In Vitro Evaluation of Biofield Treatment on Cancer Biomarkers Involved in Endometrial and Prostate Cancer Cell Lines

Abstract

Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. This study aimed to investigate the effect of biofield treatment on cancer biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Trivedi’s biofield treatment, referred as treated. Control and treated samples were studied for the determination of cancer biomarkers such as multifunctional cytokines viz. interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), interleukin-2 receptor (IL-2R), prostate specific antigen (PSA), and free prostate specific antigen (FPSA) concentrations using ELISA assay on day 10. Experimental results showed a significant reduction of IL-6 level in endometrium (12%) and prostate (98.8%) cancer cell lines while a significant increase was observed in TNF-α level in endometrium (385%) and prostate (89.8%) cancer cell lines as compared to control. No alteration of PSA level was observed in biofield treated endometrium and prostate cell line. Similarly, no alterations were evident in IL-2R and FPSA levels in endometrium and prostate cell lines after biofield treatment as compared to control. In conclusion, results suggest that biofield treatment has shown significant alterations in the level of cytokines (IL-6 and TNF-α) in both endometrium and prostate cancer cell lines.

Full text

Open Access
Research Article
Cancer
Science & Therapy
J
o
u
r
n
a
l
o
f
C
a
n
c
e
r
S
c
i
e
n
c
e
&
T
h
e
r
a
p
y
ISSN: 1948-5956
Trivedi et al., J Cancer Sci Ther 2015, 7:8
http://dx.doi.org/10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 253
Keywords: Bioeld treatment; Cancer biomarker; ELISA; TNF-α;
IL-6; Prostate cancer; Endometrium cancer
Introduction
Cancer has the potential to invade or spread to other parts of the
body which involves abnormal cell growth. Endometrium cancer
(EC) is the most common type of uterine cancer which starts in the
endometrium. EC cases are increasing worldwide and even higher in
developing countries [1]. However, physicians have developed new
diagnostic techniques and treatments, which results in decreased
reported cases of cancer in developing countries [2]. Endometrioid
adenocarcinoma is the most common type of EC with clear cell
histologic appearance made up of cells in glands that look much like
the normal uterine lining (endometrium) [3]. e survival rate in an
early and advanced stage of diagnosed EC is less than ve years [4],
and chances of about 50% recurrence aer postoperative therapy is
still high [5]. Most common cancer in male, especially in the western
world causing death is prostate cancer (PC). In the United States,
approximately 238,590 new cases of PC with a death of about 29,720
cases [6]. Progression of PC is through a multi-step development such
as involvement of growth factor, cytokines, and hormones [7].
Role of immune cells estimation in microenvironment of tumor
has been well established. On the primary hormonal basis, these
immune cells in tumor respond to produce a wide range of cytokines,
chemokines, and growth factors. Interleukins (IL-1, IL-6, and IL-
8), tumor necrosis factor-α (TNF-α), vascular endothelial growth
factor (VEGF), granulocyte-macrophage colony stimulating factor
(GM-CSF), and colony stimulating factor-1 (CSF-1) are the common
inammatory markers used in diagnosis [8]. IL-6 and TNF-α, are
considered as the major cancer biomarkers of a network involved in
EC and PC development with multiple biological characteristics [9,10].
TNF-α, is a multifunctional cytokine which plays a major role in cell
death and survival, as well as in inammation and immunity. TNF-α
increases susceptibility to heat-induced cell death (apoptosis) in a time-
dependent manner, an important protective cellular activity. IL-6 is a
pleiotropic cytokine, which exerts inammatory and immune response
on various cell types including prostate carcinomas. Serum IL-6 is the
surrogate marker in the clinical stage of PC. High concentrations of
serum IL-6 are associated with an increased risk of death in patients with
cancer. Expression of IL-2R, PSA and FPSA are the robust biomarkers
in predicting a diagnosis assay for prostate cancer. Normally, IL-2R and
IL-6 concentrations were signicantly higher in prostate cancer tissue
than in normal tissue.
In the last 70 years, cancer treatment strategies have rapidly
increased. Chemotherapy always associated with attached side eects
such as hair loss, nausea, vomiting, cognitive dysfunction, change in
sexual function and adverse impact on patient’s quality of life [11].
Surgery is still the main treatment strategy for early-stage diagnosis of
cancer, but for advanced and reoccurrence cases multimodality therapies
like radiations and chemotherapy being frequently used [12,13].
Increased use of alternative integrative medicine approaches have been
recently introduced and used to promote wellness and minimize side
eects from cancer. In the year 2007, 40% U.S. population has used
natural products as a complementary and alternate medicine (CAM)
[14]. National Center for Complementary and Alternative Medicine
*Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory
Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd.,
Bhopal- 462026, Madhya Pradesh, India, Tel: +91-755-6660006; E-mail:
publication@trivedisrl.com
Received June 24, 2015; Accepted July 30, 2015; Published August 03, 2015
Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro
Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in Endometrial
and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-
5956.1000358
Copyright: © 2015 Trivedi MK, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited.
Abstract
Increasing cancer rates particularly in the developed world are associated with related lifestyle and
environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches
in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening
problems to promote human wellness. This study aimed to investigate the effect of bioeld treatment on cancer
biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in
two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Trivedi’s bioeld
treatment, referred as treated. Control and treated samples were studied for the determination of cancer biomarkers
such as multifunctional cytokines viz. interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), interleukin-2
receptor (IL-2R), prostate specic antigen (PSA), and free prostate specic antigen (FPSA) concentrations using
ELISA assay on day 10. Experimental results showed a signicant reduction of IL-6 level in endometrium (12%)
and prostate (98.8%) cancer cell lines while a signicant increase was observed in TNF-α level in endometrium
(385%) and prostate (89.8%) cancer cell lines as compared to control. No alteration of PSA level was observed
in bioeld treated endometrium and prostate cell line. Similarly, no alterations were evident in IL-2R and FPSA
levels in endometrium and prostate cell lines after bioeld treatment as compared to control. In conclusion, results
suggest that bioeld treatment has shown signicant alterations in the level of cytokines (IL-6 and TNF-α) in both
endometrium and prostate cancer cell lines.
In Vitro
Evaluation of Biofield Treatment on Cancer Biomarkers Involved
in Endometrial and Prostate Cancer Cell Lines
Mahendra Kumar Trivedi1, Shrikant Patil1, Harish Shettigar1, Mayank Gangwar2 and Snehasis Jana2*
1Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA
2Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-462026, Madhya Pradesh, India

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 254
control and treated sample to observe the impact of bioeld treatment
on concentration of biomarkers (IL-2R, IL-6, TNF-α, PSA, and FPSA)
[28]. ELISA principle started with capturing antibody, specic for a
protein of interest, coated onto the wells of microplates. Control and
bioeld treated cell lysate samples were diluted and pipetted into the 96
micro wells titer plate. Buer was added into each well and mixed gently
for 30 seconds. e plate was covered and incubated for 60 minutes
at room temperature as per manufacturer’s instruction. Removed the
incubation mixture from all wells followed by washing with distilled
water for ve times. During this incubation, protein of interest i.e.,
antigen (TNF-α, IL-6, PSA, FPSA, and IL-2R) attached to the antibody.
A secondary antibody was added to the wells aer washing with buer,
which bound to immobilized protein captured during rst incubation.
Excess antibody was removed by washing with the help of wash buer.
Aer that, enzyme conjugate was added into each well, mixed gently for
10 seconds followed by incubation for 60 minutes at room temperature.
A specic substrate solution was added into the well which was
converted by the enzyme to form a detectable color. e intensity of
the color produced was read spectrophotometrically which was directly
proportional to the concentration of protein of interest present in the
cell line samples. Control and treated sample values were recorded
in picogram per milliliter (IL-6 and TNF-α: pg/ml), nano gram per
milliliter (PSA and FPSA: ng/ml), and unit per milliliter (IL-2R-U/ml) [29].
Results and Discussion
Since prostate and endometrium cancers are most critical
medical problems aecting major population, physician suggests
chemoprevention strategies to reduce the incidence and mortality rate.
However, there are scientic evidence and large number of in vitro
studies already reported for bioeld treatment and its eect in cancer
cell lines [19,20]. e present study aimed to examine Mr. Trivedi’s
bioeld treatment on cancer biomarkers associated with endometrium
and prostate cancer. Previous studies indicated that there were an
inhibitory eect of bioeld energy on metastasis and immune responses
on cancer cell models [30-33].
Results of control and treatment group, at day 10 are summarized
in Table 1. In case of endometrium cell line, 12% reduction in IL-6
concentration was found in bioeld treated group as compared to
control. Aer bioeld treatment, decreased level of IL-6 (3.52 pg/
ml) was reported as compared to control (4.0 pg/ml). A highly
signicant increase in TNF-α (385%) level was estimated in bioeld
treated endometrium cell line (20.5 pg/ml) as compared to control
(4.23 pg/ml). A high increase in TNF-α level might be its association
and binding with several other cells such as endothelial cells, which
results in its high expression aer bioeld treatment as compared as
control [34]. However, level of PSA, FPSA, and IL-2R did not show
any alteration aer bioeld treatment as compared to control. In
endometrial adenocarcinoma, cytokines promote tumor development
by angiogenesis and mediating cell invasion. TNF-α, pro-inammatory
cytokine biomarker exists as both soluble and membrane-integrated
proteins activates signaling pathways, and is expressed on surface
epithelium in cancer and endometrial adenocarcinoma. It promotes
angiogenesis by the activation of NF-κB, a protein complex [35]. Apart
from TNF-α, IL-6 is another pro-inammatory cytokines whose level is
upregulated in endometrial adenocarcinoma, which is associated with
a poor prognosis [36]. Cancer research now proofed that dysregulation
in immune system contributes to metastasis and lead to cell death.
However, cancer progression is indicative by chronic inammation
which enhances angiogenesis and tissue metabolism results in cytokines
release. erefore, a signicant alteration of important inammatory
(NCCAM), now dened bioeld therapies in the subcategory of energy
therapies as one of the ve complementary medicine domains. Bioeld
is the name given to the electromagnetic eld that permeates and
surrounds living organisms. It is the scientically preferred term for
the biologically produced electromagnetic and subtle energy eld that
provides regulatory and communic ation funct ions within the organism.
Recent studies on cancer research suggest that instead of using other
complementary and alternate medicine, cancer patients received
highest benet with energy medicine [15]. e concept of human
bioenergy has its origin thousands of year back, till date many recent
bioeld therapies are in practice for their possible therapeutic potentials
such as enhanced personal well-being in cancer patient [16], improved
functional ability of arthritis patient [17], decreased pain and anxiety
[18]. However, many other signicant inhibitory eects on the cancer
cell model have been published which suggest that bioeld treatment
could be a new and eective treatment approach for cancer [19,20]. Mr.
Mahendra Trivedi’s unique bioeld treatment (e Trivedi’s eect®) has
signicantly studied in dierent elds. In agr iculture, change on growth,
characteristics, and yield of plants were reported aer bioeld treatment
[21,22]. It has also signicantly altered the crystalline and powder
characteristics of metals [23,24] and transformation of bioeld energy
has considerably altered the genotype and phenotypes of pathogenic
microbes against antimicrobials [25-27]. is experiment was designed
to evaluate the impact of bioeld treatment on cancer biomarkers in
endometrium and prostate cancer cell lines. Concentration of cytokines
(i.e. TNF-α and IL-6), interleukin-2 receptor (IL-2R), prostate specic
antigen (PSA), and free prostate specic antigen (FPSA) were estimated
by using ELISA assay on day 10 aer bioeld treatment as compared to
control.
Materials and Methods
Experimental design
Endometrium and prostate cancer cell lines, as stock stored cultures
were procured for the experiment from department of laboratory
medicine, P.D. Hinduja National Hospital & Medical Research Centre,
Mumbai. Enzyme-Linked Immuno-Sorbant Assay (ELISA) method
was used for estimation of cytokines levels. Important cancer diagnostic
biomarkers such as IL-6, TNF-α, PSA (third generation), FPSA, and IL-
2R of control and treated samples were estimated using ELISA kit as per
manufacturer’s instruction.
Bioeld treatment modalities
Endometrium and prostate cancer cell lines were taken for bioeld
treatment. e rst sets of both cancer cell line were considered as
control. No treatment was given to this set. e second sets of both
cancer cell line were handed over to Mr. Trivedi for bioeld treatment
under laboratory condition. Mr. Trivedi provided the bioeld
treatment through his energy transmission process to second sets of
samples without touching the samples. Aer treatment, both treated
samples were handed over in the same condition within an hour’s
time and stored at standard conditions for cytokines analysis aer
10 days. Both the control and treated samples were analyzed aer 10
days for dierence in cancer biomarker as per the standard protocols.
An optimum precautionary measure were taken in estimation of IL-
6, TNF-α, PSA (third generation), FPSA, and IL-2R throughout the
experiment. e dierences in tested cancer biomarkers level before
and aer the treatment were noted.
Measurement of diagnostic cancer biomarkers
ELISA was performed as per the manufacturer’s instructions in

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 255
biomarkers (IL-6 and TNF-α) in endometrium cancer cell line suggests
that bioeld treatment may aect metastasis, which results in alteration
of pleiotropic and multifunctional cytokine levels. is nding is the
rst evidence that bioeld therapy may alter the immune function in
endometrium cancer.
Bioeld treatment on prostate cancer cell line showed a signicant
reduction of as high as 98.8% in IL-6 level. Aer treatment, IL-6
concentrations showed less than 2 pg/ml as compared to control (165
pg/ml) in prostate cancer cells. Besides this, TNF-α level showed
an increase of 89.8% in bioeld treated group (i.e.7.59 pg/ml) as
compared to control (less than 4.0 pg/ml). Slight increase in level of
PSA was observed in bioeld treated prostate cell line as compared to
control (Table 1). However, level of FPSA and IL-2R did not show any
alteration aer bioeld treatment as compared to control. It suggests
that the bioeld treatment has signicant impact only on cytokines
level and not on receptors or antigens involved in metastasis. Above
cytokines have the major involvement in prostate cancer development
and elevated levels have been reported in prostate hyperplasia patients
[37]. IL-6 and TNF-α levels have strong correlation in serum with
hormone refractory prostate cancer as compared to normal cell [38].
Present study reported a signicant reduction in IL-6 concentration
and an increase in TNF-α level in prostate and endometrium cancer
cell lines aer bioeld treatment (Figure 1). e result of the present
study supports the already published literature with signicant bioeld
eect on cancer cell lines. is study results are in agreement with the
reported data [31-33] and verify the inuence of bioeld treatment
on in vitro growth of prostate and endometrium cancer cell line with
respect to cancer biomarkers level in control and treated samples.
Current cancer therapies have certain limitations, which include
serious side eects such as enormous toxicity, altered immune system,
and high treatment cost etc. Many ancient bioeld therapies for cancer
Figure 1: Percent change in cytokine level measured using ELISA assay on day 10 after bioeld treatment in (a) endometrium cell line (b) prostate cell line.
S. No. Biomarker Endometrium cell line Prostate cell line
Control Treated % change Control Treated % change
1IL-2R (U/ml) <50.0 <50.0 NC <50.0 <50.0 NC
2 IL-6 (pg/ml) 4.0 3.52 -12 165 < 2.0 -98.8
3TNF-α (pg/ml) 4.23 20.5 385 < 4.0 7.59 89.8
4 PSA (ng/ml) < 0.003 < 0.003 NC < 0.003 0.003 NC
5 FPSA (ng/ml) < 0.05 < 0.05 NC < 0.05 < 0.05 NC
NC: No change; pg/ml: picogram per milliliter; ng/ml: Nano gram per milliliter; U/ml: Unit per milliliter; IL-2R: Interleukin-2 receptor; IL-6: Interleukin-6; TNF-α: Tumor necrosis
factor alpha; PSA: Prostate specic antigen; FPSA: Free prostate specic antigen.
Table 1: Effect of bioeld treatment on cancer biomarker concentration in endometrium and prostate cancer cell lines.

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 256
are practiced around the world, among then Qigong therapy for cancer
is very popular and scientically studied [39,40]. erefore, Mr. Trivedi’s
bioeld treatment could be an eective and alternate approach for
cancer with no side eects. Research on bioeld therapies and its impact
on cancer growth have been continuously studied by researchers, which
might works as alternate and complementary medicine to prolong the
life of cancerous patients along with quality life.
Conclusion
Study results conclude that bioeld treatment has signicantly
altered the level of cytokines (IL-6 and TNF-α) in endometrium and
prostate cancer cell lines as compared to control. Mr. Trivedi’s bioeld
treatment showed a signicant reduction in IL-6 level in prostate
cancer cells (98.8%) and endometrium cancer cell (12%) as compared
to control. ere was also a signicant increase of TNF-α concentration
in both endometrium and prostate cancer cell lines. ese data suggest
that bioeld treatment has signicant impact in altering the cancer
biomarkers especially two cytokine levels in both cancer cell lines.
Acknowledgement
Authors gratefully acknowledged to support of Trivedi science, Trivedi
testimonials and Trivedi master wellness in this research work.
Conict of interest
The authors declare that they have no competing interests.
References
1. Mirkin S, Pickar JH (2015) Selective estrogen receptor modulators (SERMs): a
review of clinical data. Maturitas 80: 52-57.
2. Febbraro T, Lengyel E, Romero IL (2014) Old drug, new trick: repurposing
metformin for gynecologic cancers? Gynecol Oncol 135: 614-621.
3. Siu DF, Siu Neto J, Abrão MS, Favero G (2014) Lymphadenectomy in early
stage endometrial cancer: a critical review of the current literature. Tumori 100:
477-485.
4. Creasman WT, Odicino F, Maisonneuve P, Quinn MA, Beller U, et al. (2006)
Carcinoma of the corpus uteri. FIGO 26th Annual Report on the results of
treatment in gynecological cancer. Int J Gynaecol Obstet 95: S105-S143.
5. Randall ME, Filiaci VL, Muss H, Spirtos NM, Mannel RS, et al. (2006)
Randomized phase III trial of whole-abdominal irradiation versus doxorubicin
and cisplatin chemotherapy in advanced endometrial carcinoma: A gynecologic
oncology group study. J Clin Oncol 24: 36-44.
6. Siegel R, Naishadham D, Jemal A (2013) Cancer statistics, 2013. CA Cancer
J Clin 63: 11-30.
7. Huss WJ, Barrios RJ, Foster BA, Greenberg NM (2003) Differential expression
of specic FGF ligand and receptor isoforms during angiogenesis associated
with prostate cancer progression. Prostate 54: 8-16.
8. Joyce JA, Pollard JW (2009) Microenvironmental regulation of metastasis. Nat
Rev Cancer 9: 239-252.
9. Smith DS, Catalona WJ, Herschman JD (1996) Longitudinal screening for
prostate cancer with prostate-specic antigen. JAMA 276: 1309-1315.
10. Daiter E, Pampfer S, Yeung YG, Barad D, Stanley ER, et al. (1992) Expression
of colony-stimulating factor-1 in the human uterus and placenta. J Clin
Endocrinol Metab 74: 850-858.
11. Kayl AE, Meyers CA (2006) Side-effects of chemotherapy and quality of life in
ovarian and breast cancer patients. Curr Opin Obstet Gynecol 18: 24-28.
12. Alvarez Secord A, Havrilesky LJ, Bae-Jump V, Chin J, Calingaert B, et al.
(2007) The role of multi-modality adjuvant chemotherapy and radiation in
women with advanced stage endometrial cancer. Gynecol Oncol 107: 285-291.
13. Bruzzone M, Miglietta L, Franzone P, Gadducci A, Boccardo F (2004)
Combined treatment with chemotherapy and radiotherapy in high-risk FIGO
stage III-IV endometrial cancer patients. Gynecol Oncol 93: 345-352.
14. Barnes PM, Bloom B, Nahin RL (2008) Complementary and alternative
medicine use among adults and children: United States, 2007. Natl Health Stat
Report : 1-23.
15. Garland SN, Valentine D, Desai K, Langer C, Evans T, et al. (2013)
Complementary and alternative medicine use and benet nding among
cancer patients. J Altern Complement Med 19: 876-881.
16. Giasson M, Bouchard L (1998) Effect of therapeutic touch on the well-being of
persons with terminal cancer. J Holist Nurs 16: 383-398.
17. Peck SD1 (1998) The efcacy of therapeutic touch for improving functional
ability in elders with degenerative arthritis. Nurs Sci Q 11: 123-132.
18. Turner JG, Clark AJ, Gauthier DK, Williams M (1998) The effect of therapeutic
touch on pain and anxiety in burn patients. J Adv Nurs 28: 10-20.
19. Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, et al. (2013) Evaluation
of bioeld treatment dose and distance in a model of cancer cell death. J Altern
Complement Med 19: 124-127.
20. Chen KW (2008) Inhibitory effects of bio-energy therapies on cancer growth.
World Sci Tech Modern Trad Chin Med 10: 144-152.
21. Shinde V, Sances F, Patil S, Spence A (2012) Impact of bioeld treatment on
growth and yield of lettuce and tomato. Aust J Basic Appl Sci 6: 100-105.
22. Sances F, Flora E, Patil S, Spence A, Shinde V (2013) Impact of bioeld
treatment on ginseng and organic blueberry yield. AGRIVITA J Agri Sci 35:
22-29.
23. Dabhade VV, Tallapragada RR, Trivedi MK (2009) Effect of external energy
on atomic, crystalline and powder characteristics of antimony and bismuth
powders. Bull Mat Sci 32: 471-479.
24. Trivedi MK, Patil S, Tallapragada RM (2012) Thought Intervention through
bioeld changing metal powder characteristics experiments on powder
characterisation at a PM Plant, Springer Berlin Heidelberg, Editor: Wei Deng,
Lecture Notes in Electrical Engineering-Future Control and Automation 173:
247-252.
25. Trivedi MK, Patil S (2008) Impact of an external energy on Staphylococcus
epidermis [ATCC-13518] in relation to antibiotic susceptibility and biochemical
reactions-an experimental study. J Accord Integr Med 4: 230-235.
26. Trivedi MK, Patil S (2008) Impact of an external energy on Yersinia enterocolitica
[ATCC-23715] in relation to antibiotic susceptibility and biochemical reactions:
an experimental study. Internet J Alternat Med 6: 13.
27. Trivedi MK, Bhardwaj Y, Patil S, Shettigar H, Bulbule A (2009) Impact of an
external energy on Enterococcus faecalis [ATCC-51299] in relation to antibiotic
susceptibility and biochemical reactions-an experimental study. J Accord Integr
Med 5: 119-130.
28. Stephens JC, Larkins A, James RF, Rathbone BJ (1996) Production of a
monoclonal antibody against the 128 kDa (CagA) protein of Helicobacter pylori.
J Immunol Methods 190: 163-169.
29. Whitcomb BW, Perkins NJ, Albert PS, Schisterman EF (2010) Treatment of
batch in the detection, calibration, and quantication of immunoassays in large-
scale epidemiologic studies. Epidemiology 21 Suppl 4: S44-50.
30. Running A (2015) Decreased Cortisol and Pain in Breast Cancer: Bioeld
Therapy Potential. Evid Based Complement Alternat Med 2015: 870640.
31. Yount G, Patil S, Dave U, Alves-dos-Santos L, Gon K, et al. (2013) Evaluation
of bioeld treatment dose and distance in a model of cancer cell death. J Altern
Complement Med 19: 124-127.
32. Monzillo E, Gronowicz G (2011) New insights on therapeutic touch: a discussion
of experimental methodology and design that resulted in signicant effects on
normal human cells and osteosarcoma. Explore (NY) 7: 44-51.
33. Gronowicz GA, Jhaveri A, Clarke LW, Aronow MS, Smith TH (2008)
Therapeutic touch stimulates the proliferation of human cells in culture. J Altern
Complement Med 14: 233-239.
34. Renard N, Lienard D, Lespagnard L, Eggermont AMM, Heimann R, et al. (1994)
Early endothelium activation and polymorphonuclear cell invasion precede
specic necrosis of human melanoma and sarcoma treated by intravascular
high-dose tumour necrosis factor alpha (rTNF alpha). Int J Cancer 57: 656-663.
35. Seo KH, Lee HS, Jung B, Ko HM, Choi JH, et al. (2004) Estrogen enhances
angiogenesis through a pathway involving platelet-activating factor-mediated
nuclear factor-kB activation. Cancer Res 64: 6482-6488.

Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257. doi:10.4172/1948-5956.1000358
J Cancer Sci Ther
ISSN: 1948-5956 JCST, an open access journal Volume 7(7) 253-257 (2015) - 257
36. Slater M, Cooper M, Murphy CR (2006) Human growth hormone and interleukin-6
are upregulated in endometriosis and endometrioid adenocarcinoma. Acta
Histochem 108: 13-18.
37. Chen T, Wang LH, Farrar WL (2000) Interleukin 6 activates androgen receptor-
mediated gene expression through a signal transducer and activator of
transcription 3-dependent pathway in LNCaP prostate cancer cells. Cancer
Res 60: 2132-2135.
38. Drachenberg DE, Elgamal AA, Rowbotham R, Peterson M, Murphy GP (1999)
Circulating levels of interleukin-6 in patients with hormone refractory prostate
cancer. Prostate 41: 127-133.
39. Shen MX (1997) Qigong is an effective way to defeat cancer. China Qigong
Science 5: 8-10.
40. Chen K, Yeung R (2002) Exploratory studies of Qigong therapy for cancer in
China. Integr Cancer Ther 1: 345-370.
Citation: Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In
Vitro Evaluation of Bioeld Treatment on Cancer Biomarkers Involved in
Endometrial and Prostate Cancer Cell Lines. J Cancer Sci Ther 7: 253-257.
doi:10.4172/1948-5956.1000358