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Nano silicon application improves salinity tolerance of sweet pepper plants



Salinity is a major limiting factor for crop growth and productivity especially in arid and semi arid areas. Therefore this study was conducted in order to compare the effect of applying silicon in the standard form versus the nano form on mitigating salinity negative effects on sweet pepper plants (Capsicum annumn L.) cv. California Wonder. Seedlings were transplanted in March 2013 and 2014 in North Delta, Egypt and were irrigated with saline water with an EC of 5.47 dS/m. Silicon in two forms (regular 25%) and nano silicon (25%) were supplied through irrigation systems at concentrations of 4.0 and 5.0 cm3/l for regular silicon and 1.0 and 2.0 cm3/l for nano silicon. Application took place at 3, 6 and 9 weeks after transplanting. Data showed that all plant growth aspects such as plant height, number of branches and leaves fresh and dry weights were improved under all silicon treatments compared to non treated plants. Yield parameters followed also the same trend. Among silicon treatments, concentration of 1.0 cm3/l of nano silicon recorded the highest significant effect in mitigating salinity negative effects. It could be concluded that nano silicon is more effective and efficient in mitigating salinity stress on sweet pepper plants.
Nano Silicon Application Improves Salinity Tolerance of
Sweet pepper Plants
Tantawy A.S.1*; Salama Y.A.M.2;El-Nemr M.A.1
and Abdel-Mawgoud A.M.R.1
1Vegetable Research Department, National Research Center, Dokki, Giza, Egypt
2 Plant Adaptation Unit, Genetic Resource Department, Desert Research Center, Cairo,
Abstract: Salinity is a major limiting factor for crop growth and productivity especially in arid and semi arid
areas. Therefore this study was conduct ed in order to compare the effect of applying silicon in the standard form
versus the nano form on mitigating salinity negative effects on sweet pepper plants (Capsicum annumn L.) cv.
California Wonder. Seedlings were transplanted in March 2013 and 2014 in North Delta, Egypt and wer e
irrigated with saline water with an EC of 5.47 dS/m. Silicon in two forms (regular 25%) and nano silicon (25%)
were supplied through irrigation systems at concentrations of 4.0 and 5.0 cm3/l for regular silicon and 1.0 and
2.0 cm3/l for nano silicon. Application took place at 3, 6 and 9 weeks after transplanting.
Data showed that all plant growth aspects such as plant height, number of branches and leaves fresh and
dry weights were improved under all silicon treatments compared to non treated plants. Yield parameters
followed also the same trend. Among silicon treatments, concentration of 1.0 cm3/l of nano silicon recorded the
highest significant effect in mitigating salinity negative effects. It could be concluded that nano silicon is more
effective and efficient in mitigating salinity stress on sweet pepper plants.
Key words: Sweet pepper, Salinity, Nano Silicon, Total chlorophyll, N, P, K and Total Yield.
Nowadays, salinity is became one of the most serious environmental problems that caused great
reduction ongrowth and development of plant species. In fact Salinity is one of the major yield limiting factors
for crop plants mainly in arid and semiarid regions of the world1. In Egypt the problem is aggregated due to
overuse of fertilizers and shortage of good irrigation water. Therefore, many trails and approaches have been
attempted to mitigate the well-known negative effects of salinity on plant growth and production2,3,4,5,6. Among
those approaches is the improvement of plant nutritional status via external supplements to ameliorate salinity
damages with exogenous application of K+ in wheat7, N in Phaseolus vulgaris8 and Ca in snap bean9.10reported
an improvement of tomato crop growth and production under saline conditions as a result of application of nano
calcium. Furthermore, some beneficial mineral nutrients have been studied that can counteract adverse effects
of salt stress. Silicon, being a beneficial element provides significant benefits to plants at various ionic
compositions. Also, 11proved that nono silicon application can improve seed germination and seedling growth
of tomato. Earlier, 12showed that soyabean plants supplied with silicon had higher salinity tolerance compared
to non-supplied plants. Silicon deposition in the tissues help to alleviate water stress by reducing transpiration
rate, improve light interception characteristics by keeping the leaf erect, increase resistances to diseases pests
and lodging, remediate nutrient imbalances, and there are other documented beneficial effects 13,1 4,15. Silicon
International Journal of ChemTech Research
Vol.8, No.10 pp 11-17, 2015
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presence in the cell wall fiber makes the cell wall tough and resistant to pest and pathogens attacks. Naturally,
plants contain Si in appreciable concentrations, ranging from 1% to 10% or even higher of the dry matter. This
difference of Si levels in different plant species have been attributed to the Si uptake ability of the roots16,17.
Despite of the prominence of Si as a mineral constituent of plants, Si is not considered as "essential" nutrient,
for any terrestrial higher plants except members of the Equisitaceae and is thus not included in the formulation
of any of the commonly used nutrient solutions13. As the beneficial effect of silicon has been proved as shown
above, the application of non silicon can be mor e effective than the large applied particles which means a more
efficient input use. 10showed that application of nano calcium to saline stress tomato plants ha d more efficient
and effective impact on mitigating the negative effect of salinity compared to the application of chleated
calcium. The use of the new science, nanotechnology in agriculture has begun and will continue to have a
significant effect in the main areas of breeding new crop varieties, development of new functional materials and
smart delivery systems for agrochemicals like herbicides, fertilizers and pesticides, smart systems integration
for food processing, packaging and other areas like remediation of herbicide and pesticide residues from plant
and soil, effluent water treatment, etc.18.Nano-technology can present solution to increasing the value of
agricultural products and environmental problems. Nanomaterials because of their tiny size show unique
characteristics. They can change physic–chemical properties compared totheir bulk materials, they have a great
surface area than bulk materials. Because of these larger surface areas, their solubility and surface reactivity
was higher19. By manufacturingthe preparation ways of nanomaterials can change their characteristics, for
example, the addition of nanoparticlesin liquid changes their chemical, physiological and transport
characteristics compared to their base fluids such as enhancement of thermal conductivity20.
Yet no studies were found on the effect of nano silicon application on the growth and production of
sweet pepper plants. In Egypt, sweet pepper is a major vegetable crop for local consumption and export.For
these reasons, this study was conducted to compare the effectiveness of applying silicon in the nano or regular
forms in reducing the negative effects of salinity on tomato plants growth and production.
Materials and methods:
Seeds of sweet pepper plants (Capsicum annumn L.) cv. California Wonder were sown on 15
th of
January 2013 and 2014 and seedlings were transplanted on the 15th of March in the two seasons of 2013 and
2014 in a sandy soil in a private farm in the area of Wadi El-Natron, Bahaira governorate, Egypt. The soil
physical and chemical analysis are shown in tables 1 and 2. Individual transplants were grown at the bottom of
ridges 100 cm width at 50 cm apart. Plot area was 1X12= 12 m2. The drip irrigation system of GR 16 was used
and plants were irrigated daily using saline-well water with an EC value 5.47 dS/m and pH of 7.8. The complete
chemical analysis of the irrigation water is shown in table (3).
All standard agricultural practices other than experimental treatments were applied according to the
recommendations of the ministry of agriculture, Egypt.
Experimental treatments:
After three weeks from transplanting, plants were supplied through the irrigation system with two types
of siliconforms namely Nano Silicon (Nano-Si 25%) or regular silicon (Si 25%). Each form was supplied to the
plants in two concentrations as follow: Nano-Si : 0.0 (control), 1.0 or 2.0 cm
/l. Meanwhile Si (25%) was
applied as 0.0 (control), 4.0 or 5.0 cm3/l.
Applications of silicon treatments were at 3, 6 and 9 weeks after transplanting.
Experimental design and statistical analysis:
The treatments were arranged in a complete randomized block design with three replicates and analysis
of variance was carried out at probabilit y level of 0.05. Least Significant Differ ence LSD was calculated to
differentiate between the treatments.
After 70 days from transplanting the following measurements were carried out:
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Physical measurements: Plant height, number of branches, fresh and dry weights of leaves; total yiel d
(ton/fed.); and average weight of individual fruits (g).
Chemical measurements: Total chlorophyll content (SPAD); total contents of N, P and K (%).
Table (1): Soil physical analysis and soil properties of the experimental farm.
Soil depth (cm3)Total sand (%) Silt (%) Clay (%) Texture
0-15 58.0 11.5 30.5 Sandy
30 – 60 57.0 13.0 30.0 Sandy
Table (2): Soil chemical analysis of the experimental farm.
Soluble anions (ppm) Soluble cations (ppm)
Soil depth (cm3) EC (dS/m) pH
-- Cl- SO4
-- Ca++ Mg++ Na+K+
0-30 4.77 7.7 55.85 31.20 10.50 24 11 10.52 2.18
30-60 4.16 7.4 51.21 22.50 16.10 16.83 6 17.80 0.097
Table (3): Chemical analysis of irrigation water (underground well) of the experimental farm.
Soluble anions (ppm) Soluble cations (ppm)
Water sample EC (dS/m) pH
-- Ca++ Mg++ Na+K+
Average 5.47 7.8 2.50 81.08 16.24 25.29 19.43 54.83 0.45
Data in Table (4) show that plant height was significantly reduced under salinity treatment. Meanwhile
all silicon treatments mitigated these negative effects and plant height was improved with superior effects
recorded with Nano-Si treatments which were not s ignificantly different between each ot her. Although Si
treatments significantly improved plant height under salinity conditions, they were significantly lower in their
effects compared to Nano-Si treatments. Similar trends were obser ved in the number of branches where all
silicon treatments improved this parameter compared to the untreated plants. The Nano-Si treated plants showed
the highest mitigated effects compared to Si treatments. The effectiveness of each Si treatment was clear in
leaves fresh weight (Table 4). With significant differences among all treatments, Nano-Si treatment of 1 cm3/l
showed the highest effectiveness in mitigating salinity effect on that parameter followed by Nano-Si 2.0 cm3/l
then Si 4.0 cm3/l and finally Si 5.0 cm3/l treatment. All silicon treatments showed a significant improvement in
leaves fresh weight compared to untreated plants. Dry weight of the leaves showed also a similar trend that all
silicon treatments improved that parameter compared to untreated plants grown under saline conditions.
However Nano-Si treated plants showed higher significant positive responses compared to regular silicon
supplied plants with superiority effect for 1 cm3/l treatment.
Fruit yield data are shown in Table (5). Average fruit weight of individual fruits was also improved by
all silicon treatments compared to untreated plants grown under salinity conditions. The lower concentration of
Nano-Si had the highest significant mitigating effect regarding this parameter compared to all other silicon
treatments. There was no significant difference between the highest applied concentration of Nano-Si (2 cm3/l)
and the lowest concentration of regular silicon (4 cm3/l) treatments. Also there was no significant difference
between the two concentration treatments of regular silicon. Total fruit yield (Table 5) showed also an
improvement as a result of all silicon treatments compared to untreated plants grown under saline conditions.
The Nano-Si treatment of 1.0 cm
/l concentration showed the highest significant mitigating effects on total
yield of sweet pepper plants compared to all other silicon treatments. There was no significant difference
between the two concentrations of r egular silicon treatments.
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Chemical analysis data as shown in Table (6) showed that all silicon treated plants showed an
improvement in total chlorophyll content compared to control plants grown under salinity conditions. However,
only the lowest concentration of Nano-Si treatment that was significantly higher than all other silicon
treatments. Nitrogen total content showed also an improvement as a result of all silicon treatments compared to
control plants. In addition the only significant difference among silicon treatments was between the lowest
Nano-Si treatment and the rest of s ilicon treatments. Similar trend was observed also in phosphorus total
content where all silicon treatments improved that content compared to control treatment. Among silicon
treatments, only the highest concentration of regular silicon treatment showed a significant difference compared
to other silicon treatments. Potassium total content followed the same observed trend of improvement in all
parameters as a result of silicon treatments. There was a significant differences among the treatments wit h
superiority to the lowest concentration of Nano-Si treatment.
Table (4): Effect of different silicon treatments on plant height, leaf fresh and dry weights, and number of
branches of sweet pepper plants.
2013 Season 2014 Season
Treatments Plant
No. of
/ plant
No. of
/ plant
Control 30.45 61.45 14.67 4.32 28.64 59.34 12.40 4.11
Nano Si ( 25% ) 1 cm / Lit. 46.32 98.28 26.49 7.86 44.51 96.50 23.69 7.68
Nano Si ( 25% ) 2 cm / Lit. 44.91 81.19 23.51 7.02 43.27 80.73 21.38 6.93
Si ( 25% ) 4 cm / Lit. 40.23 73.61 19.92 6.57 39.34 71.02 18.43 6.48
Si ( 25% ) 5 cm / Lit. 38.21 69.37 18.23 6.01 35.45 67.47 15.57 5.98
L.S.D. at 5 % 2.09 3.01 1.93 0.92 1.91 1.79 1.57 0.71
Table (5): Effect of different silicon treatments on individual fruit weight and total fruit yield sweet
pepper plants.
2013 Season 2014 Season
Treatments Fruit weight
Total Yield ton /
Fruit weight
(g) Total Yield ton / fed.
Control 62.23 5.32 63.11 5.11
Nano Si ( 25% ) 1 cm / Lit. 96.12 7.86 98.42 7.72
Nano Si ( 25% ) 2 cm / Lit. 85.23 7.32 82.55 7.02
Si ( 25% ) 4 cm / Lit. 79.56 6.62 78.72 6.30
Si ( 25% ) 5 cm / Lit. 74.38 6.21 70.22 6.09
L.S.D. at 5 % 5.78 0.42 5.44 0.35
Table (6): Effect of different silicon treatments on chlorophyll content and chemical composition of sweet
pepper leaves.
2013 Season 2014 Season
Treatments Total
Control 38.45 0.98 0.42 1.10 37.33 0.93 0.43 1.09
Nano Si ( 25% ) 1 cm / Lit. 49.23 1.73 0.72 2.01 47.54 1.70 0.75 1.99
Nano Si ( 25% ) 2 cm / Lit. 47.12 1.56 0.70 1.82 45.32 1.51 0.73 1.78
Si ( 25% ) 4 cm / Lit. 44.07 1.48 0.66 1.71 44.09 1.46 0.68 1.60
Si ( 25% ) 5 cm / Lit. 43.67 1.42 0.50 1.52 42.11 1.39 0.55 1.43
L.S.D. at 5 % 4.12 0.21 0.07 0.08 3.84 0.19 0.09 0.06
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It is evident that Si is beneficial for growth of many plants under various abiotic (e.g. salt, drought and
metal toxicity) and biotic (plant diseases and pests) stresses21,22. Some authors reported that Si could ameliorate
salt stress depression on plant species23,24,25,26. Indeed our results showed that the application of silicon
improved all plant growth aspects under saline conditions. 27observed that Si application leaded to balance
growth reduction of Phaseolus vulgaris L. caused by salinity like decrease stomatal conductance, drop of leaf
RWC, decrease K+ tissues contentand etc. Our results on leaf fresh and dry weights as well as potassium
contents confirm these results. Among the possible mechanisms of Si mitigation to stress is that it maintains the
plant water Status under saline conditions which resulted in higher cell expansion and leaf area compared to Si-
untreated plants grown under saline conditions27. This may explain the increment in plant height of Si treated
plants compared to untreated one under salinity conditions. 29found that plants treated with NaCl in the presence
of Si showed values of turgor potential 42% higher than those plants treated only with NaCl. This may result
also in higher stomatal conductance hence higher photosynthesis which may lead to higher dr y matter
production. In fact this may explain our result of the dry weight of the leaves. 28reported that leafturgor potential
and net photosynthesis rates were found 42 and 20% higher respectively in salt-stress ed plants treated with Si in
comparison to plants grown in Si fr ee solution. Higher chlorophyll content reported in this study contribute
further in improving photosynthesis rate and dry matter production for Si treated plants compared to control.
Improvement in water content and net photosynthesis rates must be reflected on fruit yield and this is exactly
what was found in this study with Si treated pla nts grown in saline media compared to untreated plants.
Increment in nutrient contents such as N, P and K in plant tissue is another mechanism of Si mitigation to
salinity stress. Si reduces uptake of Na+
by improving K+: Na+ and also alleviates the toxicity of other heavy
The effects of Si treatments on saline stress plants have been confirmed in our study on sweet pepper
plants. However, the nano silicon form has proved a stronger mitigating effects compared to regu lar silicon
form. Nano silicon was used earlier by31 who found that it could enhance the gr owth of soybean. They observed
that soybean seeds which treated by a mixture of Nano SiO2 and Nano TiO
had more germination and the
activity of nitrate reductase, superoxide dismutase, catalase and peroxidase of germinating seeds were increased
significantly. 10reported a more beneficial effects of nano Ca compared to chleated Ca on mitigation of salinity
stress on tomato plants. This has been also noticed in this study with the superiority of nano silicon effects
compared to regular silicon on mitigation of stress damages. This may be due to the fact that changing the
particles to nano form can change physic–chemical properties compared to their bulk materials, they have a
great surface area than bulk materials. Because of these larger surface areas, their solubility and surface
reactivity was higher20. By manufacturingthe preparation ways of nanomaterials can change their
characteristics, for example, the addition of nanoparticlesin liquid changes their chemical, physiological and
transport characteristics compared to their base fluids such as enhancement of thermal conductivity21. This may
mean mor e availability to plant absorption and higher reactivity wit hin plant tissue.
It could be concluded that application of silicon can mitigate salt stress damages on sweet pepper
plants. However, the application of nano silicon is more effective and efficient compared to regular silicon
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... Chili pepper (Capsicum annuum) Increased plant height, fresh and dry leaf weight, and fruit weight. (Tantawy et al. 2015) Si NPs ...
Horticultural crops are used for a variety of purposes, including nourishment and aesthetics. Human nutrition relies heavily on these plants for carbohydrates, proteins, organic acids, vitamins, and minerals. Drought, flood, nutrient inadequacy, heat, light, metal stress, and other abiotic stresses restrict crop plant development and output. A variety of tactics are used to improve abiotic stress tolerance, including the production of genetically modified cultivars by incorporating gene constructions to improve performance under stress. Nanotechnology is a multifaceted field that could be applied in every branch of research comprising medicine, industry and agriculture. Soil and water nanoremediation, plant nanoprotection and plant nanonutrition are some of the agricultural applications. To accomplish sustainable progress in horticultural plants challenged with various environmental challenges, numerous definitive directions have been developed to secure public acceptance and convenient utilization of nanoparticles. Even with the rapid increase in nanoparticle use in several applications, there is still a dearth of information on the actual outcomes of nanoparticle interaction with plants. This review assesses the role of nanomaterials in horticultural crops under abiotic stress, with a focus on recent research. In addition, this review focuses on explaining the broad roles of several nanoparticles in enhancing abiotic stress tolerance in horticulture plants.
... According to Almutairi (2016a), it has been found strong interactions between the enhancement of seed germination and growth in tomato-stressed plants with high salt and the increased expression of salt tolerance genes when Si NPs are applied. In contrast to no treatment of Si NPs, Capsicum annuum plants showed increased growth when irrigated with saline water upon the application of Si treatments (Tantawy et al., 2015). ...
Full-text available
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... Apart from the other abiotic stresses, nSi has been shown to enhance the growth parameters, photosynthetic pigments, epicuticular wax layer, nutrient uptake, and carbon and nitrogen metabolism of plants under salinity stress (Avestan et al., 2019;Cui et al., 2021;Yassen et al., 2017). Nano-silicon has also been observed to improve the growth and yield parameters of sweet pepper plants exposed to saline stress, more efficiently than bulk silicon (Tantawy et al., 2015). Similarly, nSi application exhibited better ameliorating effects than the bulk silicon in the case of saline-stressed tomato plants in the early growth phase (Haghighi and Pessarakli, 2013). ...
Lentil is one of the highly nutritious legumes but is highly susceptible to salinity stress. Silicon has been known to reduce the effect of various environmental stresses including salinity. Moreover, silicon when applied in its nano-form is expected to augment the beneficial attributes of silicon. However, very little is known regarding the prospect of nano-silicon (nSi) application for alleviating the effect of salinity stress in non-silicified plants like a lentil. In this study, the primary objective was to evaluate the efficacy of nSi in the alleviation of NaCl stress during germination and early vegetative stages. In this context, different concentrations of nSi (0, 1, 5, 10 g L⁻¹) was applied along with four different concentrations of NaCl (0, 100, 200, 300 mM). The results indicated the uptake of nSi which was confirmed by the better accumulation of silica in the plant tissues. Most importantly, the enhanced accumulation of silica increased the K⁺/Na⁺ ratio of the NaCl-stressed seedlings. Moreover, nSi efficiently improved germination, growth, photosynthetic pigments, and osmotic balance. On the other hand, the relatively reduced activities of antioxidative enzymes were surmounted by the higher activity of non-enzymatic antioxidants which mainly scavenged the increased ROS. Reduced ROS accumulation in return ensured better membrane integrity and reduced electrolyte leakage up on nSi application. Therefore, it can be concluded that the application of nSi (more specifically at 10 g L⁻¹) facilitated the uptake of silica and improved the K⁺/Na⁺ ratio to reclaim the growth and physiological status of NaCl-stressed seedlings.
... Silicon improved the photosynthesis rate on the sweet pepper plant and resulted in higher cell expansion and leaf area, which influence dry matter production and higher yield of sweet pepper. This is mainly because nanoscale particle was more available for plant absorption and higher reactivity within plant tissue [145]. Root colloidal silicon application significantly gives a high total tomato yield. ...
Agriculture waste has attracted attention as a potential source to produce raw material silicon dioxide, either crystalline (pyrophyllite) or amorphous form (geothermal sludge). It is an unwanted waste produced as a desired result of agricultural activities. Nanosilicon dioxide has undoubtedly gained eager interest in many vital industries. It is renowned for positively enhancing outstanding performance due to tuneable properties over its bulk counterpart. Silicon dioxide scientifically demonstrates a unique ability to convert efficiently into economic value from silicon-rich agriculture waste. Thus, a noble extraction from silicon-rich waste is undoubtedly gaining enormous attention. However, adequate knowledge on local optimisation of nanosilicon dioxide extraction from silicon-rich agriculture waste is lacking. Specific aims of this comprehensive review mainly highlighted a synthesis method of potential nanostructured silicon dioxide from agriculture waste and their potential applications for plant growth promoters. Reverse microemulsion, chemical vapour condensation, solid gelation, and mechanochemical are preferred methods that were typically specified to focus this comprehensive review critically. Optimisation of nanosilicon dioxide can be achieved precisely via the ideal combination of solid gelation and a high-energy ball mill process. Silicon dioxide is undoubtedly an effective agent as a plant growth promoter to overcome biotic and abiotic factors such as heavy metal uptake and translocation, inhibit pathogenic fungi, improve the antioxidant system, and mitigate various stress factors.
... The NUE is improved because of the high specific surface area of nano fertilizers; they earned their properties as easier absorption by the plant, which improved the efficiency and the economic benefits with some extra benefits of improving soil properties and the ability of water and fertilizer conservation (Shalaby et al., 2016;Usman et al., 2020). High crop yield and quality were reported with the application of nano-fertilizers (Tantawy et al., 2015;Prifti and Maci, 2017;Abd El-Azeim et al., 2020). A few studies have been published on nano-N, which discuss the synthesis of nano crystalline N-doped-TiO2 (Chaturvedi and Singh, 2021). ...
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Climate change has worsened the existing scenario by increasing temperature, severity of extreme droughts, elevating evapotranspiration and severe water shortage. Furthermore, excessive and unwisely application of fertilizers ultimate produce degraded agricultural land. All these consequences reduced the yield and quality of agricultural commodities to feed the increasing population of the world. Innovative products of trending technology in the field of agriculture, nanotechnology, contribute a significant boost for food production. The shortage of fresh water can be managed by adopting different efficient irrigation methods, also promote the quality and quantity of agricultural crops. By keeping in view, the above all, an experiment was conducted to evaluate the effect of nano nitrogen (nN) under different water regimes and assessed the growth attribute and other essential nutrient uptake by lettuce plant in different combination of bulk and nano nitrogen forms with surface and drip irrigation method. In this experiment, for the comparison of different irrigation methods, two control treatments were chosen, such as 100% bulk size nitrogen (bN) in surface irrigation and 100% bN application by drip irrigation. While nN was applied with bN in different combinations through drip irrigation and foliar application. Among all the combinations of nitrogen (N) fertilizer, application of 75% nN through drip irrigation and 25% of nN in foliar application significantly affect the growth and biochemical parameters such as plant biomass, leaf area, absolute growth rate, net assimilation rate, β-carotene, crude protein and yield. Similarly, N uptake, N use efficiency and apparent N recovery were increased by this combination as compared to lower N rates. The results indicated that the combined application of nN as a soil and foliar treatment was more efficient than that of soil application of bN. Furthermore; it could minimize the required N fertilization rate to reduce environmental pollution without any yield loss.
... Nano fertilizers can increase the contents of cellular chlorophyll, thereby increasing the rates of photosynthesis (Singh and Kumar, 2017). Still, on the opposite side, they minimize the undesirable impacts of biotic and abiotic stresses on seed germination (Tantawy et al., 2015;Giorgetti et al., 2019). Also, stated that using nanoparticles raised crop nutrient usage, minimized ecological pollution, and improved plant growth by introducing plant resistance and nutrient absorption. ...
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Ten-year-old lemon ( Citrus limon L. cv. Eureka) was used during the 2019 and 2020 seasons to investigate the effect of AgNPs at control, 5, 7.5, and 10 mg/L as a foliar application on vegetative growth, yield, and fruit quality. The selected trees were subjected to agricultural practices applied in the field during the study. The results indicated that the foliar application of AgNPs positively improved the shoot length, total chlorophyll, flower, and fruit set percentage, fruit yield, physical and chemical characteristics of fruits, and leaf mineral composition from macro and micronutrients compared to control in both seasons. The foliar application of AgNPs at 10 mg/L showed the highest mean values followed by 7.5 and 5 mg/L, respectively, for the previous characteristics. The treated leaves and fruit peels were hydrodistillated to extract the essential oils (EOs), and GC–MS analysis of leaf EOs. The analysis of leaves EOs showed the presence of neral, geranial, neryl acetate, and limonene as the main abundant bioactive compounds. While in peel the main compounds were neral, geranial, neryl acetate, D -limonene, geraniol acetate, linalool, and citronellal. Toxin effect of both EOs from leaves and peels were evaluated on the rice weevils ( Sitophilus oryzae ) and the results indicated a higher effect of lemon peel EOs than leaves based on mortality percentage and the values of LC 50 and LC 95 mg/L. Melia azedarach wood samples loaded with the produced lemon EOs were evaluated for their antifungal activity against the molecularly identified fungus, Fusarium solani (acc # OL410542). The reduction in mycelial growth was increased gradually with the applied treatments. The most potent activity was found in lemon leaf EOs, while peel EOs showed the lowest reduction values. The mycelial growth reduction percentages reached 72.96 and 52.59%, by 0.1% leaf and peel EOs, respectively, compared with control.
Conference Paper
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Bu çalışmada, farklı borik asit dozlarının arı otu (Phacelia tanacetifolia Bentham) bitkisinin çimlenme ve fide gelişimi üzerine etkilerinin belirlenmesi amaçlanmıştır. Araştırma, Siirt Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Laboratuvarı'nda, 25±1 °C kontrollü şartlar altında yürütülmüştür. Çalışmanın bitkisel materyalini "Sağlamtimur" arı otu çeşidi oluşturmuştur. Laboratuvar çalışması, tesadüf parselleri deneme desenine göre 4 tekrarlamalı olarak petri kaplarında kurulmuştur. Araştırmanın konusunu; borik asitin (H3BO3) 0.5, 1.0, 2.0, 3.0 ve 4.0 mM konsantrasyonu ile hidro-priming ve priming yapılmayan kontrol grupları teşkil etmiştir. Denemede 7. günün sonunda her bir Petri kabındaki bitkilerden rastgele seçilen 10 bitki üzerinden ölçümler yapılmıştır. Çalışmada; çimlenme oranı, ortalama çimlenme süresi, çimlenme üniformite katsayısı, çimlenme enerjisi, çimlenme indeksi, fide yaş ağırlığı ve fide güç indeksi parametreleri incelenmiştir. Yapılan istatistiki değerlendirmeler sonucunda, borik asit priming uygulamasının çimlenme ve fide gelişim parametrelerinin tamamını anlamlı derecede etkilediği belirlenmiştir. Araştırma sonuçlarına göre; çimlenme oranı % 65.3-92.0, ortalama çimlenme süresi 2.40-3.10 gün, çimlenme üniformite katsayısı 21.3-38.6, çimlenme enerjisi 2.7-21.3, çimlenme indeksi 6.47-12.30, fide yaş ağırlığı 25.99-35.08 mg ve fide güç indeksi 8.49-16.11 arasında değişim göstermiştir. Borik asit ile priming uygulamalarının arı otu bitkisinde olumlu tepkiler verdiği, çimlenme ve bazı fide gelişim özelliklerini iyileştirdiği görülmüştür. Arı otunda, çimlenme ve fide gelişimi için tohumların ekim öncesinde 3.0 mM borik asit konsantrasyonu ile ön işleme tabi tutulması önerilmektedir.
The rapid population growth and environmental challenges in agriculture need innovative and sustainable solutions to meet the growing need for food worldwide. Recent nanotechnological advances found its broad applicability in agriculture's protection and post-harvesting. Engineered nanomaterials play a vital role in plant regulation, seed germination, and genetic manipulation. Their size, surface morphology, properties, and composition were designed for controlled release and enhanced properties in agriculture and the food industry. Nanoparticles can potentially be applied for the targeted and controlled delivery of fertilizers, pesticides, herbicides, plant growth regulators, etc. This help to eliminate the use of chemical-based pesticides and their water solubility, protect agrochemicals from breakdown and degradation, improve soil health, and naturally control crop pathogens, weeds, and insects, ultimately leading to enhanced crop growth and production capacity in the food industry. They can be effectively utilized for nano-encapsulation, seed germination, genetic manipulation, etc., for protecting plants and improving crop productivity, safe and improved food quality, and monitoring climate conditions. Nanoparticles played a crucial role in the uptake and translocation processes, genetically modifying the crops, high seed germination, and productivity. In this article, we have reviewed some important applications of nanoparticles for sustainable agro-food systems. The need and role of nanotechnology concerning challenges and problems faced by agriculture and the food industry are critically discussed, along with the limitations and future prospects of nanoparticles.
Although, silicon – the second most abundant element in the earth crust could not supersede carbon (C) in the competition of being the building block of life during evolution, yet its presence has been reported in some life forms. In case of the plants, silicon has been reported widely to promote the plant growth under normal as well as stressful situations. Nanoform of silicon is now being explored for its potential to improve plant productivity and its tolerance against various stresses. Silicon nanoparticles (SiNPs) in the form of nanofertilizers, nanoherbicides, nanopesticides, nanosensors and targeted delivery systems, find great utilization in the field of agriculture. However, the mechanisms underlying their uptake by plants need to be deciphered in detail. SiNPs are reported to enhance plant growth, majorly by improving photosynthesis rate, elevating nutrient uptake and mitigating reactive oxygen species (ROS)-induced oxidative stress. Various studies have reported their ability to provide tolerance against a range of stresses by upregulating plant defense responses. Moreover, they are proclaimed not to have any detrimental impacts on environment yet. This review includes the up-to-date information in context of the eminent role of SiNPs in crop improvement and stress management, supplemented with suggestions for future research in this field.
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Tomato plants (Lycopersicon esculentum Mill.) cvs. Edkawy (salt tolerant) and T5 (salt sensitive) were tested for ethylene evolution and ammonium accumulation in the leaves by growing them in water culture either containing sea salt or supplied with N in the form of ammonium sulfate. Salinity treatments were 0.0 (control), 75 and 150 mM while ammonium sulfate treatments were 0, 5, 10, 15, 20, 25, 30, 35, 40 and 45 mM and both started on the fifth leaf stage. Ethylene production and ammonium accumulation increased with increasing ammonium sulfate concentration and it was greater in T5 variety than Edkawy. The same trends for ethylene evolution, ACC and ammonium accumulation were also clear with increasing salt concentration where it increases all determined aspects. Such result was clearly evident in T5 variety than Edkawy. Using Co++ salt alleviated symptoms of ammonium toxicity in necrotics form.
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This work was designed to investigate the alleviation of salinity negative effects by application of calcium in two different forms to tomato plants hybrid Super strain B irrigated with saline water with an EC 5.47 dS/m. Chelated calcium (CaO-EDTA 14%) or nano calcium (nano calcium carbonate 80.2%) forms were applied to the plants through drip irrigation system in two concentrations (2.0 & 3.0 g/l and 0.5 & 1.0 g/l for CaO-EDTA and Nano Ca respectively). Salinity showed the common negative effects on different plant growth parameters and yield. However both Ca compounds treatments alleviated those negative effects with a superior significant effect for nano calcium treatment of 0.5 g/l compared to all other treatments. Plant fruit yield and nutritional status were significantly improved under nano calcium treatments with superior effect of 0.5 g/l concentration.
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In a trail to ameliorate the negative effects of salinity on plant growth and production, this study was conducted during the growing seasons of 2012 and 2013 to investigate the interactive effect of chelated Fe-EDHHA 6% and salinity. Seedlings of tomato plant (Lycopersicon esculentum L.) hybrid Super strain B (salinity sensitive hybrid) were grown in pots and irrigated with saline solution in different concentrations (3000, 4000 and 5000 ppm). Under all salinity concentrations, Plants were supplied an aqueous solution of Fe-EDHHA 6% at four rates i.e. (0.0, 7.14, 9.52 and 11.9 kg/ha) divided into three doses. Data showed that as salinity level increased, all plant growth parameters i.e. plant height, leaf area, chlorophyll content, fresh weight and total yield were negatively and significantly affected. Chemical contents such as N, P and K were significantly reduced as well as plant production. Fe-EDHHA 6% application ameliorated salinity negative effects on plant growth and production as the application rate increased. The highest effect of Fe-EDHHA 6% was recorded with the application rate of 9.52 kg/ha which was not significantly different compared to the highest application rate of 11.9 kg/ha.
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Two field experiments were carried out in the two successive seasons of winter of 2007 and 2008 in the new Salhia region, Sharkia governorate. Seedlings of a salinity sensitive tomato hybrid Forbella (Lycopersicon esculentum L.) were cultivated in substrate slabs and irrigated with saline solution with different EC strengths namely (3000, 4000 and 5000 ppm). Irrigation was supplied until enough drainage was collected with the targeted EC of the treatment. Treatments of Amino acids compounds and growth regulator substance "Thidiazuron" were sprayed in concentration of 0, 2, 3 g/l and 0, 0.5 and 1 ppb (part per billion) respectively. Data showed that all growth parameters such as plant height, leaf area, total chlorophyll and K+contents, fresh weight of areal parts and percentage of dry weight of areal parts, as well as yield and some yield quality parameters responded negatively as the salinity level increased. Only Na+contents in the leaves and TSS in the fruits responded positively to the increment in salinity levels. The individual application of amino acids and "Thidiazuron" alleviated these negative effects. The alleviation effects were more pronounced as the concentration of the two substances increased. The highest alleviation effect was recorded with the highest concentration of Thidiazuron.
Conference Paper
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Soil and/or water salinity adversely affects growth and production of many crops. Salinity has a negative effect on plant water uptake which eventually affects growth and yield. However, improving shoot conditions by manipulating greenhouse climate (potential transpiration ETo) may improve plant water status and mitigate this negative effect. To test this hypothesis, climate in two identical greenhouse compartments was controlled normally in one (HET, high evapotranspiration) and with 25% less ventilation opening in the other to have lower vapor pressure deficit while preserving the same assimilation level. During summer, additional humidification was applied when necessary in the second compartment (LET, low evapotranspiration) so that ETo was not to exceed 0.15 l/pl.h. In each compartment, half of the rows of sweet pepper crop cv. Mazurka were fed, four weeks after transplanting, with 2 dS/m nutrient solution (LEC, low EC) while the other half was fed with 6.5 dS/m (HEC, high EC). Under high salinity, vegetative growth (number of leaves and total leaf area/plant) were enhanced by LET. HEC increased fruits infected with blossom end rot (BER) in the two greenhouses, while LET increased this fraction more by increasing the total number of fruits/plant. Salinity conditions also reduced significantly the number of marketable fruits. Plant total fresh and dry weights were significantly improved under saline conditions by greenhouse climate manipulation (LET). Mineral contents (%) of N, P, K, Ca and Mg were not significantly affected by the treatments. In conclusion, greenhouse climate manipulation can mitigate the effect of high EC in the root zone.
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Cucumber plants (Cucumis sativus) cv. Corona were grown in recirculating nutrient solution containing either 10 mg l-1 SiO2 (low Si) which was the level present in the water supply or given an additional 100 mg l-1 SiO2 (high Si). Silicate was depleted from the solution when cucumbers were grown, but accumulated when tomatoes were grown. Major effects on cucumber leaves of added Si were: increased rigidity of the mature leaves which had a rougher texture and were held more horizontally; they were darker green and senescence was delayed. The mature high Si leaves acquired characteristics of leaves grown in a higher light intensity, i.e. they had shorter petioles and an increased fresh weight per unit area, dry weight per unit area, chlorophyll content, RuBPcarboxylase activity and soluble protein (all expressed per unit area of interveinal laminar tissue). Addition of Si did not affect the final leaf area of the mature leaves but root fresh weight and dry weight were increased. A pronounced effect of Si addition was the increased resistance to the powdery mildew fungus Sphaerotheca fuliginea. Despite regular applications of fungicide, outbreaks of the fungal disease occurred on most of the mature leaves on the low Si plants, while the high Si plants remained almost completely free of symptoms. The addition of Si could be beneficial to cucumbers grown in areas where the local water supply is low in this element, especially when grown in recirculating solution or in a medium low in Si, e.g. peat.
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Three range grasses viz., Bothriochloa pertusa (L.) A. Camus, Dichanthium annulatum Forssk and Panicum antidotale Retz were subjected to 5, 10 and 15 dSm -1 and control (0.21 dSm -1) salinity levels in the laboratory. Germination percent, early seedling growth rate and moisture contents were determined. Salinity reduced germination in Panicum antidotale more than Bothriochloa pertusa and Dichanthium annulatum at all levels of applied salinity. The fresh weight of all the three grasses significantly declined specially in high concentration. The reduction in dry weight was obvious only at 15dSm -1 in all the tested grasses.
The effects of exogenous NaCl and silicon on activities of antioxidative enzymes in the root, shoot and leaf of two alfalfa cultivars were investigated in two alfalfa (Medicago sativa L.) cultivars: the high salt tolerant Zhongmu No. 1 and the low salt tolerant Defor. Both cultivars were grown in a hydroponics system with control (no NaCl and no Si added), Si treatment (1 mM Si), NaCl treatment (120 mM NaCl), and Si and NaCl treatment (120 mM NaCl + 1 mM Si). After 15 days of the NaCl and Si treatments, four plants of the cultivars were removed and divided into root, shoot and leaf parts for activity of antioxidative enzyme measurements. Salinity changed the antioxidative enzyme activity to different extents in the root and shoot of both cultivars when Si was not applied. Applying Si to both cultivars under NaCl stress significantly increased ascorbate peroxidase (APX) activity in root, shoot and leaves, and catalase (CAT) activity in leaves, and peroxidase (POD) activity in shoots of both cultivars, but decreased the superoxide dismutase (SOD) activity in shoots of Zhongmu No.1 and roots of both cultivars under salt stress. It is concluded that the changes of antioxidative enzymes activity varied in different organs of alfalfa plant after salt stress, while silicon could alter the activity of antioxidative enzyme of one or several organs of plants to improve the salt tolerance.