Improvement of peripheral blood circulation in mouse and human with aged garlic extract preparation combined with ginseng, oriental bezoar, antler velvet, cuscuta seed and epimedium herb

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We newly produced an aged garlic extract preparation combined with ginseng, oriental bezoar, antler velvet, cuscuta seed and epimedium herb (LEOPINROYAL; LER), and examined whether it would improve peripheral blood circulation, as determined by the cooling-rewarming test in mice, or by a four-week trial in humans. In the mouse cooling-rewarming test, LER accelerated recovery from a fall in rectal temperature induced by cooling treatment (15°C for 10 min), and LER was especially superior in controlling the rate of increase in rectal temperature at 30 min after administration and beyond (p<0.01). In the four-week human trial, LER (1 ml) was taken twice a day after meals in the morning and evening. The condition of the peripheral blood circulation was estimated 0, 2 and 4 weeks after taking LER using two instruments: a BC-Checker for measuring the peripheral blood circulation index, and a ASTRIM SU for measurement or visualization of the width of small veins. In consequence, LER was found to improve the peripheral blood circulation index and the width of small veins. We tried to analyze in detail the extent of change among different groups (sex, blood circulation index at initial point, change of condition by interview, etc.), and it was noteworthy that women, a group who claimed they felt good upon interview after LER treatment, and a group showing lowering of the blood circulation index at initial point were highly responsive. In conclusion, the present results indicate that LER improves the condition of the peripheral blood circulation, suggesting that it acts as a preventive or care agent to resolve various kinds of problems, such as a stiff shoulders or lower back pain, caused by disorders of the peripheral blood circulation.

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... In another clinical study, AGE has been shown to enhance cutaneous microcirculation in patients with the increased risk of cardiovascular events (42). In addition, an animal study demonstrated that AGE can reverse the decrease in the rectal temperature of mice induced by cooling (32,43). Furthermore, administration of S1PC, a characteristic constituent of AGE increased the blood flow of tail skin in spontaneously hypertensive rats (SHR) (21). ...
Aged garlic extract (AGE) has been shown to improve peripheral circulatory disturbances in both clinical trials and experimental animal models. To investigate the effect of S-1-propenylcysteine (S1PC), a characteristic sulfur compound in AGE, on cold-induced reduction in tail blood flow of rat, Wistar rats were individually placed in a restraint cage and given the treatment with cold water (15˚C) after the oral administration of AGE or its constituents S1PC, S-allylcysteine (SAC) and S-allylmercaptocysteine (SAMC). After the cold-treatment the tail blood flow of rats was measured at the indicated times. The pretreatment with AGE (2 g/kg BW) and S1PC (6.5 mg/kg BW) significantly alleviated the reduction of rat tail blood flow induced by cold treatment. The effect of S1PC was dose-dependent and maximal at the dose of 6.5 mg/kg BW, whereas SAC and SAMC were ineffective. To gain insight into the mechanism of S1PC action, the concentration of nitrogen oxide metabolites (NOx) in the plasma and the levels of phosphorylated endothelial nitric oxide synthase (eNOS) and 5'-AMP-activated protein kinase (AMPK) in the aorta were measured. The pretreatment with S1PC significantly increased the plasma concentration of NOx as well as the level of phosphorylated form of AMPK and eNOS in the aorta after cold-treatment. The present findings suggest that S1PC is a major constituent responsible for the effect of AGE to alleviate the cold-induced reduction of peripheral blood flow in rat by acting on the AMPK/eNOS/NO pathway in the aorta.
The effect of several kinds of garlic preparations such as raw garlic juice (RGJ), heated garlic juice (HGJ), processed garlic powder (PGP) and aged garlic extract (AGE) on both physiological and psychological stress were investigated using four stress models in mice: forced swimming test, mechanical treadmill running, immobilization stress test, and a cooling rewarming test. RGJ was shown to be effective only at a low dose in the forced swimming test, whereas the effect was reduced at a high dosage. HGJ and PGP demonstrated no antistress effects. In contrast, AGE was shown to be effective in all of the stress tests. © 1997 by John Wiley & Sons, Ltd.