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In vivo and in vitro antitumor activity of jasminum sambac (Linn) ait oleaceae flower against dalton's ascites lymphoma induced swiss albino mice

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Abstract

The present study was aimed to evaluate the anticancer effect of Jasminum sambac against Daltons ascites lymphoma induced Swiss albino mice in in vitro and in vivo model. The tumor cell proliferation inhibitory activity of methanolic extract showed dose dependent in both HeLa and mouse fibroblast cells. At concentrations 25-400μg/ml, the percentage of cell inhibition concentration of normal and cancer cells was found to be 123.3 and 93.8 μg/ml respectively. The assessment of anticancer activity of J.sambac was evaluated by measuring the activity of hematological profiles, liver function marker and cancer marker enzymes. The methanolic extract at oral dose of 100mg/kg body weight exhibited a significant (p< 0.05) changes in the levels of hematological profiles, AST, ALT, ACP, ALT and LDH and cancer marker enzymes such as 5'Nucleotidase, β-D- Glucuronidase, γ-Glutamyl transferase as compared to DLA induced group. Thus it could be concluded that the methanolic extract of J.sambac possesses significant anticancer properties.

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... Treatment of EAC-bearing mice with SA-CMC brought back the hemoglobin content and RBC, WBC and platelets counts more or less to the normal levels. Kalaiselvi et al. [43] used jasminum sambac flower against Dalton lymphoma ascites (DLA) and Mazumder et al. [44] used Cucurbita maxima against Herlich ascites carcinoma and both research groups reported similar observations. Glutathione, a potent inhibitor of neoplastic process present in high concentration in liver, plays an important role as an endogenous antioxidant and was known to have the key function in the protective process against lipid peroxidation [42]. ...
... These serum enzymes activeties were significantly restored to near normal levels after treatment with SA-CMC, and a reduction in tumor metastasis. Similar to that, reduction of all elevated enzymes (P < 0.05) to near normal levels in animals treated with both Jasminum sambac and 5' fluorouracil [43]. ...
... Cancer is an abnormal type of tissue growth in which the cells exhibit an uncontrolled division, relatively in an autonomous fashion, leading to a progressive increase in the number of dividing cells [4]. It is a major public health problem worldwide and is the second leading cause of death in the world [5,6]. ...
... Dalton's lymphoma ascites (DLA) is a transplantable, poorly differentiated malignant tumor which appeared originally as lymphocytes in the mouse. It grows as both solid and ascitic forms [4]. ...
Article
Objective: The objective of this study is to investigate the anticancer potential of zinc oxide nanoparticles (ZnO-NPs) and hydroethanolic extract of Turbinaria conoides (HETC) against Dalton’s lymphoma ascites (DLA) cell line. Methods: Nanoparticles were synthesized from the HETC. An ultraviolet-visible spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology, and elemental composition of ZnO-NPs were also analyzed using scanning electron microscope-energy dispersive X-ray diffraction. The cytotoxic activity of ZnO-NPs and HETC was evaluated by 3-(4,5-dimethythiazol-. 2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue dye exclusion assays on DLA cells. The apoptosis inducing the effect was observed through acridine orange staining method (AO and EB) and DNA ladder assay. Results: The results of in vitro cytotoxic studies by MTT and trypan blue dye exclusion assays on DLA cell line in the presence of ZnO-NPs showed an IC50 value of 23.13 and 25.81 μg/ml, respectively. The DNA ladder assay and AO/EB staining clearly demonstrated that the ZnO-NPs at 50 μg/ml concentration induced a maximum apoptosis in DLA cells when compared with HETC. Conclusion: In the present study, the cytotoxic and apoptotic inducing effect of the synthesized ZnO-NPs and HETC were assessed, and it was found that ZnO-NPs possessed potent anticancer effect against DLA cells.
... In India, it is used as a tonic and also as a cure for ringworms (Singh et al. 2021). Several oleaceous plants, such as O. europaea (Zaïri et al. 2020;Essafi Rhouma et al. 2021) and other Jasminum species (Jantova et al. 2001;Hue Ngan et al. 2008;Kalaiselvi et al. 2011;Wei et al. 2021) was reported to have potent cytotoxic and anticancer activities. Unfortunately, few researchers have investigated the biological activities of J. humile, and little is reported regarding its phytochemical constituents. ...
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Context: Traditionally, Oleaceae plants are used to treat many diseases, such as rheumatism, hypercholes,terolaemia, or ulcers. Objectives: To investigate the cytotoxic potential of Jasminum humile L., Jasminum grandiflorum L., and Olea europaea L. (Oleaceae) extracts against selected human cancer cells lines, followed by a phytochemical investigation of the most potent one. Materials and methods: The 95% ethanol extracts of aerial parts of three oleaceous plants were examined for their cytotoxicity against HepG-2, MCF-7, and THP-1 cell lines using MTT assay and doxorubicin (positive control). J. humile was bio-selected and submitted to bio-guided fractionation. Chromatographic workup of ethyl acetate and n-butanol fractions afforded two new compounds; 1-methoxyjasmigenin (1) and 1-methyl-9-aldojasmigenin (2), along with five known ones (3–7). Structures were unambiguously elucidated using 1D/2D NMR and ESI-HRMS. Isolated compounds were assessed for their anti-proliferative potential, and both selectivity index and statistical significance were determined. Molecular docking was conducted against the Mcl-1 receptor using (AZD5991) as a standard. Results: Jasmoside (5) was the most potent anticancer compound showing IC50 values of 66.47, 41.32, and 27.59 mg/mL against HepG-2, MCF-7, and THP-1 cell lines, respectively. Moreover, isojasminin (4) exhibited IC50 values of 33.49, 43.12, and 51.07 mg/mL against the same cell lines, respectively.Interestingly, 5 exhibited the highest selectivity index towards MCF-7 and THP-1, even greater than doxorubicin. Molecular docking results were in full agreement with the MTT assay and the proposed SAR. Conclusion: In this study, two new compounds were purified. The biological activity highlighted jasmoside (5) as a lead anticancer drug for further future investigation.
... Free radical is any atom with atleast one unpaired electron in the outermost shell and is capable of independent existence. Free radicals and other reactive species produced during aerobic metabolism in the body can cause oxidative damage of amino acids, lipids, proteins and DNA [7] . It has been established that oxidative stress is among the major causative factors in the induction of many chronic and degenerative diseases including atherosclerosis, ischemic heart disease, ageing, diabetes mellitus, cancer, immunosuppression, neurodegenerative diseases and others. ...
... Jasminum sambac is also used as an antibacterial [13], antioxidants [8]. A study of Jasminum sambac conducted by Kunhachan et al. showed that the ethanol extract of white jasmine flower has endothelium-dependent relaxation activity by stimulating the release of nitric oxide from the endothelium of blood vessels through muscarinic receptors, and the methanol extract showed white jasmine flowers and cytotoxic anticancer effects [9]. From research conducted by Lestari that white jasmine (Jasminum sambac (L.) W.Ait.) ...
... It has a broad traditional and medicinal applications against many diseases (El-Hawary et al. 2019;El-Hawary, EL-Hefnawy, EL-Raey, et al. 2020;Elhawary, El-Hefnawy, Mokhtar, et al. 2020;Bhangale et al. 2012). J. officinale extracts have been reported to inhibit hepatitis B Virus (HBV) infection (Zhao et al. 2009) and control proliferation of lymphoma cells, both in vitro and in vivo (Kalaiselvi et al. 2011). Moreover, HCV plays a major role in developing extra hepatic solid tumours (Elemeery 2018;Youssef et al. 2018), that shed the light on the strong demand for developing novel drug leads with strong potential to control the key player proteins responsible for aggravation of the HCV correlated tumours initiation and metastasis. ...
Article
Jasminum multiflorum Burm. f. (J. multiflorum) is an ornamental plant with traditional medicinal importance. This study aims to evaluate the activity of J. multiflorum isolated compounds against hepatocellular carcinoma cells infected with hepatitis C virus (HCV) in vitro. The in vitro anti-viral and anti-oncogenic-related activity were validated by anchorage-independent assay plus transwell migration/invasion and spreading assay. In addition to chromatographic isolation of the active metabolites. The flower extract demonstrated a significant antiviral potential through reducing active viral replication by more than 90%. Study results credit this to specific reduction of viral NS5A and cellular EphA2 protein levels. Molecular docking analysis proved the role of the isolated compounds especially multifloroside, jasfloroside A and jasfloroside B as possible anti HCV molecules.
... At concentrations, 25-400 g/mL cell inhibition was observed with maximum growth inhibition of 93.8%. This anticancer activity was found in HeLa cells [44]. ...
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Background: There is a long history of traditional medicine for serving the world population. For the prevention and treatment of cancer, herbal remedies have played a significant role. To summarize medicinal herbs from the entire world, including India, that is used traditionally for various cancer treatment. Whenever we talk about cancer treatment, medicinal plants always have been on the priority. Objective: In this article, we have summarized the flora used in earlier times and recently identified for pre-clinical anticancer treatment. The present paper is a comprehensive review of different literature sources with discussion being made on the therapeutic value of diverse medicinal herbs in the treatment of various kinds of cancer by using different in vitro cancer cell lines. Countless anticancer plants have been recognized with the help of innovative techniques including isolation and structure elucidation that implement their beneficial effect by increasing the immunity of the body,inducing antioxidant action, endorsing making of shielding enzymes, hindering cancer triggering enzymes and hormones,and exciting DNA restoration mechanism. Conclusion: Finally, we have concluded that Argemone mexicana is found to show maximum anti-cancer activity on various cancer cell lines in comparison to other medicinal plants.
... The seeds can be used against round worms and threadworms, coughs, flatulence, intestinal colic and it can acts as pesticide. 3 The function of antioxidant in human body is to give protection against free radicals by scavenging them. 4 Free radicals are responsible for desolation of protein as well as lipid and DNA by oxidative process during aerobic metabolism in the biological system 5 which lead to many degenerative diseases and chronic diseases along with atherosclerosis, carcinogenesis, diabetes mellitus, ischemic cardiac disease, immunocompromised and neurodegenerative diseases. ...
... The methanolic extract of J. sambac flower revealed cytotoxicity on tumor and normal cells. The results exhibited that the methanolic extract of J. sambac flower reduced cell viability achieving cytotoxicity to HeLa cancer cells and minimum cytotoxicity towards the mouse embryonic fibroblasts normal cells [13]. The results from this current study demonstrate the cytotoxicity on fibroblast cell. ...
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Jasminum sambac (J. sambac) has been used as a medicinal herb with wound healing property. This experimental research proposed to evaluate the effect of J. sambac flower extracts on fibroblast cell viability and film characteristics which maybe benefit in development of wound dressing film. The testing extract of J. sambac flowers were prepared as filtration extract (F), compression of the residue flower extract (C), combination of filtration and compression extract (FC) and ethanolic extract (E). The testing extracts were evaluated the effect on fibroblast cell viability using resazurin reduction assay. NIH 3T3 fibroblast cells were treated with variable concentration of J. sambac extracts for 24, 48 and 72 h. prior to evaluate cell viability. The results demonstrated that the F and FC extract of J. sambac flower at concentration of 31.25-500 µg/ml did not induce cytotoxicity on NIH 3T3 fibroblast cells given non-cytotoxic effect. The cell viability seemed to increase at 48 h of treatment with concentration of 31.25-250 µg/ml. Meanwhile, C and E extract induced cytotoxicity on NIH 3T3 cells by reduction of cell viability. The F and FC non-cytotoxic extracts were selected to examine ability of film formation without film-based compounds and assessed physical and mechanical characteristics of the prepared films. The experimental results revealed F and FC extracts could form film after incubation at 60°C. The better physical and mechanical characteristics of the films was presented in FC film which the prepared film revealed smooth surface and better absorption and elongation. Furthermore, gelatin was blended to FC extract for assess improvement of film properties. The results revealed that gelatin-blended FC extract film gave better mechanical characteristics of the film. Non-cytotoxicity of the FC extract of J. sambac flowers on fibroblast cells and appropriate physical and mechanical characteristics of FC film may point to the usefulness of J. sambac flower extracts in herb-based film for wound dressing.
... Research work of Manokaran et al 23 , using plant extracts of Jasminum sambac, anti cancer activity on HeLa cells revealed percentage of cell death ranges from 17 to 98%. In this study, using plant extracts of Jasminum polyanthum, percentage of cell death ranges from 35 to 57% for different concentrations of leaf and flower extracts. ...
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Jasmine plant species are widely grown in Asia and used for religious offering, is known to have wide range of bioactive compounds and its properties. Most of the jasmine species have been evaluated its bioactive properties and found positive results. This work consists of evaluating bioactive properties of jasmine species, Jasminum polyanthum and finding its potential medicinal uses. The plant leaves and flowers were powdered and added water to prepare extract. Both leaves and flower extract was evaluated for phytochemical compounds, anti oxidant, anti diabetic, anti inflammatory, anti microbial, anti cancer and DNA nicking assay. The leaf and flower extract contained most of the phytochemical compounds which leads to presence of various bioactive properties. Leaf possesses good DPPH activity, while flower possess high phenol content and FRAP activity. Leaf possesses good anti diabetic activity, while flower possess good anti inflammatory activity. Flower extract consists of higher antibacterial activity than leaf, while in antifungal it is vice versa. Keywords: Anti-diabetic, Anti-inflammatory, Antioxidant activity, MTT Assay, DNA Nicking study
... The reduction in the activities of both γ-GT and ALT after treatment with both natural SOD-like activity protein and LMWH compared with those of the tumorized untreated mice can confirms the anti-tumor effect of the two materials. Similar to that, reduction of the activities of the all elevated enzymes (P<0.05) to near normal levels in animals treated with both Jasminum sambac and 5'fluorouracil were observe [59] . ...
... Several studies were performed on its antidiabetic [18], antiinflammatory [19], vasodilator activity [20] and effect on morphine withdrawal symptoms [21]. Additionally, extracts of the flowers were reported to exhibit cytotoxic activity towards brine shrimp Artemia [22], Hep-G2 [23] and Dalton's ascites lymphoma [24]. ...
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Objective: Evaluation of two Jasminum sambac L. (Ait) cultivars; Arabian Nights (JSA) and Grand Duke of Tuscany (JSG) ethanolic leaves extracts as reducing agents for the green synthesis of silver nanoparticles (AgNPs) and evaluation of their cytotoxicity against MCF-7 breast cancer and 5637 bladder cancer cell lines and chemical profiling of the two cultivars. Methods: The synthesis of silver nanoparticles (AgNPs) by the two cultivars and characterization of AgNPs by ultraviolet (UV)–visible spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). Additionally, the use of The high-performance liquid chromatography coupled with photodiode array-mass-mass-spectroscopy (HPLC-PDA-MS/MS) for chemical profiling of both cultivars and evaluation of total leaves extracts and corresponding nanoparticles towards MCF-7 and 5637 cell lines compared to aneuploidy immortal keratinocyte (Ha Cat) normal cells by neutral cell assay. Results: The green synthesized AgNPs (of an average size range of 8.83 and 11.24 nm for JSA and JSG, respectively) exhibited cytotoxicity against MCF-7 and 5637 cell lines. The IC50 was determined for each total extract JSA (15.29±2.16 μg/ml) and JSG (20.28±1.20 μg/ml) and corresponding AgNPs 17.32±2.22 μg/ml and 6.32±1.01μg/ml for JSA and JSG, respectively. The IC50 of JSA and JSG against 5637 bladder cancer cell line were 13.76±1.11 μg/ml and 50.69±3.75 μg/ml, while the corresponding AgNPs showed IC50 of 5.54±0.88 μg/ml and 27.89±2.84 μg/ml, respectively. The HPLC-PDA-MS/MS allowed the identification of 59 compounds; 10 simple phenols, 17 flavonoids; quercetin and kaempferol glycosides, 2 lignans, and 30 secoiridoids; oleuropein, molihauside, and sambacoside. Conclusion: This study proved that JSA is an excellent source for the synthesis of AgNPs with optimum characters and enhanced activities toward MCF-7 and 5637 cell lines in correlation to identified compounds.
... They are commonly known as free radical scavengers [13,14]. Free radicals produced in oxidative stress cause potential destruction of many important cellular components such as proteins, DNA, lipids, etc [15]. These type of destructions may result in a number of diseased conditions like carcinogenesis, age-related diseases, diabetes mellitus, neurodegenerative diseases, etc [16]. ...
... The reduction in the activities of both g-GT and SGPT after treatment with both natural SOD-like activity protein and LMWH compared with those of the tumorized untreated mice can confirm the anti-tumor effect of the two materials. Similar to that, reduction of the activities of the all elevated enzymes (p < 0.005) to near normal levels in animals treated with both Jasminum sambac and 5'fluorouracil were observe [55]. ...
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