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The Potential Impact of Biofield Treatment on Human Brain Tumor Cells: A Time-Lapse Video Microscopy

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  • Lab Lifescience intelligentsia pvt. Ltd.

Abstract and Figures

Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non-GBM control and non-GBM treatment. The GBM and non-GBM treatment groups were given Mr. Trivedi’s biofield treatment for the assessment of its potential. Two time-lapse (10 hours prior; 10 hours after) video microscopy experiment was performed on tumor and non-tumor brain cells in six replicate (n=6). For each microscopic field, the total cell number was counted and each cell was tracked over the 20 hours period. The potential impact of biofield treatment was assessed by comparing cell death rate in both GBM and non-GBM cells before and after biofield treatment. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. The treated non-GBM cultured cells showed a significant reduction (64%) of cell death rate i.e. protective effects as compared to non-GBM control. Conclusion: Altogether, data suggests that biofield treatment has significantly increased the cell death rate of treated GBM cells and simultaneously boost the viability of normal brain cells. Therefore, biofield treatment could be a suitable alternate treatment strategy for cancer patients in near future.
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ISSN: 2329-6771
Integrative Oncology
Research Article
Trivedi et al., J Integr Oncol 2015, 4:3
http://dx.doi.org/10.4172/2329-6771.1000141
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal Volume 4 • Issue 3 • 1000141
Open Access
Keywords: Glial cell; Glioblastoma; Brain tumor; Bioeld treatment;
Time-lapse video microscopy
Introduction
Brain tumors are typically very heterogeneous, aggressive
neoplasms at the cellular level which aects both children and adults
[1]. Statistics based on cancer research institute explore that 1 in 161
individuals born today with brain cancer at dierent point of life. In the
U.S., 22,850 men and women are diagnosed with brain cancer every year,
and 15,320 deaths are caused by this disease [2]. As per World health
organization classication, glioblastoma (GBM) is also known as grade
IV astrocytoma [3]. GBM is one of the most malignant form of human
brain tumor. e mean survival time for GBM patients is approximately
12 months [4]. Time-lapse video microscopy is a technique that aids to
assess cellular behavior in real time. is technique is used to collect
a two dimensional image data at dierent time intervals. en, these
data are converted to make a movie. is unique technique is used
in various elds of cancer and stem cell biology for assessment of
cell translation, division, and death [5,6]. Presently, several strategies
are available for the treatment of GBM such as surgical techniques,
radiotherapy, chemotherapy and electrochemotherapy [7]. Apart
from commonly used chemotherapeutic agents (such as nitrosoureas
derivatives, platinum based drugs, and taxol) and gene therapy, as
a novel therapeutic modality frequently being used in cancer [8].
However, chemotherapy suers several major drawbacks such as all
tumors are not responsive to chemotherapeutic agents to some extent,
incidence of bone marrow cells damage, amenorrhea, alopecia, sexual
dysfunction and adversely aect the quality of life of patients [9].
Malignant brain tumors like GBM are very dicult to treat
now a days. e diculties to treat against GBM are due to lack of
proper preventive strategies, and unavailability of practical method
for screening [10]. erefore, new, more eective and better tolerated
anti-tumor drugs or some alternative treatment strategy are needed.
Based on the above lacunas an alternative way which may be useful to
kill or eradicates the proliferative tumorous cells and simultaneously
defense the normal brain glial cells. Bioeld treatment is an alternative
approach which may be useful to improve these unlled spaces
associated with cancer patients. e human bioeld is the energetic
matrix that surrounds the human [11]. It directly links with the cellular
activity that allows the DNA to communicate faster than light and
maintain intelligence in the organisms [12]. According to universal
principles of Maxwell’s equations and principle of reciprocity denes
electromagnetic connections related to human bioeld [13]. us, a
human has ability to harness the energy from environment/Universe
and can transmit into any object (living or non-living) around the
Globe. e object(s) always receive the energy and responded into
*Corresponding author: Dr. Snehasis Jana, Trivedi Science Research
Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City,
Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-
6660006; E-mail: publication@trivedisrl.com
Received July 07, 2015; Accepted July 21, 2015; Published July 28, 2015
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential
Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-Lapse Video
Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited.
Abstract
Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The
aim was to evaluate the impact of bioeld treatment potential on human GBM and non-GBM brain cells using two
time-lapse video microscopy technique.
Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM
treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non-
GBM control and non-GBM treatment. The GBM and non-GBM treatment groups were given Mr. Trivedi’s bioeld
treatment for the assessment of its potential. Two time-lapse (10 hours prior; 10 hours after) video microscopy
experiment was performed on tumor and non-tumor brain cells in six replicate (n=6). For each microscopic eld, the
total cell number was counted and each cell was tracked over the 20 hours period. The potential impact of bioeld
treatment was assessed by comparing cell death rate in both GBM and non-GBM cells before and after bioeld
treatment.
Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the bioeld
treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death
rate was exponentially increased (41%) after bioeld treatment as compared to control. The treated non-GBM
cultured cells showed a signicant reduction (64%) of cell death rate i.e. protective effects as compared to non-GBM
control.
Conclusion: Altogether, data suggests that bioeld treatment has signicantly increased the cell death rate of
treated GBM cells and simultaneously boost the viability of normal brain cells. Therefore, bioeld treatment could be
a suitable alternate treatment strategy for cancer patients in near future.
The Potential Impact of Biofield Treatment on Human Brain Tumor Cells:
A Time-Lapse Video Microscopy
Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Sambhu Charan Mondal2 and Snehasis Jana2*
1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA
2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026 Madhya Pradesh, India
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-
Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Page 2 of 4
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal Volume 4 • Issue 3 • 1000141
useful way that is called bioeld energy. is process is known as bioeld
treatment. e bioeld can be monitored using electromyography,
electrocardiography and electroencephalogram [14]. Mr. Mahendra
Trivedi’s unique bioeld treatment (e Trivedi Eect) has been
well known for altered characteristics features of microbes [15-17],
improved the overall productivity of crops [18,19], and also transform
the structural, physical and chemical properties of materials [20-23].
Exposure to bioeld energy caused an increase in medicinal property,
growth, and anatomical characteristics of ashwagandha [24].
On the basis of above facts and literature, present study was
undertaken to evaluate the impact of Mr. Trivedi’s bioeld treatment
on human GBM brain tumor cells.
Materials and Methods
Human U87 glioblastoma cells were received from California
pacic medical center research institute, bioscience laboratory, San
Francisco, CA. e cultures were prepared and maintained as per the
protocols.
Study design and bioeld treatment strategy
e GBM cultured cells were divided into two groups viz. GBM
control and GBM treatment. Besides this, normal brain cultured cells
were also divided into two groups such as non-GBM control and
non-GBM treatment. Prior to experiment, each cell culture plate was
prepared with six replicates (n=6) of both GBM and non-GBM cells.
Each cell culture plate was grown side-by-side in sub-compartments of
one culture plate, so that both types of cells were exposed to treatment
and without treatment simultaneously. One set of culture plates (GBM
and non-GBM treatment) were handed over to Mr. Trivedi for bioeld
treatment under laboratory condition. Mr. Trivedi provided the
treatment through his energy transmission process to the culture plates
in front of the time-lapse microscope to deliver bioeld treatment from
outside a plexiglass environmental chamber attached to the microscope
without touching them. Another set was considered as control
experiment in which no treatment was delivered. Aer treatment, all
the culture plates were analyzed for assessment of bioeld treatment
potential. In brief, during the experiment involving bioeld treatment,
Mr. Trivedi was escorted to the laboratory and seated a short distance
(approximately 20 cm) from the time-lapse microscope (Figure 1).
During the control experiment, the cells were handled identically but Mr.
Trivedi did not visit the laboratory. A laboratory technician placed the
cell culture plates inside of an environmental chamber for examination
by time-lapse microscopy without knowledge of the eventual treatment
condition for the samples. e following day, laboratory technician has
converted the acquired images into Quicktime movies and transferred
the movies to a database using blinding codes for analysis.
Two time-lapse video microscopy technique
Two time-lapse video microscopy experiments were followed in
human brain tumor GBM cells and normal brain cells for 20 hours time
cycle. Microscopic images were acquired in every 5 minutes interval,
from 10 hours prior and 10 hours following the treatment time i.e. 20
hours for each experiment, and compiled into time lapse videos. Figure
2 depicts the time schedule for two time-lapse video microscopy of cell
cultures [25].
Criteria for analysis of cell count
For each microscopic eld, the total number of cells were counted
and each cell was tracked over the 20 hours period. Cells that entered
the microscopic eld aer the initial frame were neither included,
nor identied as dead at the start of the video. Cell death, divisions
and emigrations (cells migrating out of the microscopic eld) were
counted for varying numbers of cells and count was made every single
hour. Aer that, all the data were collected and entered into excel
spreadsheets, the blinding codes were revealed and the results were
sorted according to experimental group for analysis.
Results and Discussion
Since, brain tumors especially GBM are the most critical medical
problem aecting major part of population, physician suggests
chemoprevention along with radiation strategies to reduce the
incidence and mortality rate. However, there are scientic evidence and
large number of in vitro studies already reported for bioeld treatment
and its eect in cancer cells [26,27]. Previous studies indicated that
bioeld energy showed an inhibitory eect on metastasis and immune
responses in cancer cell models [11,26,28].
An exponential growth model was applied to the study data derived
through time-lapse video microscopic technique which aims to take
into account potential inuence on cell death rates due to change the
number of cells in each microscopic eld throughout the observation
period. Death counts during 10 hours period of observation were
converted to rates per cell by the following formula:
Death rate = ln(N(t-1)) – ln(N(t-1) – d(t))
Where, N(t-1) is the number of cells at the start of the time period
and d(t) is the number of deaths during the time period. ese estimates
Figure 1: Schematic diagram represents the physical arrangement of bioeld
treatment session from a short distance (approximately 20 cm), outside a
plexiglass environmental chamber attached to the microscope.
Figure 2: Schematic diagram represents the schedule for time-lapse video
microscopy of cell cultures, A) treatment and B) control.
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-
Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Page 3 of 4
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal Volume 4 • Issue 3 • 1000141
very popular and scientically studied [31,32]. erefore, Mr. Trivedi’s
bioeld treatment could be an eective (with no existing side eects),
cost eective (no drugs were used) and alternate approach for cancer
chemotherapy. Research on bioeld treatment and its impact on cancer
growth has been continuously studied by researchers, which might
works as alternate and complementary medicine to prolong the life of
cancerous patients along with quality life.
Overall, the study data indicates that bioeld treatment has
signicantly increased (41%) the death rate of GBM brain tumor
cells as compared to untreated GBM cells. Besides this, non-GBM
treatment cultured cells showed a signicant reduction of cell death
rate (64%) as compared to normal brain cells (Figure 5). Based on the
obtained results, it is expected that bioeld treatment could be novel,
cost eective and an alternative advanced strategy towards brain tumor
patients.
Conclusions
In conclusion, the study results suggest that the bioeld treatment
has signicantly increased (41%) the cell death rate of GBM treatment
brain tumor cells, as compared to GBM control cells. In addition,
bioeld treatment also showed a signicant reduction of cell death
rate (64%) in non-GBM treated culture cells i.e. protective eect as
compared to non-GBM brain cells. Based on the results, the inexpensive
are based on an assumption that counts are dependent on the number
of cells present [6].
e potential impact of bioeld treatment on GBM brain culture
cells was assessed by comparing cell death rates before and aer
treatment. In non-GBM (control) group, values for cell division
were not signicantly analyzed due to very low cell divisions rate, as
the majority of the cell counts being zero throughout the 20 hours
observation period. is lack of cell division under control conditions
for the normal brain cells indicated that the culture conditions were
not sucient to keep the normal cells viable throughout the extended
time in culture. is is oen may be the case with cultures of normal
cells, whereas cancerous cells typically survive in culture more readily.
Comparing the total number of cell deaths over the 10 hours prior
and 10 hours following the treatment time (T = 0) for the four groups of
cells and dividing these values by the initial cell counts for each group
gives a rough estimation of the potential eect of treatment. Analysis
using the exponential growth model indicates that the cell death rate
in GBM treatment cultured cells increased aer bioeld treatment
however, no change in cell death rate was observed in GBM control
cultured cells. Moreover, the non-GBM treatment cultured cells has
appeared to be protected from a baseline increase in cell death rate
observed in the untreated non-GBM control cultured cells (Figure 3).
e increase in cell death rate as observed in the untreated non-GBM
control cultured cells was consistent while the cancer cell viability was
more robust [29].
Pre-treatment values were subtracted from post-treatment values
to evaluate the relative change in cell death rate aer treatment time (T
= 0) and referred as delta (δ). e δ values (as measured from before
to aer treatment per initial cell) due to bioeld treatment relative
to controls for GBM cells and non-GBM cells are shown in Figure
4. e advantage of bioeld treatment is that they are inexpensive as
compared to other types of cancer therapies. According to a recent
report regarding bioeld treatment later on, it was approved by the
German equivalent of the FDA. It was emphasized that cancer patients
have experienced healing with bioeld treatment. Nowadays, many
scientists and cutting edge practitioners believed that the secrets
of regeneration and healing lie not only on costly medical drugs or
expensive medical treatments, but also in the body’s own Quantum
Energy Bioeld (QEB) [30].
Current cancer therapies have certain limitations which include
serious side eects like enormous toxicity, altered immune system and
high treatment cost. Many ancient bioeld therapies for cancer are
practiced around the world, among then Qigong therapy for cancer is
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Control Treatment Control Treatment
GBM Non GBM
Cell death rate
(Per hour)
Before After
Figure 3: Comparison of cell death rate before and after bioeld treatment.
GBM: Glioblastoma
-0.5
-0.4
-0.3
-0.2
-0.1
0
0.1
0.2
Change in cell death rate (δ)
GBM
Non-GBM
Figure 4: The change in cell death rate (δ) of both glioblastoma brain tumor
cells and normal brain glial cells after treatment time (T = 0) due to bioeld
treatment relative to controls.
Figure 5: Percent change of cell death after 20 hours treatment period with
respect to control.
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The Potential Impact of Bioeld Treatment on Human Brain Tumor Cells: A Time-
Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-6771.1000141
Page 4 of 4
J Integr Oncol
ISSN: 2329-6771 JIO, an open access journal Volume 4 • Issue 3 • 1000141
bioeld treatment approach could be used in glioblastoma brain tumor
patients in near future to improve the quality of life.
Acknowledgement
Trivedi science, Trivedi testimonials and Trivedi master wellness gratefully
acknowledged to the whole team of the bioscience laboratories, 665, Third Street,
San Francisco, CA for their support.
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Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The
Potential Impact of Bioeld Treatment on Human Brain Tumor Cells: A
Time-Lapse Video Microscopy. J Integr Oncol 4: 141. doi:10.4172/2329-
6771.1000141
... Trivedi et al. [121] Biofield treatment exponentially increased (41%) the cell death rate of human glioblastoma, compared to control, which remained relatively constant throughout the 20-h testing period. The treated healthy brain cultured cells showed a significant reduction (64%) of the death rate. ...
... Trivedi et al. [121] tested the effects of a noncontact biofield treatment over in vitro cultures of human glioblastoma cells and healthy brain cells compared to untreated controls. The practitioner, seated at a short distance from the cells, was instructed to deliver the treatment outside an acrylic environmental chamber that enclosed the cells and microscope. ...
... The authors found an exponential increase in the cell death rate (41%) after treatment compared to control, which remained relatively constant throughout the 20-h testing period. The treated healthy brain cultured cells showed a significant reduction (64%) of cell death rate, suggesting a protective effect of the biofield treatment [121]. Similar results were found by Yan et al. [118] while studying Yan Xin "Qigong's" effect over small-cell lung cancer cells. ...
Article
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Practices such as “Reiki”, therapeutic touch, healing touch, and external “Qigong” have been regarded as some form of “energy medicine” or “biofield therapy”. The biofield concept has been studied and debated by researchers of distinct areas of expertise, and although the phenomenon was sometimes described as physically related to electromagnetics, other factors such as “subtle energy” and focused intention might be involved. These nonconventional practices integrate contact and non-contact techniques, and those dealing with so-called distant healing interventions are perhaps the most difficult to understand and accept. Practitioners describe these so-called nonlocal interventions as involving intentional factors and particular states of consciousness. With a spiritual mindset and a particular state of awareness, compassion is said to work out as a catalyst to produce physiological and physical changes through mechanisms that are still unknown. At the body level, these vegetative changes might be related to individual self-perception variations as part of the body neurovegetative feedback system of regulation. Further mechanisms are difficult to document and measure, and might be more accessible to research by using physical signal detectors, chemical dynamics methods, detectors using biological materials, detectors using living sensors, and detectors using the human body. The growing interest in these practices and the considerable amount of research exploring their effects and clinical applications encouraged this narrative review, which aims to provide an easy to consult partial overview of the history, theory and findings of quantitative research strategies exploring non-contact biofield-based practices. This work also aims to stimulate the reader’s mind with the raised hypotheses, catalyzing further research on the subject to confirm or deny the reported outcomes.
... Improvement of the physicochemical properties of a substance is a challenging task. In this scenario, The Trivedi Effect ® -Consciousness Energy Healing Treatment have the extraordinary abilities to transform the properties of many living and non-living object(s) [11][12][13][14][15]. The Trivedi Effect ® is a natural and only scientifically proven phenomenon in which a person can harness this inherently intelligent energy and transmit it anywhere on the planet through the possible mediation of neutrinos [16]. ...
... The Trivedi Effect ® -Consciousness Energy Healing Treatment (Biofield Energy Healing Treatment) could be an economical approach to obtain a better desirable L-cysteine with improved physicochemical properties for the food, cosmetic, pharmaceutical/ nutraceutical, and other industries. Therefore, this study was designed and evaluated the LC-MS based structural characterization and the isotopic abundance ratios of P M+1 /P M ( 2 H/ 1 H or 13 ...
... These CAM therapies have been adopted by most of the U.S.A. population [20]. The Trivedi Effect ® -Consciousness Energy Healing Treatment also has the significant impact on the transformation of metals, ceramics, and polymer [21][22][23], organic compounds [24], microorganisms [25,26] the productivity of crops [27,28] and cancer cells [29,30]. ...
... Such therapies have been well accepted by most of the U.S.A. population with advantages [20]. The Trivedi Effect ® -Consciousness Energy Treatment also reported with significant results altering the intrinsic properties of metals, organic compounds, ceramics, polymers, microbes, and cancer cell line; improved yield, productivity, and quality of crops, improved bioavailability of pharmaceutical and nutraceutical compounds, and altered the isotopic abundance ratio ISSN: 2469-4185 [21][22][23][24][25][26][27][28][29][30][31]. ...
... Energy Healing Treatment has also reported with altering the physical and thermal properties of organic compounds, polymers, ceramics, and metals [29][30][31][32][33][34], improved productivity of crops [35], and healing cancer [36]. isotopic abundance ratio of L-ascorbic acid compared to the control sample. ...
Article
Ascorbic acid is a water-soluble vitamin (Vitamin C) essential for both the plants and animals for the metabolic process. In this study, the liquid chromatography-mass spectrometry (LC-MS) analytical technique was used to characterize the structural properties and isotopic abundance ratio to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on L-ascorbic acid compared to the control sample. The ascorbic acid sample was divided into control and treated parts. Only the treated part received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mahendra Kumar Trivedi. The control and treated samples showed a chromatographic peak at retention time (Rt) 1.8 minutes exhibited the deprotonated molecular ion peak at m/z 175 (M-H)- (calculated for C6H7O6-, 175.02) in the mass spectra. The peak area of the treated sample (12817614.01) was significantly increased by 8.81% compared to the control sample (11779918.9). The LC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the treated ascorbic acid was significantly increased by 23.22% compared with the control sample. Thus,13C, 2H, and17O contributions from (C6H7O6)- to m/z 176 in the treated ascorbic acid were significantly increased compared with the control sample. The increased isotopic composition of the treated ascorbic acid might have altered the neutron to proton ratio in the nucleus. The changes in isotopic abundance could be due to changes in nuclei possibly through the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio and peak area of the treated ascorbic acid may increase the intra-atomic bond strength and its stability. This novel ascorbic acid after the Trivedi Effect®-Consciousness Energy Healing Treatment would be very useful to design more efficacious pharmaceutical formulations against scurvy, obesity, cardiovascular diseases, hypertension, rheumatoid arthritis, Alzheimer's disease, cancer, etc.
... and medicines in biological systems [28,29]. The Trivedi Effect ® -Consciousness Energy Healing have been accepted worldwide, which has been scientifically studies on various models in the materials science [30,31], agriculture science [32], microbiology [33,34], biotechnology [35], and improved bioavailability of various compounds [36,37], skin health [38,39], nutraceuticals [40], cancer research [41], bone health [42,43], overall human health and wellness. The present study was planned to evaluate different brain and lung biomarkers in male Sprague Dawley rats fed with VDD diet and Biofield Treated test formulation/per se to the animals by a renowned Biofield Energy Healer. ...
Article
A novel proprietary test formulation was designed which included minerals, vitamins, β-carotene, cannabidiol isolate,and Panax ginseng extract. This present study was evaluated the impact of the Trivedi Effect® on novel proprietary test formulation in male Sprague Dawley rats, fed with vitamin D3 deficiency diet (VDD). The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of 25-OH Vit. D3 was measured in brain homogenate, which was found to be increased by 20.13%, 24.12%, 45.86%, 14.79%, and 29.96% in the G5 group treated with Biofield Treated test formulation, Biofield Energy Treatment per se to the animals (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15 (G8), and untreated test formulation to the Biofield Energy Treated animals (G9) groups respectively, as compared with the disease control (G2) group. Brain acetylcholine (ACh) level was increased by 61.33% in the G7 group as compared with the untreated test formulation (G4) group. The expression of interleukin-6 (IL-6) was significantly reduced by 43.44% (p≤0.01), 30.93%, 21.42%, 45.99% (p≤0.01), and 60.85% (p≤0.01), respectively as compared with the G4. Lung pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) level was significantly reduced in the G5, G6, G7, and G8 by 24.86%, 32.55% (p≤0.01), 30.12% (p≤0.01), and 42.69% (p≤0.01), respectively, as compared with the G4 group. Altogether, the Biofield Treated test formulation and/or per se treatment to the animals significantly improved the levels of active form of vitamin D3 metabolite (25-OH Vit D3) and neurotransmitter (ACh); consequently significantly lowered the expression of proinflammatory cytokines (IL-6 and TNF-α). Therefore, the energized test formulation or per se treatment could be effectively useful against neuronal damage and inflammation for the management of brain disorders such as Alzheimer’s disease, dementias, brain cancer, epilepsy and other seizure disorders, mental disorders, and Parkinson’s. Thus, the results showed a significant slowdown of disease progression and all other disease-related complications/symptoms in the preventive Biofield Energy Treatment group per se and the Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group.
... These therapies have been accepted by most of the U.S.A. population with several advantages [16]. The impact of The Trivedi Effect ® -Biofield Energy Healing Treatment had been proved scientifically with significant outcome in different fields, i.e., pharmaceuticals and nutraceuticals [17,18], materials science [19,20], agricultural science [21,22], microbiology [23,24], cancer research [25,26], etc. The Trivedi Effect ® -Biofield Energy Healing Treatment could be an economical approach and solution for the practical challenges faced by ofloxacin with respect to the physicochemical properties for designing better pharmaceuticals and nutraceutical formulations. ...
... The National Institutes of Health (NIH) and the National Center for Complementary and Alternative Medicine (NCCAM) applaud and included the Energy therapy under Complementary and Alternative Medicine (CAM) category along with other complementary therapies (i.e., Yoga, Ayurveda, Reiki, etc.) which has been accepted by the most of the U.S.A. people [18,19]. The outstanding outcome of the Trivedi Effect ® -Consciousness Energy Healing Treatment also on polymers [20,21], nutraceuticals [22,23], pharmaceuticals [24][25][26], organic compounds [27][28][29], cancer cells [30,31], microorganisms [32,33], and crops [34,35] have been widely reported with scientifically experimental data. Therefore, one such experiment was designed to determine the impact of the Trivedi Effect ® -Consciousness Energy Healing Treatment on the physicochemical properties of tellurium metal powder using modern analytical techniques. ...
Article
Ofloxacin is an antibiotic, useful against the number of bacterial infections. This scientific investigation was performed to identify the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of ofloxacin using sophisticated analytical techniques. Ofloxacin sample was divided into control and treated parts. Only the treated ofloxacin received the Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The LC-MS spectra of both the samples of ofloxacin at retention time 3 minutes exhibited the mass of the protonated molecular ion peak at m/z 362.17 (M+H)+. The chromatographic peak area% of the treated ofloxacin (52.4%) was increased by 2.03% compared to the control sample (51.36%). The LC-MS based isotopic abundance ratio of PM+1/PM in the Biofield Treated ofloxacin was significantly increased by 22.43% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM in the Biofield Treated ofloxacin was significantly increased by 19.24% compared with the control sample. The LC-MS and GC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 15N/14N or 13C/12C or 17O/16O) was significantly increased in the Biofield Treated ofloxacin as compared to the control sample. Thus,2H, 15N, 13C, and17O contributions from (C18H21FN3O4)+ to m/z 363.17 in the treated ofloxacin were significantly increased compared with the control sample. The increased isotopic abundance ratio of the Trivedi Effect®-Consciousness Energy Healing Treated ofloxacin may increase the intra-atomic bond strength and increase its physical stability. The new form of treated ofloxacin would be more stable, better soluble, and bioavailable compared to the control sample. It would be more useful to design efficacious pharmaceutical formulations that might offer better therapeutic response against infections in the urethra, urinary tract, gonorrhoea, pneumonia, infectious diarrhoea, bronchitis, cellulitis, bacterial infection of the eye and ear, multidrug-resistant tuberculosis, prostatitis, otitis media, plague, etc.
Article
The aim of the study was to evaluate the immunomodulatory activity of the Biofield Treated/Blessed proprietary test formulation consisting of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3) in male Sprague Dawley rats. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment/Blessing, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi remotely. Additionally, three group of animals were also received Biofield Energy Treatment per se (at day -15) under similar conditions. The parameters were assessed such as immune biomarkers (IgM, IgG, IgA, IgE, CD4+, CD8+, and CD28+), biochemistry and hematology and histopathology. The experimental results showed IgG level was significantly increased by 10.70% and 8.03% in the G6 (Biofield Energy Treatment per se at day -15) and G8 (Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15) groups, respectively as compared with untreated test formulation (G4). Additionally, CD8+ count was significantly increased by 20.67% in the G8 group, while CD28+ count was significantly increased by 11.70%, 8.32%, and 9.82% in the G7 (Biofield Energy Treated test formulation at day -15), G8, and G9 (Biofield Treatment per se (day -15) to animals plus untreated test formulation) groups, respectively after Biofield Energy Treatment to the animals as compared with the untreated test formulation. In hematological analysis, platelet count was increased in the G5, G6, G7, G8, and G9 groups by 40.69%, 27.95%, 26.67%, 38.58%, and 28.28%, respectively compared with the disease control (G2) group. Biochemical parameters showed significant decrease in the level of creatinine by 32.14% in the G9 group as compared with the G2 group. Further, animal body weight, feed intake, relative organ weight, and histopathological findings of all the tested groups did not show any abnormal findings with respect to the safe and non-toxic treatment strategies. Overall, the experimental data concluded that the Biofield Energy Treated/Blessed test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation has the significant capacity for immunomodulatory effect, stress management and anti-aging by improving overall health.
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Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The combination of biofield treatment along with administration of chemical additives demonstrated the best results with statistically increased yields and higher pest resistance in both test cropping systems. The specific mechanisms that lead to these preliminary results have yet to be determined.
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Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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This study tested the Null Hypothesis for the effect of BioField Energy applied to two separate crops under typical growing conditions, namely ginseng and organic blueberry in commercial plantings in Wisconsin and California, respectively. Following treatment to replicated plots in standard experimental design, data were collected at harvest for yield quantity and quality. Ginseng plants treated both pre-harvest and a combination of pre- and post-harvest showed market grade increases of 33.3% and 40.0%, respectively. Point of sale gross return for this crop is dependent upon tuber quality, and from these data the economics of these treatments were calculated. Based on stand adjusted yields and quality values, a combination of pre- and post-harvest treatment increased gross income by 57.4%. The second crop showed similar trends in positive responses. In the two blueberry varieties studied, Emerald treated plants showed 96% statistical increase in yield, while Jewel showed 31% increase. At the time of treatment, each variety was in a different stage of flowering. The Emerald variety was in the flowering stage, and Jewel was predominately in the fruiting stage. Both treated cultivars however demons-trated increased yield quantity and quality. The specific mechanisms that lead to these pre-liminary results need further investigation.
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Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC –51299], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists Results: The results indicated that Mr.Trivedi’s energy has changed 9 of 27 biochemical characteristics of Enterococcus faecalis along with significant changes in susceptibility pattern in 5 of 31 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy/treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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Purpose While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists. Results The results indicated that Mr.Trivedi’s energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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Zirconium oxide and silicon dioxide powders are selected and subjected to a non-contact Biofield energy known to be transmitted by Mahendra Kumar Trivedi. Particle sizes d50 and d99 showed up to 71.5 percent decrease indicating that the energy had caused deformation and fracture as if the powders have been subjected to high energy milling. This is also supported by increase in specific surface area up to 19.48 percent. In the present investigation Zirconium oxide and silicon dioxide powders are exposed to Bio-field. Both the exposed and unexposed powders are later characterized by various techniques. The treated powders when characterized by X-ray diffraction are found to exhibit significant increase and decrease in the lattice parameters of the unit cell, crystallite size and density. The lattice parameters are then used to compute the molecular weight and total number of protons and neutrons in the molecule, which showed an increase up to 0.24 and decrease up to 0.31 percent. It is speculated that the Biofield energy transmitted by Mr. Trivedi is acting on the nucleus in the atoms through some reversible weak interaction of larger cross section causing changes in the proton to neutron ratios and thus energy to mass and mass to energy. Thus the effect is felt by all the atoms, and hence the unit cell, single crystal grain and grain boundaries. The stresses generated in turn may have caused deformation and fracture of the weak interfaces in the polycrystalline powders such as the crystallite and grain boundaries.
Article
Zirconium oxide and silicon dioxide powders are selected and subjected to a non-contact Biofield energy known to be transmitted by Mahendra Kumar Trivedi. Particle sizes d50 and d99 showed up to 71.5 percent decrease indicating that the energy had caused deformation and fracture as if the powders have been subjected to high energy milling. This is also supported by increase in specific surface area up to 19.48 percent. In the present investigation Zirconium oxide and silicon dioxide powders are exposed to Bio-field. Both the exposed and unexposed powders are later characterized by various techniques. The treated powders when characterized by X-ray diffraction are found to exhibit significant increase and decrease in the lattice parameters of the unit cell, crystallite size and density. The lattice parameters are then used to compute the molecular weight and total number of protons and neutrons in the molecule, which showed an increase up to 0.24 and decrease up to 0.31 percent. It is speculated that the Biofield energy transmitted by Mr. Trivedi is acting on the nucleus in the atoms through some reversible weak interaction of larger cross section causing changes in the proton to neutron ratios and thus energy to mass and mass to energy. Thus the effect is felt by all the atoms, and hence the unit cell, single crystal grain and grain boundaries. The stresses generated in turn may have caused deformation and fracture of the weak interfaces in the polycrystalline powders such as the crystallite and grain boundaries.
Article
ilicon, tin and lead powders belong to group IV in periodic table and exhibit decreasing semi conducting nature towards the bottom of the group. These are very useful in producing non ferrous powder metallurgy components. In the present investigation silicon, tin and lead powders are exposed to bio field. Both the exposed and unexposed powders are later characterized by various techniques. The average particle size, after an initial decrease is found to increase with increase in number of days after treatment although the size is lee than that exhibited by untreated powder, suggesting the operation of competing mechanisms fracture and sintering. The BET surface area increased slightly in silicon powder but did not change in tin and lead powders. SEM photographs showed that samples exposed to bio field after 20 days showed fracture paths and fractures at inter and intra particle boundaries in treated powders. Thermal analysis indicated a decrease in heat of reaction and decrease in mass in treated samples. X-ray diffraction of the powder samples indicated both increase and decrease in crystallite size, unit cell volume and molecular weight of samples exposed to bio field even after 179 days. These results indicate that the properties of the metallic powders can be controlled even up to atomic level by exposing to bio field.
Article
The fourth edition of the World Health Organization (WHO) classification of tumours of the central nervous system, published in 2007, lists several new entities, including angiocentric glioma, papillary glioneuronal tumour, rosette-forming glioneuronal tumour of the fourth ventricle, papillary tumour of the pineal region, pituicytoma and spindle cell oncocytoma of the adenohypophysis. Histological variants were added if there was evidence of a different age distribution, location, genetic profile or clinical behaviour; these included pilomyxoid astrocytoma, anaplastic medulloblastoma and medulloblastoma with extensive nodularity. The WHO grading scheme and the sections on genetic profiles were updated and the rhabdoid tumour predisposition syndrome was added to the list of familial tumour syndromes typically involving the nervous system. As in the previous, 2000 edition of the WHO ‘Blue Book', the classification is accompanied by a concise commentary on clinico-pathological characteristics of each tumour type. The 2007 WHO classification is based on the consensus of an international Working Group of 25 pathologists and geneticists, as well as contributions from more than 70 international experts overall, and is presented as the standard for the definition of brain tumours to the clinical oncology and cancer research communities world-wide