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Clinical effects of fish type I collagen hydrolysate on skin properties

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... Oral supplementation with hydrolyzed collagen inhibited the loss and the fragmentation of collagen by aging in rats, in part by inducing the expression of type I and type III collagen, as well as inhibiting MMP expression and activity (1,3). In pigs, hydrolyzed collagen intake increased collagen fibrils' density and diameter (11). Similar effects on the stimulation of collagen synthesis were observed in cultured fibroblasts isolated from UV-exposed body areas (12). ...
... Nestes estudos, foi notado que a suplementação oral com colágeno hidrolisado inibiu a perda e a fragmentação de colágeno pelo envelhecimento em ratos, em parte por induzir a expressão de colágeno tipo I e tipo III, além de inibir a expressão e atividade de MMP (1,3). Em suínos, a ingestão de colágeno hidrolisado resultou em um aumento da densidade e do diâmetro das fibrilas de colágeno (11). Efeitos semelhantes na estimulação da síntese de colágeno foram observados em fibroblastos cultivados de áreas isoladas do corpo expostas a radiação UV (12). ...
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The effect of collagen peptides on various aspects of skin and hair physiology is known but needs further studies. Thus, the objective of this study was to evaluate the clinical changes in skin and hair after 90 days of treatment with oral supplementation of 5 g/day of collagen peptides. For this, 60 healthy female participants were enrolled, aged 45 to 60 years old, with the primary objective of evaluating the effect of the ingestion of a bioactive collagen peptides-based supplementation in the cutaneous microrelief, reduction of wrinkles, thickness, and echogenicity of the dermis, as well as in the mechanical properties of the hair using biophysical and skin imaging techniques. The present study showed important benefits in relevant skin visual parameters, dermis density, and hair strength with the obtained data. In addition, considering that the aging process affects the hair's mechanical resistance due to hair fiber thinner, the proposed treatment was effective for aged hair. In conclusion, the collagen peptides oral supplementation is essential not only for improving skin conditions but also for hair care once it significantly increases the mechanical hair resistance evaluated by objective measurements. Keywords: Collagen peptides, skin aging, skin imaging techniques, hair mechanical properties, clinical study
... More research is needed to verify the efficacy and safety of collagen hydrolysate intake by conducting controlled clinical trials 4,33,34 . In some clinical studies, the oral administration of collagen hydrolysates was ineffective against photoaging skin 35,36 , and the number of volunteers or the completeness of the design was insufficient 37 . The other clinical trials were also conducted with collagen hydrolysates extracted from pigs 24,[38][39][40] or chickens 41,42 , not from fish. ...
... In addition, some clinical studies have proved that simply enzymatically hydrolyzed collagen products without revealing the bioactive peptide component exert beneficial effects on skin hydration 36,38,43,44 . Above all, most clinical studies conducted oral supplementation with physiologically active ingredients such as antioxidants, vitamins, and collagen hydrolysate rather than collagen hydrolysate intake alone 37,[45][46][47][48] . To the best of our knowledge, this is the first study to estimate the photoaged skin improvement efficacy through a randomized, double-blinded, placebo-controlled clinical study design using fish-derived collagen hydrolysates containing specific bioactive dipeptide forms, Gly-Pro, and Pro-Hyp not combined with any antioxidants and vitamins. ...
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Collagen hydrolysate, which contains bioactive peptides, is used as a dietary supplement for the refinement of elasticity, hydration, desquamation, and wrinkling of aging human skin. Here, we conducted a double-blind, randomized, and placebo-controlled oral administration study on the effects of a collagen peptide (CPNS) containing dipeptides, including Gly-Pro and Pro-Hyp, on skin wrinkling, desquamation, elasticity, and hydration. Our results show that an intake of 1650 mg per day of CPNS for 12 weeks had beneficial effects on skin health in a cohort of women aged from 30 to 60 years (n = 100). Compared with the placebo group, skin desquamation, hydration, skin wrinkling, and elasticity were significantly improved after 4, 4, 12, and 12 weeks of administration, respectively. In a safety test of CPNS ingestion, none of the participants showed any side effects during the clinical study period. These results demonstrate that the low molecular weight bioactive peptides contained in CPNS, such as Gly-Pro and Pro-Hyp, exert positive effects on skin hydration, elasticity, desquamation, and wrinkling.
... Previous investigations have revealed that oral administration of collagen hydrolysates on subjects with dry and rough skin showed that the moisture content, elasticity, and smoothness of the skin was greatly improved while the appearance of wrinkles and skin roughness was decreased [6]. Asserin et al. explored the effects of a daily oral supplement containing collagen peptides on skin hydration and the density of collagen. ...
... The skin holds 70% of collagen which provides skin tissues with considerable tensile strength. However, as ageing progresses, the decline of collagen accelerates particularly in women [6]. ...
... In the recent years, the relationship between nutrition factors and skin function has received increasing attention. Animal experiments and preclinical human trials investigating the effects of oral supplementation with collagen peptides have indicated the possibility that dietary compounds can modulate skin function (16)(17)(18)(19)(20)(21). For example, Matsuda et al. (18) reported that in pigs a 9-week oral ingestion of collagen hydrolysates induced increased fibroblast density and enhanced formation of collagen fibrils in the dermis in a protein-specific manner. ...
... In a pilot trial (not double-blind and not Placebocontrolled study), an oral daily ingestion of 5g of hydrolysed fish collagen mixed with vitamin C (50 mg) and glucosamine (60 mg) improved skin hydration and elasticity on face and forearms of female (19). In a double-blind and Placebo-controlled study, Proksch et al. (22) reported on the effects of specific porcine collagen peptides (2,5g/day and 5g/day) on face skin assessed as dry. ...
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Objective: To assess the anti-aging potential of three type I fish collagen hydrolysates (CH1=Naticol® BPMG, CH2=Naticol® HPMG, CH3=Naticol® 1000MG) on skin aging signs for three different body sites of mature women. Design: Double-blind, randomized and Placebo-controlled clinical study. Setting: Centre of Clinical Pharmacology Applied to Dermatology (CPCAD, Nice). Participants: Sixty women aged 46-69 years having skin aging signs on the face. Intervention: Participants were randomized to receive a once daily 5g dose of one of the CHs or Placebo for 8 weeks. Measurements: Skin biomechanics, skin hydration and visual assessment of the crow’s-feet wrinkles were evaluated after 4 and 8 weeks of treatment. Subject satisfaction questionnaire and Investigator global efficacy appreciation (IGEA) were also used. Results: Skin biomechanics indicated a significant improvement of skin firmness for the three CHs compared to Placebo, in particular for CH2. An increase of overall skin elasticity for CH3 (p = 0.017) and CH2 (p = 0.044) on the abdomen was also observed. This was corroborated by the significant decrease of the crow’s-feet wrinkle score at week 8 for both CH3 and CH2 (p=0.023 and p=0.014, respectively). Concerning the self-questionnaire, overall the number of positive responses was significantly higher for CH2 compared to Placebo and other CHs. For the IGEA, the number of favorable answers was greater for CH2 than for the Placebo group (80% vs. 36%, p= 0.025). A positive influence of CH treatments could be observed for skin hydration but failed to reach statistical significance. Conclusion: The tested type I fish collagen hydrolysates have beneficial effects on skin quality. In particular, CH2 demonstrated the greatest range of these effects including improvement of skin biomechanics, decrease of wrinkles, good subject satisfaction and no related adverse events.
... In conclusion, they suggested that fish-derived hydrolyzed collagen can be safely used to improve skin health in an aging population [14]. In a study with female subjects diagnosed with dry and rough skin, six weeks of daily intake of 7 g of fish hydrolyzed collagen was reported to significantly improve the moisture content of the skin in face-cheek, forearm, and the back of the neck and enhance the skin elasticity by reducing wrinkles and lowering skin roughness [44]. In another place-controlled study, the use of 2.5 or 5 g hydrolyzed collagen for 60 days was reported to improve skin elasticity significantly and skin moisture compared to placebo, while the improvement in elasticity was noticed among older women relatively earlier (at 1-month follow-up) [19]. ...
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Background: This study aimed to evaluate the efficacy and safety of oral hydrolyzed collagen peptide (HCP) in healthy females by assessing the skin parameters via biophysical and skin imaging techniques. Methods: 112 females were randomly assigned to receive either HCP (n = 57; 10 g CollaSel Pro®) or placebo (n = 55; 10 g maltodextrin) daily for eight weeks. The contribution of HCP to skin elasticity, hydration, and roughness was investigated against a placebo, while the facial soft tissue sagging (RMS) and safety data were also recorded. Results: HCP was associated with significant improvements in skin elasticity (p = 0.009), skin hydration (p ranged from 0.003 to <0.001), and skin roughness (p ranged from 0.002 to <0.001). In the HCP vs. the placebo group, week eight values for skin elasticity (43.0 (7.4) vs. 40.3 (3.3) mPa, p = 0.017), skin hydration (65.8 (18.9) vs. 53.1 (14.9) g/m³, p < 0.001) and skin roughness (40.2 (20.4) vs. 24.9 (20.9) g/m³, p < 0.001) were significantly higher. In the HCP group, week 8 RMS values were significantly lower than baseline values (1.02 (0.21) vs. 1.10 (0.21) mm, p = 0.012). Conclusions: CollaSel Pro® HCP can be considered a well-tolerated, safe product that effectively improves dermal health and the appearance of sagging and ameliorates the signs of the aging process.
... At 14 days after the administration of [14C] Gly-Pro-Hyp, almost all radioactivity disappeared from the organs, except for the skin, with a radioactivity of 70% observed after 6 h [71]. Another similar study observed radioactivity after a single administration of [14C] Gly-Pro-Hyp in the connective tissues including the bones and skin within 24 h [72]. ...
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This paper presents a systematic review and meta-analysis of 26 randomized controlled trials (RCTs) involving 1721 patients to assess the effects of hydrolyzed collagen (HC) supplementation on skin hydration and elasticity. The results showed that HC supplementation significantly improved skin hydration (test for overall effect: Z = 4.94, p < 0.00001) and elasticity (test for overall effect: Z = 4.49, p < 0.00001) compared to the placebo group. Subgroup analyses demonstrated that the effects of HC supplementation on skin hydration varied based on the source of collagen and the duration of supplementation. However, there were no significant differences in the effects of different sources (p = 0.21) of collagen or corresponding measurements (p = 0.06) on skin elasticity. The study also identified several biases in the included RCTs. Overall, the findings suggest that HC supplementation can have positive effects on skin health, but further large-scale randomized control trials are necessary to confirm these findings.
... Due to the extensive functions of type 1 collagen throughout the body, there has been interest in the effects of collagen-based dietary supplements on health-and performance-related outcomes (Zdzieblik et al. 2015;Shaw et al. 2017;Zdzieblik et al. 2017;Dressler et al. 2018;Lis et al. 2022). For example, it has been shown that daily supplementation with 5-10 g of collagen peptides or hydrolyzed collagen enriched with vitamin C for three weeks to six months decreased wrinkles and improved smoothness, pliability and elasticity of the skin (Matsumoto et al. 2006), reduced joint pain at rest and during exercise (Clark et al. 2008;Zdzieblik et al. 2017), increased ankle stability (Dressler et al. 2018), and decreased fat mass, as well as increased fat free mass, muscle strength (Zdzieblik et al. 2015), the rate of force development during isometric squats, and vertical jump height (Lis et al. 2022). ...
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Type 1 collagen is an abundant structural protein with importance to the skin, eyes, bones, ligaments, tendons, and muscles. Shilajit supplementation has been shown to increase gene expression of collagen synthesis, however, it is unclear if increased gene expression translates to increases in circulating levels. Therefore, the purpose of the present study was to examine the effects of 8 weeks of daily supplementation with 500 mg·d⁻¹ and 1000 mg·d⁻¹ of Shilajit versus placebo on serum pro-c1α1, a biomarker of type 1 collagen synthesis. Thirty-five recreationally trained men (mean ± SD: age = 21.1 ± 1.8 yrs; body mass = 80.7 ± 12.4 kg; height = 180.9 ± 6.7 cm) volunteered to participate in this study. Mixed factorial and one-way ANOVAs were used to analyze mean differences between groups, with follow-up t-tests when necessary. Individual subject responses were assessed using the minimal clinically important difference and Chi-squared tests. There were significant (Low dose: p = 0.008, d = 1.2; High dose: p = 0.007, d = 1.3) increases in serum pro-c1α1 from pre- (Low dose: 42.5 ± 12.4 ng·mL⁻¹; High dose: 42.7 ± 12.7 ng·mL⁻¹) to post-supplementation (Low dose: 82.3 ± 46.5 ng·mL⁻¹; High dose: 113.1 ± 78.7 ng·mL⁻¹) for the low and high dose groups, however, no change (p > 0.05) for the placebo group. A greater proportion (p = 0.03) of subjects exhibited increases in pro-c1α1 that exceeded the minimal clinically important difference in the high dose Shilajit group (75%) compared to the placebo group (30%), but no differences (p = 0.06) between the low dose Shilajit group (69%) and placebo. In conclusion, 8 weeks of Shilajit supplementation with 500 and 1000 mg·d⁻¹ increased type 1 collagen synthesis as indicated by serum levels of pro-c1α1.
... [15] Collagen is used in cosmetics and biomaterials much extensively than elastin. Human ingestion trials with collagen hydrolysate increased moisture content of the skin in healthy women [16]. Food-derived collagen peptides, such as prolyl-hydroxyproline (Pro-Hyp) detected in human peripheral blood following ingestion, enhanced fibroblast growth and the synthesis of the extracellular matrix, both of which were seen to be beneficial for the skin [17]. ...
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To achieve the precise quantitation of desmosine (DES) and isodesmosine (IDS) in skipjack tuna “Katsuo” elastin, an isotope dilution liquid chromatography tandem mass spectrometry (LC–MS/MS) method was applied for the samples of elastin-containing products in tablet, drink, jelly, and capsule forms, and the results were compared with data obtained from an amino acid (AA) analyzer. The quantity of DES/IDS was found to be ca. 2860 mg/kg for elastin peptide A, and 1690 mg/kg for elastin peptide B. Thus, the DES/IDS ratio of 100 ± 5% was obtained for the LC–MS/MS values compared to the data provided by the (AA) analyzer excepting jelly and capsule. This work justifies the quantification of DES/IDS in elastin products by LC–MS/MS, contributing to its application in the agro-food industry.
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Malnutrition is one of the main causes of bone and cartilage disorders. The emergence of food supplements to increase skin quality and anti-aging has become a topic of interest for researchers, aesthetic specialists and the general public. Therefore, new ingredients with Functional properties are of interest for the development of new functional beverages. One of the notable ingredients of the food industry is hydrolyzed collagen, which contains 8 essential amino acids and glycine, and the concentration of proline is nearly 20 times higher than other protein-rich foods. In humans and animals, 25% of their body protein is in the form of collagen. Due to the reduction of collagen synthesis in the body, collagen demand for skin, hair and bone tissues increases with age. A highquality biological peptide is marine collagen, which is also known as "super collagen" because it is the collagen that our body can absorb in the best way. An intestinal product is produced. It has been clinically tested in Japan that the use of hydrolyzed collagen increases the softness, hydration and elasticity of the skin. The results show that collagen drink can exert intensive anti-aging effects on inhibiting ROS, facilitating extracellular matrix (ECM) protein synthesis, and increasing mitochondrial activity. In short, the synergistic effect of collagen drink can not only reduce oxidative damage. reduce, but also improve cell function to compensate for the harmful effects caused by UVA.
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