Tobacco leaf eating caterpillar, Spodoptera litura is one of the most important insect pests of tobacco in nursery as well as in field. The biology of S. litura on different tobacco varieties, Anand-119, GT-s, GT-g, GTH-1, ABT-10 and ABD-101, was studied at 26+'1oC and75+1o/o relative humidity under laboratory conditions. All of the biological parameters included in the study were affected by the ... [Show full abstract] host varieties. The larval period ranged from 22 to 31 days on GTH-1 was longer than on other tobacco varieties. Fecundity was more on GT-9, least on ABD-1 01 and intermediate on Anand-'119, ABT-10, GT-s and GTH-'1. Pupal period, ddult period and growth index was significantly higher on GT-S than on others and larval mortality (%) was also significantly lower on GT-5 than other varieties. Pupal morlality (%) was lower on ABD-101 than on other varieties. The overall suitability of the cultivars for the development of S. titura,lhe fastest completion of life cycle (average 39.4 days) was recorded on tobacco cultivarABT-10 but on cultivar GTH-'l , it takes an average of 49.17 days to complete its life cycle.