Vol. 118, n. 2 (Supplement): 57, 2013
© 2013 Firenze University Press
ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY
Platelet Rich Plasma stimulates human hair growth in
Marco Angelo Cocchi1*, Paola Bezzola2, Elisabetta Sorbellini2, Anna Trink2, Mauro Labanca1,
Veronica Bonazza1 and Fabio Rinaldi2
1 Section of Anatomy and Physiopathology, Department of Clinical and Experimental Sciences, University of
Brescia, Brescia, Italy
2 International Hair Research Foundation (IHRF), Milan, Italy
Several factors are involved in hair growth and cycling (Buoli et al., 2013).
Platelet concentrates have a new important role in regenerative medicine and thus
in dermatology, oral, plastic and orthopaedic surgery and hair growth (Franco et al.,
2012). In this study we evaluated in vitro the eects of Platelet-Rich Plasma (PRP),
an autologous platelet preparation, on hair growth. In particular, we compared four
dierent culture media (Philpott et al., 1990): 1-William’s E culture medium with
supplemented factors; 2-William’s E culture medium with supplemented factors and
Platelet Rich Plasma; 3-William’s E culture medium without supplemented factors;
4-William’s E culture medium without supplemented factors but with PRP. Hair shaft
elongation was measured at 0, 24, 48, 72 and 96 hours: digitally xed images of slices
were analyzed using an image analyzer considering as measurable portion the shaft
part between the bulb upper border and the top of the hair end. The values obtained
were used to calculate the percentage of elongation for each time. Growth in hair cul-
tured with William’s E medium with supplemented factors and PRP resulted higher
with respect to the other media. Moreover, these results suggest that PRP stimulates
human hair growth in vitro.
 Buoli et al. (2013) The human hair: from anatomy to physiology. Int J Dermatol (In press).
 Franco et al. (2012) Protocol for obtaining Platelet-Rich Plasma (PRP), Platelet-Poor Plasma (PPP),
and Thrombin for Autologous use. Aesthetic Plast Surg 36(5): 1254-1259.
 Philpott et al. (1990) Human hair growth in vitro. J Cell Sci 97(3): 463-471.
Platelet, growth factors, human hair.