The present study was aimed to evaluate the anticancer effect of Jasminum sambac against Daltons ascites lymphoma induced Swiss albino mice in in vitro and in vivo model. The tumor cell proliferation inhibitory activity of methanolic extract showed dose dependent in both HeLa and mouse fibroblast cells. At concentrations 25-400μg/ml, the percentage of cell inhibition concentration of normal and cancer cells was found to be 123.3 and 93.8 μg/ml respectively. The assessment of anticancer activity of J.sambac was evaluated by measuring the activity of hematological profiles, liver function marker and cancer marker enzymes. The methanolic extract at oral dose of 100mg/kg body weight exhibited a significant (p< 0.05) changes in the levels of hematological profiles, AST, ALT, ACP, ALT and LDH and cancer marker enzymes such as 5'Nucleotidase, β-D- Glucuronidase, γ-Glutamyl transferase as compared to DLA induced group. Thus it could be concluded that the methanolic extract of J.sambac possesses significant anticancer properties.