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106
Indian Journal of Weed Science 47(2): 106–109, 2015
Bio-efficacy of different herbicides for weed control in direct-seeded rice
Simerjeet Kaur* and Surjit Singh
Department of Agronomy, Punjab Agricultural University, Ludhiana, Punjab 141 004
Received: 5 April 2015; Revised: 8 June 2015
ABSTRACT
Field experiment was conducted during Kharif 2009 and 2010 to study the bio-efficacy of different
herbicides in direct-seeded rice. Weed control treatments comprised of pendimethalin 0.75 kg, butachlor
1.50 kg, thiobencarb 1.50 kg, anilofos 0.375 kg, pretilachlor 0.75 kg, oxadiargyl 0.09 kg and pyrazosulfuron
ethyl 0.015 kg/ha as pre-emergence and with sequential application of bispyribac 0.025 kg/ha at 30 DAS;
two hand weedings and unweeded control. Significantly lower number of grass weeds was observed
with application of pendimethalin as compared with other pre-emergence herbicides. Sequential
application of pendimethalin and bispyribac recorded the lowest weed biomass and 100% weed control
efficiency. Crop dry matter accumulation, number of tillers, and effective tillers were significantly higher
in sequential use of pre- and post-emergence herbicides, resulting in more grain yield and net returns.
The maximum grain yield was recorded in two hand weedings, which was at par with follow-up
application of bispyribac after pendimethalin, butachlor, thiobencarb and oxadiargyl.
Key words: Direct-seeded rice, Economics, Grain yield, Herbicides, Weed control efficiency
Weeds are the main constraint in direct-seeded
rice since the inherent weed control from standing
water at crop establishment is lost (Rao et al. 2007).
In direct-seeded rice, high weed infestation causes
grain yield losses up to 90%. Weeds pose a serious
threat by competing for nutrients, light, space and
moisture just from the time of emergence and
throughout the growing season, whereas weed seeds
germinate after rice transplanting in transplanted rice
and compete with the well-established rice seedlings.
A change in crop establishment method from
transplanting to direct seeding brings about changes
in the weed community; grasses – Dactyloctenium
aegyptium, Digitaria ciliaris, Eragrostis spp.,
Eleusine indica, Acrachne racemosa, Commelina
benghale-nsis; sedges - Cyperus compressus, Cyperus
rotundus and broad-leaved –Digera arvensis,
Phyllanthus niruri, Amaranthus viridis and
Trianthema portulacastrum have also started
appearing in rice fields along with Echinochloa crus-
galli, Echinochloa colona, Leptochloa chinensis,
Cyperus iria, Cyperus difformis, Eclipta alba,
Sphenochloa zeylenica etc. So, conversion from
transplanted to direct-seeded rice results in more
competitive weed flora requiring revised weed
management approaches for effective control. A
weed-free period for the first 25-45 DAS is required
to avoid any loss in yield in dry direct-seeded rice
(Chauhan and Johnson 2011, Singh et al. 2012).
Hand weeding operations are laborious, time
consuming, uneconomical, difficult and moreover,
result in uprooting of some rice seedlings due to
difficulty in differentiating grass weeds that mimic
rice plants during early growth. Some herbicides
found effective in DSR are pendimethalin, cyhalofop-
butyl, fenoxaprop-ethyl, propanil, bispyribac-sodium,
penoxsulam, carfentrazone-ethyl, bensulfuron,
metsulfuron + chlorimuron, azimsulfuron and 2,4-D.
In Punjab state, seven pre-emergence herbicides
namely pendimethalin, butachlor, thiobencarb,
anilofos, pretilachlor, oxadiargyl and pyrazosulfuron-
ethyl have been recommended in puddled
transplanted rice. Identifying herbicides with wide-
spectrum weed control ability for efficient and
economical weed management is crucial for
improving the potential of direct seeding of rice.
Keeping this in view, an experiment was conducted to
study the bio-efficacy of different pre- and post-
emergence herbicides used in conventional puddled
transplanted rice in direct-seeded rice.
MATERIALS AND METHODS
The field experiment was conducted at
Research Farm, Department of Agronomy, Punjab
Agricultural University, Ludhiana during Kharif
season of 2009 and 2010. Ludhiana is situated in
Trans-Gangetic Agro-Climatic zone, representing the
Indo-Gangetic Alluvial plains at 30°56' N latitude,
75°52' E longitude and at an altitude of 247 m above
mean sea level. The maximum temperature remained
above 38° C during summer. The total rainfall of 818
*Corresponding author: simer@pau.edu
107
and 627.6 mm were received during 2009 and 2010,
respectively. Most of the rainfall was received in
vegetative phase from 23rd (at sowing time) to 35th
standard meteorological week (80 days after
sowing). The soil of the experimental site was loamy
sand with normal soil reaction of pH 7.5 and electrical
conductivity of 0.16 dS/m. The soil was low in
organic C (0.31%) and available N (251.7 kg/ha) and
medium in available P (13.5 kg/ha) and K (164.1 kg/
ha). The crop was sown with conventional seed-
cum-fertilizer drill at 20 cm row to row spacing with
seed rate of 35 kg/ha.
Sixteen weed control treatments comprised of
pre-emergence application of pendimethalin 0.75 kg,
butachlor 1.50 kg, thiobencarb 1.50 kg, anilofos
0.375 kg, pretilachlor 0.75 kg, oxadiargyl 0.09 kg and
pyrazosulfuron-ethyl 0.015 kg/ha, and each unlined
with sequential application of bispyribac 0.025 kg/ha
at 30 days after sowing (DAS), two hand weedings
and unweeded control. Pre-emergence herbicides
were sprayed in moist field within two days of
sowing and bispyribac was sprayed at 30 DAS as
follow-up. Pre- and post-emergence herbicides were
applied with knapsack sprayer fitted with flat fan
nozzle using 375 L/ha of water. Nitrogen (187.5 kg/
ha) was applied as broadcast in four equal splits at 2,
4, 7 and 10 weeks after sowing. Phosphorus (30 kg/
ha), potassium (30 kg/ha) and zinc sulphate
heptahydrate (62.5 kg/ha) were applied at the time of
seed-bed preparation by broadcasting. Plant
protection measures for insect-pests and diseases
were taken to grow healthy crop.
Data on weed population and dry matter, crop
growth and yield were recorded. Weed control
efficiency and benefit:cost ratio were calculated.
Weed data were square-root transformed before
statistical analysis. Pooled analysis of two years was
done and comparisons were made at 5% level of
significance.
RESULTS AND DISCUSSION
Weed population and dry matter
The predominant weed flora of the experimental
field were: Echinochloa crusgalli, Echinochloa
colona and Cyperus iria, Cyperus difformis at 30
DAS prior to post-emergence application of
bispyribac. Along with Echinochloa spp., other grass
weeds like Digitaria sanguinalis and Dactyloctenium
aegyptium were also reported but Cyperus spp. was
not observed at later stages of observation as most of
the rainfall was received during vegetative phase of
crop which led to smothering and perishing of weed
flora. Pendimethalin treated plots recorded
significantly lower number of Echinochloa spp. than
all other herbicides but have no effect on Cyperus
spp. (Table 1). Other pre-emergence herbicides, viz.
butachlor, thiobencarb, anilofos, pretilachlor,
oxadiargyl and pyrazosulfuron-ethyl, conventionally
used in puddled transplanted rice under Punjab
conditions were broadcast in standing water as pre-
emergence but in this study, these were applied on
moist soil as spray application. Similar results were
obtained by Singh et al. (2009) that under dry seeding
methods, effective weed control was recorded with
pre-emergence application of pendimethalin than
pretilachlor. Statistically similar number of Cyperus
spp. was observed with application of all pre-
emergence herbicides, resulting in non-significant
differences in weed dry matter. But, weed dry matter
was at par in pyrazosulfuron-ethyl and unweeded
control. One hand weeding done at 25 DAS has
significantly lower weed dry matter as compared
with other weed control treatments at 30 DAS.
Follow-up application of bispyribac controlled
Echinochloa spp. and Cyperus iria but had no control
over D. sanguinalis and D. aegyptium, however, D.
sanguinalis and D. aegyptium were controlled with
Table 1. Effect of weed control treatments on weed population and dry matter at 30 DAS in direct-seeded rice (mean of
two years)
Treatment Echinochloa spp. (no./m2) Cyperus spp. (no./m2) Weed dry matter (g/m2)
Pendimethalin 0.75 kg/ha 3.2 (10) 6.4 (40) 5.8 (33)
Butachlor 1.50 kg/ha 4.6 (21) 5.5 (30) 6.3 (38)
Thiobencarb 1.50 kg/ha 4.5 (19) 5.7 (32) 6.3 (38)
Anilofos 0.375 kg/ha 4.5 (19) 5.9 (34) 6.7 (45)
Pretilachlor 0.75 kg/ha 5.3 (27) 5.7 (32) 6.4 (41)
Oxadiargyl 0.09 kg/ha 5.1 (25) 5.3 (28) 7.3 (53)
Pyrazosulfuron-ethyl 0.015
kg/ha 6.4 (40) 6.2 (38) 8.9 (79)
Hand weeding at 25 DAS 1.0 (0) 1.0 (0) 1.0 (0)
Unweeded 9.6 (92) 14.5 (210) 9.1 (83)
LSD
(P=0.05)
0.5
0.8
0.8
The data are square root transformed and values in the parentheses are original values.
Bio-efficacy of different herbicides for weed control in direct-seeded rice
108
two hand weeding and pre-emergence application of
pendimethalin, was significantly superior to other
herbicides and unweeded control (Table 2). This
resulted in lowest weed dry matter and 100% weed
control efficiency. Walia et al. (2008) also reported
that pendimethalin application in dry-seeded rice
provided effective control of weeds not associated
with paddy crop, whereas bispyribac controlled all
typical predominant weeds including Echinochloa
spp. and all Cyperus spp. Bispyribac-sodium and
other pre-emergence herbicides, butachlor,
thiobencarb, anilofos, pretilachlor, oxadiargyl and
pyrazosulfuron-ethyl did not control these grass
weeds. Similar results were reported by Kumar and
Ladha (2011). Follow-up spray of bispyribac after
butachlor, thiobencarb, anilofos, pretilachlor,
oxadiargyl and pyrazosulfuron-ethyl resulted in
significantly lower weed dry matter than alone
application of pre-emergence herbicides, resulting in
88% weed control efficiency. Single application of
pre-emergence herbicides showed poor weed control
efficiency (19.0–24.2%) (Table 2).
Crop growth and yield
Follow-up application of bispyribac 0.025 kg/ha
at 30 DAS after pendimethalin 0.75 kg, butachlor
1.50 kg, thiobencarb 1.50 kg, anilofos 0.375 kg,
pretilachlor 0.75 kg, oxadiargyl 0.09 kg and
pyrazosulfuron-ethyl 0.015 kg/ha registered
significantly taller plants as compared with single
application of pre-emergence herbicides, resulting in
more crop dry matter production and effective tillers
(Table 3). Better crop growth in these treatments
might be attributed to more availability of nutrients,
water, light and space to crop as a result of effective
weed control. All weed control treatments recorded
significantly more number of effective tillers as
compared with unweeded control. Single application
of pre-emergence herbicides, viz. pendimethalin,
butachlor, thiobencarb, anilofos, pretilachlor and
oxadiargyl recorded significantly higher number of
effective tillers as compared with pyrazosulfuron-
ethyl as pre-emergence and unweeded control.
Number of grains/panicle was significantly higher in
sequential spray of pre- and post-emergence
herbicides than single application of pre-emergence
herbicides, except pendimethalin, which was at par
with unweeded control. The maximum grain yield
was recorded in two hand weedings which was at par
with sequential application of pendimethalin,
butachlor, thiobencarb and oxadiargyl with
bispyribac. Follow-up application of bispyribac
registered significantly more grain yield as compared
with single application of pre-emergence herbicides.
Table 2. Effect of weed control treatments on weeds in direct-seeded rice (mean of two years)
Data are square root transformed and values in the parentheses are original values
Simerjeet Kaur and Surjit Singh
Treatment
Weed count at 60 DAS (no./m
2
) Weed dry matter
(g/m2) Weed control
efficiency
at
harvest (%)
Echinochloa
spp. Cyperus
sp. Digitaria
sanguinalis
Dactyloct-
einum
aegyptium 60 DAS At harvest
Pendimethalin 0.75 3.4 (13) 2.7 (6) 1.0 (0) 1.0 (0) 8.1 (66) 27.3 (759) 30.4
Pendimethalin 0.75
fb
bispyribac
0.025 1.0 (0) 1.0 (0) 1.0 (0) 1.0 (0) 1.0 (0) 1.0 (0) 100.0
Butachlor 1.50 4.0 (19) 2.6 (6) 1.9 (3) 1.8 (2) 10.1 (101) 28.5 (823) 24.2
Butachlor 1.50 fb bispyribac 0.025 1.0 (0) 1.0 (0) 1.9 (3) 1.7 (2) 4.2 (18) 11.1 (123) 88.6
Thiobencarb 1.50 4.0 (19) 2.6 (6) 1.9 (3) 1.7 (2) 10.0 (101) 29.3 (865) 20.4
Thiobencarb 1.50
fb
bispyribac
0.025
1.0 (0) 1.0 (0) 2.0 (3) 1.7 (2) 4.3 (18) 11.2 (126) 88.3
Anilofos 0.375 4.1 (19) 2.8 (7) 1.9 (3) 1.7 (2) 10.0 (101) 29.2 (876) 19.5
Anilofos 0.375 fb bispyribac
0.025 1.0 (0) 1.0 (0) 1.9 (3) 1.8 (2) 4.3 (18) 10.9 (121) 88.7
Pretilachlor 0.75 4.2 (20) 2.5 (5) 1.9 (3) 1.8 (2) 9.8 (96) 29.0 (851) 21.9
Pretilachlor 0.75
fb
bispyribac
0.025 1.0 (0) 1.0 (0) 1.9 (3) 1.7 (2) 4.4 (20) 11.4 (129) 88.0
Oxadiargyl 0.09 4.0 (18) 2.6 (6) 1.9 (3) 1.7 (2) 10.4 (107) 29.1 (855) 21.4
Oxadiargyl 0.09 fb bispyribac
0.025 1.0 (0) 1.0 (0) 1.8 (3) 1.7 (2) 4.4 (19) 10.8 (120) 88.8
Pyrazosulfuron-ethyl 0.015 4.3 (21) 2.7 (7) 2.0 (3) 1.8 (2) 10.4 (108) 29.5 (882) 19.0
Pyrazosulfuron-ethyl 0.015 fb
bispyribac 0.025 1.0 (0) 1.0 (0) 2.0 (3) 2.0 (3) 4.3 (18) 11.2 (126) 88.3
Two hand weeding 1.0 (0) 1.0 (0) 1.0 (0) 1.0 (0) 1.0 (0) 10.6 (112) 89.4
Unweeded 6.1 (39) 2.8 (7) 2.1 (4) 1.9 (3) 17.2 (294) 32.7
(1077) -
LSD (P=0.05) 0.3 0.3 0.3 0.4 0.9 2.2
109
These results are in conformity with the findings of
Walia et al. (2009) and Mahajan and Timsina (2011).
Walia et al.(2008) also reported that it is difficult to
raise weed-free DSR with the application of only one
herbicide. The dry matter of weeds and grain yield
has an inverse relationship with r value of -0.98.
Amongst the pre-emergence herbicides, only
pendimethalin recorded significantly more grain yield
as compared to unweeded control. Similar results
were obtained by Singh et al. (2009) that under dry
seeding, higher grain yield was recorded with pre-
emergence application of pendimethalin 1.50 kg/ha.
The difference in yield might be due to differences in
application mode and efficacy of herbicides against
weed species.
Economics
Higher B:C ratio was obtained when bispyribac
was sprayed as follow-up application after pre-
emergence herbicides as compared with single
application of pre-emergence herbicides. The highest
net profit was realized from sequential application of
pendimethalin and bispyribac, followed by sequential
application of thiobencarb/butachlor/oxadiargyl and
bispyribac (Table 3). Net returns under weedy
situation were negative (loss), which revealed that
weed control in DSR is an important component.
Similarly, except pendimethalin, all other pre-
emergence herbicides resulted in negative returns.
REFERENCES
Chauhan BS and Johnson DE. 2011.Row spacing and weed
control timing affects yield of aerobic rice. Field Crops
Research 121: 226-231.
Kumar V and Ladha JK. 2011. Direct seeding of rice: recent
developments and future research needs. Advances in
Agronomy 111: 297-413.
Mahajan G, Chauhan BS and Johnson DE. 2009. Weed
management in aerobic rice in North-western Indo-Gangetic
Plains. Journal of Crop Improvement 23(4): 366-382.
Mahajan G and Timsina J. 2011 Effect of nitrogen rates and
weed control methods on weeds abundance and yield of
direct-seeded rice. Archives in Agronomy and Soil Science
57(3): 239-250.
Rao AN, Johnson DE, Sivaprasad B, Ladha JK and Mortimer
AM. 2007. Weed management in direct-seeded rice.
Advances in Agronomy 93: 153-255.
Singh B, Malik RK, Yadav A and Nandal DP. 2009. Herbicide
efficacy in seeded rice with different methods under wet
and dry conditions. Indian Journal of Weed Science
41(1&2): 37-40.
Singh M, Sriram CV, Hanji MB, Prabhukumar S and Kishor N.
2012. Crop-weed competition and weed management
studies in direct-seeded rice (Oryza sativa). Indian Journal
of Agronomy 57(1): 38-42.
Walia US, Bhullar MS, Nayyar S and Sidhu AS. 2009. Role of
seed rate and herbicides on growth and yield of direct dry-
seeded rice. Indian Journal of Weed Science 41(1&2): 33-36.
Walia US, Bhullar MS, Nayyar S and Walia SS. 2008. Control of
complex weed flora of dry-seeded rice (Oryza sativa L.)
with pre- and post-emergence herbicides. Indian Journal
of Weed Science 40(3 &4): 161-164.
Table 3. Effect of weed control treatments on crop growth, yield attributes, yield and economics of direct-seeded rice
(mean of two years)
Treatment Final plant
height (cm)
Crop dry matter
accumulation
(g/m2)
Number of
effective
tillers/m2
Number of
grains/
panicle
Grain
yield
(t/ha) B:C
Pendimethalin 0.75 kg/ha 68.9
776.3
147.5
69.2
2.35
0.09
Pendimethalin 0.75 fb bispyribac 0.025 kg/ha 84.5
1257.0
291.3
84.8
5.56
1.38
Butachlor 1.50 kg/ha 70.3
732.2
123.8
64.4
1.41
-0.33
Butachlor 1.50
fb
bispyribac 0.025 kg/ha 85.0
1203.2
243.9
79.3
5.22
1.28
Thiobencarb 1.50 kg/ha 66.7
777.1
141.3
65.3
1.48
-0.30
Thiobencarb 1.50
fb
bispyribac 0.025 kg/ha 87.0
1236.7
285.8
79.5
5.29
1.31
Anilofos 0.375 kg/ha 68.5
751.8
133.3
63.8
1.44
-0.31
Anilofos 0.375 fb bispyribac 0.025 kg/ha 86.7
1206.6
265.4
77.9
4.66
1.05
Pretilachlor 0.75 kg/ha 68.2
704.2
128.7
64.3
1.48
-0.30
Pretilachlor 0.75
fb
bispyribac 0.025 85.4
1166.7
272.5
79.1
4.65
1.02
Oxadiargyl 0.09 kg/ha 67.0
687.3
135.0
65.0
1.49
-0.29
Oxadiargyl0.09 fb bispyribac 0.025 kg/ha 85.5
1199.9
277.4
80.4
5.22
1.28
Pyrazosulfuron-ethyl 0.015 kg/ha 62.9
479.0
60.3
57.7
1.23
-0.42
Pyrazosulfuron-ethyl 0.015 fb
bispyribac 0.025 kg/ha 86.3
1170.7
235.4
79.4
4.44
0.94
Two hand weeding 87.6
1252.1
295.1
83.0
5.64
1.12
Unweeded 62.5
331.9
38.8
57.1
1.21
-0.31
LSD (P=0.05) 7.3
104.6
40.1
6.6
0.46
Bio-efficacy of different herbicides for weed control in direct-seeded rice