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Prosexual Effects of Lycium Barbarum



Lycium barbarum, also known as wolfberry, has been commonly used as an oriental herb in China for a long period. Wolfberry is suggested to be antiaging and used to maintain health of kidneys, liver, and the eyes. In the practice of traditional Chinese medicine, wolfberry was also prescribed for patients who suffered from dysfunctions of sexual desire. Although it has been put into application for a long history, the underlying mechanism is still elusive. In the past decades, increasing lines of evidence support the roles of wolfberry in promoting male sexual functioning. The benefit of wolfberry was shown on different aspects including preventing the reproductive tissues from oxidative insult, improving motility of sperm, maintaining a testosterone level, and promoting sexual performance of the hemicastrated rats. This chapter discusses on the recent research findings, both from bench and bedside, which support the prosexual function of wolfberry, and discusses the potential clinical application of wolfberry on the treatment of sexual behavior.
Chapter 8
Prosexual Effects of Lycium Barbarum
Benson Wui-Man Lau, Mason Chin-Pang Leung, Kai-Ting Po,
Raymond Chuen-Chung Chang and Kwok-Fai So
B. W.-M. Lau () · R. C.-C. Chang
LKS Faculty of Medicine, Department of Anatomy, The University of Hong Kong,
Pokfulam, Hong Kong
B. W.-M. Lau · M. C.-P. Leung · K.-T. Po
Department of Rehabilitation Sciences, The Hong Kong Polytechnic University,
Kowloon, Hong Kong, People’s Republic of China
K.-F. So
LKS Faculty of Medicine, Department of Ophthalmology, The University of Hong Kong,
Pokfulam, Hong Kong, People’s Republic of China
R. C.-C. Chang · K.-F. So
The State Key Laboratory of Brain and Cognitive Science, The University of Hong Kong,
Pokfulam, Hong Kong, People’s Republic of China
R. C.-C. Chang · K.-F. So
LKS Faculty of Medicine, Research Centre of Heart, Brain, Hormone and Healthy Aging,
The University of Hong Kong, Pokfulam, Hong Kong, People’s Republic of China
R. C.-C. Chang · K.-F. So
GMH institute of Central nervous System Regeneration, Guangdong Key Laboratory of Brain
Function and Diseases, Jinan University, Guangzhou, People’s Republic of China
Abstract Lycium barbarum, also known as wolfberry, has been commonly used
as an oriental herb in China for a long period. Wolfberry is suggested to be antiag-
ing and used to maintain health of kidneys, liver, and the eyes. In the practice of
traditional Chinese medicine, wolfberry was also prescribed for patients who suf-
fered from dysfunctions of sexual desire. Although it has been put into application
for a long history, the underlying mechanism is still elusive. In the past decades,
increasing lines of evidence support the roles of wolfberry in promoting male sex-
ual functioning. The benefit of wolfberry was shown on different aspects includ-
ing preventing the reproductive tissues from oxidative insult, improving motility
of sperm, maintaining a testosterone level, and promoting sexual performance of
the hemicastrated rats. This chapter discusses on the recent research findings, both
from bench and bedside, which support the prosexual function of wolfberry, and
discusses the potential clinical application of wolfberry on the treatment of sexual
© Springer Science+Business Media Dordrecht 2015
R. C-C. Chang, K-F. So (eds.), Lycium Barbarum and Human Health,
DOI 10.1007/978-94-017-9658-3_8
114 B. W.-M. Lau et al.
Keywords Wolfberry · Lycium barbarum · Sexual behaviors · Mating ·
Reproduction · Aphrodisiac
The prosexual and fertility effect of wolfberry was first described by the Chinese
herbalist Li Shizhen (Wang et al. 2002). While in traditional Chinese medicine
(TCM) practice, wolfberry is commonly prescribed for sexual dysfunction, the ben-
eficial effect of wolfberry on reproduction provided by scientific methodology has
just investigated in the past two decades.
8.1 Beneficial Effect of Wolfberry on Sexual Behavior
The first evidence which supports the prosexual effect of wolfberry polysaccharide
on live animals was reported by Luo et al. (2006). Lycium barbarum polysaccharide
(LBP) treatment for 22 days was shown to attenuate the impaired copulatory per-
formance caused by hemicastration in rats, which is shown by markedly shortened
penis erection latency, mounts latency, and increased successful mount percentage.
Interestingly, the sexual performance of rats with LBP treatment is even slightly
better than that of normal rats. In parallel to the sexual behavior, sex hormones
in plasma and the weight of accessory reproductive organ are also maintained in
hemicastrated rats treated with LBP. To elucidate the protective effect at cellular
level, the authors also tested the protective effect of LBP on testicular tissues. When
being subjected to hyperthermia, rat testis tissues were found to have decreased
weight and superoxide dismutase (SOD) activity, which is associated with a struc-
tural damage of the seminiferous tubules. Pretreatment with LBP before the heat
exposure was able to prevent the abovementioned damage on the testicular tissue.
Furthermore, treatment with LBP in vitro also prevented the deoxyribonucleic acid
(DNA) damage caused by H2O2. These findings support the traditional Chinese
viewpoint that wolfberry is an aphrodisiac agent and may facilitate fertility by sci-
entific evidence.
Apart from TCM practice, wolfberry was also adapted as a remedy to improve
sexual functioning in Korea (Sohn et al. 2008). An herbal formulation consists of
the seeds of wolfberry and other herbs were tested for its effect on penile erection.
After treatment for 4 weeks, the intracavernosal pressure was found to increase
markedly in medicated animals, which indicate that erection was promoted. Simul-
taneously, an expression of nNOS and eNOS (neuronal and endothelial nitric oxide
synthase, respectively), which generate nitric oxide as a vasodilator, was also found
to be upregulated by the medication. These observations suggested that the herbal
mixture may promote male copulatory function by promoting penile erection.
A study conducted by Lau et al. tested whether the LBP could counteract the
sexual-suppressing effect of high dose corticosterone (Lau et al. 2012). As several
studies suggested that neurogenesis (i.e., production of new functional neurons)
8 Prosexual Effects of Lycium Barbarum
may be an essential mediator of sexual behavior and newborn neurons may have an
important role in the regulation of sexual behavior (Lau et al. 2011; Leuner et al.
2010), the authors examined whether neurogenesis takes roles in the prosexual ef-
fect of LBP and the association between LBP treatment, sexual behavior, and neu-
rogenesis. The results of the study indicated that LBP facilitated male sexual behav-
ior by significantly increased copulatory efficiency (CE) and ejaculation frequency
(EF), and decreased ejaculation latency (EL). LBP at dosage of 1 mg/kg showed the
most significant effect than a higher dosage of 10 mg/kg.
Furthermore, LBP also significantly reversed the inhibited sexual behav-
ior induced by corticosterone in intromission latency (IL) and CE and reversed
suppressed neurogenesis induced by corticosterone in terms of number of BrdU-
positive cells in subventricular zone (SVZ) and hippocampus. LBP also promotes
the neuronal differentiation of neural precursor cells as shown by DCX staining.
Moreover, the study further revealed that sexual performance is correlated with
neurogenesis in SVZ and hippocampus and the direct relationship is examined by
comparing BrdU-positive cells and sexual behavior between blocking neurogenesis
in LBP treatment group and control group. After blocking neurogenesis, the rats
showed impaired sexual performance, which indicate the necessity of neurogenesis
in sexual functioning. This study demonstrated the enhancing effect of LBP on male
sexual behaviors in rats, the association between neurogenesis and the causal rela-
tionship between neurogenesis and sexual behavior. As newborn neurons may have
an important role in the regulation of sexual behavior, this study suggests that LBP
may promote sexual behavior through the regulation of neurogenesis.
A randomized controlled clinical study was conducted to investigate the effect of
a drink which was prepared from wolfberry (“Goji Juice GoChi”) on general health
of human subjects (Amagase and Nance 2008). The drink was prepared from fresh
wolfberry, with a daily serving which is equivalent to at least 150 g of fresh wolf-
berry. Questionnaires were used to evaluate subjective well-being after consuming
the drink for 14 days. In comparing to the placebo group, the subjects who had
the wolfberry drink responded with a significantly higher energy level, feeling of
health and exercise performance, and reduction in fatigue and stress. Interestingly,
more than half of the female subjects consumed the juice reported decrease in pain
during their mentrual period, and a few subjects in the treatment groups stated they
had increased sexual ability and activity. Since these aspects on reproduction were
not the main foci of the randomized controlled trial (RCT), no dedicated instrument
and analysis were used to evaluate the difference between the treatment and pla-
cebo groups. Another study reported by the same research group indicated that after
chronic consumption of the wolfberry drink for 30 days, the in vivo antioxidant
markers including SOD and glutathione peroxidase (GSH-Px) in the plasma were
upregulated (Amagase et al. 2009), which exposed the antioxidant properties of the
wolfberry drink on human. As oxidative stress may be one of the underlying mecha-
nisms of sexual inhibition and infertility, the antioxidant action of wolfberry drink
in human may be linked to the prosexual effect reported by the previous study. Nev-
ertheless, the effect on the subjective feelings on human sexual function would be
116 B. W.-M. Lau et al.
worthwhile for future RCT experiment. As increasing lines of evidence showed the
prosexual effect of wolfberry in laboratory animals, further studies on the potential
reproductive effect of wolfberry on human would be valuable for the translation of
laboratory studies to clinical applications.
8.2 Protective Effect of Wolfberry on Reproductive
Since wolfberry has been shown to exert its effect by its antioxidant activity, it
was hypothesized that antioxidation is a mechanism that underlies the beneficial
effect of wolfberry on reproductive function. As a by-product of intracellular res-
piration, reactive oxygen species (ROS) are reactive molecules which may damage
the reproductive tissues if in excess amount (Balaban et al. 2005). ROS species
including hydroxyl ions, superoxide, and nitric oxides are generated in the testis
during steroidogenesis and spermatogenesis (Mathur and D’Cruz 2011). While an
appropriate level of ROS is required for the normal functioning of the testis tissue
and sperms, an excessive amount of ROS is detrimental to the sperms as the sperms
are highly sensitive to peroxidation due to the rich content of polyunsaturated fatty
acids (PUFA) on the plasma membrane. A proper balance between the ROS and an-
tioxidants is thus needed to maintain the functioning of the reproductive tissues and
sperms. As antioxidants were found in various edible plants (Clément et al. 2012),
it is suggested that plant-derived feed supplements may inhibit the oxidative stress
in livestocks and may promote both fertility and reproductive functions. One of the
food supplements that received increasing attention is wolfberry.
Different studies have investigated the protective effect of wolfberry on repro-
ductive tissues by both in vivo and in vitro methods, and tried to determine whether
wolfberry exerts its effect through its antioxidant properties. The protective effect
of wolfberry polysaccharides on testicular tissue was firstly shown in a tissue cul-
ture study (Wang et al. 2002). When seminiferous epithelium was cultured for a
prolonged period and at a relatively high temperature, structural degradation was
induced in terms of increase in intercellular space and appearance of multinucleated
spermatids, which is due to the impaired spermatogenesis. Inclusion of wolfberry
polysaccharide in the culture medium reduced the structural damage caused by the
lengthened culture time and hyperthermia. The spermatozoa were also found to be
more motile when treated with LBP. The reduced structural damage is accompanied
with a decrease in apoptotic rate of epithelium cells and reduction in oxidative stress
(illustrated by ultraviolet C light induced lipid peroxidation and superoxide induced
cytochrome c reduction). The authors suggest that the protective effect of LBP may
be due to its antioxidant properties.
In another study conducted by Zhang et al. showed that LBP could protect the
spermatogenesis in testis caused by Bisphenol A (BPA) (Zhang et al. 2013). BPA is
a commonly found monomer in plastic wares and widely used in adhesive and den-
8 Prosexual Effects of Lycium Barbarum
tal fillings (Podlipna and Cichna-Markl 2006). Despite its wide range of applica-
tions, BPA was found to decrease the weight of testis, induce apoptosis in spermato-
genic tissues, decrease testosterone levels and increase the rate of infertility in rats
(Li et al. 2009; Podlipna and Cichna-Markl 2006; Xiao et al. 2011). Interestingly,
LBP was shown to prevent the retardation of reproductive function in rats caused
by BPA. First, LBP was shown to increase the weight of testis and epididymis in
BPA-treated rats, although not to a level comparable to the normal controls. Sec-
ond, LBP was shown to prevent the decrease in the level of sex hormones caused
by BPA. Third, the proapototic marker, Bax was found to decrease after LBP treat-
ment. Finally, oxidative stress was also suppressed by the LBP. These data suggest
that LBP would mitigate the damage of testicular tissues from BPA by suppressing
apoptosis and oxidative stress, while further exploration of the fertility of the rats
after BPA and LBP treatment would support the use of wolfberry in reproductive
function against toxic agents on reproductive system.
Another study explored whether pretreatment with LBP could ameliorate the
detrimental effect on reproductive system caused by doxorubicin (DOX), an antitu-
mor drug used in the treatment of solid and hematological tumors (Xin et al. 2012).
Aside from its side effect on cardiopulmonary and excretory system (Minotti et al.
2004), DOX also causes toxicity on testicular tissue and spermatozoa manifested
in decreasing quantity and motility of sperm, increasing the rate of abnormal sperm
production and increasing apoptosis in the spermatogenesis process. Being similar
to the abovementioned studies, the authors hypothesized that oxidative stress is
a major underlying mechanism of the disturbed reproductive function. Evidences
indicated that the weight of the testis and epididymis in DOX-treated male rats was
preserved by LBP.
Morphologically, degenerative changes including depletion of germ cells, ir-
regular seminiferous tubules and scarcity of spermatogonia were caused by DOX,
which could be prevented by LBP. While LBP does not show any effect on sperm
quantity and quality in healthy rats, it effectively prevented the decline of sperm
quantity and quality which is induced by DOX. Furthermore, the increased oxida-
tive stress in testicular tissues caused by DOX, indicated by the level of malondi-
aldehyde (by-product of lipid peroxidation) and GSH-peroxidase (a scavenger of
free radicals), was attenuated by LBP. Again, the evidence suggests that LBP may
be a potential adjunct therapy for the protection of reproductive organs through the
regulation of oxidative stress.
Different lines of evidence showed that ionizing irradiation has damaging effects
in male reproductive system (Bonde 2010) and testicular spermatogenic cells were
highly sensitive to ionizing irradiation. Cancer patients receiving radiotherapy or
people working with radioactive substances often had infertility and sexual dys-
function (Hasegawa et al. 1997). A study attempted to elucidate whether LBP could
protect the reproductive tissues from ionizing irradiation-induced reproductive cell
damage (Luo et al. 2011). Male rats were exposed to various levels of ionizing ir-
radiation and then cotreated with LBP. After the experiment, sperm count, their mo-
tility, erection latency, sexual behavior, serum hormone, DNA damage in testicular
118 B. W.-M. Lau et al.
cells, and protein content of testicular tissues were tested. The result showed that in
irradiated animals, sperm count and their motility were significantly reduced. More-
over, erection latency, mounting, and ejaculation latencies were extended, serum
testosterone levels was lower and DNA damage in testicular cells were observed
after exposing to ionizing irradiation. The longer the exposure to ionizing irradia-
tion, the greater the deleterious effect will be found. Cotreatment with LBP was
shown to significantly alleviate the detrimental effect on the reproductive tissues.
The author suggested that LBP could repair the damage in testicular cells caused
by ionizing irradiation, regulate the serum testosterone level, and protect testicu-
lar cells against deleterious effects of free radicals caused by ionizing irradiation.
A subsequent study conducted by the same group showed that LBP upregulates
the expression of antiapoptotic protein Bcl-2, downregulated proapoptotic Bax
and maintain the mitochondrial membrane potential of testicular tissues (Luo et al.
2014), which suggests that LBP may have antiapoptotic effects against irradiation.
Owing to the traditional viewpoint that wolfberry is aphrodisiac and its availabil-
ity is at low cost, the potential profertility effect of wolfberry in female has drawn
the attention of research groups as early as the 1970s (Suzuki et al. 1972). In a study
conducted by Suzuki and coworkers, intravenous injection of crude water-soluble
extract of Lycium Chinense, another species of wolfberry or goji berry, induced
ovulation in rabbits (Suzuki et al. 1972). However, due to the lack of an appropriate
control group in the abovementioned study, whether the ovulation is induced by the
wolfberry extract or due to other confounds remains to be determined.
Huang et al. studied the effect of LBP on in vitro maturation of the female gam-
ete (Huang et al. 2008). Interestingly, when frozen oocyte with cryoprotection was
thawed and treated with LBP in culture conditions, the maturation rate was signifi-
cantly higher than the traditional sucrose medium. However, while the maturation-
promoting action was attributed to the influence on solution viscosity and osmolal-
ity, it is unknown of whether the effect is specific to the wolfberry.
In a case study, concentrated herbal extracts (Zuo-gui-wan), which contains
cooked wolfberry and other herbs such as dogwood fruit and cyathula root, was
used to treat a woman with premature ovarian failure and secondary amenorrhea
(Chao et al. 2003). After 3 months of therapy, ovulation return and the woman con-
ceived successfully.
Interestingly, wolfberry was found to benefit not only the adult animals but also
offsprings when they are exposed to it during the gestation period (Feng et al. 2010).
It was found that prenatal stress resulted in a significant decrease in cognitive func-
tion in female offspring, which could be prevented significantly by pretreatment
of mother rats with milk-based wolfberry. After the pretreatment with wolfberry
polysaccharide for 2 weeks, female rats were allowed to mate. Then, the pregnant
female subjects were restrained by a transparent plastic tube on days 14–20 of preg-
nancy three times daily from 45 min to 1 h. The female offspring were subsequently
tested at 1 month of age. Morris Water Maze was used to test the spatial memo-
ry and the offspring rats were sacrificed to test the oxidative brain mitochondrial
8 Prosexual Effects of Lycium Barbarum
damage. The result showed that prenatal restraint stress induced memory and learn-
ing deficiency of female offspring, but not in male offspring, while middle and high
doses wolfberry pretreatment significantly reduced the impairment. In vitro studies
by the authors showed that wolfberry dose dependently scavenged hydroxyl and
superoxide radicals and inhibited ascorbic acid-induced dysfunction in brain tissue
and tissue mitochondria.
When comparing to male, the effect of wolfberry on female reproductive func-
tion and offspring remains unclear and only a few limited reports studied the ef-
fect. There is a lack of supportive scientific evidence on the effect of wolfberry on
female sexual behavior and reproduction. However, as it was shown that wolfberry
could increase the levels of plasma sex hormones and protect the male gametes, it is
likely that female reproductive system may be benefited by the herb. The support-
ing evidence from empirical studies will definitely strengthen the clinical usage of
wolfberry in practice, and even as a food supplement.
In conclusion, evidence of L. barbarums prosexual effect has been found at
different levels of organisms, namely: molecular level, biochemistry level, cellular
level, tissue level, behavior level. Also, it improves the general well-being of hu-
mans. We have summarized all these effects in Table 8.1.
Apart from protecting the DNA and alleviating the reproductive tissue damage due
to hypothermia, oxidative stress, and radiation, the fruit also protects testis from
damage by environment pollutant like BPA. Female fertility is also restored by a
herbal medicine containing wolfberry. The fruit also shows protective effect to the
cognitive function of offspring with prenatal stress. Different pieces of evidence
have shown that L. barbarum would be an all-round aphrodisiac agent that not
only improves the sexual function of male individual and the fertility of female,
but also the general health of both male and female. These beneficial effects imply
L. barbarum would be a potent functional food. Nevertheless, the drug interaction
between the fruit and common drugs, especially drugs for chronic diseases, still
need to be clarified, in order to utilize wolfberry as an adjunctive therapy for sexual
dysfunctions. Yet, further studies may be needed to determine the therapeutic dos-
age of wolfberry as the quality control of the fruit and the active components are
still being elusive.
120 B. W.-M. Lau et al.
Sex of
Species or experimen-
tal model
Effect of wolfberry component Reference
Male ♂ Mating Rats, hemicastrated Shortened penis erection latency, mounts latency Luo et al. 2006
Increased successful mount percentage
Rats Promote penile erection Sohn et al. 2008
Penile expression level of eNOS/nNOS increased
Rats, treated with high
dose corticosterone
Increase CE and ejaculation frequency Lau et al. 2012
Decrease ejaculation latency
Reverse suppressed neurogenesis in subventricular zone and hippocampus
Rats, hemicastrated Improve CE of hemicastracted rats Luo et al. 2006
Mouse testicular tissue
Prevent the structural damage of the seminiferous tubules
Prevent DNA damage caused by H2O2
Mouse seminiferous
Reduced structural damage of seminiferous epithelium Wang et al. 2002
More motile spermatozoa
Rats, with BPA
Prevent detrimental effect caused by BPA in terms of: increase the weight of
testis and epididymis
Zhang et al. 2013
Prevent the decrease in the level of sex hormones
Decreased level of proapototic marker, e.g., Bax
Suppressed oxidative stress
Rats, treated with
Prevents detrimental effect caused by DOX in terms of: preserved weight of the
testis and epididymis—prevent degenerative changes (depletion of germ cells,
irregular seminiferous tubules and scarcity of spermatogonia)
Xin et al. 2012
Prevent the decline of sperm quantity and quality
Attenuate the increased oxidative stress in testicular tissues
Table 8.1  Summary of the prosexual effect of Wolfberry
8 Prosexual Effects of Lycium Barbarum
Sex of
Species or experimen-
tal model
Effect of wolfberry component Reference
Rats, exposed to ion-
izing radiation
Prevents detrimental effect caused by ionizing radiation in terms of: repair the
damage in testicular cells
Luo et al. 2011
Regulate the serum testosterone level and
Maintain histological integrity of testicular tissue
May have antiapoptotic effects against irradiation Luo et al. 2014
Female♀ Mating Rabbits Induce ovulation after injection of crude extract of wolfberry Suzuki et al. 1972
Mating Frozen porcine oocyte Increase maturation rate Huang et al. 2008
Gestation Human, single case A woman with premature ovarian failure and secondary amenorrhea had ovu-
lation returned and conceived after 3 months of therapy
Chao et al. 2003
Offspring Rats, offspring Reduce the impairment in memory and learning deficiency of female offspring
which were subjected to prenatal stress
Feng et al. 2010
Mating Normal human Drinking wolfberry juice showed Amagase and
Nance 2008, 2009
Subjective report of increased sexual ability and activity
Elevated level of in vivo antioxidant markers including SOD and GSH-Px in
eNOS endothelial nitric oxide synthase, nNOS neuronal nitric oxide synthase, CE copulatory efficiency, DNA deoxyribonucleic acid, B PA bisphenol A, DOX
doxorubicin, SOD superoxide dismutase, GSH-Px glutathione peroxidase
Table 8.1  (continued)
122 B. W.-M. Lau et al.
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Complement Altern Med. 2013;2013:690808. doi:10.1155/2013/690808.
... Considering that the impairment of neurogenesis is linked to different psychiatric disorders (Schoenfeld and Cameron, 2015), our results suggest that neurogenesis suppression is associated with DXM-induced depression-like and social anxiety-like behavior and restoration of the neurogenesis could be an alternative therapeutic target for theemotional distress. As Lycium barbarum polysaccharide (LBP) has shown neuroprotective properties (Lau et al., 2015) and it exerts neurogenesis-promoting effects (Lau et al., 2012), the present study evaluated the effect of LBP on DXM-treated rats. The results showed that LBP alleviated the DXM-induced depression-like and social anxiety-like behaviors and reverted the DXM-suppressed neurogenesis. ...
Dextromethorphan (DXM) is one of the common drugs abused by adolescents. It is the active ingredient found in cough medicine which is used for suppressing cough. High dosage of DXM can induce euphoria, dissociative effects and even hallucinations. Chronic use of DXM may also lead to depressive-related symptoms. Lycium barbarum, commonly known as wolfberry, has been used as a traditional Chinese medicine for the treatment of ageing-related neurodegenerative diseases. A recent study has shown the potential beneficial effect of Lycium barbarum to reduce depression-like behavior. In the present study, we investigated the role of Lycium barbarum polysaccharide (LBP) to alleviate DXM-induced emotional distress. Sprague Dawley rats were divided into 4 groups (n = 6 per group), including the normal control (vehicles only), DXM-treated group (40 mg/kg DXM), LBP-treated group (1 mg/kg LBP) and DXM+ LBP-treated group (40 mg/kg DXM and 1 mg/kg LBP). After two-week treatment, the DXM-treated group showed increased depression-like and social anxiety-like behaviors in the forced swim test and social interaction test respectively. On the other hand, the adverse behavioral effects induced by DXM were reduced by LBP treatment. Histological results showed that LBP treatment alone did not promote hippocampal neurogenesis when compared to the normal control, but LBP could lessen the suppression of hippocampal neurogenesis induced by DXM. The findings provide insights for the potential use of wolfberry as an adjunct treatment option for alleviating mood disturbances during rehabilitation of cough syrup abusers.
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To observe the effects of Lycium barbarum polysaccharides (LBP) on testis spermatogenic injuries induced by Bisphenol A (BPA) in mice. BPA was subcutaneously injected into mice at a dose of 20 mg/kg body weight (BW) for 7 consecutive days. LBP was administered simultaneously with BPA by gavage daily at the dose of 50, 100, and 200 mg/kg BW for 7 days. After treatment, the weight and the histopathology changes of testis and epididymis were examined; the contents of T, LH, GnRH, antioxidant enzyme, and malondialdehyde (MDA) in serum were detected; proapoptotic protein Bax and antiapoptotic protein Bcl-2 were also detected by immunohistochemical method. Results showed that the weights of testis and epididymis were all increased after supplement with different dosages of LBP compared with BPA group, and the activities of SOD and GSH-Px were significantly increased in LBP groups, while MDA contents were gradually decreased. Moreover, the levels of T, LH, and GnRH were significantly elevated in serum treated with 100 mg/kg LBP. LBP also shows significant positive effects on the expression of Bcl-2/Bax in BPA treated mice. It is concluded that LBP may be one of the potential ingredients protecting the adult male animals from BPA induced reproductive damage.
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The paper presents a highly selective analysis method for the determination of bisphenol A (BPA) in canned fish. The procedure consists of sample clean-up by sol–gel immunoaffinity chromatography followed by high performance liquid chromatography and fluorescence detection. BPA concentrations were determined in nineteen tuna, sardine and mackerel cans by analysing the solid and the liquid parts of the contents separately. In different tested matrices limits of detection (S/N=3) ranged from 0.2ng/g (sardines) to 1.8ng/ml (oil) and limits of quantification (S/N=6) from 0.4ng/g to 3.8ng/ml, respectively. In the solid part (fish) very low BPA levels (2–4ng/g) were found in mackerels, the highest level (59ng/g) in tuna. In oil significantly higher BPA concentrations were found than in brine. In all samples BPA concentrations were significantly lower than the Specific Migration Level of 0.6mg/kg for BPA migration into food established by the EU Commission in 2004.
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Lycium barbarum, commonly known as wolfberry, has been used as a traditional Chinese medicine for the treatment of infertility and sexual dysfunction. However, there is still a scarcity of experimental evidence to support the pro-sexual effect of wolfberry. The aim of this study is to determine the effect of Lycium barbarum polysaccharides (LBP) on male sexual behavior of rats. Here we report that oral feeding of LBP for 21 days significantly improved the male copulatory performance including increase of copulatory efficiency, increase of ejaculation frequency and shortening of ejaculation latency. Furthermore, sexual inhibition caused by chronic corticosterone was prevented by LBP. Simultaneously, corticosterone suppressed neurogenesis in subventricular zone and hippocampus in adult rats, which could be reversed by LBP. The neurogenic effect of LBP was also shown in vitro. Significant correlation was found between neurogenesis and sexual performance, suggesting that the newborn neurons are associated with reproductive successfulness. Blocking neurogenesis in male rats abolished the pro-sexual effect of LBP. Taken together, these results demonstrate the pro-sexual effect of LBP on normal and sexually-inhibited rats, and LBP may modulate sexual behavior by regulating neurogenesis.
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Male reproductive health has deteriorated considerably in the last few decades. Nutritional, socioeconomic, lifestyle and environmental factors (among others) have been attributed to compromising male reproductive health. In recent years, a large volume of evidence has accumulated that suggests that the trend of decreasing male fertility (in terms of sperm count, quality and other changes in male reproductive health) might be due to exposure to environmental toxicants. These environmental contaminants can mimic natural oestrogens and target testicular spermatogenesis, steroidogenesis, and the function of both Sertoli and Leydig cells. Most environmental toxicants have been shown to induce reactive oxygen species, thereby causing a state of oxidative stress in various compartments of the testes. However, the molecular mechanism(s) of action of the environmental toxicants on the testis have yet to be elucidated. This review discusses the effects of some of the more commonly used environmental contaminants on testicular function through the induction of oxidative stress and apoptosis.
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Adult neurogenesis has been a focus within the past few years because it is a newly recognized form of neuroplasticity that may play significant roles in behaviors and recovery process after disease. Mammalian adult neurogenesis could be found in two brain regions: hippocampus and subventricular zone (SVZ). While it is well established that hippocampal neurogenesis participates in memory formation and anxiety, the physiological function of SVZ neurogenesis is still under intense investigation. Recent studies disclose that SVZ neurogenesis is under regulation of reproductive cues like pheromones. Reciprocally, the newborn neurons may exert their effect on reproductive and maternal behaviors. This review discusses recent understanding of the interrelationship between neurogenesis and reproduction. The studies highlighted in this review illustrate the potential importance of neurogenesis in reproductive function and will provide new insights for the significance of adult neurogenesis.
Ethnopharmacological relevance: Lycium barbarum, a Solanaceous defoliated shrubbery, has been used as a kind of traditional Chinese herbal medicines for thousands of years. Lycium barbarum polysaccharide (LBP) is the main bioactive component of Lycium barbarum. The aim of this study was to investigate the radioresistant effect of LBP on the damage of male rats' reproductive system and spermatogenic cells caused by low-dose (60)Co-γ irradiation. Materials and methods: Male rats were randomly divided into 7 groups and treated with irradiation and/or LBP: normal control group, irradiation control group 1, irradiation control group 2, irradiation control group 3, LBP + irradiation group 1, LBP + irradiation group 2, and LBP + irradiation group 3. Results: It is found that mating function and testis organ coefficient in LBP + irradiation groups were significantly better than that of the corresponding irradiation control groups. LBP significantly up-regulates the expression of Bcl-2 while down-regulating the expression of Bax. And LBP also plays an important role in prevention mitochondrial membrane potential decrease. In addition, LBP can significantly reduce spermatogenic cells apoptosis. Conclusion: LBP has obvious protective effect on the male rats' reproductive function and spermatogenic dysfunction induced by irradiation.
The reproductive performance of male livestock is of economic importance, and improving semen quantity and quality, especially for artificial insemination, additionally helps to avoid the loss of valuable genotypes. The review focuses on the impact of oxidative stress on sperm production and quality in livestock, and the potential role of plant based anti-oxidants to control this impact. From scientific reports dealing with livestock, the paper compiles evidence on effective dietary measures affecting sperm production and quality. Where little or no data are available on livestock, it refers to sources regarding other mammals, including man. The review concentrates on the use of distinct plants as feed supplements rather than on ways to treat deficiencies and imbalances in energy or macro- and micronutrients. Feeding of maca (Lepidium meyenii) and khat (Catha edulis) has been shown to positively affect sperm production and quality in animals. Some evidence points to favourable effects of leucaena (Leucaena leucocephala and Leucaena pallida), sesbania (Sesbania sesban), pomegranate (Punica granatum), tomato (Solanum lycopersicum) and amaranth (Amaranthus hypochondriacus) as well, but studies are either superficial or results are partially contradictory. Finally, the review considers the potential usefulness of medicinal herbs. The list of such plants includes Chinese herbs such as Lycium barbarum, Astralagus membranaceus, Acanthopanacis senticosi, Magnolia officinalis, Cornus officinalis and Psoralea corylifolia and the Indonesian plant Eurycoma longifolia. European candidate plants are Tribulus terrestris and Pendulum murex. Future research should include the screening of other plants, concentrating on the large number of plants rich in metabolites because of their presumed effectiveness. The modes of action often require clarification for the plants with demonstrated effects.
The present study aimed to investigate whether Lycium barbarum polysaccharides (LBP) would protect against doxorubicin (DOX)-induced testicular toxicity. Male Sprague-Dawley rats were treated with distilled water (4 mL/kg) or LBP (200 mg/kg, p.o.) daily for 10 days and followed by saline (0.9 %, 10 mL/kg) or DOX (10 mg/kg) intravenous injection at day 7. Pretreatment with LBP ameliorated DOX-induced reduction in the testicular weights, sperm concentrations and percentage of motile sperms, as well as the increase in abnormal sperm rate. LBP administration to DOX-treated rats successfully reversed the changes in MDA and GHS-Px levels. Compared with the control, pretreatment with LBP significantly increased the plasma testosterone level in the LBP + DOX group. The histopathology examinations further confirmed that LBP effectively attenuated DOX-induced severe degenerative changes of seminiferous tubules. This study illustrated the capability of LBP in attenuating testicular oxidative stress and protecting testis-specific toxicity in DOX-exposed rats.
To investigate the effects of bisphenol A (BPA) on embryo and uterine factors in embryo implantation, timed pregnant C57BL6 females were treated subcutaneously with 0, 0.025, 0.5, 10, 40, and 100mg/kg/day BPA from gestation days 0.5-3.5. In 100mg/kg/day BPA-treated females, no implantation sites were detected on day 4.5 but retention of embryos in the oviduct and delayed embryo development were detected on day 3.5. When untreated healthy embryos were transferred to pseudopregnant females treated with 100mg/kg/day BPA, no implantation sites were detected on day 4.5. In 40 mg/kg/day BPA-treated females, delayed implantation and increased perinatal lethality of their offspring were observed. Implantation seemed normal in the rest BPA-treated groups or the female offspring from 40 mg/kg/day BPA-treated group. These data demonstrate the adverse effects of high doses of BPA on processes critical for embryo implantation: embryo transport, preimplantation embryo development, and establishment of uterine receptivity.
Lycium barbarum, a famous Chinese medicinal herb, has a long history of use in traditional medicine as an antioxidant and to promote sexual fertility. Polysaccharides are the most important functional constituents in L. barbarum fruits. In this study, male rats were exposed to subchronic (60)Co-γ irradiation to investigate the effects of LBP on sperm quantity and motility, sexual ability, serum hormone levels, oxidative status and testicular tissue DNA damage on days 1, 7 and 14 of treatment. It was found that LBP significantly increased the sperm quantity and motility, shortened the erection, capture and ejaculation latencies, increased the number of captures and ejaculations, and improved the sexual ability of male rats. LBP also played a significant role in the recovery of serum testosterone levels, increased superoxide dismutase activity, decreased malondialdehyde levels, promoted oxidative balance and rescued testicular DNA damage. In conclusion, LBP has significant protective effects against damage induced by local subchronic exposure to (60)Co-γ irradiation, allowing rats to achieve near-complete recovery with LBP treatment.