Twenty-seven compounds were obtained from the 95% ethanolic extract of aerial part of Gymnosporia varialilis Loes, and identified as prinsepiol (1), (+) - pinoresinol (2), (-) - pinoresinol (3), (-) - syringaresinol (4), (+) - cycloolivil (5), (-) - olivil (6), 1 - hydroxypinoresinol (7), ilelatifol D (8), scopoletin (9), β - amyrin acetate (10), α - amyrin acetate (11), fupenzic acid (12), (+) - catechin (13), secoisolariciresinol (14), palmitate - 1 - raonoglyceride (15), phytol (16), caffeic acid (17), 3,4 - dimethoxycinnamic acid (18), (-) - massoniresinol (19), tanegool (20), β - sitosterol (21), lupeol (22), betulin (23), β - daucosterol (24), oleanolic acid (25), ursolic acid (26) and (-) - olivil monoacetate (27).