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In Vitro Evaluation of Biofield Treatment on Cancer Biomarkers Involved in Endometrial and Prostate Cancer Cell Lines

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Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. This study aimed to investigate the effect of biofield treatment on cancer biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Trivedi’s biofield treatment, referred as treated. Control and treated samples were studied for the determination of cancer biomarkers such as multifunctional cytokines viz. interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), interleukin-2 receptor (IL-2R), prostate specific antigen (PSA), and free prostate specific antigen (FPSA) concentrations using ELISA assay on day 10. Experimental results showed a significant reduction of IL-6 level in endometrium (12%) and prostate (98.8%) cancer cell lines while a significant increase was observed in TNF-α level in endometrium (385%) and prostate (89.8%) cancer cell lines as compared to control. No alteration of PSA level was observed in biofield treated endometrium and prostate cell line. Similarly, no alterations were evident in IL-2R and FPSA levels in endometrium and prostate cell lines after biofield treatment as compared to control. In conclusion, results suggest that biofield treatment has shown significant alterations in the level of cytokines (IL-6 and TNF-α) in both endometrium and prostate cancer cell lines.
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Open Access
Research Article
Cancer
Science & Therapy
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ISSN: 1948-5956
Trivedi et al., J Cancer Sci Ther 2015, 7:8
http://dx.doi.org/10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 253
Keywords: Bioeld treatment; Cancer biomarker; ELISA; TNF-α;
IL-6; Prostate cancer; Endometrium cancer
Introduction
Cancer has the potential to invade or spread to other parts of the
body which involves abnormal cell growth. Endometrium cancer
(EC) is the most common type of uterine cancer which starts in the
endometrium. EC cases are increasing worldwide and even higher in
developing countries [1]. However, physicians have developed new
diagnostic techniques and treatments, which results in decreased
reported cases of cancer in developing countries [2]. Endometrioid
adenocarcinoma is the most common type of EC with clear cell
histologic appearance made up of cells in glands that look much like
the normal uterine lining (endometrium) [3]. e survival rate in an
early and advanced stage of diagnosed EC is less than ve years [4],
and chances of about 50% recurrence aer postoperative therapy is
still high [5]. Most common cancer in male, especially in the western
world causing death is prostate cancer (PC). In the United States,
approximately 238,590 new cases of PC with a death of about 29,720
cases [6]. Progression of PC is through a multi-step development such
as involvement of growth factor, cytokines, and hormones [7].
Role of immune cells estimation in microenvironment of tumor
has been well established. On the primary hormonal basis, these
immune cells in tumor respond to produce a wide range of cytokines,
chemokines, and growth factors. Interleukins (IL-1, IL-6, and IL-
8), tumor necrosis factor-α (TNF-α), vascular endothelial growth
factor (VEGF), granulocyte-macrophage colony stimulating factor
(GM-CSF), and colony stimulating factor-1 (CSF-1) are the common
inammatory markers used in diagnosis [8]. IL-6 and TNF-α, are
considered as the major cancer biomarkers of a network involved in
EC and PC development with multiple biological characteristics [9,10].
TNF-α, is a multifunctional cytokine which plays a major role in cell
death and survival, as well as in inammation and immunity. TNF
increases susceptibility to heat-induced cell death (apoptosis) in a time-
dependent manner, an important protective cellular activity. IL-6 is a
pleiotropic cytokine, which exerts inammatory and immune response
on various cell types including prostate carcinomas. Serum IL-6 is the
surrogate marker in the clinical stage of PC. High concentrations of
serum IL-6 are associated with an increased risk of death in patients with
cancer. Expression of IL-2R, PSA and FPSA are the robust biomarkers
in predicting a diagnosis assay for prostate cancer. Normally, IL-2R and
IL-6 concentrations were signicantly higher in prostate cancer tissue
than in normal tissue.
In the last 70 years, cancer treatment strategies have rapidly
increased. Chemotherapy always associated with attached side eects
such as hair loss, nausea, vomiting, cognitive dysfunction, change in
sexual function and adverse impact on patient’s quality of life [11].
Surgery is still the main treatment strategy for early-stage diagnosis of
cancer, but for advanced and reoccurrence cases multimodality therapies
like radiations and chemotherapy being frequently used [12,13].
Increased use of alternative integrative medicine approaches have been
recently introduced and used to promote wellness and minimize side
eects from cancer. In the year 2007, 40% U.S. population has used
natural products as a complementary and alternate medicine (CAM)
[14]. National Center for Complementary and Alternative Medicine
*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory
Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,
Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-6660006; E-mail:
publication@trivedisrl.com
Received June 24, 2015; Accepted July 30, 2015; Published August 03, 2015
Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro
Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in Endometrial
and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-
5956.1000358
Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited.
Abstract
Increasing cancer rates particularly in the developed world are associated with related lifestyle and
environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches
in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening
problems to promote human wellness. This study aimed to investigate the effect of bioeld treatment on cancer
biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in
two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Trivedi’s bioeld
treatment, referred as treated. Control and treated samples were studied for the determination of cancer biomarkers
such as multifunctional cytokines viz. interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), interleukin-2
receptor (IL-2R), prostate specic antigen (PSA), and free prostate specic antigen (FPSA) concentrations using
ELISA assay on day 10. Experimental results showed a signicant reduction of IL-6 level in endometrium (12%)
and prostate (98.8%) cancer cell lines while a signicant increase was observed in TNF-α level in endometrium
(385%) and prostate (89.8%) cancer cell lines as compared to control. No alteration of PSA level was observed
in bioeld treated endometrium and prostate cell line. Similarly, no alterations were evident in IL-2R and FPSA
levels in endometrium and prostate cell lines after bioeld treatment as compared to control. In conclusion, results
suggest that bioeld treatment has shown signicant alterations in the level of cytokines (IL-6 and TNF-α) in both
endometrium and prostate cancer cell lines.
In Vitro
Evaluation of Biofield Treatment on Cancer Biomarkers Involved
in Endometrial and Prostate Cancer Cell Lines
Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Mayank Gangwar2 and Snehasis Jana2*
1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA
2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India
Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 254
control and treated sample to observe the impact of bioeld treatment
on concentration of biomarkers (IL-2R, IL-6, TNF-α, PSA, and FPSA)
[28]. ELISA principle started with capturing antibody, specic for a
protein of interest, coated onto the wells of microplates. Control and
bioeld treated cell lysate samples were diluted and pipetted into the 96
micro wells titer plate. Buer was added into each well and mixed gently
for 30 seconds. e plate was covered and incubated for 60 minutes
at room temperature as per manufacturer’s instruction. Removed the
incubation mixture from all wells followed by washing with distilled
water for ve times. During this incubation, protein of interest i.e.,
antigen (TNF-α, IL-6, PSA, FPSA, and IL-2R) attached to the antibody.
A secondary antibody was added to the wells aer washing with buer,
which bound to immobilized protein captured during rst incubation.
Excess antibody was removed by washing with the help of wash buer.
Aer that, enzyme conjugate was added into each well, mixed gently for
10 seconds followed by incubation for 60 minutes at room temperature.
A specic substrate solution was added into the well which was
converted by the enzyme to form a detectable color. e intensity of
the color produced was read spectrophotometrically which was directly
proportional to the concentration of protein of interest present in the
cell line samples. Control and treated sample values were recorded
in picogram per milliliter (IL-6 and TNF-α: pg/ml), nano gram per
milliliter (PSA and FPSA: ng/ml), and unit per milliliter (IL-2R-U/ml) [29].
Results and Discussion
Since prostate and endometrium cancers are most critical
medical problems aecting major population, physician suggests
chemoprevention strategies to reduce the incidence and mortality rate.
However, there are scientic evidence and large number of in vitro
studies already reported for bioeld treatment and its eect in cancer
cell lines [19,20]. e present study aimed to examine Mr. Trivedi’s
bioeld treatment on cancer biomarkers associated with endometrium
and prostate cancer. Previous studies indicated that there were an
inhibitory eect of bioeld energy on metastasis and immune responses
on cancer cell models [30-33].
Results of control and treatment group, at day 10 are summarized
in Table 1. In case of endometrium cell line, 12% reduction in IL-6
concentration was found in bioeld treated group as compared to
control. Aer bioeld treatment, decreased level of IL-6 (3.52 pg/
ml) was reported as compared to control (4.0 pg/ml). A highly
signicant increase in TNF-α (385%) level was estimated in bioeld
treated endometrium cell line (20.5 pg/ml) as compared to control
(4.23 pg/ml). A high increase in TNF-α level might be its association
and binding with several other cells such as endothelial cells, which
results in its high expression aer bioeld treatment as compared as
control [34]. However, level of PSA, FPSA, and IL-2R did not show
any alteration aer bioeld treatment as compared to control. In
endometrial adenocarcinoma, cytokines promote tumor development
by angiogenesis and mediating cell invasion. TNF-α, pro-inammatory
cytokine biomarker exists as both soluble and membrane-integrated
proteins activates signaling pathways, and is expressed on surface
epithelium in cancer and endometrial adenocarcinoma. It promotes
angiogenesis by the activation of NF-κB, a protein complex [35]. Apart
from TNF-α, IL-6 is another pro-inammatory cytokines whose level is
upregulated in endometrial adenocarcinoma, which is associated with
a poor prognosis [36]. Cancer research now proofed that dysregulation
in immune system contributes to metastasis and lead to cell death.
However, cancer progression is indicative by chronic inammation
which enhances angiogenesis and tissue metabolism results in cytokines
release. erefore, a signicant alteration of important inammatory
(NCCAM), now dened bioeld therapies in the subcategory of energy
therapies as one of the ve complementary medicine domains. Bioeld
is the name given to the electromagnetic eld that permeates and
surrounds living organisms. It is the scientically preferred term for
the biologically produced electromagnetic and subtle energy eld that
provides regulatory and communic ation funct ions within the organism.
Recent studies on cancer research suggest that instead of using other
complementary and alternate medicine, cancer patients received
highest benet with energy medicine [15]. e concept of human
bioenergy has its origin thousands of year back, till date many recent
bioeld therapies are in practice for their possible therapeutic potentials
such as enhanced personal well-being in cancer patient [16], improved
functional ability of arthritis patient [17], decreased pain and anxiety
[18]. However, many other signicant inhibitory eects on the cancer
cell model have been published which suggest that bioeld treatment
could be a new and eective treatment approach for cancer [19,20]. Mr.
Mahendra Trivedi’s unique bioeld treatment (e Trivedi’s eect®) has
signicantly studied in dierent elds. In agr iculture, change on growth,
characteristics, and yield of plants were reported aer bioeld treatment
[21,22]. It has also signicantly altered the crystalline and powder
characteristics of metals [23,24] and transformation of bioeld energy
has considerably altered the genotype and phenotypes of pathogenic
microbes against antimicrobials [25-27]. is experiment was designed
to evaluate the impact of bioeld treatment on cancer biomarkers in
endometrium and prostate cancer cell lines. Concentration of cytokines
(i.e. TNF-α and IL-6), interleukin-2 receptor (IL-2R), prostate specic
antigen (PSA), and free prostate specic antigen (FPSA) were estimated
by using ELISA assay on day 10 aer bioeld treatment as compared to
control.
Materials and Methods
Experimental design
Endometrium and prostate cancer cell lines, as stock stored cultures
were procured for the experiment from department of laboratory
medicine, P.D. Hinduja National Hospital & Medical Research Centre,
Mumbai. Enzyme-Linked Immuno-Sorbant Assay (ELISA) method
was used for estimation of cytokines levels. Important cancer diagnostic
biomarkers such as IL-6, TNF-α, PSA (third generation), FPSA, and IL-
2R of control and treated samples were estimated using ELISA kit as per
manufacturer’s instruction.
Bioeld treatment modalities
Endometrium and prostate cancer cell lines were taken for bioeld
treatment. e rst sets of both cancer cell line were considered as
control. No treatment was given to this set. e second sets of both
cancer cell line were handed over to Mr. Trivedi for bioeld treatment
under laboratory condition. Mr. Trivedi provided the bioeld
treatment through his energy transmission process to second sets of
samples without touching the samples. Aer treatment, both treated
samples were handed over in the same condition within an hour’s
time and stored at standard conditions for cytokines analysis aer
10 days. Both the control and treated samples were analyzed aer 10
days for dierence in cancer biomarker as per the standard protocols.
An optimum precautionary measure were taken in estimation of IL-
6, TNF-α, PSA (third generation), FPSA, and IL-2R throughout the
experiment. e dierences in tested cancer biomarkers level before
and aer the treatment were noted.
Measurement of diagnostic cancer biomarkers
ELISA was performed as per the manufacturer’s instructions in
Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 255
biomarkers (IL-6 and TNF-α) in endometrium cancer cell line suggests
that bioeld treatment may aect metastasis, which results in alteration
of pleiotropic and multifunctional cytokine levels. is nding is the
rst evidence that bioeld therapy may alter the immune function in
endometrium cancer.
Bioeld treatment on prostate cancer cell line showed a signicant
reduction of as high as 98.8% in IL-6 level. Aer treatment, IL-6
concentrations showed less than 2 pg/ml as compared to control (165
pg/ml) in prostate cancer cells. Besides this, TNF-α level showed
an increase of 89.8% in bioeld treated group (i.e.7.59 pg/ml) as
compared to control (less than 4.0 pg/ml). Slight increase in level of
PSA was observed in bioeld treated prostate cell line as compared to
control (Table 1). However, level of FPSA and IL-2R did not show any
alteration aer bioeld treatment as compared to control. It suggests
that the bioeld treatment has signicant impact only on cytokines
level and not on receptors or antigens involved in metastasis. Above
cytokines have the major involvement in prostate cancer development
and elevated levels have been reported in prostate hyperplasia patients
[37]. IL-6 and TNF-α levels have strong correlation in serum with
hormone refractory prostate cancer as compared to normal cell [38].
Present study reported a signicant reduction in IL-6 concentration
and an increase in TNF-α level in prostate and endometrium cancer
cell lines aer bioeld treatment (Figure 1). e result of the present
study supports the already published literature with signicant bioeld
eect on cancer cell lines. is study results are in agreement with the
reported data [31-33] and verify the inuence of bioeld treatment
on in vitro growth of prostate and endometrium cancer cell line with
respect to cancer biomarkers level in control and treated samples.
Current cancer therapies have certain limitations, which include
serious side eects such as enormous toxicity, altered immune system,
and high treatment cost etc. Many ancient bioeld therapies for cancer
Figure 1: Percent change in cytokine level measured using ELISA assay on day 10 after bioeld treatment in (a) endometrium cell line (b) prostate cell line.
S. No. Biomarker Endometrium cell line Prostate cell line
Control Treated % change Control Treated % change
1IL-2R (U/ml) <50.0 <50.0 NC <50.0 <50.0 NC
2 IL-6 (pg/ml) 4.0 3.52 -12 165 < 2.0 -98.8
3TNF-α (pg/ml) 4.23 20.5 385 < 4.0 7.59 89.8
4 PSA (ng/ml) < 0.003 < 0.003 NC < 0.003 0.003 NC
5 FPSA (ng/ml) < 0.05 < 0.05 NC < 0.05 < 0.05 NC
NC: No change; pg/ml: picogram per milliliter; ng/ml: Nano gram per milliliter; U/ml: Unit per milliliter; IL-2R: Interleukin-2 receptor; IL-6: Interleukin-6; TNF-α: Tumor necrosis
factor alpha; PSA: Prostate specic antigen; FPSA: Free prostate specic antigen.
Table 1: Effect of bioeld treatment on cancer biomarker concentration in endometrium and prostate cancer cell lines.
Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 256
are practiced around the world, among then Qigong therapy for cancer
is very popular and scientically studied [39,40]. erefore, Mr. Trivedi’s
bioeld treatment could be an eective and alternate approach for
cancer with no side eects. Research on bioeld therapies and its impact
on cancer growth have been continuously studied by researchers, which
might works as alternate and complementary medicine to prolong the
life of cancerous patients along with quality life.
Conclusion
Study results conclude that bioeld treatment has signicantly
altered the level of cytokines (IL-6 and TNF-α) in endometrium and
prostate cancer cell lines as compared to control. Mr. Trivedi’s bioeld
treatment showed a signicant reduction in IL-6 level in prostate
cancer cells (98.8%) and endometrium cancer cell (12%) as compared
to control. ere was also a signicant increase of TNF-α concentration
in both endometrium and prostate cancer cell lines. ese data suggest
that bioeld treatment has signicant impact in altering the cancer
biomarkers especially two cytokine levels in both cancer cell lines.
Acknowledgement
Authors gratefully acknowledged to support of Trivedi science, Trivedi
testimonials and Trivedi master wellness in this research work.
Conict of interest
The authors declare that they have no competing interests.
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Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
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Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In
Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257.
doi:10.4172/1948-5956.1000358
... However, the National Center for Complementary and Alternative Medicine (NCCAM), well-defined Biofield Therapies in the subcategory of Energy Therapies [14]. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported to have created significant changes in the physicochemical properties of metals, chemicals, ceramics and polymers [15][16][17], improved agricultural crop yield, productivity, and quality [18,19],transformed antimicrobial characteristics [20][21][22], biotechnology [23,24], improved bioavailability [25][26][27], skin health [28,29], nutraceuticals [30,31], cancer research [32,33], bone health [34][35][36], human health and wellness. From outstanding benefits of Biofield Energy Treatment, the present study aimed to evaluate the impact of the Biofield Energy Treated (The Trivedi Effect ® ) DMEM on ED using the standard in vitro assay in Human Endothelial Hybrid Cell Line (EA. ...
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Erectile dysfunction is a common disorder found in men, which occurs due to multiple factors such as psychogenic, hormonal imbalance, and neurovascular disturbances. The present investigation was undertaken to examine the effect of a Consciousness Energy Healing based DMEM medium on the Human Endothelial Hybrid Cell Line (EA. hy926) to evaluate the level of cyclic guanosine monophosphate (cGMP). The test item (DMEM medium) was divided into three parts, first part received a one-time Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Alice Branton and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times the Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. The level of cGMP for the inhibition of PDE-5 enzyme was assessed using cGMP ELISA assay kit (colorimetric). Sildenafil citrate, used as positive control, which showed a significant increase of cGMP levels in Ea. hy926 cells. The one-time Biofield Energy Healing Treated DMEM (BT-I) showed 29.92% alteration, while the two-times Biofield Energy Healing Treated DMEM (BT-II) showed a significantly (p≤0.001) increased level of intracellular cGMP by 307.09% in Ea. hy926 cells compared to the untreated DMEM group. Overall, experimental data suggested that the two-times Biofield Treated DMEM showed a significantly improved level of cGMP compared with the one-time Biofield Treated DMEM group. Therefore, data indicated that the Biofield Energy Healing Treatment can be used to treat erectile dysfunction along with other sexual disorders such as orgasmic disorders, female sexual arousal disorder, fetishistic disorder, frotteuristic disorder, hypoactive sexual desire disorder, sex addiction, sexual masochism and sadism, vaginismus, voyeuristic disorder, premature or delayed ejaculation.
... Biofield based Energy Healing Therapies accepted worldwide for the use against various human disease conditions and has been accepted as a Complementary and Alternative Medicine (CAM) health care approach by the National Center of Complementary and Integrative Health (NCCIH) along with Tai Chi, Reiki, hypnotherapy, Ayurvedic medicine, yoga, Qi Gong, aromatherapy, meditation, traditional Chinese herbs and medicines, homeopathy, chiropractic/osteopathic manipulation, acupuncture, acupressure, naturopathy, cranial sacral therapy, movement therapy, etc [31][32][33]. Biofield Energy Treatment has also been reported in many peer-reviewed scientific journals with significant outcomes in microbiology, biotechnology, medical research, and agricultural science [34][35][36][37][38][39][40][41][42]. The physicochemical properties of a drug have a vital role in the drug during processing, formulation, packaging, and storage [43,44]. ...
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Zinc chloride is commonly used as a source of zinc for the treatment of many diseases and overall maintenance of good health. The impacts of the Trivedi Effect® on the physicochemical, thermal, and behavioral properties of zinc chloride were evaluated using sophisticated analytical techniques. Zinc chloride was divided into control and treated parts. Only, the treated part received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The powder XRD relative peak intensities and crystallite sizes of the treated zinc chloride were significantly altered ranging from -58.73 to 141.43 and -74.03 to 228.56%, respectively compared with the control sample. The average crystallite size of the treated zinc chloride was significantly increased by 4.34% compared with the control sample. The particle sizes of the treated zinc chloride sample were significantly altered by 11.04% (d10), -11.85% (d50), -20.35% (d90), and -21.88% {D (4, 3)} compared with the control sample. Thus, the surface area of the treated zinc chloride (10400 m2/g) was significantly increased by 112.24% compared with the control sample (4900 m2/g). The latent heat of fusion and decomposition of the treated sample were significantly increased by 96.18% and 134.66%, respectively compared with the control sample. The current study predicted that the Trivedi Effect®-Consciousness Energy Healing Treatment might produce a new polymorphic form of zinc chloride with larger crystallite size, smaller particle size, and increased surface area, which would improve the solubility, absorption, bioavailability, and thermal stability compared with the control sample. Therefore, the Consciousness Energy Healing Treated zinc chloride would be very useful to design more efficacious nutraceutical or pharmaceutical formulations for the treatment of immunological disorders, inflammatory diseases, aging, stress, cancer, parakeratosis, dysosmia, hypogonadism, Wilson’s disease, anorexia, etc.
... This energy can be harnessed and transmitted by the experts into living and non-living things via the process of Biofield Energy Healing. Biofield Energy Treatment (The Trivedi Effect ® ) has been published in numerous peer-reviewed science journals with significant outcomes in many scientific fields such T as cancer research [9,10], microbiology [11][12][13][14], biotechnology [15,16], pharmaceutical science [17][18][19][20], agricultural science [21][22][23][24], materials science [25][26][27][28], nutraceuticals [29,30], skin health, human health and wellness. ...
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Hair follicle growth and maturation are potentially useful for the treatment of skin injuries and diseases. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was denoted as the untreated test item without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Dahryn Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3.
... This energy can be harnessed and transmitted by the experts into living and non-living things via the process of Biofield Energy Healing. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported with a significant revolution in the field of cancer research [11,12], materials science [13][14][15], microbiology [16][17][18], agriculture [19,20], nutraceuticals [21,22], and biotechnology [23,24]. Besides, The Trivedi Effect ® also significantly improved bioavailability of various low bioavailable compounds [25][26][27], an improved overall skin health [28,29], bone health [30][31][32], human health and wellness. ...
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The study objective was to evaluate the cardioprotective activity of Biofield Energized test item (DMEM) in rat cardiomyocytes (H9c2) cells. The test item (DMEM medium) was divided into three parts, first part received one-time Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the one-time Biofield Energy Treated (BT-I) DMEM, while second part received the two-times Biofield Energy Treatment and is denoted as BT-II DMEM. The third part did not receive any treatment and defined as the untreated DMEM group. MTT cell viability assay of the test sample showed more than 95% viable cells in the BT-I and BT-II groups, respectively suggested a nontoxic nature of the test substance. The BT-II group showed 16.28% restoration of cytoprotective function compared to the t-BHP induced group. The level of lactate dehydrogenase (LDH) was significantly inhibited by 48.36% and 47.89% in the BT-I and BT-II groups, respectively compared to t-BHP induced group. Level of creatine kinasemyocardial band (CK-MB) was significantly suppressed by 59.5% in the BT-I and BT-II groups compared to the t-BHP induced group. Reactive oxygen species (ROS) level was significantly suppressed by 120.08% and 116.20% in the BT-I and BT-II groups, respectively. Besides, BT-I and BT-II groups showed 33% and 48.5% inhibition of apoptotic cells, respectively compared to the t-BHP induced group. Altogether, result suggested that Biofield Treated test items significantly improved different cardiac parameters. Thus, Consciousness Energy Healing (The Trivedi Effect®) Treatment could be utilized as a cardio-protectant against several cardiac disorders such as heart failure, coronary artery disease, heart attack, congenital heart disease, cardiomyopathy, arrhythmias, etc.
... The Biofield Energy Healing therapy has been acknowledged as a Complementary and Alternative Medicine (CAM) health care approach for the improvement the quality of life by the National Center of Complementary and Integrative Health with other therapies, medicines and practices such as Qi Gong, yoga, chiropractic/osteopathic manipulation, cranial sacral therapy, Tai Chi, meditation, healing touch, homeopathy, acupuncture, acupressure, hypnotherapy, naturopathy, movement therapy, Ayurvedic medicine, aromatherapy, Reiki, etc. [15,16]. Biofield Energy Healing Treatment is drawing importance in different scientific fields include material science, pharmaceuticals, nutraceuticals, organic compounds, microbiology, agricultural, biotechnology, genetics medical [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. The solid state properties like physicochemical and thermal properties of a pharmaceutical compound play an important role in drug performance, i.e. bioavailability, therapeutic efficacy, and toxicity [34,35]. ...
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... Biofield Energy Healing as a CAM showed significant results in biological studies [10]. Biofield Energy Healing Treatment has been reported with considerable revolution in the physicochemical properties of metals, chemicals, ceramics and polymers [12][13][14], improved agricultural crop yield, productivity, and quality [15,16], transformed antimicrobial characteristics [17][18][19], biotechnology [20,21], improved bioavailability [22][23][24], improved skin health [25,26], nutraceuticals [27,28], cancer research [29,30], bone health [31][32][33], human health and wellness. From Biofield Energy Treatment outcome, authors in this study evaluate the impact of the Biofield Energy Treatment (The Trivedi Effect ® ) on DMEM as a test sample for estrogenic potential with respect to ALP using the standard in vitro assay in Ishikawa cells. ...
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Alkaline phosphatase (ALP) plays a vital role in the endometrium development, maturation, endocrine function, and osteoblastic differentiation. The present investigation explained the role of Consciousness Energy Healing based test item (DMEM medium) on ALP in Ishikawa cells. After the division of the test item into two parts; first, part received Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Treated DMEM, while the second part did not receive any treatment, and denoted as the untreated DMEM. MTT data showed 142.56% viable cells in the Biofield Treated test item, which indicated that the test item was found as safe and non-toxic. Besides, the estrogenic potential concerning ALP level was significantly (p≤0.001) increased by 65.18% in the Biofield Treated DMEM group than untreated DMEM. Thus, the experimental data suggested that the Biofield Energy Treated DMEM would play a significant role for the growth of Ishikawa cells along with an improved ALP level, which play a vital role in the promotion and maintenance of estrogen level and can be used against various estrogenic disorders viz. endometritis, hypophosphatasia, osteoporosis, infertility, severe anemia, malnutrition, fetal defects, early pregnancy loss, and preterm birth.
... This energy can be harnessed and transmitted by the experts into living and non-living things via the process of Biofield Energy Healing. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported with a significant revolution in the field of materials science [12][13][14] , agriculture [15,16] , microbiology [17][18][19] , biotechnology [20,21] , nutraceuticals [22,23] , cancer research [24,25] . Apart from this, The Trivedi Effect ® also tremendously improved bioavailability of various low bioavailable compounds [26][27][28] , an improved overall skin health [29,30] , bone health [31][32][33] , human health and wellness. ...
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Liver is the main metabolic organ in human. Most of the chemicals are primarily metabolized in the liver followed by excretion of the metabolites from the body. Therefore, there is a high chance of liver damage and most of the peoples are suffering many diseases due to their poor metabolic power of liver. In this context, the present study was performed to find out the potential role of the Biofield Energy (The Trivedi Effect®) Treated test item (DMEM) in HepG2 cell line. The test item was divided into two parts. One part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and the other part defined as the untreated DMEM, where no Biofield Treatment was given. Cell viability of the test sample using MTT assay showed 152.8 % viable cells in the Biofield Energy Treated DMEM group, suggested that the test item was nontoxic and safe in nature. The Biofield Energy Treated DMEM showed 12.2 % protection of cells against oxidative stress induced by t-BHP. The level of IL-8 was significantly reduced by 13.71 % in the Biofield Energy Treated DMEM group compared to the TNF-α stimulated control group. Moreover, the level of ALT was significantly (p ≤ 0.001) reduced by 64.16 % in the Biofield Energy Treated DMEM group compared to the t-BHP group. Cholesterol level was significantly reduced by 34.94 % Biofield Energy Treated DMEM group compared to the untreated DMEM group. Besides, the Biofield Energy Treated DMEM group showed 23.19 % increased the level of albumin compared to the untreated DMEM group. Altogether, results suggested that Biofield Treatment significantly improved liver health and its function. Thus, Consciousness Energy Healing (The Trivedi Effect®) Treatment could be utilized as a hepatoprotectant against several hepatic disorders such as Gilbert’s disease, cirrhosis, steatosis, alcohol abuse, hemochromatosis, Budd-Chiari syndrome, Wilson’s disease, cholangiocarcinoma, etc.
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The present in vitro investigation was aimed to explore the effect of Consciousness Energy Healing Treated DMEM medium for oxidative potential using oxidative biomarkers viz. Superoxide Dismutase (SOD) enzyme activity and protection against oxidative stress measured in A549 cells. The test item, DMEM was divided into two parts. First part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Energy Treated DMEM group, while the second part did not receive any sort of Treatment and defined as the Untreated DMEM (control) group. The cell viability of the test sample using MTT assay showed an increase cell viability by 136.4% in the Biofield Energy Treated DMEM group, suggesting a safe and nontoxic nature of the test items. Protection against oxidative stress was significantly increased by 23.80% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Besides, SOD enzyme activity was significantly increased by 40.40% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Thus, the overall data suggest that the Biofield Energy Healing Treatment showed a significant improvement of SOD enzyme level along with an improved protection against oxidative damage, which can be used in various human lungs disorders such as asthma, Chronic Obstructive Pulmonary Disease (COPD), lung malignancies and parenchymal lung diseases like idiopathic pulmonary fibrosis and lung granulomatous diseases.
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The aim of the present research investigation was to monitor the effect of Consciousness Energy Healing based DMEM medium in a synovial sarcoma cell line (SW982) for evaluation of bone health (anti-rheumatoid arthritis) potential using inflammatory parameter interleukin-8 (IL-8). The test item (DMEM) was divided into two parts. One part was represented as the untreated test item without any Biofield Energy Treatment, while the other portion was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi. MTT assay was used for testing the cell viability and the results showed that the cell viability of SW982 cells was more than 100% with a safe and non-toxic profile. Further, the test item showed a significant (p≤0.001) inhibition of IL-8 secretion by 70.05% under the stimulation of IL-1β in SW982 cells as compared to the untreated DMEM group. Overall, the experimental data revealed the significant potential of the test item against rheumatoid arthritis to reduce inflammation in joints. Thus, it is concluded that Consciousness Energy Healing Treatment significantly inhibits the pro-inflammatory cytokine (IL-8), which showed a potential to act as an anti-rheumatic action in arthritis and other bone inflammatory disorders such as osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, osteoma, aging, bone loss, and fractures.
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Hair loss directly impacts people’s social interactions and also affects psychological well-being. Hair fall has been a rising trend nowadays due to dynamic lifestyle and pollution. In this context,the present study was performed to identify the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (mixture of herbal ex-tracts of Phyllantus emblica and Eclipta alba in 1:1 ratio) on the telogen skin of mice for the assessment of haircell growth and development in vivo. The test item was divided into two parts. One part was denoted as the untreated test item without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Dahryn Trivedi. The study parameters like anagen induction and visual melanogenesis were performed using skin biopsy technique. The experimental results of the untreated and Biofield Energy Treated test item groups showed hair growth with 50% and 60%, respectively on dorsal clipped of skin after topical application. Besides, the Biofield Energy Treated test item group exhibited 60% melanogenesis after biopsy analysis in mice skin at the end of the experiment. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased hair growth and melanogenesis. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment could be useful as a hair growth promoter for various treatments of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer, etc.
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In earlier papers the effect of Mr. Trivedi's thought intervention through biofield in his physical presence on the atomic, crystalline and particle characteristics of first series of transition metal powders, group four metals and carbon allotropes are discussed. In the present paper we demonstrate this unusual effect on sieve size distribution, apparent density and flow of several metal powders under PM plant conditions.
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Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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This study tested the Null Hypothesis for the effect of BioField Energy applied to two separate crops under typical growing conditions, namely ginseng and organic blueberry in commercial plantings in Wisconsin and California, respectively. Following treatment to replicated plots in standard experimental design, data were collected at harvest for yield quantity and quality. Ginseng plants treated both pre-harvest and a combination of pre- and post-harvest showed market grade increases of 33.3% and 40.0%, respectively. Point of sale gross return for this crop is dependent upon tuber quality, and from these data the economics of these treatments were calculated. Based on stand adjusted yields and quality values, a combination of pre- and post-harvest treatment increased gross income by 57.4%. The second crop showed similar trends in positive responses. In the two blueberry varieties studied, Emerald treated plants showed 96% statistical increase in yield, while Jewel showed 31% increase. At the time of treatment, each variety was in a different stage of flowering. The Emerald variety was in the flowering stage, and Jewel was predominately in the fruiting stage. Both treated cultivars however demons-trated increased yield quantity and quality. The specific mechanisms that lead to these pre-liminary results need further investigation.
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Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC –51299], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists Results: The results indicated that Mr.Trivedi’s energy has changed 9 of 27 biochemical characteristics of Enterococcus faecalis along with significant changes in susceptibility pattern in 5 of 31 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy/treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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Purpose While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists. Results The results indicated that Mr.Trivedi’s energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
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Breast cancer is one of the leading causes of cancer death among women of all races. Pain is a common symptom associated with cancer; 75–90% of cancer patients experience pain during their illness and up to 50% of that pain is undertreated. Unrelieved pain leads to increased levels of the stress hormone cortisol. The purpose of this study was to examine the impact of bioenergy on fecal cortisol levels for mice injected with murine mammary carcinoma 4T1in two separate pilot studies. Using a multiple experimental group design, six to eight week old female BALB/c mice were injected with tumor and randomly assigned, in groups of 10, to daily treatment, every other day treatment, and no treatment groups. Five days after tumor cell injection, bioenergy interventions were begun for a period of ten consecutive days. Fecal samples were collected for each study and ELISA analysis was conducted at the end of both studies. For both studies, cortisol levels were decreased in the every other day treatment groups but remained high in the no treatment groups. Future studies utilizing bioenergy therapies on cortisol levels in a murine breast cancer model can begin to describe pain outcomes and therapeutic dose.
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To date there has been no consensus on the exact oncological importance of systematic lymphadenectomy in early stage endometrial cancer. In this scenario, the balance between potential therapeutic benefit and procedure-related morbidity plays a central role in the indication for the procedure since the likelihood of detecting a metastatic node is relatively low. Compared with laparotomy, laparoscopic lymphadenectomy has several clearly demonstrated advantages and is an important tool to reduce morbidity. Additionally, many authors have developed less invasive methods to selectively identify patients who are at increased risk of lymph node involvement. This paper aims to review the current literature evidence and guidelines regarding the role of lymphadenectomy in patients with early stage endometrial cancer. Alternatives such as lymphatic sampling or sentinel lymph node biopsy are possible solutions but must be further investigated through more comprehensive studies.
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Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The combination of biofield treatment along with administration of chemical additives demonstrated the best results with statistically increased yields and higher pest resistance in both test cropping systems. The specific mechanisms that lead to these preliminary results have yet to be determined.
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To date there has been no consensus on the exact oncological importance of systematic lymphadenectomy in early stage endometrial cancer. In this scenario, the balance between potential therapeutic benefit and procedure-related morbidity plays a central role in the indication for the procedure since the likelihood of detecting a metastatic node is relatively low. Compared with laparotomy, laparoscopic lymphadenectomy has several clearly demonstrated advantages and is an important tool to reduce morbidity. Additionally, many authors have developed less invasive methods to selectively identify patients who are at increased risk of lymph node involvement. This paper aims to review the current literature evidence and guidelines regarding the role of lymphadenectomy in patients with early stage endometrial cancer. Alternatives such as lymphatic sampling or sentinel lymph node biopsy are possible solutions but must be further investigated through more comprehensive studies.