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Journal of Food Resource Science, 2015
ISSN 2224-3550 / DOI: 10.3923/jfrs.2015.
© 2015 Asian Network for Scientific Information
Production and Quality Evaluation of Green and Black Herbal Teas
from Moringa oleifera Leaf
Gabriel Ifeanyi Okafor and Nkemakonam Maryann Ogbobe
Department of Food Science and Technology, University of Nigeria, Nsukka, Enugu State, Nigeria
Corresponding Author: Gabriel Ifeanyi Okafor, Department of Food Science and Technology, University of Nigeria, Nsukka,
Enugu State, Nigeria Tel: +234-8023423908
ABSTRACT
Freshly plucked Moringa oleifera leaves were processed into green and black herbal teas, due
to its nutritional, pharmacological and medicinal properties. The unit operations were plucking,
destalking and steaming for green tea or withering, grinding, fermentation for black tea prior to
drying, milling, grading and packing. The tea samples were subjected to chemical and microbial
load analyses, using standard methods. The results revealed the following ranges: proximate
parameters; crude protein (7.25-26.62%), moisture content (10.37-70.48%), crude fat (1.82-2.74%),
crude fibre (1.09-19.37%), ash content (0.94-9.75%), carbohydrate (15.31-44.93%); phytochemicals;
saponins (2.25-5.50%), flavonoid (6.0-16.50%), steroid (1.05-5.05%) and terpenoid (0.70-1.23%) and
anti-nutritional factors; tannin (0.23-1.32%), phytate (5.5-18.75%), cyanide (2.55-12.00%) and
alkaloid (0.65-2.20%). The ranges of vitamins detected were; vitamins A (7-35-20.37 mg),
B1 (0.11-2.85 mg), B2 (0.07-21.46 mg) and C (8.25-213 mg), while minerals included calcium
(465.5-2057.5 mg), phosphorus (74.80-225.00 mg) and iron (8.23-32.15 mg). The microbial profile
of green tea revealed 8.34×102 CFU gG1 total viable count, whereas no mould growth was observed
in both green and black Moringa tea leaves.
Key words: Steaming, fermentation, proximate, micronutrients, phytochemicals, antinutrients
INTRODUCTION
Tea is the processed leaves of Camellia sinensis which upon infusion with cold or hot water,
gives a non-alcoholic beverage (Sharma et al., 2005). Tea consumption is a very ancient habit, with
legends from China and India indicating that it was initiated about five thousand years ago
(Dufresne and Franworth, 2001). Traditionally, tea was consumed to improve blood flow, eliminate
toxins and improve resistance to diseases (Balentine et al.,1997). According to Hertog et al. (1993),
numerous epidemiological studies linked tea consumption to reduction of the risk of cardio vascular
diseases, high cholesterol levels, diabetes (Vinson et al., 2001), arthritis (Haqqi et al., 1999),
osteoporosis (Hegarty et al., 2000) and dental carries. Polyphenols are the most biologically active
group of tea components (Park et al., 2012), which possesses antioxidative, antimutagenic and
anticarcinogenic effects (Yao et al., 2004). Valerga et al. (2012) modified polyphenol content and
composition of yerba mate as well as the capacity of its extracts to inhibit the oxidation of
β-carotene/linoleic acid system, after industrial processing. These beneficial effects of tea have been
attributed to the antioxidant properties of polyphenolic compounds, particularly the catechin
derivatives (Horzic et al., 2009). According to Gill et al. (2011), factors that determine the quality
of m ade tea in c lude the p l ucking m e t hod, fermentation time, sorting and particle size among
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J. Food Resour. Sci., 2015
others. Horzic et al. (2009) reported that four types of teas are produced; white tea, green tea
(both unfermented), oolong tea (semi-fermented) and black tea (fully fermented). Other classes of
teas include puerrh tea, scented teas, flavoured teas and herbal teas.
Herbal tea also known as tisane, or herbal infusion is simply the combination of boiling water
and dried leaves, flowers, fruits or herbs, often consumed for their physical or medicinal effects
(Kara, 2009). Herbal teas can be made with fresh or dried flowers, leaves, seeds or roots, generally
known to posses medicinal values by pouring boiling water over the plant parts and letting them
steep for a few minutes. In Nigeria, leaves such as bitter leaf (Okafor et al., 2009), “Utazi” and
“Uziza” have been successfully used for production of herbal teas, due to their medicinal properties.
Moringa oleifera has so many attributes such as the green nature of the leaves, the antioxidant
properties, polyphenol content, phytochemicals properties among others (Fahey, 2005), which
makes it similar to the leaves of Camellia sinensis and useful for tea manufacturing. They also
form the basis for the name “Herbal tea” often given to teas other than from the leaves of
Camellia sinensis, because they are associated with management of different ailments. The use of
herbal remedies especially in the form of teas for management of various diseases is gaining
increasing popularity, making them the main stay of health care system, especially among the
rural populace in the developing countries (Ogbonnia et al., 2011).
Nutrient enrichment with Moringa oleifera represents a superior option for mitigating the
effects of micronutrient deficiency in impoverished areas. According to De Saint Sauveur and Broin
(2010), Moringa leaf powder is highly concentrated with micronutrients, that a few grams a day
can help to combat vitamin, mineral and protein deficiencies. They equally reported that eating
100 g fresh Moringa oleifera leaves provide as much protein as an egg, as much calcium as a big
glass of milk, as much iron as a 200 g beef steak, as much vitamin A as a carrot and as much
vitamin C as an orange. Its leaves are used as nutritional supplement and growth promoters
(Sanchez-Machado et al., 2006), antiulcer, diuretic, anti-inflammatory agent and for enhancing
wound healing (Udupa et al., 1994; Pal et al., 1995). Prakash (1998) demonstrated that the aqueous
extract of the leaves has antifertility activity, while Tahiliani and Kar (2000) used it in regulating
adult Swiss rat’s thyroid hormone status. Moringa is not used as a stimulant because it contains
no caffeine. Different processing steps such as fermentation, steaming, among others help to
remove the anti-nutrients present in Moringa leaves. The aim of processing Moringa oleifera leaves
into green and black teas is to process them into convenient shelf stable forms and to preserve the
products, thus helping to increase food security, create employment opportunities and generate
income.
MATERIALS AND METHODS
Raw materials procurement: Fresh green leaves of Moringa oleifera were plucked from a garden
at Orba in Nsukka local government area, Enugu State, Nigeria.
Method of producing green tea from the leaf of Moringa oleifera: The flowchart for
production of green tea from Moringa oleifera leaves is presented in Fig. 1. Freshly plucked leaves
of Moringa oleifera weighing 1000 g were washed using pipe borne water. An electric steamer
(John Lewis model) was plugged to an electric socket and the water reservoir filled with cold water
until the maximum level was reached. The timer was set for 60 min count down. The steamer was
switched on and water was allowed to reach boiling point temperature of 100°C using inserted
thermometer. The washed leaves were weighed in batches of 92 g using a digital weighing balance
(Camry 302), placed in the plastic containers of the steamer and allowed to steam for 3 min. After
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J. Food Resour. Sci., 2015
Weighing
Was h ing
M
oringa leaves
Steaming (for 3 min at 100°C)
Cooling (for 30 min)
Rolling
Dryin g (6 h at 50 °C)
Rolling
Packaging
Plucking
Fig. 1: Production of green herbal tea from Moringa leaf
the steaming process, the leaves were allowed to cool at room temperature for 30 min prior to
rolling, using a manual plate mill (Corona) to disrupt the leaf cells and increase their surface area,
in order to enhance the drying rate. The rolled leaves were spread on trays and dried immediately
in a hot air oven for 6 h at 50°C till the product became crispy. After drying, the products were
coarsely milled using a manual plate corona mill to obtain a coarse product which was sieved using
a USA standard testing sieve No. 30 and packed in an air tight container and stored at room
temperature.
Method of producing black tea from the leaves of Moringa oleifera: The flowchart for
production of black tea from Moringa oleifera leaves is presented in Fig. 2. Moringa oleifera leaves
(1 kg) were washed with tap water and allowed to drain. The drained leaves were thinly spread on
jute bags and withered under shade for 3 h. The leaves were allowed to lose about 60% of their
moisture in order to make them pliable for rolling. This was done by weighing at intervals of
30 min. The withered leaves were rolled using a manual corona plate mill. Rolling was done to
disrupt the leaf cells and expose some of the essential oils and juice necessary for fermentation.
After rolling, the leaves were thinly spread on trays and left at room temperature for 3 h for
fermentation to take place. The colour of the leaves darkened as a result of fermentation/oxidation.
The fermented leaves were dried in a hot air oven at the temperature of 50°C for 4 h, cooled and
coarsely milled using a manual corona plate mill in order to obtain a coarse product, which was
sieved using a USA standard testing sieve No. 30 and packaged into an air tight container.
Determination of proximate composition: The proximate analysis (moisture, fat, ash, protein,
carbohydrate and fibre) of the fresh leaves and products was carried out using AOAC (2010)
method. However, carbohydrate content was determined by the difference in the sum of all other
proximate content from 100.
Percentage carbohydrate = 100-(Moisture (%)+Ash (%)+Protein (%)+Fibre(%)+fat (%))
Determination of phytochemical composition: The steroid content of the samples was carried
out using the method of Subhadhirasakul and Pechpongs (2005). Terpenoid was determined using
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J. Food Resour. Sci., 2015
Plucking
Weighing
Was h ing
Draining
Withering (for 6 h under the shade)
Rolling
Fermentation (3 h at 29-30°C)
Drying (for 4 h at 50°C)
Cooling
Packaging
Rolling
M
oringa oleifera
leaves
Fig. 2: Production of black herbal tea from Moringa oleifera leaf
the same procedure with steroid. The only difference was that methanol was used in terpenoid
determination instead of chloroform. Flavonoid was determined using the method of Boham
and Kocipai-Abyazan (1974) while, saponin was determined using method of Obadeni and Ochuko
(2002).
Determination of anti-nutrients: The anti-nutrients were determined as follows: phytate was
determined using method of Oberlase (1973). Tannin was determined using the method of Pearson
(1976). The gravimetric method of Harbone (1973) was adopted in determining the alkaloid content
of the samples. Cyanide content was determined using the method of Onwuka (2005).
Determination of mineral elements: Minerals occur in foods in both organic and inorganic
compounds of the nutrients essential for human nutrition. Some minerals such as calcium, iron and
phosphorous considered to be among the top mineral elements were analyzed as follows; the
phenanthroline method described by Pearson (1976) was used to determine the iron content of the
samples. Calcium was determined using the method described by Harbone (1973), while the
spectrophotometer molybdate method as described by Pearson (1976) was used to determine
phosphorous content.
Determination of vitamin composition: Provitamin A was determined using the method of
Pearson (1976). Vitamin C was determined using method of Barakat et al. (1973), while
determination of thiamin was done using the method described by Pearson (1976). Niacin content
of the sample was determined using AOAC (2010).
Microbial analysis: The total viable and mould counts were carried out on the Moringa leaf tea
samples. The total viable count of each sample was determined by pouring plate method using
nutrient agar as described by Prescott et al. (2005), while mould count was done according to
method of Prescott et al. (2005) on Potato Dextrose Agar (PDA).
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J. Food Resour. Sci., 2015
Statistical analysis: Data analysis was carried out using one-way analysis of variance (ANOVA)
based on completely randomized design and mean separation was by Duncan’s New Multiple Range
Test (DNMRT). Significance was accepted at p<0.05.
RESULTS AND DISCUSSION
Proximate composition of fresh and powdered Moringa oleifera leaves, black and green
teas: The proximate composition of the Moringa black tea, green tea, powdered and fresh leaves
is shown in Table 1. The moisture content of the samples, ranged from 8.76-70.48%. Powdered
Moringa leaves had the lowest moisture content of 8.76%, while fresh Moringa leaves had the
highest moisture content of 70.4%. The moisture content of green and black teas did not fall within
the suggested limit (below 6.5%) of Owuor (2003). The high moisture content of Moringa tea
samples could be attributed to improper packaging, making the samples to reabsorb moisture
from the humid environment, after processing. Controlling the moisture content of tea products
is an important factor in tea preservation, particularly for inhibition of microbial growth
(Heong et al., 2011). According to Mohammed and Sulaiman (2009), high moisture content aids
fungal growth. The ash content of a sample, which is an indication of its mineral content, does not
necessarily indicate high quality, except when there is a favourable balance of the essential
minerals (Ahmed et al., 1989). The ash content of these samples ranged from 4.04-9.75%, which
could be attributed to the different processing methods used for the teas. Moringa leaf powder was
found to have the lowest ash content (4.04%), while black tea had the highest ash content (9.75%).
These high ash contents suggest that the samples are good sources of minerals (Mohammed and
Suleiman, 2009). There were significant (p<0.05) differences between the crude fibre content of all
the samples. The powdered Moringa leaves had the highest crude fibre content (18.64%), which is
in agreement with the crude fiber content (18.82%) reported for Srilanka tea (Mohammed and
Sulaiman, 2009), while the fresh leaf had 1.09% crude fibre content. Crude fibre stimulates the
movement of the bowel and helps to prevent constipation (Okanlawon, 2000).
The fat content of the samples that ranged from 1.82-2.72% were significantly (p<0.05) different
from each other. Fresh Moringa leaf had the least fat content (1.82%), while green tea had highest
content followed by Moringa leaf powder and black tea. The disparity between the fat content of
the processed samples could be due to drying which concentrated nutrients, steaming or withering
processes that contributed to loss of moisture (Jabeen et al., 2015). The protein content of the
samples which ranged from 7.25-26.65% were significantly (p<0.05) different from each other, with
Moringa leaf powder being the highest (26.62%) followed by green and black Moringa teas, while
fresh Moringa leaf had the least protein content (7.25%) probably due to its high percentage of
moisture content. The carbohydrate content of the powdered Moringa leaf sample was highest
(26.62%), because most of its moisture content had been removed by drying. followed by green tea
(22.3%). Fresh Moringa leaf had the least carbohydrate content (7.25%) because it was still in its
fresh state when analysed and consequently contained more moisture.
Table 1: Proximate composition of Moringa leaf and tea samples (dry weight basis)
Samples Protein (g) Moisture (g) Fat (g) Crude fibre (g) Ash (g) CHO (g)
Fresh leaf 7.25±0.08d70.48±0.12a1.820±0.08c1.09±0.05d4.04±0.07c15.31±0.07d
Leaf powder 26.62±0.12a8.76±0.20d2.380±0.05b19.37±0.18a9.36±0.02b33.4900±0.02c
Green Tea 22.36±0.00b10.37±0.10c2.74±0.04a18.64±0.44b9.45±0.21ab 44.9000±0.16a
Black Tea 17.72±0.05c12.62±0.07b1.910±0.02c17.82±0.09c9.75±0.02a40.1700±0.18b
Values represents Mean±SD (n = 3). Mean values within each column followed by different superscript letter significantly (p<0.05) differ
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J. Food Resour. Sci., 2015
Vitamin composition of Moringa tea samples, fresh and powdered leaves: The vitamin
composition of Moringa black tea, green tea, fresh and powdered leaves is shown in
Table 2. Vitamins are organic compounds present in trace amounts in our diets (Heong et al., 2011).
Black Moringa leaf tea had a significantly higher (p<0.05) amount of vitamin A than other samples,
while fresh Moringa leaf sample had the least vitamin A content (7.5 β-carotene eq mgG1). This
could be attributed to the high percentage of moisture present in the leaves. In terms of vitamin
B1 (thiamine), black tea had high vitamin B1 content (2.85 mg) when compared to other samples.
Green Moringa tea had the lowest vitamin B1 content (0.11 mg). This could be attributed to the
steaming process given to the leaves, during processing. In terms of vitamin B2 (riboflavin) content,
black Moringa tea leaf had the highest values (20.80 mg), while Moringa fresh leaf had the lowest
riboflavin content (0.07 mg). There was no significant (p<0.05) difference between the vitamin B2
contents of Moringa black tea and powdered leaves. In terms of vitamin C (ascorbic acid) content,
the fresh Moringa leaf sample was significantly (p<0.05) higher than other samples. Moringa green
tea had the least ascorbic acid content (8.25 mg), due to the fact that vitamin C is heat labile and
subsequently, was drastically reduced during the steaming process.
Mineral composition of Moringa leaf tea samples, fresh and powdered leaves: The mineral
composition of Moringa black tea, green tea, fresh and powdered leaves is shown in Table 3. The
result revealed that black tea had the highest concentration of all the minerals compared to other
samples, which could be attributed to the high percentage of ash present. Black Moringa leaf tea
also had the highest ash content (Table 1), which is in agreement with the observation of
Mohammed and Sulaiman (2009) that high content of ash is an indication of a good source of
minerals. The production processes of black tea that involves withering which reduces moisture
content of fresh leaf to one third (Jabeen et al., 2015), helps to reduce loss of juice during
maceration that aids enzymatic oxidation process called fermentation (Hsu et al., 2011) and
subsequently enhanced mineral retention compared to green tea. There were significant (p<0.05)
differences in the content of minerals between the samples. Thus, black tea had the highest
calcium content (2057.50 mg) followed by green tea (2039 mg), due to concentration of minerals
during drying, while fresh Moringa leaves had the least calcium content (465.50 mg). Relative to
phosphorus and iron the same trend was observed, with Moringa black tea also having the highest
content of these minerals, while Moringa fresh leaves had the least content. Steam blanching of
Moringa leaves and subsequent maceration led to loss of fluid from the leaf matrix that contributed
to reduction in the mineral content green tea. These vitamins and minerals are very important for
a healthy diet and could therefore boost the immune system (Jimoh and Oladiji, 2005).
Table 2: Vitamin composition of Moringa leaf and tea samples (dry weight basis)
Samples Vitamin A (β eq mgG1) Vitamin B1 (mg) Vitamin B2 (mg) Vitamin C (mg)
Fresh leaf 7.35±0.21d0.20±0.02c0.07±0.00c213.00±4.24c
Leaf powder 18.70±0.14b2.68±0.04b20.80±0.12a16.55±0.07c
Green tea 8.12±0.17c0.11±0.01d9.60±0.56b8.25±0.07d
Black tea 20.37±0.03a2.85±0.02a21.46±0.04a76.40±2.68b
values represents Mean±SD (n = 3). Mean values within each column followed by different superscript letter significantly (p< 0.05) differ
Table 3: Mineral composition of Moringa leaves and tea samples (dry weight basis)
Samples Calcium (mg) Phosphorus (mg) Iron (mg)
Fresh leaf 465.50±7.77d74.80±0.28d8.23±0.04d
Leaf powder 1982.00±3.53c216.10±1.27c27.55±0.06c
Green tea 2039.00±0.00b220.00±0.00b30.30±0.56b
Black tea 2057.50±3.53a225.00±1.41a32.15±0.21a
Values represents Mean±SD (n = 3). Mea n values within each column followed by different superscript letter significantly (p<0.05) differ
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J. Food Resour. Sci., 2015
Phytochemical composition of Moringa leaf tea samples, fresh and powdered leaves: The
phytochemical composition of the Moringa black tea, green tea, powdered and fresh leaves is shown
in Table 4. According to Fahey (2005), phytochemicals are chemicals produced by plants and also
refer to only those chemicals which may have an impact on health, or on flavour, texture, smell or
colour of the plants but are not required by humans as essential nutrients. From the result,
significant (p<0.05) differences existed among all the samples. The powdered Moringa leaf sample
had the highest flavonoid content (16.50%), followed by green tea fresh Moringa leaf sample
which had the least flavonoid content (6.00%). Green Moringa tea contains higher flavonoid
content than black tea. Flavonoids, which are many in number, are strong anti-oxidants, also
found to be effective antimicrobial substances. They have been reported to possess substantial
anti-carcinogenic and anti-mutagenic activities due to their antioxidant and anti-inflammatory
properties (Havsteen, 2002). They are also active in reducing high blood pressure (Kasolo et al.,
2010). Saponin is the glucoside component referred to as “Natural detergent” because of their foamy
nature. The leaves of Moringa oleifera are utilized as soap by some communities in Uganda because
of the presence of saponins that form froth and act as soap (Kasolo et al., 2010). According to Davies
(1991), saponins have anti-carcinogenic properties, immune modulation activities and regulation
of cell proliferation as well as health benefits such as inhibition of the growth of cancer cells and
cholesterol lowering activity. Powdered Moringa leaves had a highly significant (p<0.05) saponin
content (5.50%), while green Moringa tea leaf had the lowest saponin content (2.25%). There was
no significant (p>0.05) difference between saponin content of green Moringa tea and that of fresh
leaf. For steroid, powdered Moringa leaf had the highest content of steroid (5.05%) that was
significantly (p<0.05) different from other samples. Black Moringa tea had a steroid content of
4.00%, while fresh Moringa leaf sample had the least steroid content (1.05%). The terpenoid content
powdered Moringa leaf had the highest terpenoid content, which could be attributed to shade
drying, as it was not given any other treatment. The terpenoid contents of all the samples were
generally low. However, there was no significant (p>0.05) difference between fresh Moringa leaf
and black Moringa tea samples, in terms of their terpenoid contents. According to Kasolo et al.
(2010), terpenoid and steroid present in M. oleifera leaves are active against bacteria such as
staphylococcus aureus and capable of preventing cancer.
Effect of processing on some anti-nutritional composition of Moringa oleifera tea
samples: The anti-nutritional composition of Moringa black tea, green tea, powdered and fresh
leaves is shown in Table 5. When any type of tea leaf is steeped in hot water it brews a “Tart”
Table 4: Phytochemical composition of Moringa leaf and tea samples (dry weight basis)
Samples Flavonoid (%) Saponin (%) Steroid (%) Terpenoid (%)
Fresh leaf 11.00±0.00b3.50±0.00b3.00±0.01c1.05±0.00b
Leaf powder 16.50±0.00a5.50±0.01a5.05±0.00a1.23±0.00a
Green tea 6.50±0.01c2.25±0.00c4.00±0.00b0.70±0.65c
Black tea 6.00±0.15d3.50±0.07b1.05±0.07d0.75±0.01c
Values represents Mean±SD (n = 3). Mean values within each column followed by different superscript letter significantly (p<0.05) differ
Table 5: Anti-nutritional composition of Moringa leaf and tea samples (dry weight basis)
Samples Tannin (%) Phytate (%) Cyanide (%) Alkaloid (%)
Powdered Moringa leaf 1.320±0.00a3.120±0.05a2.00±0.07a2.20±0.04a
Green Moringa tea 0.650±0.07b2.040±0.00c1.50±0.07c0.65±0.00c
Black Moringa tea 1.240±0.02a1.050±0.02b0.80±0.00b0.95±0.07b
Fresh Moringa leaf 0.227±0.01c0.950±0.02d0.55±0.02d1.00±0.00b
Values represents Mean±SD (n =3). Mean values within each column followed by different superscript letter significantly (p< 0.05) differ
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J. Food Resour. Sci., 2015
Table 6: Total viable count and mould count of Moringa leaf and tea samples
Sample Total viable count (CFU gG1) Mould count (CFU gG1)
Black Moringa oleifera leaf tea 1.34×102-
Powdered Moringa oleifera leaf 1.63×1025.0×10
Green Moringa oleifera leaf tea 8.0×101-
Fresh Moringa Oleifera leaf 2.02×102 2.1×10
-: not present, TVC: Total viable count
(astringent) flavor that is characteristic of tannins, which is due to the presence of catechins and
other flavonoids (Mohammed and Sulaiman, 2009). Powdered Moringa leaf and black Moringa tea
samples had the highest tannin contents, which were not significantly (p>0.05) different from each
other. Tannins are chemically distinct from other types of plant tannins such as tannic acid and
tea extracts, which have been reported to contain no tannic acid inhibit the absorption of minerals,
such as iron which may, if prolonged, lead to anaemia, if ingested in excessive quantities
(Mohammed and Sulaiman, 2009). Osada et al. (1997) recommended drinking tea and coffee
between meals, because foods rich in vitamin C are known to help in neutralizing tannin effects
on iron absorption. Adding lemon juice to tea will reduce the negative effect of tannins in iron
absorption as well (Mohammed and Sulaiman, 2009). However, adding milk to coffee and tea, has
very little or no influence on the inhibitory effect of tannin (Dommgang et al., 1998). Powdered
Moringa leaf sample had significantly (p<0.05) higher phytate content, when compared to other
samples, while fresh Moringa leaf sample had the least phytate content (0.95%). The cyanide
content of the samples ranged from 0.55-2.00% and was higher than the range 0.55-1.45% reported
by Omah and Okafor (2015) for cookies made with 5-20% addition of cassava cortex. Powdered
Moringa leaf sample had the highest cyanide content (2.00%) followed by green Moringa leaf tea
(1.5%), while fresh Moringa leaf sample had the lowest cyanide content (0.55%). Moringa oleifera
leaf sample also contain alkaloids which are nitrogen-containing naturally occurring compounds,
known to posses antimicrobial properties, due to their ability to intercalate with DNA of
microorganisms (Kasolo et al., 2010). The alkaloid content was high in powdered leaves (2.20%) and
least in green tea leaves (0.65%), which can be attributed to the steaming treatment given to the
leaves. No significant (p>0.05) difference was observed between Moringa black tea and fresh
Moringa leaf samples.
Microbial profile of Moringa leaf products: The microbial profile of Moringa black tea, green
tea, powdered and fresh leaves is shown in Table 6. Green Moringa leaf tea had the lowest total
viable count (8.0×101 CFU gG1) which may be attributed to the steaming treatment given to the
leaves. Fresh Moringa leaf sample had the highest TVC (2.02×102 CFU gG1). The high presence of
microorganisms in the sample may be attributed to environmental contamination. The black and
green Moringa teas did not have any mould growth in them, which may be because of the heat
treatment given to them. While, the mould growth of powdered and fresh Moringa leaves were
5.0×10 and 2.1×10 CFU gG1, respectively. The microbial levels detected in the teas were generally
low and would still be reduced during tea brewing with hot water.
CONCLUSION
The results provide empirical basis for production of high quality herbal teas from leaves of
plants other than that of Camellia sinensis. The moisture analysis conducted, clearly demonstrated
that green Moringa tea leaves had the lowest moisture content, which will enhance the shelf
stability of the products, if properly packaged. Green Moringa tea had high levels of protein and
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J. Food Resour. Sci., 2015
carbohydrate contents. The ash content of black Moringa tea leaf was highest when compared with
other samples, thus indicating it as a good source of minerals. The black Moringa tea had the
highest content of minerals when compared to other samples due to its high ash content. The
Moringa black tea sample had high vitamin C content but that of fresh leaves was highest. The
powdered Moringa leaves had the highest concentrations of phytochemicals, while Moringa teas
had significant amounts of these phytochemicals. The microbial result revealed that there were no
mould growths in black and green Moringa teas.
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