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Advances in Nanoparticles, 2015, 4, 98-106
Published Online November 2015 in SciRes. http://www.scirp.org/journal/anp
http://dx.doi.org/10.4236/anp.2015.44011
How to cite this paper: Hien, N.Q., Van Phu, D., Duy, N.N., Quoc, L.A., Lan, N.T.K., Quy, H.T.D., Van, H.T.H., Diem, P.H.N.
and Hoa, T.T. (2015) Influence of Chitosan Binder on the Adhesion of Silver Nanoparticles on Cotton Fabric and Evaluation
of Antibacterial Activity. Advances in Nanoparticles, 4, 98-106. http://dx.doi.org/10.4236/anp.2015.44011
Influence of Chitosan Binder on the
Adhesion of Silver Nanoparticles on
Cotton Fabric and Evaluation of
Antibacterial Activity
Nguyen Quoc Hien1*, Dang Van Phu1, Nguyen Ngoc Duy1, Le Anh Quoc1, Nguyen T. Kim Lan1,
Hoang T. Dong Quy2, Huynh T. Hong Van2, Phan Ha Nu Diem3, Tran Thai Hoa3
1Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, Ho Chi Minh
City, Vietnam
2University of Science, Vietnam National University in Ho Chi Minh City, Ho Chi Minh City, Vietnam
3College of Science, Hue University, Hue City, Vietnam
Received 22 September 2015; accepted 6 November 2015; published 9 November 2015
Copyright © 2015 by authors and Scientific Research Publishing Inc.
This work is licensed under the Creative Commons Attribution International License (CC BY).
http://creativecommons.org/licenses/by/4.0/
Abstract
Colloidal silver nanoparticles (AgNPs) with particle size less than 10 nm and concentration of 2
mM/L (~200 mg/L) were synthesized by gamma Co-60 ray irradiation of Ag+/chitosan solutions
with different chitosan concentration of 0.5%, 1% and 2% (w/v). Incorporation of AgNPs onto
cotton fabric was carried out by padding method with 100% wet pick-up. The content of AgNPs
deposited on cotton fabric and released from cotton fabric after repeated washing was deter-
mined by inductively couple plasma-atomic emission spectroscopy (ICP-AES). The results indi-
cated that cotton/AgNPs fabric made from padding into AgNPs solution with 0.5% - 1% chitosan
was the best one of AgNPs adhesion ability on cotton fabric. Results on antibacterial activity
against S. aureus showed that cotton/AgNPs fabric with AgNPs content more than 100 mg/kg ex-
hibited highly antibacterial activity (η > 98%). The mechanical property (tensile strength and
elongation) of cotton/AgNPs fabrics was almost unchanged in comparison with untreated cotton
fabric. Thus, the resultant cotton/AgNPs fabric with highly antibacterial activity can be potentially
used as bed drapes and/or patient uniforms in hospitals, etc.
Keywords
Gamma Co-60 Ray, Silver Nanoparticles, Chitosan, Cotton Fabric, Antibacterial
*Corresponding author.
N. Q. Hien et al.
99
1. Introduction
The incorporation of silver nanoparticles (AgNPs) into polymers and/or inorganic carriers is of great interest for
many researchers because of the potential applications of these nanocomposite materials in medicine [1]-[5],
photocatalysis [6] [7], water treatment [8]-[12], textile [13]-[18], etc. More details of the immobilization and
practical applications of antibacterial nanoparticles on different carriers can be referred to the paper reviewed
recently by Moritz and Geszke-Moritz [19]. It is well known that AgNPs have a broad antibacterial activity
while exhibiting low toxicity towards mammalian cells at bacterial killing doses [20] [21]. Nanotechnology has
facilitated the production of smaller size of AgNPs with the increase of large surface area-to-volume ratios. It is
generally accepted that the smaller the AgNPs size, the stronger the antimicrobial activity [22] [23].
Various methods for the synthesis of AgNPs based on bottom-up approach, i.e. reduction of Ag+ ions to Ago
(zero-valent silver) in solution have been reported [24] [25] and the most common method is chemical reduction
of silver salt precursor using chemical reducing agents [19]. In comparison with other methods, gamma Co-60
ray irradiation has been considered as an effective method with several advantages as described in our previous
paper [24]. Chitosan is a natural polysaccharide derived from the deacetylation of chitin with both antibacterial
property and biocompatibility [26]. Chitosan has been used as stabilizer in the synthesis of AgNPs by
γ
-irradia-
tion method [25] [27] [28]. AgNPs were stabilized by chitosan through simultaneously steric and electrostatic
effect by interaction of -OH and -NH2 groups on the surface of AgNPs. Furthermore, chitosan and AgNPs acted
synergistically against bacteria and as a result the AgNPs/chitosan exhibited higher antibacterial activity than
any component acting alone [28]. On the other hand, chemical interactions between chitosan and cellulose were
reported in chitosan-treated cellulose by diffuse reflectance spectroscopic techniques (UV-Vis and FTIR) [29].
Therefore, chitosan is considered as a suitable binder for the adhesive enhancement of AgNPs with cotton fabric
and as an inducer for the synergistic antibacterial activity together with AgNPs for AgNPs/chitosan treated cot-
ton fabric.
In the present study, AgNPs were synthesized by
γ
-irradiation using chitosan as both stabilizer and hydroxyl
free radical scavenger and the as-synthesized AgNPs stabilized by chitosan were incorporated onto cotton fabric.
The influence of chitosan binder on the adhesion of AgNPs on cotton fabric after repeated washing, the antimi-
crobial activity against Staphylococcus aureus (S. aureus) and the mechanical property of the as-prepared AgNPs/
cotton fabrics were also investigated.
2. Experimental
2.1. Materials
Analytical grade AgNO3 and lactic acid were purchased from Shanghai Chemical Reagent Co., China. Chitosan
made from shrimp shell with deacetylation degree of about 90% and Mw of 9.2 × 104 g/mol was supplied by
Chitosan Co., Vung tau, Vietnam. Bacterium strain namely S. aureus ATCC 6538 was provided by University
of Medicine-Pharmacy, Ho Chi Minh City. The Mueller Hinton agar medium for bacterial incubation was pur-
chased from Himedia, Mumbai, India. Distilled water was used in all experiments. Cotton fabric weighting 120
g/m2 was provided by VICOTEX Company, Vietnam.
2.2. Methods
2.2.1. Synthesis of AgNPs/Chitosan by
γ
-Irradiation
Stock chitosan solution (2%, w/v) was prepared by dissolving 2 g chitosan in 100 mL lactic acid solution 1%
(v/v) and stored overnight. Chitosan solution was filtered through stainless steel net (200 mesh) to separate un-
dissolved solid. The desired content of AgNO3 was mixed with chitosan solution to prepare three solutions with
2 mM Ag+ and different chitosan concentration of 0.5%, 1% and 2% (w/v). And then, the prepared Ag+/chitosan
solutions were put into glass bottles with plastic caps. The irradiation of Ag+/chitosan solutions to prepare
AgNPs was carried out on a gamma Co-60 irradiator STSVCo-60/B (Hungary) at VINAGAMMA Center, Ho
Chi Minh City with absorbed dose of about 7 kGy [27] [30].
2.2.2. AgNPs Characterization
UV-Vis spectra of AgNPs/chitosan solutions which were diluted by water to 0.2 mM were recorded on an
UV-2401PC, Shimadzu, Japan and the size of AgNPs was calculated from TEM images taken on a JEM 1010,
N. Q. Hien et al.
100
JEOL, Japan [27].
2.2.3. Incorporation of AgNPs onto Cotton Fabric and Characterization
Before use, cotton fabric was washed to remove glue then dried and cut into equal-sized square pieces of 0.2 ×
0.2 m2. All cotton fabric samples were padded in AgNPs/chitosan solutions of about 5 min. and then squeezed to
wet pick-up of 100%. Afterwards, AgNPs treated cotton fabrics (AgNPs/cotton fabric) were air dried under am-
bient conditions. The silver content in AgNPs/cotton fabric samples was determined by inductively coupled plasma-
atomic emission spectroscopy (ICP-AES) on a Perkin-Elmer, Optima 5300 DV. The mechanical property (ten-
sile strength, Fb and elongation at break,
ε
b) of AgNPs/cotton fabrics was measured on a Tensile tester
Zwick/Roell, Germany following an ASTM method D 5035.
2.2.4. Washing and Silver Release from AgNPs Incorporated Cotton Fabrics
The washing process of AgNPs/cotton fabrics was carried out by the process as described by El-Rafie et al. with
5, 10 and 20 washings [17]. The silver content in AgNPs/cotton fabric after washing was also determined by
ICP-AES method.
2.2.5. Antibacterial Activity Tests
The antibacterial activity of cotton/AgNPs fabrics was tested against S. aureus by using a shaking flask as de-
scribed by Zhang et al. [16] with some modifications. Briefly, 1 g sample fabric, cut into small pieces with a
size of about 0.25 × 0.25 cm2 was dipped into a flask containing 100 ml of S. aureus suspension with a cell con-
centration of about 106 CFU/ml. The flask was then shaken at 150 rpm on a rotary shaker at room temperature
for 24 h. Afterwards, the number of bacteria forming units (CFU) in each mixture sample was quantified by
spread plate on Mueller Hinton agar plates and the antibacterial efficiency, η(%) was calculated as follows [16]
[27]:
()
( )
oio
% 100 N N N
η
−= ×
where No and Ni were the CFU/ml from the original cotton fabric and the AgNPs/cotton fabric, respectively.
3. Results and Discussion
3.1. AgNPs/Chitosan Characterization
The chitosan stabilized AgNPs solutions with suitably appropriate concentration are protected from aggregation
due to both steric and electrostatic stabilization effect of chitosan which has abandon of −OH and −NH2 groups
along the molecular chains [25] [31]. Therefore, colloidal AgNPs/chitosan solution is fairly stable during storage
time at ambient temperature [27]. The schematic capping mechanism of AgNPs by chitosan has been proposed
by Huang et al. [25]. However, they used rather high Ag+ concentration (~40 mM), therefore it caused gelation
of Ag+ with chitosan during preparation of Ag+/chitosan solution. In our preparation of 2 mM Ag+ in 0.5% - 2%
chitosan solution, no gelation occurred. The synthesis of AgNPs by gamma Co-60 irradiation method, chitosan
has been used as a stabilizing and free radial •OH scavenging agent [18] [25] [27]. During irradiation Ag+ ion is
reduced to Ago atom by
aq
e
−
and H• and Ago atoms will be agglomerated to form AgNPs that are capped by
chitosan or by other stabilizers. The detail reducing mechanism of formation of AgNPs by gamma Co-60 irradi-
ation method can be referred to the papers reported by Du et al. [24] and Huang et al. [25]. The UV-Vis spectra
of 2 mM AgNPs stabilized by 0.5%, 1% and 2% chitosan and TEM images with particle size distribution were
shown in Figure 1 and Figure 2, respectively. Table 1 summarized the value of optical density (OD), maximum
absorption wavelength (
λ
max) and average diameter (d) of AgNPs synthesized in different chitosan concentra-
tions.
The obtained results indicated that the AgNPs diameter for three chitosan (CTS) concentrations namely 0.5, 1
and 2% was not much different from each other in the range of ~7 - 9 nm. The reason may be due to chitosan
was used for stabilization of 2 mM AgNPs with high concentration and already reached to the critical concentra-
tion of stabilizer for protecting AgNPs to form the smallest particles size. Du et al. already reported the critical
concentration of polyvinyl alcohol for preparation of the smallest size (~10 nm) of 20 mM AgNPs by gamma
Co-60 irradiation was of 2% - 4% [32].
N. Q. Hien et al.
101
Figure 1. UV-Vis spectra of colloidal AgNPs solutions stabilized with (a)
0.5, (b) 1 and (c) 2% chitosan.
Figure 2. TEM images and particle size distribution histograms of AgNPs stabilized in CTS: 0.5 (A,a), 1 (B,b) and 2% (C,c).
Table 1. Value of OD, λmax and diameter of AgNPs prepared in different chitosan concentrations.
Sample OD λmax (nm) d (nm)
AgNPs 2 mM/CTS 0.5% 1.70 408 8.8 ± 0.8
AgNPs 2 mM/CTS 1% 2.09 406 7.1 ± 0.3
AgNPs 2 mM/CTS 2% 2.58 405 6.8 ± 0.5
N. Q. Hien et al.
102
3.2. Silver Release from AgNPs/Cotton Fabric by Washing
Figure 3 showed the results of silver release from AgNPs/cotton fabric by washings. The obtained results indi-
cated that the suitable concentration of chitosan for better adhesion of AgNPs on cotton fabric was of 0.5% - 1%.
The content of silver release from AgNPs stabilized by 0.5 and 1% chitosan after 20 washing cycles was of ~30%
compared to that of ~44% for 2% chitosan sample. It indicated that the higher the binding concentration did not
result better adhesion of AgNPs on cotton fabric, particularly in case of chitosan. The reason may be due to ex-
cessive chitosan content that could not strongly adhere to cotton fibers, therefore during washing, the excessive
portion of chitosan will be easily released and taken AgNPs together with. Further study should be carried out to
clarify this phenomenon.
3.3. Antibacterial Efficiency of AgNPs Coated Cotton Fabrics
The antibacterial efficiency of AgNPs/cotton fabrics with different content of AgNPs against S. aureus was pre-
sented in Table 2 and Figure 4. It can be observed that all the AgNPs/cotton fabrics with silver content from
124 mg/kg to 245 mg/kg fabric showed highly antibacterial efficiency (>98% compared with untreated cotton
fabric). The antibacterial efficiency increased slightly from 98.04% to 99.98% with the increase of the silver
content of AgNPs/cotton fabric. According to the results reported by Zhang et al. [16], the antimicrobial effi-
ciency of AgNPs coated cotton fabric with silver content of about more than 158 mg/kg fabric against S. aureus
was almost to reach to
η
≈ 100%. The reason of the difference of antibacterial efficiency of Zhang et al. [16]
and our result in this work may be due to the cell concentration of S. aureus that they used for antibacterial test
was of about 106 CFU/ml which was smaller compared with 107 CFU/ml in our antibacterial test experiment.
The results also indicated that after 20 washing cycles, AgNPs/cotton fabric still maintained highly antibacterial
activity.
Figure 3. Silver content of AgNPs/cotton fabrics after washing.
Table 2. Silver content and antibacterial efficiency against S. aureus of AgNPs/cotton fabrics.
Cotton fabric samples Silver content (mg/kg fabric) Surviving cells, (CFU/ml) Efficiency (η, %)
Untreated 0(*) 1.13 × 107 -
1 245 2.70 × 103 99.98
2 204 1.25 × 104 99.89
3 177 1.35 × 105 98.81
4 124 2.21 × 105 98.04
(*) not detected by ICP-AES (untreated cotton fabric).
N. Q. Hien et al.
103
Figure 4. S. aureus colonies forming on agar plates: (a) control (cotton fabric); (b), (c), (d)
and (e) AgNPs/cotton fabric with 245, 204, 177 and 124 mg silver/kg fabric, respectively.
Table 3. The value of Fb) and
ε
b of cotton and cotton/AgNPs fabrics.
Sample Fb, N
ε
b, %
Cotton fabric 299.4 ± 06.5 24.3 ± 1.4
AgNPs/cotton fabric (chitosan 0.5%) 289.1 ± 14.8 21.7 ± 2.9
AgNPs/cotton fabric (chitosan 1%) 289.9 ± 09.7 23.3 ± 1.5
AgNPs/cotton fabric (chitosan 2%) 270.4 ± 07.4 23.3 ± 2.8
In addition, according to our results reported in a previous paper, AgNPs/cotton fabrics are innoxious to skin
with coefficient of k = 0 [18]. Furthermore, concerning environmental impact of AgNPs, it is also worth to note
that the AgNPs in wastewater is almost completely transformed into Ag2S that has extremely low solubility and
exhibits a much lower toxicity than other forms of silver [33] [34]. Therefore, AgNPs release from AgNPs/cot-
ton fabric into wastewater by washing will be transformed into Ag2S that is considered to have no significant
impact to the environment [33]. Therefore, AgNPs/cotton fabric with highly antibacterial activity can be poten-
tially used as bed drapes and/or patient uniforms in hospitals, especially for patients with infectious diseases, etc.
3.4. Mechanical Property of AgNPs Coated Cotton Fabric
Table 3 presented the mechanical property particularly tensile strength (Fb) and elongation at break (
ε
b) of cot-
ton and AgNPs/cotton fabrics. As a result, Fb and
ε
b of AgNPs incorporated cotton fabrics were almost un-
changed in comparison with untreated cotton fabric.
4. Conclusion
Colloidal AgNPs solution was successfully synthesized by gamma Co-60 irradiation method using chitosan as
stabilizer and hydroxyl free radical scavenger. The diameter of 2 mM AgNPs stabilized with 0.5% - 2% chitosan
was of 7 - 9 nm. AgNPs stabilized with 0.5% - 1% chitosan were found to be better adhesion on cotton fabric.
Thus, the as-prepared AgNPs/cotton fabric with highly antibacterial activity, low content of silver release by
washing and safety can be potentially used as bed drapes and/or patient uniforms in hospitals, etc. Pilot scale
production line with 30 - 50 m2/h of AgNPs/cotton fabric by padding method has been carrying out.
Acknowledgements
The authors are thankful to VINAGAMMA Center, VINATOM for favorable conditions to perform this re-
search. We are also grateful to the contribution of Quy, H.T.D., Van, H.T.H., Diem, P.H.N. and Hoa, T.T. in
characterization of materials.
N. Q. Hien et al.
104
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