Lipase acts as catalyst in transesterification reactions, where transposition of alkyl radicals occurs between a triglyceride and an ester, an acid, or even an alcohol in the presence of a catalyst. An example for the last case is biodiesel production, which yields glycerol. The enzyme Lipolase (Thermomyces lanuginosus lipase) produced by submerged fermentation of genetically modified Aspergillus
... [Show full abstract] oryzae was studied. The transesterification activity test indicated that the highest activity was achieved at 40°C with moisture content of 30% in the biocatalyst. Both temperature and moisture content significantly influenced the process. The design variables analyzed independently, as well as their interaction, were significant in a confidence interval of 95%, indicating that the proposed model is valid. This is an abstract of a paper presented at the CHISA 2012 - 20th International Congress of Chemical and Process Engineering and PRES 2012 - 15th Conference PRES (Prague, Czech Republic 8/25-29/2012).