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Effect of harvest times on the yield and seed quality of French bean

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A two years field study was carried out in the research field and laboratory of Seed Technology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh during rabi season of 2008-10. The aim of this study was to determine the effect of five different dates of harvest viz. H1: 65 Days after emergence (DAE), H2: 70 DAE, H3: 75 DAE, H4: 80 DAE and H5: 85 DAE on the seed yield and viability of French bean. The experiment was laid out in randomized complete block design (RCB) with four replications. Results showed that the yield and yield contributing parameters significantly influenced the days to harvesting. The highest seed yields of 1.15 and 1.31 t ha-1 were obtained from the harvest of 75 DAE (Days after emergence) in 2008-09 and 2009-10, respectively. On the contrary, seed quality parameters like seed germination, root length and vigour index were also significantly influenced by harvest time. The highest seed germination percentage of 91.33 and 91.56 were recorded from the seed plots harvested at 75 DAE (pods are light brown with few yellow colour) both for the year 2008-09 and 2009-10, respectively. Similarly, the vigour indexes (12.49 and 11.64) were the highest in seed harvested at 75 DAE from the year of 2008-09 and 2009-10, respectively. This might be due to more food reserve and dry matter accumulation on the seed at later harvest. However, seeds harvested in 80 and 85 DAE were also found good for seed yield and seed quality.
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SAARC J. Agri., 13(1):01-13 (2015)
EFFECT OF HARVEST TIMES ON THE YIELD AND SEED
QUALITY OF FRENCH BEAN
M.R. Shaheb*1, M.N. Islam, A. Nessa, and M.A. Hossain2
Seed Technology Division, Bangladesh Agricultural Research Institute (BARI)
Joydebpur, Gazipur-1701, Bangladesh
ABSTRACT
A two years field study was carried out in the research field and
laboratory of Seed Technology Division, Bangladesh Agricultural
Research Institute, Gazipur, Bangladesh during rabi season of 2008-10.
The aim of this study was to determine the effect of five different dates of
harvest viz. H1: 65 Days after emergence (DAE), H2: 70 DAE, H3: 75
DAE, H4: 80 DAE and H5: 85 DAE on the seed yield and viability of
French bean. The experiment was laid out in randomized complete block
design (RCB) with four replications. Results showed that the yield and
yield contributing parameters significantly influenced the days to
harvesting. The highest seed yields of 1.15 and 1.31 t ha-1 were obtained
from the harvest of 75 DAE (Days after emergence) in 2008-09 and
2009-10, respectively. On the contrary, seed quality parameters like
seed germination, root length and vigour index were also significantly
influenced by harvest time. The highest seed germination percentage of
91.33 and 91.56 were recorded from the seed plots harvested at 75 DAE
(pods are light brown with few yellow colour) both for the year 2008-09
and 2009-10, respectively. Similarly, the vigour indexes (12.49 and
11.64) were the highest in seed harvested at 75 DAE from the year of
2008-09 and 2009-10, respectively. This might be due to more food
reserve and dry matter accumulation on the seed at later harvest.
However, seeds harvested in 80 and 85 DAE were also found good for
seed yield and seed quality.
Keywords: French bean, Seed maturity, Viability, Seed yield, Vigour
index
* Corresponding author email: smrayhan_bari@yahoo.com
1 On-Farm Research Division, Bangladesh Agricultural Research Institute (BARI), Sylhet-3100, Bangladesh
2 Horticulture Research Centre, Bangladesh Agricultural Research Institute, Joydevpur, Gazipur-1701
Received: 21.09.2014
2 M.R. Shaheb et al.
INTRODUCTION
French bean (Phaseolus vulgaris L.) is an important leguminous crop mostly
grown as green vegetable in Bangladesh. It is a nutritious vegetable commonly seen
in home gardens and cheap source of calcium, iron and vitamins. The immature
pod, tender parts and also dry beans are used as curry and French beans have a
possibility to meet a large share of vegetables demand in Bangladesh (BARI, 2007).
Singh et al. (1997) reported that French bean is a potential source of proteins,
carbohydrates and minerals. Mineral matter, crude fibre and ether extract are
concentrated in seed while crude protein and energy are stored in the cotyledons. In
Bangladesh, there is a vast scope to grow French bean both in the field and
homestead condition especially in Sylhet region. But higher production of crops
depends largely on the ability to integrate better crop management into the cultivation
systems. To boost yield, quality seed is essential both for vegetables and seed
production. Seed maturity, viability and storability are correlated with each other.
Early harvested seeds are immature and poorly developed, resulting in poor quality
that affects subsequent storability compared to seeds harvested at physiological
maturity. Delayed harvesting also results in the loss of yield due to shattering,
damage of seed and the risk of rain that affect seed quality. The quality of bean seeds
depends on many pre and post-harvest factors, such as the area of production,
cultivation techniques, seed maturity, harvesting, threshing, processing and storage
conditions. Greven et al. (2004) reported that timing of harvest is an important factor
since both seed immaturity and rewetting reduce seed quality. Kumar et al. (2002)
reported that seed yield and quality largely depends on the stage of maturity of crops.
Therefore, harvesting of seeds at the optimum stage of maturity is important as
harvesting either at early or late stage results in lower yields with poor quality seeds.
Dharmalingam and Basu (1990) reported that seed development and maturation study
is important to ensure good yield associated with viability, vigour and field
performance. Moisture content of harvested crops also affects seed quality.
Harvesting at high moisture content increases the chances of mycofloral infection on
seed, while harvesting at low moisture content increases mechanical damage to seed
(Yadav et al., 2005). Mehta et al. (1993) stated that chickpea attained maximum dry
matter accumulation (physiological maturity) when most pods were light brown with
few yellow green, thereafter, dry weight of seed decreased due to disruption of
vascular connection and utilization in various physiological and metabolic processes
like respiration.
Seed development is the period between fertilization and maximization of fresh
weight accumulation and seed maturation begins at the end of seed development and
continues till harvest (Mehta et al., 1993). The seed reaches its maximum dry weight
at physiological maturity and seeds should be harvested at this time to ensure their
quality in terms of germinability and vigour. If the seeds are retained on mother plant
after physiological maturity, physiological changes in seed may lead to formation of
hard seeds or off colour seeds in pulse crops. Attainment of physiological maturity is
YIELD AND SEED QUALITY OF FRENCH BEAN 3
a genotypic character which is influenced by environmental factors (Mahesha et al.,
2001a). Storability of seed is mainly a genetic character and is influenced by pre-
storage history of seed, seed maturation and environmental factors during pre and
post-harvest stages (Mahesha et al., 2001b). Greven et al. (2004) reported that later
sowing, higher plant populations, desiccation and earlier harvesting reduced seed size
of Dwarf French beans, but significant differences were found in germination and
seed vigour.
Muasya (2001) reported that both high temperature and less rainfall could
reduce seed quality of common bean. A number of studies revealed that high
temperature is more detrimental than limited rainfall. Unfavorable weather conditions
may reduce seed quality mainly through reducing the maximum quality attainable
during crop development. The seed quality in general did not change significantly
between physiological maturity (PM) and harvest maturity (HM), but in some cases
the proportion of viable seeds increased between PM and HM, especially when
ambient temperatures were relatively low (Muasya et al., 2008). Thus, harvesting of
seed crop at optimum stage of seed maturation is essential to obtain better seed
quality. There is hardly any literature available on appropriate maturity time (harvest
time) of French bean in Bangladesh condition where seed quality will be maximum
and that will affect the subsequent viability and storability of seed. Hence, the
present study was done with the following objective:
i) To find out appropriate harvest time on the seed yield and quality of
French bean and
ii) To investigate the relationship between seed maturity and viability with
harvest time in French bean.
MATERIALS AND METHODS
The trial was conducted at the research field and laboratory of Seed
Technology Division, Bangladesh Agricultural Research Institute, Joydebpur,
Gazipur during rabi season from November 2008 to March 2010. The experimental
treatments comprised of five different dates of harvesting viz., H1: 65 Days after
emergence (DAE- Department of Agriculture Extension), H2: 70 DAE, H3: 75 DAE,
H4: 80 DAE and H5: 85 DAE. The harvesting stages were characterized by deep
green with light yellow colour of pod (65 DAE), 50% green and 50% yellowing of
pods (70 DAE), light brown with few yellow colour pods (75 DAE), 90% brown
colour of pods (80 DAE) and 100% brown colour and dried pods (85 DAE),
respectively. Approximately 100% field emergence was recorded at 10 days after
sowing of seed. The French bean (also called Bush bean) variety BARI Jharsheem-1
was used for the trial. The experiment was laid out in randomized complete block
design (RCBD) with four replications. The unit plot size was 12 sq.m. The land was
fertilized with 23, 75, 75 and 20 kg ha-1 of N, P, K and S in the form of Urea, TSP,
MoP and Gypsum, respectively (BARI, 2006). Half of N and all other fertilizers were
applied at the time of final land preparation. The remaining N was applied at 30
4 M.R. Shaheb et al.
DAS. Seeds were sown in furrows @ 60 kg ha-1 in 30 cm rows on 22 and23
November 2008 and 2009, respectively. Before sowing, all seeds were treated with
Bavistin @ 0.2x ml kg-1 of seed. All intercultural operation viz., weeding (two times
one each at 15 and 40 DAE), thinning (1 time), irrigation (two times one each at 30
and 50 DAE) were done. The crop was harvested on February 3 (65 DAE), Feb 8 (70
DAE), Feb 13 (75 DAE), Feb 18 (80 DAE) and Feb 23 (85 DAE). Prior to harvest 5
plants were selected randomly from each plot and uprooted carefully for collecting
data on yield contributing characters viz. field emergence, 50% of flowering, plant
height and pods plant-1, and seeds pod-1. After harvesting the collected pods were
then sun dried until the moisture content reached 10-12%. Seed yield data collected
from individual plots were converted to per hectare of yield. The data on seed quality
parameters like seed germination (%), root and shoot length (cm), average seedling
dry weight (g) and vigour index (%) were then taken in the laboratory following
ISTA rules. The collected data were statistically analyzed following the ANOVA
technique using MSTAT-C software. The mean differences among the treatment
means were adjudged by Least Square Difference (Gomez and Gomez., 1984). Seed
vigour index was calculated by multiplying germination (%) and seedling length
(Abdul-Baki and Anderson, 1973). Seed germination was calculated by the following
formula (ISTA, 1999):
i) Vigour index (VI) = (MRL + MSL) x PG ..............................(A)
Where,
VI = Vigour index;
MRL=Mean root length (mm);
MSL= Mean shoot length (mm) and
PG = Percentage germination.
ii) Seed germination (%) = × 100 ...............(B)
RESULTS AND DISCUSSION
Results obtained from the two consecutive years of 2008-09 and 2009-10 are
presented in table 1-4 and figure 1-4. Two years (2008-10) average maximum and
minimum temperature and total rainfall in Gazipur are presented in figure 1 and 2.
From the graphs, it was observed that the average maximum temperatures were in the
month April in both the years (34oC) while the lowest minimum temperature was
recorded in middle of January which was just above 9oC. There was almost no
rainfall recorded during the growing period (November to February) of French bean
in both the years except in the last week of February where a very small amount of
precipitation (5 mm) was observed. However, rainfall was recorded in all other
months ranging from 5 mm to 160 mm.
No. of seed germinated
Total seed
YIELD AND SEED QUALITY OF FRENCH BEAN 5
Effect of harvest time on the seed yield and yield parameters of French Bean
Results revealed that there were significant differences on plant height, number
of pods plant-1, pod length, 1000 seed weight and seed yield both from 2008-09 and
2009-10 (Table 1-2 and Figure 3-6). The highest plant heights (38 and 37.04 cm)
were observed from the harvest at 70 DAE in 2008-09 and 2009-10 respectively,
while the lowest plant heights (28.30 and 32.02 cm) in both the years were obtained
from the harvest at 85 DAE (Table 1). The highest numbers of pods plant-1 (21.13
and 22.75) were recorded from the harvest at 85 DAE in both years. Crop harvested
at 65 DAE produced the lowest numbers of pods plant-1 (17.62 and 18.03) in 2008-09
and 2009-10, respectively. It was observed that the longest pod (15.08 and 15.60 cm)
were obtained from the harvest at 70 and 75 DAE, while the shortest pods (13.75
and 13.60 cm) were found from the harvest at 85 DAE in 2008-09 and 2009-10,
respectively (Table 2). It was noted that the number of seeds per pods were
significantly different in 2009-10, unlike in 2008-09. The highest number of seeds
per pods (628) was recorded from the harvest of 75 DAE from 2009-10 (Table 2).
Significantly, the highest 1000 seed weight of 213.80 g and 207.26 gm were
recorded from the harvest of 80 and 75 DAE, respectively in 2008-09 and 2009-10,
while the lowest 1000 seed weight (137.10 and 141.82 g) were obtained from the
harvest at 65 DAE in both the years (Figure 3 and 4). The highest seed yield (1.15
and 1.31 t ha-1) were recorded from the harvest at 75 DAE from 2008-09 and 2009-
10, respectively, while the lowest seed yield (0.64 and 0.75 tons) were harvested at
65 DAE in both years (Figure 5 and 6). Number of pods per plant was directly
related to seed yield. Therefore, as 75 to 85 DAE harvested plants beard higher
number of pods per plant, so it reflected on seed yield on theses harvesting time. The
increase in yield in 75 DAE compared to 65 DAE was 79.68% in 2008-09 and
74.66% in 2009-10 which might be attributed to increased pods per plant, seeds per
pod and 1000 seed weight (Rani and Kodandaramaiah, 1997). The result is in
conformity with the findings of Khatun et al. (2010). The optimum time of harvesting
of French bean was also determined by regressing the 1000 seed weight and seed
yield with harvesting dates (Figure 3-6). Regression co-efficient study revealed that
associations between harvest time and 1000 seed weight (r2= 0.695 and 0.478) and
with seed yield (r2= 0.84 and 0.593) showed highly significant positive correlations.
Singh and Lachanna (1995) reported that early harvested seeds were immature and
poorly developed compared to seeds harvested at physiological maturity. The present
findings are in agreement with the findings of Khatun et al. (2010)who observed
that most pods of chickpea harvested at light brown with a few yellow green stages
recorded the highest seed yield. Greven et el. (2004) reported that rain during harvest
may reduce seed quality, especially for seeds with <25% seed moisture content. This
indicates that seed quality can already be reduced by rewetting before harvest
maturity is reached. In field crops, maximum seed quality is gained at physiological
maturity at the end of seed filling (Egli, 1998). Padrit et al. (1996) reported that
delaying pea harvest could also cause the seed to become too dry and therefore more
6 M.R. Shaheb et al.
easily damaged. Kavak et al. (2012) observed that early and late harvests not only
decrease physical quality of seed lots but also decrease seed quality. Mehta et al.
(1993) asserted that cultivars of chickpea attained maximum dry matter accumulation
(physiological maturity) at H2 stage when most of the pods were appeared light
brown with few yellow in colour thereafter, dry weight of seed decreased because of
restricted supply of nutrients from mother plants to seed due to disruption of vascular
connection and utilization in various physiological and metabolic processes like
respiration etc. Thus, maximum seed quality of French bean may be obtained during
harvest at physiological maturity.
Effect of harvest time on the seed quality parameters of French Bean
Significant influences were observed among the different harvest times of
French bean on the seed quality parameters viz., seed germination, root length, shoot
length and vigour index in 2008-09 and 2009-10, and average seedling dry weight in
2008-09 while the other parameters did not show any significant variations (Table 3
and 4). Results revealed that the highest seed germination (93.78 and 94.12%) was
found from the harvest at 75 DAE in 2008-09 and 2009-10 respectively, while the
lowest seed germination (62.67 and 64.11%) was recorded from the harvest at 65
DAE in both years. A similar trend was observed regarding the root and shoot length
and average seedling dry weight (Table 3). The highest vigour index (2891) was
recorded from the harvest of 75 DAE that was statistically identical with the harvest
of 80 (2823) and 85 DAE (2825) from the year 2008-09. The same trend was also
observed in vigour index (VI), where the highest VI (3069) was recorded in the seed
harvested at 75 DAE followed by the harvest at 80 (2711) and 85 DAE (2712) in
2009-10 (Table 4).
The lowest values of vigour index (6.57 and 6.48) were obtained at 65 DAE for
year 2008-09 and 2009-10, respectively. The quality of bean seeds depends on many
pre and post-harvest factors, such as area of production, cultivation techniques, seed
maturity and harvest as well as threshing, processing and storage conditions of seeds.
Therefore, appropriate time of harvesting might be responsible along with other
factors for higher seed quality of french bean seed harvested at 75 to 85 DAE. The
seed lot showing a higher seed vigour index is considered to be more vigorous
(Abdul-Baki and Anderson, 1973). The results are in agreement with the findings of
Ayyub et al. (2007) who reported that physiologically mature seeds displayed better
viability than immature seeds. The results is also conformity with the findings of
Khatun et al. (2009) who asserted that lentil seeds collected at the stage when most of
the pods were light brown with a few yellow (H2 stage) recorded the highest
germination percentage, dry weight, root length, shoot length, seedling length and
vigour index (vigour index-I and vigour-Il).
YIELD AND SEED QUALITY OF FRENCH BEAN 7
CONCLUSION
Seed is the most valuable, basic and vital living input for increasing crop
production. It has been scientifically proved that quality seed alone can contribute to
the increase of yield by 15-20%. Therefore, quality seed production at appropriate
time and seed maturity are a must for successful crop production. However, the
present investigation revealed that the highest seed yield (1.15 and 1.08 t ha-1) of
French bean (BARI Jharsheem-1) was obtained from third harvest (75 DAE) while
pods are shown light brown with few yellow colour in both the years. Regarding seed
quality parameters, the study showed that French bean seeds harvested at 75 days
after emergence were better in seed quality. Maximum seed germination and vigour
index were also observed in seed harvested at 75 days of emergence. However, seeds
harvested 80 and 85 DAE were also found good for seed yield and seed quality. To
sum up, it can be concluded that harvesting of French bean seed at 75 to 85 days after
emergence would be physiologically matured seed that ultimately lead to the highest
seed quality as well.
REFERENCES
Abdul-Baki, A.A. and Anderson, J. D. 1973. Physiological and biochemical deterioration of
seed. In Seed Biology (II Ed): Kozlowski, T. T., Academic Press, New York, London.
pp. 283-315
Ayyub, C.M., Ziaf, K., Pervez, M. A., Rasheed, M. A. S. and Akhtar, N. 2007. Effect of seed
maturity and storability on viability and vigour in pea (Pisumsativum L.) seeds.In.
Proceedings: International symposium on prospects of Horticultural Industry in
Pakistan hosted by Institute of Horticultural Sciences, University of Agriculture,
Faisalabad (Dated on 28-30 March 2007). Pp.269-273
BARI, 2006.Agrotechnology handbook (Part-1). Bangladesh Agricultural Research Institute
(BARI), Joydebpur. Gazipur., p-337
BARI, 2007. Annual Research Report (2006-2007). Horticulture Research Centre.
Bangladesh Agricultural Research Institute (BARI), Joydebpur. Gazipur.p-68
Dharmalingam, C. and Basu, R. N. 1990. Seed development and maturation studies in
mungbean. Seed Research, 6: 101-109
Egli, D.B. 1998. Seed biology and the yield of grain crops. CAB International, Wallingford,
pp-178
Gomez, K.A. and Gomez, A.A. 1984. Statistical procedures for Agricultural Research. 2ndedn.
John Wiley and Sons, New York. p 194
Greven, M.M.; McKenzie, B.A., Hampton, J.G., Hill, M.J., Sedcole, J.R. and Hill, G.D. 2004.
Factors affecting seed quality in dwarf French bean (Phaseolus vulgaris L.) before
harvest maturity. Seed Science and Technology, 32(3): 797-811
ISTA, 1999. International rules for seed testing. Seed Science and technology. International
Seed Testing Association, Zurich, Switzerland 27: 155-199
8 M.R. Shaheb et al.
Matthews, S. 2012. Effects of Seed Moisture
Content and Threshing Methods on Bean (Phaseolus vulgaris L.)Seed Quality.
Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 7 (1):51-57, 2012
Khatun, A ., Kabir, G. and Bhuiya, M.A.H. 2009. Effect of harvesting stages on the seed
quality of lentil (Lens culinaris L.) during storage. Bangladesh Journal of Agricultural
Research, 34(4): 565-576
Khatun, A., Bhuiyan, M.A., Nessa, A. and Byazid Hossain, S.M.. 2010. Effect of harvesting
time on yield and yield attibutes of chickpea (Cicer arietinum L.). Bangladesh Journal
of Agricultural Research, 35(1): 143-148
Kumar, V., Shahidhan, S.D., Kurdikeri, M.B., Channaveeraswami A.S. and Hosmani,
R.M.2002. Influence of harvesting stages on seed yield and quality in paprika
(Capsicum annuum L.) Seed Research, 30(1): 99-103
Mahesha, C.R., A.S. Channaveeraswami., M.B. Kurdikeri., M. Shekhargouda and Merwade,
M.N. 2001b. Seed maturation studies in sunflower genotypes. Seed Research, 29(1):
95-97
Mahesha, C.R., Channaveeraswami, A.S., Kurdikeri, M.B., Shekhargouda, M. and Merwade,
M.N. 2001a. Seed maturation studies in sunflower genotypes. Seed Research, 29(1):
95-97
Mehta, C.J., Kuhad, M.S., Sheoran, I.S. and Nandwal, A.S. 1993. Studies on seed
development and germination in chickpea cultivars. Seed Research, 21(2): 89-91
Muasya, R. M. 2001. Crop physiological analysis of seed quality variation in common bean
(Phaseolus vulgaris L.). Wageningen Universiteit. Promotor: Prof. Dr. Ir. P.C. Struik,
co-promotor(en): Dr. Ir. W.J.M. Lommen & Prof. Dr. E.O. Auma. - Wageningen:
Muasya, 2001. - ISBN 90-5808-469-8. P-169. UR - http://edepot.wur.nl/194574. ID -
109883
Muasya, R.M, Lommen, W.J.M., Auma, A.U. and Struik, P.C. 2006. Relationship between
variation in quality of individual seeds and bulk seed quality in bean
(PhaseolusvulgarisL.) seed lots. NJAS - Wageningen Journal of Life Sciences, 54(1):
5-16
Muasya, R.M., Lommen, W.J.M., Muui, C.W. and Struik, P.C. 2008. How weather during
development of common bean (Phaseolus vulgaris
attainable seed quality. NJAS - Wageningen Journal of Life Sciences, 56(1/2):85-100
Padrit, J., Hampton, J.G., Hill, M.J. and Watkin, B.R 1996. The effects of nitrogen and
phosphorus to the mother plant on seed vigour in garden pea (PisumsativumL.) cv.
Pania. Journal of Applied Seed Production, 14: 41-45
Rani, B.P. and Kodandaramaiah, D. 1997.Response of soybean (Glycine max) to inoculation
with varying nitrogen levels. Indian Journal of Agronomy, 42(1): 135-137
Singh Jai, et aI. (1997). Legume Res., 20: 230-235
Yadav, S.K., Yadav, S., Kumar, P.R. and Kant, K. 2005. A critical overview of chickpea seed
technological research. Seed Research, 33(1):1-5
YIELD AND SEED QUALITY OF FRENCH BEAN 9
Table 1. Effect of harvest time on the field emergence, days to 50% flowering and
plant height of French bean
Treatments*
Field emergence
(%)
Days to 50%
flowering Plant height (cm)
2008-09
2009-10
2008-09
2009-10
2008-09
2009-10
H1 95.25 95.75 45.50a 46.05 35.72 a
36.40a
H 2 95.50 95.50 46.50 45.65 38.00 a
37.04a
H 3 95.00 95.50 45.75 45.85 35.85 a
35.19ab
H 4 95.00 96.25 46.50 46.34 29.55 b
33.62bc
H 5 95.00 96.75 47.00 46.49 28.30 b
32.02c
CV (%) 0.87 - 2.01 - 6.40 3.93
LSD(0.05) NS NS NS NS 4.206 2.110
* H1: 65 DAE (Days after emergence), H2: 70 DAE, H3: 75 DAE, H4: 80 DAE and H5: 85 DAE
Table 2. Effect of harvest time on the pods plant-1, pod length and seeds pod-1 of
French bean
Treatments*
Pods plant -1 (nos.) Pod length (cm) Seeds pods-1 (nos.)
2008-09
2009-10
2008-09
2009-10
2008-09
2009-10
H1 17.62d
18.03b
15.07a 15.33a 5.32 5.27b
H 2 18.92c
18.65b
15.08a 15.40a 5.85 5.47b
H 3 20.03b
20.56ab
14.15ab
15.60a 5.77 6.28a
H 4 20.91ab
20.90ab
14.63ab
14.01b 5.30 5.71ab
H 5 21.13a
22.75a
13.75b 13.60b 5.30 5.69ab
CV (%) 2.15
9.23
3.92 4.04 7.64 7.60
LSD(0.05) 0.080
2.868
1.119 0.921 NS 0.666
* H1: 65 DAE (Days after emergence), H2: 70 DAE, H3: 75 DAE, H4: 80 DAE and H5: 85 DAE
10 M.R. Shaheb et al.
Table 3. Effect of harvest time on the seed germination, root and shoot length of
French bean after harvest
Treatments*
Germination (%) Root length (cm)
RL-2
Shoot length (cm)
2008-09 2009-10
2008-09
2009-10
2008-09
2009-10
H1 62.67c 64.11c 8.39b 7.98c 18.57c 19.60c
H 2 74.89b 73.11b 8.52b 8.17c 19.42b 21.25b
H 3 93.78a 94.12a 9.59a 10.01a 21.25a 22.59a
H 4 92.89a 90.56a 9.58a 8.97b 20.81a 20.96b
H 5 92.56a 92.00a 9.26a 8.55bc 21.26a 20.91b
CV (%) 3.26 3.35 2.5 4.58 2.13 2.85
LSD (0.05) 5.121 5.218 0.3859 0.7531 0.8120 1.131
* H1: 65 DAE (Days after emergence), H2: 70 DAE, H3: 75 DAE, H4: 80 DAE and H5: 85 DAE
Table 4. Effect of harvest time on the root-shoot ratio, average seedling dry weight
and vigour index of French bean
Treatments*
Shoot-
Root ratio (cm)
Average seedling dry
weight (g) Vigour index (VI)
2008-09
2009-10
2008-09
2009-10
2008-09
2009-10
H1 2.213 2.457 0.11b 0.11 1687c 1769d
H 2 2.283 2.603 0.12ab 0.11 2092b 2153c
H 3 2.213 2.257 0.14a 0.13 2891a 3069a
H 4 2.177 2.343 0.14a 0.13 2823a 2711b
H 5 2.297 2.450 0.12ab 0.12 2825a 2712b
CV (%) 3.68 5.88 9.88
4.32 2.25 3.93
LSD (0.05) NS NS 0.019 NS 118.4 183.8
* H1: 65 DAE (Days after emergence), H2: 70 DAE, H3: 75 DAE, H4: 80 DAE and H5: 85 DAE
YIELD AND SEED QUALITY OF FRENCH BEAN 11
Figure1. Maximum and minimum temperature (0C) from November to June of 2008-10
at Joydebpur, Gazipur
Figure 2. Total rainfall (mm) from November to June of 2008-10 at Joydebpur,
Gazipur
0.00
5.00
10.00
15.00
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30.00
35.00
40.00
0.00
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Minimum Temperature (avg.) 2008-
09
Minimum Temperature (avg.) 2009-
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Maximum Temperature (avg.) 2008-
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Maximum Temperature (avg.) 2009-
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Bi-month total rainfall in Gazipur-2008-2010
0
40
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200
Date
Total Rainfall (mm) 2008-09
Total Rainfall (mm) 2009-10
12 M.R. Shaheb et al.
Figure 3. Effect of harvest time on 1000 seed weight of French bean (2008-09)
Figure 4. Effect of harvest time on 1000 seed weight of French bean (2009-10)
y = 125.5x
R² = 0.695
0
50
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250
65 DAE 70 DAE 75 DAE 80 DAE 85 DAE
Days to harvest
y = 122.2x
R² = 0.478
0
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65 DAE 70 DAE 75 DAE 80 DAE 85 DAE
Days to harvest
YIELD AND SEED QUALITY OF FRENCH BEAN 13
Figure 5. Effect of harvest time on the seed yield of French bean (2008-09)
Figure 6. Effect of harvest time on the seed yield of French bean (2009-10)
y = 0.682x
R² = 0.84
0.00
0.20
0.40
0.60
0.80
1.00
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65 DAE 70 DAE 75 DAE 80 DAE 85 DAE
Days to harvest
y = 0.676x
R² = 0.593
0.00
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65 DAE 70 DAE 75 DAE 80 DAE 85 DAE
Days to harvest
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