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An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential Using BACTEC 460/MGIT-TB System

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The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3.33%, 3.33% and 400.6%, respectively, as compared to control in extensive drug resistance (XDR) strains. Pyrazinamide showed 300% susceptibility as compared to control in multidrug resistance (MDR) strains. Isoniazide did not show any improvement of susceptibility pattern against treated either in XDR or MDR strains of Mycobacterium as compared to control. Besides susceptibility, the resistance pattern of treated group was reduced in case of isoniazide (26.7%), rifampicin (27.6%), pyrazinamide (31.4%), ethambutol (33.43%) and streptomycin (41.3%) as compared to the untreated group of XDR strains. The MIC values of few antimicrobials were also altered in the treated group of Mycobacterium smegmatis. There was a significant reduction observed in MIC values of linezolid (8.0 to 2.0 µg/ml) and tobramycin (2.0 to 1.0 µg/ml); however, very slight changes occurred in the remaining antimicrobials of treated samples. There was no change of MIC values in the strain of Mycobacterium tuberculosis after biofield treatment. Biofield treatment effect on Mycobacterium against anti-tubercular drugs might be due to altered ligand-receptor/protein interactions at either enzymatic and/or genetic level with respect to anti-mycobacterium susceptibility and MIC values of antimicrobials.
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An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential
Using BACTEC 460/MGIT-TB System
Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Sambhu Charan Mondal2 and Snehasis Jana2*
1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA
2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026 Madhya Pradesh, India
*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,
Bhopal-462026 Madhya Pradesh, India, Tel: + 91-755-6660006; E-mail: publication@trivedisrl.com
Received date: June 22, 2015; Accepted date: July 20, 2015; Published date: July 27, 2015
Copyright: ©2015 Trivedi MK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to
antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC
460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture
Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials
(Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and
streptomycin in treated samples showed increased susceptibility as 3.33%, 3.33% and 400.6%, respectively, as
compared to control in extensive drug resistance (XDR) strains. Pyrazinamide showed 300% susceptibility as
compared to control in multidrug resistance (MDR) strains. Isoniazide did not show any improvement of susceptibility
pattern against treated either in XDR or MDR strains of Mycobacterium as compared to control. Besides
susceptibility, the resistance pattern of treated group was reduced in case of isoniazide (26.7%), rifampicin (27.6%),
pyrazinamide (31.4%), ethambutol (33.43%) and streptomycin (41.3%) as compared to the untreated group of XDR
strains. The MIC values of few antimicrobials were also altered in the treated group of Mycobacterium smegmatis.
There was a significant reduction observed in MIC values of linezolid (8.0 to 2.0 µg/ml) and tobramycin (2.0 to 1.0
µg/ml); however, very slight changes occurred in the remaining antimicrobials of treated samples. There was no
change of MIC values in the strain of Mycobacterium tuberculosis after biofield treatment. Biofield treatment effect
on Mycobacterium against anti-tubercular drugs might be due to altered ligand-receptor/protein interactions at either
enzymatic and/or genetic level with respect to anti-mycobacterium susceptibility and MIC values of antimicrobials.
Keywords: Antimycobacterial susceptibility; Biofield treatment;
MDR-TB; XDR-TB; BACTEC 460/MGIT-TB; MIC
Introduction
Global tuberculosis control is facing major challenges now days.
Multidrug resistance of
Mycobacterium tuberculosis
(MDR-TB) has
recently become a serious public health burden. Extensively drug
resistant TB (XDR-TB; MDR resistance plus resistance to a
fluoroquinolones and an aminoglycoside and to at least one of the
three injectable drugs kanamycin, capreomycin and amikacin) is a
form of tuberculosis which is now a serious threat to human life.
Antimycobacterial susceptibility testing is necessary for the proper
treatment of patients with tuberculosis through a multiple drug
regimen. The key reasons for the development of MDR-TB are due to
ongoing bacterial mutation and early discontinuation of treatment [1].
Drug resistance in
M. tuberculosis
are attributed to random mutations
in the mycobacterial genome. All wild type population of
M.
tuberculosis
contain a few mutant strains that are resistant to one of
the drugs. These drug resistant strains emerge and become a dominant
clone of the population when chemotherapy is intermittent or
otherwise inadequate. Tuberculosis has become a threat in the modern
era of antimicrobial warfare, because its unique characteristics give it
enormous potential for developing resistance to even the strongest
antimicrobials [2].
There are various major lacunas associated with currently used
directly observed treatment short course (DOTS) regimen in both
MDR as well as XDR-TB. The duration and complexity of treatment
result in non-adherence, which leads to sub-optimal response like
failure, relapse and ultimately developed resistance. Manifestation of
adverse effects of anti-TB drugs also contributes a problem of non-
adherence. Co-infection of TB and HIV is a problem by itself and
prophylactic therapy of latent TB (TB infection without symptoms)
with isoniazid is also associated with problems of non-adherence [3-5].
Based on above lacunas there is an urgent need for an alternative
way to improve tuberculosis therapy by either enhancing the
application of existing agents by means of some alternative strategy or
introducing new drugs. Biofield treatment is an alternative approach
which may be useful to improve these lacunas associated with
mycobacterial resistance. The human biofield’s is the energetic matrix
that surrounds the human [6]. It directly links with the cellular activity
that allows the DNA to communicate faster than light and maintain
intelligence in the organisms [7]. According to universal principles of
Maxwell's equations and the principle of reciprocity, it defines
electromagnetic connections related to the human biofield [8].
Afterward, Harold Saton Burr had performed the detailed studies on
the correlation of electric current with physiological process and
concluded that every single process in the human body had an
electrical significance [9]. According to Rivera-Ruiz et al. 2008,
reported that electrocardiography has been extensively used to
measure the biofield of human body [10]. Thus, the cumulative effect
of bio-magnetic field and electric field surrounds the human body is
defined as biofield. The energy associated with this field is considered
Mycobacterial Diseases Trivedi et al., Mycobact Dis 2015, 5:4
http://dx.doi.org/10.4172/2161-1068.1000189
Research Article Open Access
Mycobact Dis
ISSN:2161-1068 MDTL, an open access journal Volume 5 • Issue 4 • 1000189
as biofield energy and it can also be monitored by using
electromyography (EMG) and electroencephalogram (EEG) [11].
Mr. Mahendra Trivedi’s biofield treatment (The Trivedi effect®) has
been known to transform the unique structural, physical and chemical
properties of materials [12,13], improved the productivity of crops
[14,15] and altered characteristic features of microbes [16-19].
Recently, the BACTEC Mycobacteria Growth Indicator Tube
System (BACTEC 960/MGIT), a newly developed non-radiometric,
fully automated, and continuous monitoring system, has been
introduced as an alternative to the radiometric BACTEC 460 for
growth and detection of mycobacteria. BACTEC 960/MGIT is a
suitable tool for the detection of
M. tuberculosis
and other
mycobacterial species, comprising wide variations in diagnostic
performance [20]. The aim of this study was to determine the impact
of biofield treatment on XRD and MDR strains of
Mycobacterium
against susceptibility patterns of antitubercular drugs.
Figure 1: Percentage change of resistance and susceptibility pattern
of antitubercular drugs after biofield treatment with respect to
control in extensive drug resistance (XDR) strains of
Mycobacterium
.
Materials and Methods
Mycobacterial two ATCC strains (
Mycobacterium smegmatis
14468
and
Mycobacterium tuberculosis
25177) were procured from
MicroBioLogics, Inc., USA and other 39 MDR and XDR strains were
procured from stored stock cultures, Department of laboratory
medicine, Microbiology, P.D.Hinduja National Hospital and Medical
Research Centre, Mumbai. The following antimycobacterial agents
and twelve antimicrobials were purchased from Sigma-Aldrich such as
isoniazide (H), rifampicin (R), pyrazinamide (Z), ethambutol (E),
streptomycin (S), kanamycin, ethionamide, p-amino salicylate-Na
(PAS), ofloxacin, amikacin, moxifloxacin, clofazime and capreomycin.
Assessment of anti-mycobacterial susceptibility and
Minimum Inhibitory Concentration (MIC) of antimicrobials
Antimycobacterials susceptibility was assessed using all three
generations antitubercular drugs (Table 1) and MIC values were
calculated 12 selected antimicrobials such as linezolid, clarithromycin,
amikacin, cefoxitin, cefriaxone, imipenem, minocycline, tobramycin,
ciprofloxacin, gatifloxacin, amoxicillin/clavulanic acid and
trimethoprim/sulfamethoxazole. Antimicrobics were used at the
microgram level to assess the MIC breakpoint values as per Clinical
and Laboratory Standards Institute (CLSI) guidelines.
Biofield Treatment Strategy and Experimental Design
Mycobacterial strains were grouped into two categories: Group I
consisted of XDR and MDR stored stock strains and Group II was
consisted of two ATCC strains. The group I:30 XDR and 9 MDR lab
isolates from stock cultures were revived in 2 sets of MGIT vials. The
first set was considered as control. No treatment was given to this set.
The second set of MGIT vials were considered as treated group. After
biofield treatment, both control and treated samples were analysed
after 7 days for anti-mycobacterial susceptibility as per the standard
protocols. Group II:Two ATCC strains such as
M. smegmatis
and
M.
tuberculosis
were revived on two separate MGIT vials. The first set of
MGIT vials were considered as control and no treatment was given to
this set. The second set of MGIT vials were considered as treated
group. The second set of MGIT vials of both Groups I and Group II
samples in sealed pack were handed over to Mr. Trivedi for biofield
treatment under laboratory condition. Mr. Trivedi provided the
treatment through his energy transmission process to the treatment
groups without touching the samples. After treatment, all samples
were handed over in the same condition and stored for analysis. Both
control and treated samples were analyzed after 7 days for
antimycobacterial susceptibility as per the standard protocols. The
MIC was considered as the end point determination.
The MGIT vials were inoculated with the mycobacterial cultures
and loaded on MGIT 460 machine till the machine flagged positive
according to standard protocol. The antimycobacterial sensitivity to all
tubercular drugs was carried out using 12 B BACTEC vials on the
BACTEC 460 TB machine in sputum samples as per the standard
procedure except for pyrazinamide that was performed on the MGIT
960. The percentage responses were calculated with the help of
following formula:
%Response=[(Treated-Control)/Control] × 100
Results and Discussion
Antimycobacterial susceptibility and MIC of antimicrobials
The aim of this study was to determine the impact of biofield
treatment on susceptibility patterns of anti-tubercular drugs to XRD
and MDR strains of
Mycobacterium
. For this purpose we had selected
all three generations drugs, which are used to treat both MDR as well
as XDR strains (Table 1). The overall alteration of responses
(resistance and susceptibility) of antitubercular drugs after biofield
treatment against XDR strains of
Mycobacterium
with respect to
control are presented in Figure 1.
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential
Using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189. doi:10.4172/2161-1068.1000189
Page 2 of 5
Mycobact Dis
ISSN:2161-1068 MDTL, an open access journal Volume 5 • Issue 4 • 1000189
S. No.
Antimycobacterial Agent
Group I
XDR Strain MDR Strain
MGIT (Control) MGIT (Treated) MGIT (Control) MGIT (Treated)
%R %S %I %R %S %I %NG %R %S %R %S
First Generation
1 Isoniazide (H) 100 0 0 73.33 0 0 26.67 100 0 100 0
2 Rifampicin (R) 96.67 0 3.33 70 3.33 0 26.67 100 0 100 0
3 Pyrazinamide (Z) 73.33 26.67 0 50.33 20 0 26.67 44.44 11.11 55.5 44.44
4 Ethambutol (E) 100 0 0 66.67 3.33 3.33 26.67 66.66 66.66 100 0
5 Streptomycin (S) 96.67 3.33 0 56.67 16.67 0 26.67 66.66 33.33 77.7 22.22
Second Generation
6 Kanamycin (K) 100 0 0 53.33 20 0 26.67 0 100 0 100
7 Ethionamide (Et) 76.67 20 3.33 53.33 20 0 26.67 22.22 77.77 33.3 66.66
8 P-amino salicylates (P) 43.33 56.67 0 20 53.33 0 26.67 0 100 0 100
9 Ofloxacin (O) 100 0 0 73.33 0 0 26.67 44.44 55.55 33.3 66.66
Third Generation
10 Moxifloxacin (M) 36.67 60 3.33 43.33 30 0 26.67 0 - 0 -
11 Amikacin (A) 80 20 0 46.67 26.67 0 26.67 0 - 0 -
12 Clofazimine (Cl) 13.33 86.67 0 3.33 70 0 26.67 0 - 0 -
13 Capreomycin (Ca) 20 80 0 36.67 36.67 0 26.67 1 - 1 -
Table 1: Percentage responsiveness of antitubercular drugs in extensive drug resistance (XDR) and multi drug resistance (MDR) strains of lab
isolates. All the values were expressed as percentage of isolates. R: Resistance; S: Susceptibility; I: Intermediate; NG: No Growth. Doses: H: 0.1
µg/ml; R: 2 µg/ml; Z: 100 µg/ml; E: 2.5 µg/ml; S: 2 µg/ml; K: 5 µg/ml; Et: 5 µg/ml; P: 4 µg/ml; O: 2 µg/ml; M: 1 µg/ml; A: 1 µg/ml; Cl: 0.5 µg/ml; Ca:
1.25 µg/ml; ‘-‘ Not reported; MGIT: Mycobacteria Growth Indicator Tube System.
Based on existing literatures isoniazide resistance developed due to
loss of catalase activity [19], transformation of functional katG gene,
deletions and mutation of others gene such as InhA [16]. In this
experiment after the biofield treatment resistance property was
reduced 26.7% in isoniazide as compared to control; it could be due to
some interaction at genetic level. Mono-resistance to isoniazid is quite
common and rifampin is rare. Rifampin or rifampicin resistance
occurs in
Mycobacterium
strains that are also resistant to isoniazid;
thus, rifampin resistance can be a surrogate marker for MDR [17].
More than 96% of the rifampin-resistant in
Mycobacterium
strains is
due to mutation of gene encoded in β subunit of DNA dependent
RNA polymerase (rpoB) [21,22]. The study results showed that
rifampicin improved susceptibility by three folds and simultaneously,
reduced 27.6% resistance properties as compared to control in XDR
strains (Table 1). Pyrazinamide is a prodrug that converted to
pyrazinoic acid by the enzyme pyrazinamidase, encoded by the pncA
gene in
Mycobacterium
strains. Mutations in pncA results to lost or
reduced pyrazinamidase activity which leads to development of
resistance [23]. In this experiment, after biofield treatment
pyrazinamide reduced 31.4% resistance property in XDR strains
besides increased susceptibility by four folds in case of MDR strains as
compared to control (Table 1).
The main target of ethambutol was mycobacterial embCAB operon
that is responsible for cell wall synthesis. Due to mutation of embCAB
lead to resistance against ethambutol in
Mycobacterium tuberculosis
,
but the exact molecular mechanism of resistance in non-tuberculous
mycobacteria is still unknown [24]. In this report, after biofield
treatment to the XDR strains, ethambutol decreased 33.33% resistance
and simultaneously improved sensitivity about three folds with respect
to control in XDR. In case of treated MDR strains, resistance property
increased by 50% and susceptibility was reduced sixty six folds with
respect to control (Table 1). Resistance of streptomycin was occurred
due to mutations in the gene encodes to the ribosomal protein S12,
rpsL [25]. This study results showed that streptomycin susceptibility
was enhanced about five folds and concurrently reduced 41.38%
resistance in biofield treated XDR strains as compared to control. In
MDR strains, resistance property slightly increased and sensitivity was
reduced in biofield treated groups as compared to control.
The resistant properties of all the second generation antitubercular
drugs such as kanamycin, ethionamide, p-amino salicylates and
ofloxacin of XDR strains of
Mycobacterium
were reduced by 46.67,
30.44, 53.84 and 26.67%, respectively as compared to control. Among
them only kanamycin showed an improvement in susceptibility by
Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential
Using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189. doi:10.4172/2161-1068.1000189
Page 3 of 5
Mycobact Dis
ISSN:2161-1068 MDTL, an open access journal Volume 5 • Issue 4 • 1000189
twenty folds towards treated XDR strains as compared to control.
Ofloxacin improved susceptibility 25.07% and simultaneously reduced
resistance also by 25% in treated group of MDR strain as compared to
control (Table 1).
S. No. Antimicrobial Agent Group II
MGIT
(Control)
MGIT
(Treated)
1Linezolid 8 2
2Clarithromycin 16 32
3Amikacin <1.0 <1.0
4Cefoxitin 256 256
5Cefriaxone >64.0 >64.0
6Imipenem 16 16
7Minocycline >0.25 <0.25
8Tobramycin 2 1
9Ciprofloxacin 0.25 <0.12
10 Gatifloxacin 0.25 0.12
11 Amoxicillin/Clavulanic acid 16 / 8 16 / 8
12 Trimethoprim/Sulfamethoxazole 0.12/2.4 <0.12/2.4
Table 2: Minimum inhibitory concentration (MIC) of antimicrobials
against ATCC strains of
Mycobacterium smegmatis
after biofield
treatment as per CLSI guidelines. MGIT: Mycobacteria Growth
Indicator Tube System; CLSI: Clinical and Laboratory Standards
Institute; MIC values are presented as µg/ml.
Apart from first and second generations antitubercular drugs
fluoroquinolones also possess antitubercular activity and it is well
accepted by the scientific community. Due to its wide distribution
pattern, can easily invade to an intracellular compartment of
Mycobacterium
and produce strong effects like macrophages without
cross-resistance to antitubercular drugs [26-28]. Among them,
moxifloxacin resistance to MDR-TB is very low [29] and highly
effective to isoniazide and rifampicin resistance cases. The main
targets of its bactericidal actions to DNA gyrase and topoisomerase IV
[30]. This study further indicated that there was a slight reduction of
MIC value (0.25-0.12 µg/ml) of gatifloxacin which is similar to
moxifloxacin after biofield treatment in
Mycobacterium smegmatis
(Table 2). According to George et al. amikacin and kanamycin are
highly resistance to
Mycobacterium
[31]. The study results also
demonstrated approximately 50% reduction of resistance in both
amikacin and kanamycin was found after biofield treatment in XDR
strains as compared to control. Besides, the sensitivity potential of
amikacin was improved by 33.35% in the treated group as compared to
control in XDR strains of
Mycobacterium
. Extensive study reports
demonstrate that clofazimine is median resistant to MDR-TB [32].
Basically, it is designed to treat leprosy, but in MDR-TB cases it is
extensively used as an alternative to TB regimens. This study results
showed that about 75% resistance property of clofazimine was reduced
in XDR-TB after biofield treatment as compared to control.
Capreomycin is an important and preferable drug in MDR-TB against
Mycobacterium smegmatis
. Resistance occurs due to mutation of tlyA
gene [33]. In eight lab isolates out of 30 XDR strains did not show any
growth of
Mycobacterium tuberculosis
after biofield treatment (Table
1). Biofield treatment might be responsible to do an alteration in
microorganism at genetic and/or enzymatic level, which may act on
receptor protein. While altering receptor protein, ligand-receptor/
protein interactions may alter that could lead to show different
phenotypic characteristics [34]. Based on these data, it is tempting to
speculate that certain alteration in terms of antimycobacterial
susceptibility happened due to biofield treatment.
Conclusions
Overall, the results reported here demonstrate that the biofield
treatment altered the resistance property in abundance number of
routinely recommended antitubercular drugs against XDR strains of
Mycobacterium
species. It has also been observed that there were a few
alterations in potency of selected antimicrobials in terms of MIC
values against XDR strains of
Mycobacterium
. It is assumed that Mr.
Trivedi’s biofield treatment could be applied to improve the sensitivity
of antitubercular drugs and an alternative therapeutic approach
against resistance strains of
Mycobacterium
.
Acknowledgement
The authors gratefully acknowledge to Trivedi science, Trivedi
testimonials, Trivedi master wellness and the whole team of PD
Hinduja National Hospital and MRC, Mumbai, Microbiology Lab for
their support.
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Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential
Using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189. doi:10.4172/2161-1068.1000189
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Citation: Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An Impact of Biofield Treatment: Antimycobacterial Susceptibility Potential
Using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189. doi:10.4172/2161-1068.1000189
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Mycobact Dis
ISSN:2161-1068 MDTL, an open access journal Volume 5 • Issue 4 • 1000189
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... Oxidative stress is one of the major factors along with other such as alcohol intake, air pollutions, environmental pollutants, physical stress, and radiations, etc. are some of the extrinsic factors, which results in generation of free radicals causing damage to body cells [5,6]. Thus properties [14][15][16], improved crop yield in agriculture science [17,18], microbiology [19][20][21], biotechnology [22,23], improved bioavailability of many compounds [24][25][26] ...
Article
The aim of the study was to evaluate the immunomodulatory activity of the Biofield Treated/Blessed proprietary test formulation consisting of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3) in male Sprague Dawley rats. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment/Blessing, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi remotely. Additionally, three group of animals were also received Biofield Energy Treatment per se (at day -15) under similar conditions. The parameters were assessed such as immune biomarkers (IgM, IgG, IgA, IgE, CD4+, CD8+, and CD28+), biochemistry and hematology and histopathology. The experimental results showed IgG level was significantly increased by 10.70% and 8.03% in the G6 (Biofield Energy Treatment per se at day -15) and G8 (Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15) groups, respectively as compared with untreated test formulation (G4). Additionally, CD8+ count was significantly increased by 20.67% in the G8 group, while CD28+ count was significantly increased by 11.70%, 8.32%, and 9.82% in the G7 (Biofield Energy Treated test formulation at day -15), G8, and G9 (Biofield Treatment per se (day -15) to animals plus untreated test formulation) groups, respectively after Biofield Energy Treatment to the animals as compared with the untreated test formulation. In hematological analysis, platelet count was increased in the G5, G6, G7, G8, and G9 groups by 40.69%, 27.95%, 26.67%, 38.58%, and 28.28%, respectively compared with the disease control (G2) group. Biochemical parameters showed significant decrease in the level of creatinine by 32.14% in the G9 group as compared with the G2 group. Further, animal body weight, feed intake, relative organ weight, and histopathological findings of all the tested groups did not show any abnormal findings with respect to the safe and non-toxic treatment strategies. Overall, the experimental data concluded that the Biofield Energy Treated/Blessed test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation has the significant capacity for immunomodulatory effect, stress management and anti-aging by improving overall health.
... The National Center of Complementary and Integrative Health (NCCIH) has been recognized and accepted Biofield Energy Healing as CAM health care approach in addition to other therapies, medicines and practices such as natural products, deep breathing, yoga, Tai Chi, Qi Gong, chiropractic/osteopathic manipulation, meditation, massage, special diets, homeopathy, progressive relaxation, guided imagery, acupressure, acupuncture, relaxation techniques, hypnotherapy, healing touch, movement therapy, Pilates, Rolfing structural integration, mindfulness, Ayurvedic medicine, traditional Chinese herbs and medicines, naturopathy, essential oils, aromatherapy, Reiki, cranial sacral therapy and applied prayer (as is common in all religions, like Christianity, Hinduism, Buddhism and Judaism) [21]. The Trivedi Effect ® -Biofield Energy Consciousness Healing Treatment has astonishingly ability to transform the characteristic properties of several organic compounds [22][23][24][25], pharmaceuticals [26,27], nutraceuticals [28], metals and ceramic [29,30], culture medium [31,32] and improve the overall productivity of agricultural crops [33,34], skin health [35,36], modulation in the efficacy of various living cells [37][38][39], and alteration of the isotopic abundance ratio in the organic compounds [40][41][42][43]. Thus, this study was designed to analyses the impact of Biofield Energy Treatment (The Trivedi Effect ® ) on the physicochemical, thermal, and spectroscopic properties of copper chloride by using various analytical techniques such as, powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), UV-visible, and FT-IR spectroscopy. ...
... Many scientific experiments were conducted to prove the impact of the Trivedi Effect ® -Consciousness Energy Healing Treatment on the non-living and living object(s). The Consciousness Energy Healing Treatment was proved with significant outcome in field of organic chemistry [11,12], material science [13,14], nutraceuticals [15,16], pharmaceutical sciences [17,18], cell biology [19,20], microbiology [21,22], and agriculture science [23,24]. Therefore, this study was designed to determine the impact of the Trivedi Effect ® -Consciousness Energy Healing Treatment on the physicochemical properties of V 2 O 5 powder using powder X-ray diffraction (PXRD) and particle size analysis (PSA). ...
... Similarly, the importance of the Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported by various researchers for its significant impact on the living organisms and non-living materials. The impact of the Biofield Energy Treatment has been significantly improved the agricultural productivity [20,21], antimicrobial activity [22][23][24], physicochemical properties of the pharmaceuticals/ nutraceuticals [25][26][27], various properties of metals, ceramics, and chemicals [28][29][30], and in the field of biotechnology [31,32], skin and bone health [33][34][35], and cancer research [36], etc. Thus, this research work was designed to determine the effect of the Biofield Energy Treatment (the Trivedi Effect ® ) on the physicochemical and thermal characteristics of magnesium by using various analytical techniques. ...
... This energy can be harnessed and transmitted by the practitioners into living and non-living things via the process of Biofield Energy Healing. The Biofield Energy Treatment, the Trivedi Effect ® , has been reported to have a significant impact in the field of cancer research [21,22], materials science [23,24], microbiology [25,26], agriculture [27,28], nutraceuticals [29,30], and biotechnology [31,32]. Further, the Trivedi Effect ® also significantly improved bioavailability of various low bioavailable compounds [33][34][35], an improved overall skin health [36,37], bone health [38][39][40], human health and wellness. ...
Research
Full-text available
The aim of this paper was to investigate the impact of the Biofield Energy Treatment on the test formulation by focusing on the function of vital organs viz. bones, heart, liver, lungs, and brain in various cell-based assays.
... The Trivedi Effect ® is a natural and methodically proven phenomenon in which an individual can harness this inherently intelligent energy and transfer it anywhere on the planet via the possible mediation of neutrinos [23]. The Consciousness Energy Healing Treatment has been reported for its remarkable effects in the field of biotechnology [24,25], agriculture science [26,27], human health and wellness [28][29][30], microbiology [31][32][33], livestock [34], and metals and ceramics [35,36]. The significant impact of the Trivedi Effect ® is also established scientifically on the physicochemical and thermal properties of various pharmaceutical/nutraceutical and organic compounds [37][38][39]. ...
... However, the National Center for Complementary and Alternative Medicine (NCCAM), well-defined Biofield Therapies in the subcategory of Energy Therapies [14]. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported to have created significant changes in the physicochemical properties of metals, chemicals, ceramics and polymers [15][16][17], improved agricultural crop yield, productivity, and quality [18,19],transformed antimicrobial characteristics [20][21][22], biotechnology [23,24], improved bioavailability [25][26][27], skin health [28,29], nutraceuticals [30,31], cancer research [32,33], bone health [34][35][36], human health and wellness. From outstanding benefits of Biofield Energy Treatment, the present study aimed to evaluate the impact of the Biofield Energy Treated (The Trivedi Effect ® ) DMEM on ED using the standard in vitro assay in Human Endothelial Hybrid Cell Line (EA. ...
... In this scenario, the Trivedi Effect ® -Consciousness Energy Healing Treatment has also been found to have a remarkable effect on the living organisms and non-living materials. The Biofield Energy Healing Treatment (the Trivedi Effect ® ) has been reported in various scientific studies regarding its significant outcome in the field of agriculture science [16,17], biotechnology [18], microbiology [19][20][21], skin health [22], livestock [23], cancer [24,25], and altering the properties of various pharmaceutical/nutraceutical compounds [26][27][28], and metals, ceramics, and polymers [29,30]. Hence, this study was designed to analyze the impact of the Biofield Energy Treatment on the physicochemical and thermal properties of flutamide with the help of analytical techniques such as, particle size analysis (PSA), powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). ...
... This energy can be harnessed and transmitted by the practitioners into living and non-living things via the process of Biofield Energy Healing. The Biofield Energy Treatment, the Trivedi Effect ® , has been reported to have a significant impact in the field of cancer research [21,22], materials science [23,24], microbiology [25,26], agriculture [27,28], nutraceuticals [29,30], and biotechnology [31,32]. Further, the Trivedi Effect ® also significantly improved bioavailability of various low bioavailable compounds [33][34][35], an improved overall skin health [36,37], bone health [38][39][40], human health and wellness. ...
Article
Full-text available
Dysfunction of vital organs are the main concern for human health. Therefore, it is necessary to homeostat the normal function of vital organs such as lungs, liver, brain, and heart for better health. The aim of this study was to evaluate the effect of the Consciousness Energy Treated test formulation on the function of vital organs such as bones, heart, liver, lungs, and brain in various cell-based assays. The test formulation and the cell media was divided into two parts; one untreated (UT) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Jagdish Singh, USA and were labeled as the Biofield Energy Treated (BT) test formulation/media. Cell viability data suggested that the tested formulation was safe and non-toxic in nature in six different cells. The experimental groups like untreated medium (UT-Med) + Biofield Treated Test Item (BT-TI), BT-Med + UT-TI, and BT-Med + BT-TI showed 190.5%, 56.3%, and 73.8% restoration of cell viability at 1µg/mL in human cardiac fibroblasts cells (HCF) compared to the UT-Med + UT-TI group. Moreover, BT-Med + UT-TI and BT-Med + BT-TI groups showed 98.8% and 79.1% restoration of cell viability at 10µg/mL, respectively in human hepatoma cells (HepG2) compared to untreated. Furthermore, 261.7%, 165.8%, and 167.8% restoration of cell viability was observed in adenocarcinomic human alveolar basal epithelial cells (A549) by BT-Med + BT-TI group at 0.1, 1, and 10µg/mL, respectively compared to untreated. Alkaline phosphatase (ALP) level was significantly increased by 52.5% and 66.1% in the BT-Med + UT-TI group at 10 and 50µg/mL, respectively in human bone osteosarcoma cells (MG-63) compared to the untreated. Additionally, the level of ALP was significantly increased by101% and 170.6%in the BT-Med + UT-TI and BT-Med + BT-TI groups, respectively at 50µg/mL in human endometrial adenocarcinoma cells (Ishikawa) compared to the untreated. The percent protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 191.8% (at 0.01µg/mL) and 141.8% (10µg/mL)in the UT-Med + BT-TI and BT-Med + BT-TI groups, respectively compared to the untreated in HCF cells. The percent protection of HepG2 (liver) cells (decreased of ALT activity) was significantly increased by 88.1% (at 1µg/mL) and 44.9% (at 63µg/mL) in the BT-Med + UT-TI and UT-Med + BT-TI groups, respectively compared to untreated in HepG2 cells. The percent protection of A549 (lungs) cells (increased of SOD activity) was significantly increased by 19.6% and 13.6% in the BT-Med + UT-TI and BT-Med + BT-TI groups, respectively at 25µg/mL compared to untreated in A549 cells. Serotonin level was significantly increased by 475.6% (at 0.1µg/mL), 311.9% (at 1µg/mL), 400.5% (at 10µg/mL) and 250.9% (at 63µg/mL) in the BT-Med + BT-TI group compared to the untreated in human neuroblastoma cells (SH-SY5Y). The relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 185% and 285.8% in the BT-Med + UT-TI and BT-Med + BT-TI groups, respectively at 10µg/mL compared to the untreated in MG-63 cells. Utterly, these results suggest that Biofield Energy Treated test formulation significantly improved the relevant bones, heart, liver, lungs, and brain-related functional enzyme biomarkers. Altogether data suggest that the Biofield Energy Treatment (The Trivedi Effect®) can be useful to protect and maintain the normal function of each vital organ such as lungs, liver, heart, brain, and bones. Therefore, The Trivedi Effect® can be used as a complementary and alternative therapy against several disorders such as coronary artery disease, heart attack, arrhythmias, heart failure, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, Wilson disease, pneumonia, asthma, emphysema, chronic bronchitis, cystic fibrosis, osteoporosis, etc.
... This energy can be harnessed and transmitted by the practitioners into living and non-living things via the process of Biofield Energy Healing. The Biofield Energy Treatment, the Trivedi Effect®, has been reported to have a significant impact in the field of cancer research [21,22], materials science [23,24], microbiology [25,26], agriculture [27,28], nutraceuticals [29,30], and biotechnology [31,32]. Further, the Trivedi Effect® also significantly improved bioavailability of various low bioavailable compounds [33][34][35], an improved overall skin health [36,37], bone health [38][39][40], human health and wellness. ...
Article
Full-text available
Vital organs dysfunctions are the major concern for human health worldwide. The study aim was to investigate the impact of Biofield Treated test formulation on vital organs function using cell-based assays. The test formulation/test item (TI) and cell media (Med) was divided into two parts; one untreated (UT) and other part received the Biofield Treatment remotely by a renowned Biofield Energy Healer, Victoria Lee Vannes, USA and was labeled as Biofield Treated (BT) test formulation/media. Based on the cell viability test formulation was found safe in six different cells. The test formulation groups showed 112.6% and 108.65% restoration of cell viability in human cardiac fibroblasts cells (HCF); while, 845.63% restoration of cell viability in human hepatoma cells (HepG2) compared to UT-Med + UT-TI group. Furthermore, 131.86% restoration of cell viability was observed in adenocarcinomic human alveolar basal epithelial cells (A549) than untreated. The alkaline phosphatase (ALP) level was significantly increased by 94.87%, 99.06%, and 105.13% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively at 10 µg/mL in human bone osteosarcoma cells (MG-63) than untreated. Additionally, ALP level was significantly increased by 150.97%, 382.08%, and 471.4% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively at 0.1 µg/mL in human endometrial adenocarcinoma cells (Ishikawa) than untreated. The percent protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 115.1% (1 µg/mL) and 165.77% (10 µg/mL)in BT-Med + UT-TI and BT-Med + BT-TI, respectively than untreated. The percent protection of HepG2 (liver) cells (decreased of ALT activity) was significantly increased by 117.65% (1 µg/mL) and 91.3% (63 µg/mL) in UT-Med + BT-TI and BT-Med + BT-TI, respectively than untreated. The percent protection of A549 (lungs) cells (increased of SOD activity) was significantly increased by 40.56% in UT-Med + BT-TI at 10 µg/mL than untreated. Serotonin level was significantly increased by 543.84% (1 µg/mL), 477.12% (10 µg/mL), and 457.22% (10 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively than untreated. The relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 186.96%, 341.43%, and 291.31% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively at 1 µg/mL than untreated. Overall, these results suggest that Biofield Treated test formulation significantly improved the bones, heart, liver, lungs, and brain-related functional enzyme biomarkers. Therefore, the Trivedi Effect® can be used as a complementary and alternative therapy against several disorders such as coronary artery disease, heart attack, arrhythmias, heart failure, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, Wilson disease, pneumonia, asthma, emphysema, chronic bronchitis, cystic fibrosis, osteoporosis, etc.
Article
Full-text available
Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The combination of biofield treatment along with administration of chemical additives demonstrated the best results with statistically increased yields and higher pest resistance in both test cropping systems. The specific mechanisms that lead to these preliminary results have yet to be determined.
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Full-text available
Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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This study tested the Null Hypothesis for the effect of BioField Energy applied to two separate crops under typical growing conditions, namely ginseng and organic blueberry in commercial plantings in Wisconsin and California, respectively. Following treatment to replicated plots in standard experimental design, data were collected at harvest for yield quantity and quality. Ginseng plants treated both pre-harvest and a combination of pre- and post-harvest showed market grade increases of 33.3% and 40.0%, respectively. Point of sale gross return for this crop is dependent upon tuber quality, and from these data the economics of these treatments were calculated. Based on stand adjusted yields and quality values, a combination of pre- and post-harvest treatment increased gross income by 57.4%. The second crop showed similar trends in positive responses. In the two blueberry varieties studied, Emerald treated plants showed 96% statistical increase in yield, while Jewel showed 31% increase. At the time of treatment, each variety was in a different stage of flowering. The Emerald variety was in the flowering stage, and Jewel was predominately in the fruiting stage. Both treated cultivars however demons-trated increased yield quantity and quality. The specific mechanisms that lead to these pre-liminary results need further investigation.
Article
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Purpose While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists. Results The results indicated that Mr.Trivedi’s energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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Breast cancer is one of the leading causes of cancer death among women of all races. Pain is a common symptom associated with cancer; 75–90% of cancer patients experience pain during their illness and up to 50% of that pain is undertreated. Unrelieved pain leads to increased levels of the stress hormone cortisol. The purpose of this study was to examine the impact of bioenergy on fecal cortisol levels for mice injected with murine mammary carcinoma 4T1in two separate pilot studies. Using a multiple experimental group design, six to eight week old female BALB/c mice were injected with tumor and randomly assigned, in groups of 10, to daily treatment, every other day treatment, and no treatment groups. Five days after tumor cell injection, bioenergy interventions were begun for a period of ten consecutive days. Fecal samples were collected for each study and ELISA analysis was conducted at the end of both studies. For both studies, cortisol levels were decreased in the every other day treatment groups but remained high in the no treatment groups. Future studies utilizing bioenergy therapies on cortisol levels in a murine breast cancer model can begin to describe pain outcomes and therapeutic dose.
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Tuberculosis, as known universally, is a chronic infectious disease that can affect any part of the body including mouth. It usually affects the lungs, TB bacilli can spread hematogenously to other parts of the body and this also includes mandible or maxilla. It can occur in the mouth involving the tongue with very unusual features and forms. So oral lesions, although rare, are very important for early diagnosis and interception of primary tuberculosis.
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Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The combination of biofield treatment along with administration of chemical additives demonstrated the best results with statistically increased yields and higher pest resistance in both test cropping systems. The specific mechanisms that lead to these preliminary results have yet to be determined.
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Lack of diagnosis and diagnostic capacity remain barriers to tuberculosis (TB) control. This review describes recent advances in TB diagnostics, the current TB diagnostic landscape, and some of the key challenges in the quest for accurate, simple diagnostic tests that have meaningful impact on the health of individual patients and on TB epidemiology.