Article

Megfigyelések csirkék adenovírus okozta zúzógyomorfekélyével kapcsolatban

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Abstract

The authors studied two large-scale broiler chicken farms (A and B) where, contrary to the appropriate housing and feeding conditions, the feed consumption decreased, anorexia, retarded growth, anaemia and feather abnormality were experienced. On farm A, the rearing period extended by 4 days, the mortality rate increased by 4% and the mean slaughter weight decreased by 0.5 kg. On farm B, the rearing period did not, but the mortality rate increased by about 7%. The elevated mortality level appeared between 10-15 days and 10-21 days on farm A and B, respectively. Post mortem examination revealed that the lumen of the proventriculus, the gizzard and almost the whole length of the intestine was filled with brownish-red creamy content. In the gizzard, the overlaying keratinoid layer on mucosa was partly detached, brownish-red discoloured and bloody infiltrated. After removing the keratinoid layer, erosions of the mucosa were observed in a diameter of about 0.5-2.0 cm. Histopathological findings included the degeneration or desquamation of the keratinoid layer, necrotic mucosa, degeneration and disappearance of epithel cells, and oedematous lamina propria infiltrated by lymphocytes, macrophages and heterophils. In the affected epithel cells basophilic intranuclear inclusion bodies were observed. Cytopathogenic effect typical of adenoviruses was experienced on the inoculated tissue cultures. Presence of adenoviruses was confirmed by PCR in the supernatant of the passaged tissue culture and in the mucosa of the affected gizzards. The isolated adenoviruses proved to be FAdV-1 by phylogenetic analysis of the sequences of the PCR product. The judgement of the economic impact of the gizzard erosion is a complex problem. In order to get a correct diagnose, it is always necessary to consider all details, including the anamnesis of a flock, the results of clinical and post mortem examination, histopathology, virus isolation and identification.

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... Tanimura et al. [1] reported adenoviral gizzard erosion for the first time from a natural outbreak in a flock of layer chickens. Subsequent reports generally originated from broiler flocks in Japan, but more recently also in Europe and Korea [2][3][4][5][6][7][8][9]. Experimental reproduction of adenoviral gizzard erosion by infection with FAdV-1 isolated from field outbreaks has been reported in specificpathogen-free (SPF) layers as well as in commercial broilers. ...
... Similar to this, decreased weight gain was described by Okuda et al. [11] in an experimental study of 1-week-old and 3-week-old commercial broilers. The generated data as well as information collected from field outbreaks [6,8,9] confirm that growth retardation in the course of FAdV-1 induced gizzard erosion may present a serious problem for the economic success of broiler production. Similar to the majority of other experimental studies set up to reproduce gizzard erosion, no other clinical signs were observed in the present investigation. ...
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