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Nutritional efficacy of avocado seeds


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Screening of the aqueous extract of Tanzanian avocado seed of Persea specie was done for nutrients and phytochemical compounds of nutritional importance. The aim of the study was to test for the presence of nutrients (carbohydrate, protein and vitamin C) and other phytochemical compounds of nutritional and medicinal values in avocado seeds. Both dry and fresh avocado seeds were tested for presence of saponnin, flavonoids, tannin, carbohydrates, proteins and vitamin C. Tannins and flavonoids were indicated only in fresh avocado seeds but none in dry samples. Saponins were found in both dry and fresh seeds. Proteins, carbohydrates and Vitamin C were indicated in both fresh and dry seeds. The compounds are reported to be present in the edible part of avocado in different proportions. Interestingly, Tannin, a widely distributed compound in most unripe fruits, with the main role in protection of the fruit from predation, and perhaps as pesticides, was tested only in fresh seeds. Tannin is a water soluble compound and hence making the fresh and unripe fruits well protected against pests as well as predators. Furthermore, it was noted that the avocado seed is nutritionally valuable based on the phytochemical and nutrients it constitutes. Tannins that are said to be poisonous are normally present in fresh seeds and not in the dry seeds, hence recommended to dry the seeds before processing for consumption.
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Full Length Research Paper
Nutritional efficacy of avocado seeds
Leonia Ndesiamoo Henry*, Upendo Yonnah Mtaita and Catherine Clemence Kimaro
Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, P.O BOX 2958, Dar es
Salaam, Tanzania
Accepted 7 August, 2015
Screening of the aqueous extract of Tanzanian avocado seed of Persea specie was done for nutrients
and phytochemical compounds of nutritional importance. The aim of the study was to test for the
presence of nutrients (carbohydrate, protein and vitamin C) and other phytochemical compounds of
nutritional and medicinal values in avocado seeds. Both dry and fresh avocado seeds were tested for
presence of saponnin, flavonoids, tannin, carbohydrates, proteins and vitamin C. Tannins and
flavonoids were indicated only in fresh avocado seeds but none in dry samples. Saponins were found
in both dry and fresh seeds. Proteins, carbohydrates and Vitamin C were indicated in both fresh and dry
seeds. The compounds are reported to be present in the edible part of avocado in different proportions.
Interestingly, Tannin, a widely distributed compound in most unripe fruits, with the main role in
protection of the fruit from predation, and perhaps as pesticides, was tested only in fresh seeds. Tannin
is a water soluble compound and hence making the fresh and unripe fruits well protected against pests
as well as predators. Furthermore, it was noted that the avocado seed is nutritionally valuable based on
the phytochemical and nutrients it constitutes. Tannins that are said to be poisonous are normally
present in fresh seeds and not in the dry seeds, hence recommended to dry the seeds before
processing for consumption.
Key words: Avocado seed, carbohydrates, efficacy, flavonoids, nutrition, proteins, phytochemical compounds,
saponnins, tannins and Vitamin C
Avocados in tropical region and Tanzania in particular
are abundantly available despite the fact that they are
highly underutilized. The low popularity of the fruit
amongst consumers results in a lot of fruits going to
waste every time it is in season. The avocado fruit is
rich in nutrients, high in protein and vitamins. Studies
have shown that avocados are rich in macro nutrients
like carbohydrate, vitamin and proteins (Whitney &
Rolfes, 2005). Tanzanian avocado fruits vary from
round to pear-shaped with a long, slender neck, but
sometimes completely round shapes, and the color
ranges from green to dark purple. Avocados are one of
the great cancer fighting foods, rich in a multiplicity of
nutrients, including many potent anti-oxidants and
phytochemicals as well as vitamins, minerals, fiber and
monounsaturated healthy fats (Chen et al., 2008,
Ensinger, 1994., Kushi, et al., 2006). Dry avocado
seeds are used locally as a relief drink, in treating
diarrhea or dysentery, teeth aches as well as skin
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License 4.0 International License
ISSN: 2408-5472 Vol. 3 (5), pp. 192-196, August, 2015
Copyright ©2015 Global Journal of Food Science and Technology
Author(s) retain the copyright of this article.
Glob. J. Food Sci. Technol. 193
Consumption of avocado fruits in Tanzania communities
is very wide. This is due to the availability of avocado
fruits in Tanzania. A good number of people eat avocado
due to its taste, documented medicinal and nutritional
values and its cheap availability in the region. Apparently,
the commonly consumed part of avocado remains to be
the edible part i.e. the soft part, the layer between the
skin and the seed. The seed part of the fruit is commonly
not consumed due to its poor taste, little information on
its medicinal/nutritional value and also difficult processing
to taste. Therefore, different people never prefer the
avocado seeds mainly because they are not informed of
the nutrients and phytochemicals compositions in the
seed part.
The study aimed to establish the baseline on the
nutrients and phytochemical composition in the seed part
and hence recommend on the best exploitation of the
avocado seeds. The main objective of the study is
therefore to test for the presence of nutrients
(carbohydrate, protein, and vitamin C) and other
chemicals of nutritional and medicinal values in fresh and
dry avocado seeds.
Study Area
The study was conducted in Dar es Salaam in which
avocados were bought from Kariakoo market and the
samples were analyzed at Dar es Salaam Institute of
Technology (DIT) Laboratory. Samples of avocado seeds
both fresh and dry were tested.
Sample Preparation and Analysis
Fresh ripe fruits were taken and then the first and second
layer was peeled (removed) to obtain the central hard
seeds. Ten (10) seeds were grinded fresh while another
ten (10) seeds grinded dry both giving a uniform
powders. Aliquots of 100gm powders were soaked in
200ml distilled water for 12 hours at room temperature to
form the aqueous extract solutions. The extracts were
filtered using filter paper and the filtrates were analysed
in the laboratory.
Phytochemical Screening
Phytochemical screening of the extracts was carried out
to test for the presence of saponnins, tannins and
flavonoids. Chemical tests were carried out on the
aqueous extracts to test for the presence of
phytochemicals in the avocado seeds of both fresh and
dry fruits. Tannins were tested by taking about 2g of the
both fresh and dry powders, boiled in 20 ml of water in a
beaker and then filtered. Few drops of 0.1% ferric
chloride were added. The formation of brownish green or
blue black coloration indicated presence of tannin in the
seeds. A portion of 10ml of the filtrate were mixed with
5ml of distilled water and shaken vigorously for persistent
froth. The froth was mixed with 3 drops of olive oil and
shaken vigorously; the presence of saponins was
indicated by the formation of emulsion. Flavonoids were
tested by adding 5ml of dilute ammonia solution(NH4OH)
to a 5ml of portion of aqueous filtrate, then 1ml of
concentrated sulphuric acid (H2SO4) from which the
formation of yellow coloration indicated presence of
Nutrients Screening
Carbohydrates were tested as both reducing and non
reducing sugars. Benedict‟s solution was used to test for
reducing sugars. A 2.5g of seed powder were soaked in
45ml hot distilled water and cold distilled water added to
make a total of 50ml extract. Additions of cold distilled
water lead to color change to milky color indicating
presence of carbohydrates. Aliquots of 10mls were taken
from each flask into two beakers; 5mls of copper reagent
were added in each beaker, the mixture boiled for two
minutes. The change of colour from blue to brick red
indicated the presence of carbohydrates.
The reaction involves reduction of Cu2+ to Cu+
(precipitated as Cuprous Oxide) indicating the presence
of reducing sugars. Potassium iodide was added followed
with vigorous shaking; again the colour change from brick
red to light blue confirms the presence of carbohydrates
as polysaccharides. Addition of 5ml of normal 0.1M HCl
changed the colour to brick red confirming the presence
of reducing sugars as ketoses.
Aliquots of five (5) drops of millon‟s reagents were added
to 1ml of the extract in a test tube followed by 3 drops of
NaOH(aq). White precipitates were observed, which turns
red on heating, indicating presence of proteins.
Vitamin C
Portions of 10ml of the extracts were measured into test
tubes followed by addition of 2-3 drops of starch solution
as an indicator and few drops of iodine solution as
potassium iodide (KI) were added in the test tubes. In the
presence of Vitamin C (C6H8O6), Iodine is oxidized to
Iodide. The more iodine added to the vitamin C solution
more iodide is formed until all vitamin C had reacted to
Henry et al. 194
form dihydroascorbic acid (C6H6O6). Thereafter, more
addition of iodine increased its concentration in the
solution hence changing the starch to blue-black colour.
Therefore, the immediate change of the starch to blue-
black colour when Iodine was added indicates that
Vitamin C is absent.
C6H8O6 + I2 + H2O → 2I- + C6H6O6 + 2H+
Data Analysis
The test results were presented as positive (where the
tested component was indicated in the reaction) and/or
Negative (where the component could not be indicated).
Testing for Nutrients (Protein, Carbohydrate and
Vitamin C) in Avocado Seeds
The three nutrients were found to be present in the seed
extracts of both fresh and dry seeds. It was observed that
fresh seeds of avocado may contain more proteins than
the dry seeds based on the number of drops of the
millons reagent consumed per volume of the extract. The
general test for carbohydrates indicated presence of
carbohydrates in both fresh and dry seeds.
Carbohydrates are sugars that the body uses for energy.
Simple carbohydrates, also called simple sugars, provide
the body with quick energy. Vitamin C was present in
both samples though at small amount. Addition of iodine
solution to 10ml extracts of both fresh and dry seeds
containing starch indicator was performed. Few drops of
iodine were furnished without changing of the colour of
the starch. The end point was shown by a colorless
solution indicating that all ascorbic acid (Vitamin C) had
reacted. More addition of the iodine resulted to change in
colour of the solution to blue-black solution indicating a
complete consumption of the vitamin C (Iodine reacting
with the starch).
Table 1: Screening results for nutrients and
photochemical compounds of both fresh and dry
avocado seeds.
Fresh seed
Vitamin C
Testing for Tannins, Saponins, Flavanoids in
Avocado Seed.
Phytochemical screening of the seeds showed presence
of saponins in both fresh and dry samples whereas;
tannins were detected in fresh seeds but not in dry seed.
Flavonoids were present in fresh sample but not in dry
The Composition of Nutrients (Carbohydrate, Protein
and Vitamin C) in Avocado Seeds
Proteins, carbohydrates and vitamin C were all present in
both the dry and fresh seed samples. Studies have
shown that avocados are rich in macro nutrients like
carbohydrate, vitamin and proteins (Whitney & Rolfes,
2005). Carbohydrates exist in varying amounts in the
avocado seeds depending on the different activities that
took place in the fruit during storage process before the
analysis. During storage, fruits lose weight, shrivel and
change colour, lose acidity and ascorbic acid but gain
sweetness (Pareek & Dhaka, 2008, Pareek et al., 2009).
Also during this period, the enzyme activity, sugar and
carotenoid contents increase with corresponding
decrease in acidity, pectin and tannin content. To
compare the carbohydrates in both fresh and dry seeds it
needs a test that is specific for a specific type of
carbohydrates. The study by Flitsch & Rein, (2003),
explains the vital functions of carbohydrate found in
avocado seeds as supplying energy for the body process.
Some of the carbohydrates are immediately utilized by
the tissues and the remaining is stored as glycogen in the
liver and muscles and some are stored as adipose
tissues for future energy needs (Flitsch & Rein, 2003).
The proteins were also indicated in avocado seeds
though in varying amounts. Protein is a large biological
molecules consisting of one or more chains of amino
acids. The functions of proteins in the body include
replicating DNA and strengthen the blood vessels
(Gulteridge & Thornton, 2005). Like other biological
macromolecules such as polysaccharides and nucleic
acids, proteins form essential parts of organisms and
participate in virtually every process within cells. Many
proteins found in avocados catalyze biochemical
reactions and are vital to metabolism (Gulteridge &
Thornton, 2005).
Vitamin C constitutes a group of micro nutrients
commonly found in the seeds of certain species like
avocado (Ensminger, 2010). The presence of small
amounts of vitamin C in both fresh and dry samples may
be related to the effect of storage temperature of the
seeds and preservation method. The low storage
temperatures maintain relatively high Vitamin C content
Glob. J. Food Sci. Technol. 195
compared to fruits stored at intermediate and ambient
storage temperatures (Lee and Kader, 2000). Water loss
has also been documented to have a greater effect on
vitamin C levels than temperature (Nunes et al. 1998).
Apparently, Vitamin C is among the micro nutrients those
required in very small amounts for the functions of the
body. Vitamin C (ascorbic acid) is one of the most
important antioxidants. Blood levels of vitamin C are used
to measure nutritional, immune, and cardiovascular
status. Low values occur in scurvy, malabsorption
syndromes, inflammatory bowel disease, alcoholism,
pregnancy, hyperthyroidism, and kidney failure. The
study showed that Vitamin C is obtainable at higher
amounts in the fresh seeds than the dry ones. This is well
in agreement to the argument that higher temperatures of
storage and consequent water losses have an impact in
the stability of Vitamin C (Nunes et al. 1998). Ascorbic
acid is easily oxidised to dehydroascorbic acid in the
Iodine test. Complete reaction is shown by intense blue
colour complex indicating the presence of unreduced
Iodine (I2) in the solution.
Dihydroascorbic acid
The Composition of Tannins, Saponins and
Flavonoids in Avocado Seed
The presences of saponin in avocado seed make this
fruit more important because saponins have both
hypertensive and cardiac depressant properties. Saponin
binds to cholesterol to form insoluble complexes; dietary
saponins in the gut of monogastric combine with
endogenous cholesterol excreted via the bile. This
prevents cholesterol reabsorption and resulting to
reduction of serum cholesterol. Tannins are polyphenols
of high molecular weight which are water soluble and
capable of precipitating proteins (Bryant et al., 1992;
Kraus, at al., 2003). Tannin because of their binding
properties, are known to be strongly astringent. This
astringency appears to be major cause of reduced food
intake in mammals. There is some controversy, however,
over whether reduced food intake is the result of the toxic
nature of tannins or not (Hagerman et al, 1992; Silanikore
et al., 2001). This has been very important in protecting
fruits from the attack by insects especially before
ripening. Tannins decreases with the time of ripening and
hence reduced to amounts that are harmless to
mammals. Tannins have high contributions in the
organoleptic properties. In the current study, tannins were
only detected in fresh sample.
The Nutritional Value of Avocado Seeds
The phytochemical compounds in avocado seeds are
responsible for color and organoleptic properties and also
for prevention and treatment of many health conditions,
including cancer, heart disease, diabetes, and high blood
pressure (Kush et al, 2006). There is some evidence that
certain phytochemicals may help prevent the formation of
potential carcinogens (substances that cause cancer),
block the action of carcinogens on their target organs or
tissues, or act on cells to suppress cancer development
(Kush et al, 2006). Tannins were found in fresh seeds
and not in dry ones. This is well indicated even in unripe
fruits causing the bitter taste of the fruits. Tannins protect
fruits from predation, and perhaps also as pesticides, and
in plant growth regulation (Cheeke, et al, 2006). Likewise,
the taste or smell from the tannins is what causes the dry
and the formation of small purse-like gathering (puckery)
feeling in the mouth following the consumption of
unripened fruit of avocado. The seed of the avocado
contains a concentrate of tannins, and these makes the
taste extremely bitter. Tannins is an acid commonly found
in some drinks (e.g. wine, coffee, tea.); but in some cases
it can be the cause for an allergic reaction. In the seeds
tannins were found to concentrate in fresh seeds and
upon drying it disappears. Avocado seeds are high in
tannins, which you may be familiar with from wine, which
give them a somewhat bitter taste and red color. Despite
the taste, tannins are present in seeds at reasonable
levels which are harmless to consumers. The presence of
Ascorbic Acid
(Vitamin C)
Henry et al. 196
tannins in the body can improve the appetite and reduce
respiratory problem and circulatory disorder like lowering
blood pressure and reduce of cholesterol in blood
(Cheeke, et al, 2006). Saponins are naturally occurring
compounds that are widely distributed in all cells of
avocado plants. Saponins, which derive their name from
their ability to form stable, soap-like foams in aqueous
solutions, constitute a complex and chemically diverse
group of compounds (Shi et,al., 2004). Saponins affect
the immune system in ways that help to protect the
human body against cancers, and also lower cholesterol
levels (Shi et,al., 2004).
The flavonoids, have diverse beneficial biochemical
and antioxidant effects (Donald & Miranda, 2000). Their
dietary intake is quite high compared to other dietary
antioxidants like vitamins C. The antioxidant activity of
flavonoids depends on their molecular structure.
Flavonoids are different from other phytochemical
compounds especially on its reactivity nature. The other
compounds have the acidic groups that have binding and
nutralizable groups that are lacking in the flavonoids.
Antioxidants are compounds that protect cells against the
damaging effects of reactive oxygen species, such as
singlet oxygen, superoxide, peroxyl radicals, hydroxyl
radicals and peroxynitrite (Donald & Miranda, 2000). An
imbalance between antioxidants and reactive oxygen
species results in oxidative stress, leading to cellular
damage. The flavonoids have aroused considerable
interest recently because of their potential beneficial
effects on human health-they have been reported to have
antiviral, anti-allergic, ant platelet, anti-inflammatory, and
antitumor and antioxidant activities (Donald & Miranda,
Avocado seed contains a diverse number of nutrients and
phytochemical compounds of nutritional value.
Biologically, the seed part of avocado is meant for
reproduction purposes. Apparently, most of the seeds are
discarded harvazardly compounding the problem of
dangerous wastes especially in the cities. The study
shows that almost all the nutrients and phytochemicals
found in the soft edible part are also found in the seed
part though in varying concentrations. It is learnt that the
seed part is also a potential source of nutrients and
medicinal phytochemical compounds. Avocado seeds
prepared in different ways can be used to keep the body
fit from many diseases and hence reducing expenses for
treatments. The dry seeds are preferred over the fresh
ones for their easy processing and also the reduced
amount of tannins in their product. On the environmental
point of view, utilization of avocado seeds in this way
shall reduce a lot of solid wastes in the environment and
hence keeping our cities cleaner.
The maximum exploitation of the avocado shall require
further studies especially in quantifying the nutrients and
phytochemical compounds in both the seeds and the soft
edible part of the avocado.
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... The other polyphenol compounds found in avocado seeds are 3-O-caffeoylquinic acid, 3-O-p-coumaroylquinic acid, procyanidin trimer A(I), procyanidin trimer A(II), catechin, or epicatechin gallate [11]. Other research stated that avocado seeds have tannins, saponins, and flavonoids, which are parts of polyphenols [12]. Different varieties of avocado produce different content of phenolic compounds [13]. ...
... The presence of saponins, tannins, and flavonoids in avocado seed was tested using 0.1% ferric chloride added with a few drops in filtered, boiled water of avocado seed [12]. Indication of tannins showed by blue-black or brownish-green colour. ...
... It can prevent lipid oxidation and reduce the risk of inflammatory diseases [19]. Henry et al. (2015) claimed that saponins have hypertensive activity and cardiac depressant because cholesterols are bound with saponins and form insoluble complexes. Saponins in the human body can affect the immune system, help against cancer, and lower cholesterol levels. ...
Full-text available
Persea americana Mill. seed or avocado seed contains phytochemical components such as flavonoids, tannins, saponins, phenolic, and alkaloid. The phenolic compounds of avocado seed are mainly catechin, hydroxybenzoic acid, caffeic acid, chlorogenic acid, coumaric acid, ferulic acid, and triterpenoid glycosides. This review also discusses various extraction methods using an organic solvent, inorganic solvent, and a mixture of solvents. The analysis methods to measure the chemical properties of extracted phytochemicals from avocado seed are discussed. The functional properties of the phytochemicals obtained from the seed are presented.
... The benefits of avocado seeds include starch content up to 74.47% and antioxidant minerals like Fe, Mg, Zn, Vitamin C, Vitamin E, and fatty acid (Orhevba et al., 2011;Vinha et al., 2013;Aliakbarzadeh et al., 2016). Compounds like phenolic, tannin, saponin, flavonoid, Vitamin C, and Vitamin E in avocado seeds can reduce triacylglycerol, total cholesterol, and LDL-C (Low Density Lipoprotein-Cholesterol) levels because they protect the mucous membranes from lipid peroxide and prevents free radicals (Henry et al., 2015;Shehata and Soltan, 2013;Vinha et al., 2013). Lipase enzymes in avocado seeds are perceived to play a role in triglycerides degradation in the bloodstream, thereby reducing triglyceride levels. ...
... Flavonoids, tannins, and saponins in avocado seeds can inhibit the production of cholesterol in the liver synthesized by the ACAT (acyl-CoAcholesterol acyltransferase) enzyme (Henry et al., 2015;Shehata and Soltan, 2013). Cholesterol in the liver will then be sent via VLDL lipoprotein to the body tissues, including the formation of egg follicles; therefore, the decreased blood cholesterol is followed by decreased LDL and triglycerides and increased HDL of quail blood. ...
... Similarly, Uchenna et al. (2017) reported that avocado seeds flour up to 4% can reduce the blood cholesterol in rats. According to Henry et al. (2015) and Shehata and Soltan (2013), flavonoids in avocado seeds can lower triacylgliserol, total cholesterol, and LDL-C (Low Density Lipoprotein-Cholesterol) by protecting against lipid peroxide and preventing free radicals. This research found that avocado seed flour contained 5.41% flavonoids, which is perceived to affect the decreased quail blood cholesterol levels in quails. ...
Full-text available
The purpose of this research is to determine the effect of avocado seed flour (ASF) supplementation in feed on blood lipids and quail egg yolk cholesterol. The materials were 100 female quails aged 4 weeks old with ration ingredients consist of corn, bran, paddy, soybean meal, fish flour, avocado seed flour, palm oil, CaCO3, premix, lysine, and methionine. The research conducted an experiment with 4 treatments of ASF supplementation levels incorporated into basal feed, namely 0% ASF (R0), 3% ASF (R1), 6% ASF (R2), and 9% ASF (R3). The observed variables included blood cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), triglyceride, and egg yolk cholesterol. The data were subjected to the one-way Analysis of variance (ANOVA) in a completely randomized design, followed by HSD test when differences between treatments were observed. The result showed that ASF supplementation significantly reduced blood cholesterol levels and the egg yolk cholesterol, did not significantly affect LDL and triglyceride levels. The HDL levels and egg yolk cholesterol tend to decrease as the level of ASF increased. It is concluded that avocado seed flour (ASF) supplementation up to 9% is safe for quail feed supplementation.
... Avocado seeds equally possess insecticidal, fungicidal, and antimicrobial activities [15]. Study shows that P. americana contains carbohydrates, proteins, vitamins, and phytochemicals like saponins, phenolics, alkaloids, tannins, and flavonoids, which contributes to its high nutritional and pharmaceutical habits [16]. Helminthiasis is treated in the western region of Cameroon with the help of P. americana. ...
... Previous studies conducted by Henry et al. [16] have shown that P. americana seed extracts contain lipid substances, alkaloids, phenols, and hydro-soluble compounds. From the analysis of the different IC 50 , it is clear that the aqueous extract is more active for the inhibition of hatching at 95% confidence interval. ...
Full-text available
Background Infections with gastrointestinal helminths constitute a serious obstacle to the good health of the local population in most African Countries. The aim of this study was to evaluate the anthelminthic activity of Persea americana ethanol and aqueous extracts against Heligmosomoides polygyrus using the worm microtracker. Method Aqueous and ethanolic extracts of P. americana were prepared. Different concentrations of the extracts were tested against the egg and larvae stages of H. polygyrus using an automated high-throughput method. Briefly, embryonated eggs and larvae of this parasite were obtained after the incubation of fresh eggs at 25°C for 24, 48, and 96 hours for embryonated eggs, L1 and L2 larvae, respectively. One hundred microliters of the plant extracts at various concentrations were put in contact in a 96-well microplate with a suspension of 100 embryonated eggs in a total volume of 200 μL and incubated in a worm microtracker where the motility of the worms was recorded every 30 minutes for the ovicidal activity. The final tested extract concentration was 5, 2.5, 1.25, 0.625, and 0.3125 mg/mL, whereas ringer solution (0.95%) and 1.5% Dimethyl sulfoxide (DMSO) were used as negative controls and levamisole as positive control. The same method was used for larvicidal activities. The anthelmintic activity was determined using the average movement of the worms in the tested product compared with the negative control (1.5% DMSO and ringer solution). Results The egg hatching rates of H. polygyrus had IC50 of 0.49 mg/mL (95% confidence interval: 71.70–92.03) and 0.22 mg/mL (95% confidence interval: 74.28–86.18) for the ethanol and aqueous extract, respectively. These IC50 indicate that the aqueous extract is more active for the inhibition of hatching at a 95% confidence interval. The aqueous and ethanol extracts presented mean inhibitory hatching rates of 78.33 ± 1.67% and 75.67 ± 1.15% at 5 mg/mL, respectively, with no significant differences. The highest percentage of inhibition of L1 larva was observed at 5 mg/mL with 89 ± 2.3%and 85 ± 2.7% for the ethanol and aqueous extracts, respectively. The lowest percentage of inhibition was observed at 0.3125 mg/mL, with 54.67 ± 3.38% and 49 ± 2.64% for the ethanol and aqueous extract, respectively. No significant differences were observed between the two extracts at 5 mg/mL with an inhibitory percentage of 90.67 ± 3.05% (ethanol) and 89.33 ± 2.08% (aqueous). Conclusion Extracts of P. americana seeds possess nematocidal activity, however, further in silico and in vivo investigations are necessary to confirm their anthelminthic activity.
... The food industry applies the antioxidant properties of phytochemicals from avocado by-products to delay the lipid oxidative damage that aids food rancidity. These biological impacts of avocado by-products are majorly associated with flavanol derivatives and phenolic acids ( Henry et al., 2015 ;Merino et al., 2021 ). ...
... The study found that avocado seed starch has a potential use as a thickening, gelling, bioplastic, and an emulsifying agent in food systems as well as a possible ingredient in biodegradable polymers for food packaging Charles et al., 2022 ). In addition, avocado seed powder is also applicable locally in Tanzania as a relief drink in treating diarrhea or dysentery, teeth aches, and skin diseases ( Henry et al., 2015 ). ...
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Globally, about 8.06 million tonnes of avocado are produced annually. According to Food and Agricultural Oorganisation Corporate Statistical Database, 2020, Kenya was ranked the 6th highest avocado producer globally. The processing of avocado leads to the production of large quantities of peels and seeds that are typically disposed off as waste without any further application. Avocado peels and seeds are claimed to contain high amounts of phytochemicals that offer therapeutic and other nutritional benefits. These compounds can be used to produce economically high-value products. The amounts of phenols present vary with different factors such as the varieties of avocado, level of ripening, maturation, growing conditions, and climatic conditions. Studies have shown that phenolics found in avocado peels and seeds exhibit antioxidant, anti-inflammatory and antimicrobial properties and contain related health benefits. Despite findings, that these by-products contain high-value functional properties that are beneficial to human health, pharmaceutical and food industry, they still remain underutilized. Avocado seeds and peels are currently being used traditionally in the production of soaps, pharmaceuticals, and food products, including beverage products. This review, therefore, focuses on the avocado production trend in Kenya from the year 2011 to 2020 compared to other parts of the world, with emphasis on the composition of avocado seeds and peels and their utilization in food products.
... ASE is a precious source due to its rich nutritional and phenolic content [31], as was also demonstrated in this study. The total phenolic content of ASE was calculated to be 0.16 g GAE / g dry ASE. ...
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The electrospinning technique is particularly prominent in membrane production techniques due to its easy applicability and ability to obtain nanofibers in various diameters with high surface area. Titanium dioxide (TiO2) is often used in biomaterial technologies due to their high biocompatibility, high processability and their photocatalytic activities in waste water treatment. In this study, TTIP (Titanium tetra isopropoxide) was used as a TiO2 precursor and PVP polymer was used as a carrier polymer for electrospinning. Avocado seed extract (ASE) which is a new and valuable source of phenolic compounds was used for the coordination and reduction of TTIP. TiO2-PVP-Avocado seed extract (T/P/A) composite nanofibers were produced at different voltages, distances and polymer concentrations. Crystalline TiO2 formation was not observed in as-spun nanofibers, thus selected nanofibers were heat treated at 500oC for 3 h. Smooth and integrated TiO2 nanofibers prepared by using 5 w% PVP, at 15 kV and 15 cm distance with or without ASE were imaged by Scanning Electron Microscopy (SEM). X-ray Diffraction (XRD) patterns of heat-treated TiO2 nanofibers prepared in the presence of ASE were crystallized mainly in anatase form. However, both anatase and rutile phases were obtained in the crystalline structure of TiO2 nanofibers when ASE was not used. Specific IR vibrations of TiO2 were shown by Fourier Transform Infrared Spectroscopy (FTIR). These green synthesized electrospun TiO2 nanofibers may have potential to be used in biomaterial and engineering applications.
... Further, the antinutritional components could be removed or reduced significantly from the seeds by physical factors such as soaking for 24 hours or boiling for 25 minutes in water [3,18]. Some antinutrients (tannins and flavonoids) can be removed by drying the seeds prior to processing [19]. ...
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Consumption of avocado (Persea americana Mill.) has increased worldwide in recent years. The avocado pulp is used, but the peel and seed are discarded as waste. Studies have shown that the seeds are rich in phytochemicals that can be utilized in food systems. The objective of this study was to evaluate the potential of Hass avocado seed as a source of polyphenols in the processing of model beverages and baked products with functional properties. The proximate analysis of the avocado seed powder was carried out. The shelf life of phenols in avocado seed powder (ASP) stored in dark, amber-colored bottle and transparent bottle was studied for six months. The seed extract was incorporated into model beverages of different pHs stored at refrigerated and ambient temperatures, and the shelf life was monitored for 20 weeks. The seed powder was incorporated into baked products at 0, 15, 30, or 50% followed by total phenolic content and sensory property analysis. Proximate composition of the seed powder for moisture, ash, protein, fiber, fat, and total carbohydrates was 14.19, 1.82, 7.05, 4.00, 13.64, and 59.30 percent, respectively. During storage of the seed powder, there was no significant difference ( P > 0.05 ) in the phenol content under the different storage light conditions for six months. In the model beverages, lower pH levels (2.8, 3.8, and 4.8) and those stored at ambient temperatures (25°C) recorded lower phenol content than the control pH, i.e., 5.5, and those under refrigerated conditions throughout the storage period studied (20 weeks). The concentration of phenols in the baked products increased with increasing avocado seed powder. The color of all the queen cake formulations was liked very much by the sensory panel. The aroma of 0% and 15% ASP was liked very much, while the other formulations (30% and 50%) were liked moderately. The taste rating and overall acceptability decreased with an increasing avocado seed powder in the queen cake formulations. Avocado seed extracts can be incorporated to prepare functional beverages and functional baked products that are acceptable by sensory panelists.
... The use of waste avocado seeds soaked in filtrate of husk ash water can be used as an energy source of feed to reduce consumption of corn so as to reduce feed costs which in turn will increase profits [6]. utilization of avocado seeds in this way will reduce a lot of solid waste in the environment and hence keeping our city cleaner [7]. ...
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Feed is one of the important components in fish farming as a source of material and energy, feed is also the largest component (50-70%) which causes an increase in production costs. Therefore, to reduce feed production costs is to mix inexpensive and easily accessible natural feed with a high enough protein content. During the harvest season the avocado is abundant and avocado seeds are disposed of as waste. This study aims to determine the effect of a combination of avocado Perseaa mericana M. and pumpkin Cucurbita moschata D seed on the growth and feed efficiency of Osphronemus gouramy Lac. The design used non-factorial Completely Randomed Design consisting of 4 treatments and 6 replications: P0 (control feed), P1 (40 grams of avocado seed feed + 40 grams of pumpkin seeds + 20 grams of mixed ingredients), P2 (20 grams of avocado seed feed + pumpkin seeds 60 grams + 20 grams of mixed ingredients) and P3 (60 grams of avocado seed feed + 20 grams of pumpkin seeds + 20 grams of mixed ingredients). Data analysis used Analysis of variance and Duncan’s test using SAS 9.3.1 software. The results showed that giving a combination of avocado seeds and pumpkin seeds can increase the growth of gouramy. The highly effective growth was found in P2 feed of 11.86 grams with a growth rate of 2.69% / day, while low growth occurred in the P3 treatment of 9.46 grams with a growth rate of 2.11% per day. The proximate test of the test feed showed that the highest protein was found in P2 feed, namely 25.01%. The best feed efficiency was found in P2 feed which had 6.91% crude fiber, and the highest protein content in P2 was 18.36%. It concluded that there was an effect of a combination of avocado and pumpkin seed feed on the growth and feed efficiency of Gouramy. Avocado and pumpkin seeds supplementation can be used as ingredients fish feed.
... It is well-known that new therapeutic agents against oxidative stress are important as antioxidant substances and present in common foods such as avocado, which are widely distributed and consumed globally (Ademoyegun et al. 2016;Boadi et al. 2015;Dulay and De Castro 2016;Henry, Mtaita, and Kimaro 2015). Both Ademoyegun et al. (2016) and Dulay and De Castro (2016) demonstrated several beneficial substances in avocado including phenols and flavonoids which were detected predominantly in the seeds of the fruit. ...
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Oxidative stress is known to be involved in development of numerous diseases including cardiovascular, respiratory, renal, kidney and cancer. Thus, investigations that mimic oxidative stress in vitro may play an important role to find new strategies to control oxidative stress and subsequent consequences are important. Rotenone, widely used as a pesticide has been used as a model to simulate oxidative stress. However, this chemical was found to produce several diseases. Therefore, the aim of this study was to investigate the antioxidant and cytoprotective effect of avocado (Persea americana Mill) extract and oil in monkey kidney epithelial cells (VERO) exposed to rotenone. VERO cells were exposed to IC50 of rotenone in conjunction with different concentrations of avocado extract and oil (ranging from 1 to 1000 µg/ml), for 24 hr. Subsequently, cell viability and oxidative metabolism were assessed. Data demonstrated that avocado extract and oil in the presence of rotenone increased cellular viability at all tested concentrations compared to cells exposed only to rotenone. In addition, extract and avocado oil exhibited antioxidant action as evidenced by decreased levels of reactive oxygen species (ROS), superoxide ion, and lipid peroxidation, generated by rotenone. Further, avocado extract and oil appeared to be safe, since these compounds did not affect cell viability and or generate oxidative stress. Therefore, avocado appears to display a promising antioxidant potential by decreasing oxidative stress.
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Gummies are becoming more popular in the market as people become more aware of healthy midlife lifestyles. The goal of this study was to make gummies out of avocado and okra seeds. Because avocado and okra seeds are high in antioxidants and nutrients, adding them to this gummy supplement provides health benefits. The proximate composition, physicochemical properties, and antioxidant properties were not significantly different (p ≤ 0.05). The gummies had a proximate composition of moisture (36.39%), ash (0.13%), crude fat (0.21%), protein (0.58%), and carbohydrates (62.77%). The gummies' physicochemical characteristics included pH (3.2) and water activity (0.6). The percentage of antioxidant capacity varied from 15.60% to 42.55%. The organoleptic evaluation of the sample S1 gummies (co, texture, flavor, taste, and overall acceptability) revealed that they had the best organoleptic characteristics and received the highest score. The product was roughly stable in refrigerator condition for three months, according to studies done on samples stored at room temperature and in the fridge.
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Purpose of review: This article reviews studies conducted on maturity indices, climacteric behaviour, artificial ripening, and pre- and postharvest treatments, which affect the shelf-life, packaging, storage and postharvest pathology of ber fruits. Findings: Ber fruits show climacteric respiration behaviour. Various maturity indices including colour, total soluble solids, harvesting date, days from full bloom to maturity, etc, have been characterised. The postharvest quality and shelf-life of ber fruits are influenced by both pre- and postharvest factors; plant growth regulators, stage of maturity, composition of fruits, storage conditions, type of storage and packaging have been identified as important factors. Low storage temperatures (3–5°C) can extend the shelf-life beyond 2–3 weeks. Limitations: Ber is largely found in India and not much advanced research has been carried out on this fruit. To date standardised storage conditions are not available for commercial recommendations. Controlled and modified atmosphere use has not been investigated. Directions for further research: More attention should be focused on characterising the physiology of ber fruit, including ripening, gene expression, protein synthesis and regulation of enzymes catalysing sugar formation and translocation. Reliable maturity and international standards, with respect of quality, should be developed. Low temperature storage methods such as controlled and modified atmosphere should be studied and standardised, and ethylene management with 1-methylcyclopropene should be investigated.
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Tannins are a diverse group of compounds which precipitate protein. The impact of tannins on herbivory has been difficult to assess because of diversity in tannin chemistry and in animal physiology. We have evaluated the effects of tannin on large ruminants (deer, sheep) using artificial diets containing well-defined tannins, and have compared the results to those obtained with natural forages. The different effects of condensed tannins and gallotannins on herbivores are related to the chemical stability of the tannins. Commercial tannic acid does not have the same effects on herbivores as gallotannins in natural forages. Molecular weight apparently determines the metabolic fate of gallotannins from various sources.
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Tannins make up a significant portion of forest carbon pools and foliage and bark may contain up to 40% tannin. Like many other plant secondary compounds, tannins were believed to function primarily as herbivore deterrents. However, recent evidence casts doubts on their universal effectiveness against herbivory. Alternatively, tannins may play an important role in plant–plant and plant–litter–soil interactions. The convergent evolution of tannin-rich plant communities on highly acidic and infertile soils throughout the world, and the intraspecific variation in tannin concentrations along edaphic gradients suggests that tannins can affect nutrient cycles. This paper reviews nutrient dynamics in forest ecosystems in relation to tannins. Tannins comprise a complex class of organic compounds whose concentration and chemistry differ greatly both among and within plant species. Because the function and reactivity of tannins are strongly controlled by their chemical structure, the effects of tannins on forest ecosystem processes are expected to vary widely. Tannins can affect nutrient cycling by hindering decomposition rates, complexing proteins, inducing toxicity to microbial populations and inhibiting enzyme activities. As a result, tannins may reduce nutrient losses in infertile ecosystems and may alter N cycling to enhance the level of organic versus mineral N forms. The ecological consequences of elevated tannin levels may include allelopathic responses, changes in soil quality and reduced ecosystem productivity. These effects may alter or control successional pathways. While a great deal of research has addressed tannins and their role in nutrient dynamics, there are many facets of tannin biogeochemistry that are not known. This lack of information hinders a complete synthesis of tannin effects on forest ecosystem processes and nutrient cycling. Areas of study that would help clarify the role of tannins in forest ecosystems include improved characterization and quantification techniques, enhanced understanding of structure-activity relationships, investigation of the fate of tannins in soil, further determination of the influence of environmental factors on plant tannin production and decomposition, and additional information on the effects of tannins on soil organisms.
Although the physiological reason underlying deterrence by secondary metabolites is not well understood, the available evidence indicates that toxicity is more important than digestion inhibition. Resource limitation influences the production of secondary metabolites by woody plants. Species that are adapted to unproductive habitats are more chemically defended than species that are adapted to productive habitats. Resource limitation also affects the phenotypic expression of chemical defense with nutrient stress favoring increased production of carbon-based secondary metabolites and reduced production of nitrogen-containing secondary metabolites. Light stress has the opposite effects on the production of these substances. -from Authors
Synthetic polymers such as water-soluble polyvinyl pyrrolidone (PVP), water-insoluble polyvinyl polypyrrolidone (PVPP), and water-soluble polyethylene glycol (PEG) contain sufficient oxygen molecules in a chain to form strong hydrogen bonds with the phenolic and hydroxyl groups in tannins. This review deals with the practical uses of tannin-binding agents, particularly PEG, in tannins assays and for determining the negative effects of tannins on feed intake and digestion in ruminants. A gravimetric method to assay tannins by precipitation with PVPP is specific for tannins and does not require standards. The extractability of tannins from plant tissues can be reduced by drying samples at temperatures above 50°C and is dependent on many other factors, such as content and types of plant proteins. Therefore, it is not feasible to recommend a single, optimal protocol for all plant samples. A method to assay tannins which is based on measuring the amount of binding of PEG to plant samples was shown to be simple and accurate. It can also overcome some of these extractability problems. The following biological effects of tannins were investigated in studies where tannin anti-nutritional effects were partially or completely neutralised by varying levels of PEG supplementation. (1) Effects on appetite: the negative effects of tannins on appetite can occur in the short-term (within 20-60 min) and the long-term (days and weeks), Astringency and adverse postingestive influences of tannins on the epithelium of the oral cavity and the foregut cause short-term effects on food intake. Long-term effects can be related to reduction in the concentration of ammonia and volatile fatty acids (VFA) in rumen fluids, which can in turn serve as metabolic cues for deficiency of nitrogen (ammonia), energy (VFA), or both. (2) Effects on digestion: increasing content of tannins in foliage can be associated with an increase in bound protein and with reduced degradation rate of the degradable matter in the rumen, but there is no corresponding increase of the non-degradable fraction. Consequently, organic matter, protein, and cell wall digestibility are inversely related to tannin concentrations. (3) Inducing digestive responses: if a significant amount of tannins reach the duodenum, they may markedly reduce the intestinal activity of pancreatic enzymes (trypsin and amylase) and amino acids absorption from the intestine. Condensed tannins can also reduce the content of fluid and particulate matter in the rumen, accelerate the passage of liquid from the abomasum, and delay the passage of digesta in the intestine. The overall effect is a delay in the passage of fluid and particulate matter throughout the entire gastrointestinal tract. It is hypothesised that these responses are largely the consequence of the interaction of tannins with digestive enzymes and the epithelium lining the digestive tract.