively. The germination index is 2 for control, sth1 and sth2 1.75 for Bacillus and 2.25 for Azotobacter and Pseudomonas. The germination percentage of green gram seeds is 100% on 4th day and germination index was calculated as 2.5 for all the organisms. The vigor index is less for control (160) and 210 for Bacillus and sth1, 200for sth2, 180 for Azotobacter and 170 for Pseudomonas. The Field culture study gives a clear view of practical applications and explains the synergistic or antagonistic effect with the seeds. The results of the present study suggest that pressuring bacterial treatments have a significant effect on the plant. The field culture analysis revealed that seed inoculation with all bacteria resulted in an increased plant height and weight. Thus Azotobacter, sth1, Bacillus and Pseudomonas together as a consortium can be used as a biofertilizer for the growth of paddy, green gram and Maize. Future study can be done on the increase of seed number and yield. The designed consortium can be further formulated based on concentrations and designed for large scale production and commercialization. This when applied would increase the quality and fertility of the land.

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... Leurs métabolites secondaires présentent une source incontournable de composés divers, impliqués dans plusieurs domaines : industriel, médical et agricole (Donadio et al., 2002). Elles offrent de ce fait des résultats attrayants de manière à remplacer les engrais minéraux, les pesticides (Jelin et al., 2013) et contribuer ainsi à l'amélioration de la productivité des cultures notamment celle du maïs. Quant au chitosane, c'est un bioproduit dérivé de la chitine, un composé naturel, non toxique à l'homme, ni à l'environnement et aux animaux. ...
... En effet, les résultats montrent que l'application de P. fluorescens + chitosane + 50 % NPK a induit une augmentation de 29,01 % en hauteur des plants ( figure 3). Ces résultats se rapprochent des 12,85 % obtenus par Jelin et al. (2013) en Inde sur la hauteur. ...
... Leurs métabolites secondaires présentent une source incontournable de composés divers, impliqués dans plusieurs domaines : industriel, médical et agricole (Donadio et al., 2002). Elles offrent de ce fait des résultats attrayants de manière à remplacer les engrais minéraux, les pesticides (Jelin et al., 2013) et contribuer ainsi à l'amélioration de la productivité des cultures notamment celle du maïs. Quant au chitosane, c'est un bioproduit dérivé de la chitine, un composé naturel, non toxique à l'homme, ni à l'environnement et aux animaux. ...
... En effet, les résultats montrent que l'application de P. fluorescens + chitosane + 50 % NPK a induit une augmentation de 29,01 % en hauteur des plants ( figure 3). Ces résultats se rapprochent des 12,85 % obtenus par Jelin et al. (2013) en Inde sur la hauteur. ...
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... Plates were incubated for 24 hrs at 37˚c. After incubation the colonies developed on the flower waste agar plate were observed for different characteristics of colonies such as shape, size, elevation, surface, margin, color, odor, pigmentation etc. and gram's staining Jelin et al., 2013). ...
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