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Antioxidant and antibacterial activity of Aloe vera gel extracts
... [15,17] This antioxidant like AVES is a molecule which reduces the extent of oxidative destruction of biomolecules. [11,15] It breaks the chain by donating an electron to the free radical present in the system. It also forms a new radical bond more stable than the initial one or it removes ROS initiators by forming strong bond with reagents. ...
... AVES in the sample would result in the reduction of Fe 3+ to Fe 2+ by donating an electron. [11,15] In this study, the reducing ability of AVES was observed in the formation of Perl's blue The reducing power of Aloe Vera extracts. ...
... This indicates that the anti bacterial active Aloe Vera ingredients like anthraqunones are more soluble in acetone than methanol and ethanol. [11] This is due to the fact that the resistance and susceptibility of bacteria are varied with the solvent used for extraction and extraction condition. [11,16] This the anti bacterial activities of Aloe Vera in this study were evaluated with three organic solvent extracts and their inhibition zones were put in the order of AEAG The antibacterial activity of Aloe Vera against bacterial strains involved in the treatment of many type of skin infections such as acne, herpes and scabies. ...
The first objective of this study was to investigate the anti bacterial activities of Aloe Vera using methanol extract of Aloe Vera gel (MEAG), ethanol extract of Aloe Vera gel (EEAG) and acetone extract of Aloe Vera gel (AEAG). Their inhibition zones were put in the order of AEAG (21-32 mm) > Combination (12-16 mm) > EEAG (11-14 mm) > MEAG (0-6 mm). The minimum inhibition zone was observed with methanol extract i.e. 6 mm for Bacillus subtilis and no clear zone is formed for E. coli rather than intermediate zone. Thus, both E. coli and bacillus bacterial strains are more resistive against MEAG than AEAG and EEAG. The second objective of this study was to evaluate the anti-oxidant activities of AVES using reducing and chelating activities of Aloe Vera. The inhibition efficiency of Aloe Vera extract (AVES) increased from 21.5% up to 75.56% on increasing of its concentration from 5 mg/Kg to 40 mg/Kg. This result indicates that Aloe extract provided a natural antioxidant by scavenging free radicals.
... It has been proposed that the anti-oxidant activity of AV may be a major property of this plant being used in the treatment of several diseases. 27,40 In this study, we found that AV gel could significantly decrease the CP-induced oxidative stress together with ameliorating neuronal injury in hippocampus tissue. In the other hand, treatment with AV gel could lead to increase in anti-oxidative defense in hippocampus tissue through increasing GPx, SOD and CAT activities and reducing the MDA level. ...
... They have found that AV gel decreases the MDA levels and increases the SOD activity leading to oxidative stress reduction following ischemia. 24,40 The AV gel used in the current study contains a large amount of flavonoid and phenols. It has been reported that flavonoids augment memory performance stimulating neurogenesis and protect neurons versus oxidative stress. ...
... It has been demonstrated that administration of these compounds has a neuro-protective effect against neurotoxicity induced by CP without altering its anticancer activity. 29,40,41 Apoptosis process can be triggered by three major pathways including an extrinsic pathway involving death receptors, intrinsic pathways involving mitochondria and endoplasmic reticulum stress pathway. It has been demonstrated that CP can induct these three pathways; whereas, the mitochondria-mediated pathway is the most important one among these three pathways. ...
Cisplatin (CP) as an important chemotherapeutic drug is used for the treatment of various malignancies; but it has some side effects on central nervous system, in particular hippocampus. The present study was aimed to determine the protective effects of Aloe vera (AV) gel on CP-induced oxidative stress, apoptosis and neurons structure changes in the hippocampus of rats. Forty-eight rats were divided into six groups including control, CP (5.00 mg kg-1 per week; intraperitoneally), CP + AV (400 mg kg-1 per day; orally), CP + metformin (200 mg kg-1 per day; orally), AV (400 mg kg-1 per day; orally) and metformin (200 mg kg-1 per day; orally). At the end of treatment, brain samples were obtained for analysis of apoptotic genes expression and anti-oxidant markers as well as histological study. The results showed that CP caused an increase in malondialdehyde level and a decrease in glutathione peroxidase, superoxide dismutase and catalase levels in CP group compared to control. The AV gel could diminish oxidative stress in the hippocampus of CP group and it resulted in down-regulation of Bax, caspase-3 and caspase-8 and up-regulation of Bcl-2 in CP group. It could ameliorate degenerative changes in hippocampus after exposure to CP. Our results showed that AV gel ameliorated oxidative stress, apoptosis and neuronal loss in the hippocampus of rats under CP treatment.
... The gel content per leaf is lower in A. arborescens than in A. barbadensis [28], which makes it less attractive for commercial purposes; nevertheless, it is interesting because of its potential medicinal properties. Different studies indicate a good inhibitory effect of A. barbadensis on some opportunistic bacteria and fungi, including Staphylococcus aureus [16,22,[33][34][35][36][37][38][39], Escherichia coli [3,22,35,[38][39][40], Pseudomonas aeruginosa [16,22,[35][36][37][38][39][40][41], Pseudomonas fluorescens [3], Bacillus cereus [34], and Candida albicans [37,40], which were also tested in presented study. ...
... The gel content per leaf is lower in A. arborescens than in A. barbadensis [28], which makes it less attractive for commercial purposes; nevertheless, it is interesting because of its potential medicinal properties. Different studies indicate a good inhibitory effect of A. barbadensis on some opportunistic bacteria and fungi, including Staphylococcus aureus [16,22,[33][34][35][36][37][38][39], Escherichia coli [3,22,35,[38][39][40], Pseudomonas aeruginosa [16,22,[35][36][37][38][39][40][41], Pseudomonas fluorescens [3], Bacillus cereus [34], and Candida albicans [37,40], which were also tested in presented study. ...
... The gel content per leaf is lower in A. arborescens than in A. barbadensis [28], which makes it less attractive for commercial purposes; nevertheless, it is interesting because of its potential medicinal properties. Different studies indicate a good inhibitory effect of A. barbadensis on some opportunistic bacteria and fungi, including Staphylococcus aureus [16,22,[33][34][35][36][37][38][39], Escherichia coli [3,22,35,[38][39][40], Pseudomonas aeruginosa [16,22,[35][36][37][38][39][40][41], Pseudomonas fluorescens [3], Bacillus cereus [34], and Candida albicans [37,40], which were also tested in presented study. ...
Simple Summary
Antimicrobial resistance is one of the major threats to public health, and additional concerns are reduced efficacy and increased toxicity of synthetically derived drugs. Hence, it is all the more important to research new antimicrobials derived from natural sources. Aloe spp. have long been acknowledged in traditional medicine, as their ability of treating skin and digestive problems, wound healing, anti-inflammatory, antimicrobial and other promising properties are known. This study presents the content of various bioactive substances in samples of two Aloe spp., Aloe arborescens and Aloe barbadensis, and their enzymatic, antioxidant and antimicrobial activity. Obtained bioactive compounds with antimicrobial effect have a huge potential to inhibit the growth of microorganisms that are extremely susceptible to gaining resistance and could be used in versatile applications in the cosmetics, food, medical and pharmaceutical industries.
Abstract
Recently, the use of Aloe species has become very widespread. These are extensively used as a nutraceutical in a variety of health care products and food supplements. In addition, the occurrence of the quickly adaptable microorganisms, particularly bacteria, which can develop resistance to antibiotics, is a major problem for public health, and therefore, it is necessary to search for new antimicrobials. In our study, the content of total phenols, proanthocyanidins, and proteins in fresh and lyophilized samples of A. arborescens and A. barbadensis and their ethanol extracts was investigated. Furthermore, enzymatic and antioxidant activity of samples were studied. Since antimicrobial activity of fresh samples was determined in our latest research, a more detailed study of antimicrobial effectiveness of A. arborescens and A. barbadensis (lyophilized, extracts) was performed. Ethanol extracts in particular contain higher concentrations of bioactive substances and show the topmost antioxidant activity. The novelty of the study refers to the observation of industrially important enzyme activities such as α-amylase, cellulase, lipase, peroxidase, protease, and transglutaminase in the samples as well as the microbial growth inhibition rates determination (MGIR) at different concentrations of added aloe samples. All samples inhibited the growth of all tested microbial cells. MIC90 for A. arborescens and A. barbadensis were also determined in case of B. cereus, P. aeruginosa, P. fluorescens, and S. aureus. The results of our study tend to give credence to the popular use of both aloes in medicine and in the cosmetic, food, and pharmaceutical industries.
... The reduction of Fe 3+ has been described as an indicator of electron-donating activity which can demonstrate the antioxidant potential of the different phenolic compounds of Phyto origin (Gunathilake & Ranaweera, 2016). The reducing power is generally associated with the presence of reductones (Saritha et al., 2010), which has been shown to exhibit antioxidant potential by splitting the free radical chains by donating hydrogen atoms. Reductones can prevent peroxide formation by reacting with the precursors of peroxides. ...
... Ghimire, A. and Bhattarai, S. been described as an indicator of electron-donating activity which can demonstrate the 194 antioxidant potential of the different phenolic compounds of Phyto origin (Gunathilake & 195 Ranaweera, 2016). The reducing power is generally associated with the presence of 196 reductones (Saritha et al., 2010), which has been shown to exhibit antioxidant potential by 197 splitting the free radical chains by donating hydrogen atoms. Reductones can prevent 198 peroxide formation by reacting with the precursors of peroxides. ...
... The reduction of Fe 3+ has been described as an indicator of electron-donating activity which can demonstrate the antioxidant potential of the different phenolic compounds of Phyto origin (Gunathilake & Ranaweera, 2016;Juntachote & Berghofer, 2005). The reducing power is generally associated with the presence of reductones (Saritha et al., 2010), which has been shown to exhibit antioxidant potential by splitting the free radical chains by donating hydrogen atoms. Reductones can prevent peroxide formation by reacting with the precursors of peroxides. ...
The book contains papers presented in the "National Conference on Integrating Biological Resources for Prosperity" held at Biratnagar, Nepal.
... About 75 nutrients and 200 biologically active compounds including sugar, anthraquinones, saponins, vitamins, enzymes, minerals, lignin, salicylic acid and amino acids has been now reported as constituents of Aloe vera. Previous studies suggests that the organic extracts of Aloe vera gel possess potent in vitro antibacterial activity (Saritha et al., 2010) [3] . Though the information on the physiological properties, antioxidant potential and antimicrobial activity of the Aloe vera gel are known but the antimicrobial properties of the AgNPs synthesized using it are not thoroughly worked. ...
... About 75 nutrients and 200 biologically active compounds including sugar, anthraquinones, saponins, vitamins, enzymes, minerals, lignin, salicylic acid and amino acids has been now reported as constituents of Aloe vera. Previous studies suggests that the organic extracts of Aloe vera gel possess potent in vitro antibacterial activity (Saritha et al., 2010) [3] . Though the information on the physiological properties, antioxidant potential and antimicrobial activity of the Aloe vera gel are known but the antimicrobial properties of the AgNPs synthesized using it are not thoroughly worked. ...
... Broad spectrum antimicrobial activities of Aloe vera gel extracts has been reported (Saritha et al., 2010) [3] and silver when used in its nitrate form (AgNO 3 ) is known to induce antimicrobial effect, but when silver nanoparticles are used, there is a huge increase in the surface area available for the microbe to be exposed to. So, in present study attempt has been made to synthesize AgNPs using aqueous extract of Aloe vera gel and aqueous solution of AgNO 3 [12] reported the enhanced antibacterial activity of green synthesized nanoparticles using Piper longum compared to the extract support our results. ...
... The results of extraction yields are close to those of the work carried out by Saritha et al. (2010) who recorded an extraction yield equal to 28.14 %, and 3.05 % for the methanol and chloroform extracts respectively. According to the results obtained by Ejoba (2012) they recorded an extraction yield of 8.6 % for chloroform extract and 5.4 % for the water extract. ...
... Several factors can influence the polyphenol and flavonoid contents such as the development period or the age of the plant (Okamura et al., 1996;Park and Kwon, 2006;Rodriguez et al., 2010). In term of DPPH-scavenging potential of methanolic extract, our results are in accordance with the studies carried out by Saritha et al. (2010), the inhibition percentages of methanolic, acetone and chloroform extracts were 93.14 %, 74.03 %, 57.68 % respectively at 250 µg/mL, in this studies the methanol extract showed better radical scavenging activity than the other extracts. Anirban et al. (2013) have also reported higher DPPH scavenging activities for methanolic extract (IC 50 58 µg/mL).This result is in agreement with the statement, Aloe vera extracted in different solvents obtained highest DPPH inhibition in methanol extract (Saritha et al., 2010). ...
... In term of DPPH-scavenging potential of methanolic extract, our results are in accordance with the studies carried out by Saritha et al. (2010), the inhibition percentages of methanolic, acetone and chloroform extracts were 93.14 %, 74.03 %, 57.68 % respectively at 250 µg/mL, in this studies the methanol extract showed better radical scavenging activity than the other extracts. Anirban et al. (2013) have also reported higher DPPH scavenging activities for methanolic extract (IC 50 58 µg/mL).This result is in agreement with the statement, Aloe vera extracted in different solvents obtained highest DPPH inhibition in methanol extract (Saritha et al., 2010). In the metal chelating activity on ferrous ions, the results of our investigation are in accordance with those published earlier, which mentioned that the methanolic extract showed more chelating ability than acetone extract of Aloe vera leaves. ...
The aim of the study was to investigate the Aloe vera phytochemical compounds using HPLC–mass spectrometry and to evaluate their antibacterial, antioxidant, photoprotective and cytotoxic activities. The acetone extract, methanol extract and its four fractions: chloroform, ethyl acetate, n- butanol, aqueous were subjected to evaluate their antibacterial activity by Aromatogram method against S.aureus, B. cereus, E. coli, P. aeruginosa, A. baumanii. The minimum inhibitory concentrations (MIC) were determinated by the medium agar incorporation method. Then, the antioxidant properties of all extracts and fractions were evaluated by using Hydroxyl radical scavenging, Galvinoxyl radical scavenging and Phenanthroline assays. The photoprotective activity was measured by determination in vitro of sun protection factor. The cytotoxicity of extracts and fractions was tested by Brine shrimp lethality method. The yields of methanol, chloroform, ethyl acetate, n-butanol, aqueous, acetone extracts are respectively 20.56%±0.38, 3.4%±0.11, 1.58%±0.12, 14.16%±0.11, 13.56%± 0.78, 0.68%±0.50. By LC-MS analysis, 8 bioactive phytochemical compounds were identified. The antibacterial activity showed that the n-butanol fraction, acetone and methanol extracts had an effect against all the bacteria.. The MIC value ranged from 1.25 to 10 mg/mL. The chloroform fraction exhibited highest antioxidant activity in (OH•2) scavenging assay (IC50 value: 79.53±0.66 μg/mL) and in GOR scavenging assay (IC50 value: 80.18±1.21 μg/mL, the acetone extract exhibited highest antioxidant activity in phenanthroline assay (A0.50 value: 46.75±0.35μg/mL). Furthermore, the all extracts and fractions showed high photoprotective activity. The acetone, chloroform and ethyl acetate fractions have displayed significant effect against the brine shrimp larvae.
... The results of extraction yields are close to those of the work carried out by Saritha et al. (2010) who recorded an extraction yield equal to 28.14 %, and 3.05 % for the methanol and chloroform extracts respectively. According to the results obtained by Ejoba (2012) they recorded an extraction yield of 8.6 % for chloroform extract and 5.4 % for the water extract. ...
... Several factors can influence the polyphenol and flavonoid contents such as the development period or the age of the plant (Okamura et al., 1996;Park and Kwon, 2006;Rodriguez et al., 2010). In term of DPPH-scavenging potential of methanolic extract, our results are in accordance with the studies carried out by Saritha et al. (2010), the inhibition percentages of methanolic, acetone and chloroform extracts were 93.14 %, 74.03 %, 57.68 % respectively at 250 µg/mL, in this studies the methanol extract showed better radical scavenging activity than the other extracts. Anirban et al. (2013) have also reported higher DPPH scavenging activities for methanolic extract (IC 50 58 µg/mL).This result is in agreement with the statement, Aloe vera extracted in different solvents obtained highest DPPH inhibition in methanol extract (Saritha et al., 2010). ...
... In term of DPPH-scavenging potential of methanolic extract, our results are in accordance with the studies carried out by Saritha et al. (2010), the inhibition percentages of methanolic, acetone and chloroform extracts were 93.14 %, 74.03 %, 57.68 % respectively at 250 µg/mL, in this studies the methanol extract showed better radical scavenging activity than the other extracts. Anirban et al. (2013) have also reported higher DPPH scavenging activities for methanolic extract (IC 50 58 µg/mL).This result is in agreement with the statement, Aloe vera extracted in different solvents obtained highest DPPH inhibition in methanol extract (Saritha et al., 2010). In the metal chelating activity on ferrous ions, the results of our investigation are in accordance with those published earlier, which mentioned that the methanolic extract showed more chelating ability than acetone extract of Aloe vera leaves. ...
... The ammonia formed during this distillation was collected in a boric acid solution and determined through titrimetric dosage. The value of ammoniacal nitrogen was multiplied by 6.25 [42]. ...
... The results at the end of the storage period indicated that the CTR and the AVG-treatment lost 4.98% and 2.92%, respectively, while the AVG/HPMC and AVG/LEO treatments minimized weight loss (1.50% and 1.56%, respectively) by maintaining the water content in fresh-cut apple. In fact, this weight loss is probably caused by the water evaporation on the fruit surface and is a leading cause of quality loss, such as shriveling of the surface and decreased juiciness [6,42]. Nonetheless, there are not many studies available in the literature concerning the mixing of HPMC with Aloe vera gel and lemon essential oil to form edible or biodegradable films or packages, to compare with our data. ...
Recently, there is increasing use of edible and biodegradable films and packaging that are both environmentally friendly and functional for storage and market distribution. Fresh-cut ‘Fuji’ apples, harvested in an organic farm, were treated, using a spraying technique, with three new edible coatings based on Aloe vera gel (AVG—40% v/w) and in combination with natural additives: lemon essential oil (LEO—1% v/w) and hydroxypropyl methylcellulose (HPMC—0.1% v/w) and compared with untreated sample (CTR), the physicochemical and sensory characteristics and the proximate compounds were evaluated. During cold storage, weight loss, soluble solids content, and color of uncoated slices were reduced, while softening, ripening, browning, and acidity were accelerated. In contrast, the AVG/HPMC treatment significantly delayed the above parameters related to post-harvest quality loss, while the AVG/LEO treatment delayed the browning processes, maintaining an excellent color during cold storage. Concerning proximate compounds, the treatments did not alter their concentration in the fruit tissues. Sensory analyses revealed no detrimental effect on taste, aroma, or flavor. Our data evidenced the positive effect of Aloe vera gel in combination with LEO and HPMC on fresh-cut apple quality as an innovative and sustainable technique to maintain fresh-cut apple quality.
... Chelating activity of metals is based on the quantitative chelation of Fe 2+ ions by the reagent ferrozine, which leads to the formation of a complex with Fe 2+ ions. (Saritha et al., 2010). ...
One strategy used to discover new drugs for therapeutic use is the study of natural products. Rose Geranium, P. graveolens,is a plant that has long been used in traditional medicine for its many medicinal benefits. In this study, we present the first phytochemical study of Pelargonium graveolenscultived in Souk Ahras, Algeria. The volatile profile of the plant's leaves was analysed by GC/MS. More than 59 compounds were identified in the essential oils obtained from the leaves, representing 100% of the total oil weight. The main essential oil compounds were oxygenated monoterpenes, of which citronellol (26.69%) and geraniol (13.83%) were the main representatives. Radical neutralisation activity was measured by the DPPH method and IC50values and the result for iron-reducing activity was from 22.93±8.08 μg/mL and 18.79±2.89 μg/mL (leaves) for the essential oil respectively.Considering these findings, Pelargonium graveolensessential oils have the potential to be a good source of natural antioxidants
... За установяване на антиоксидантните свойства на Aloe vera гел (AGE), приготвени в метанол (MEAG), 95% етанол (EEAG), хексан (HEAG), ацетон (AEAG) и хлороформ (CEAG) са извършени тестове, при които е установено, че метаноловият (MEAG) и ацетоновият (AEAG) екстракти от Aloe vera гел притежават максимално свободни радикали (Saritha, Anilakumar, Khanum, 2010). ...
Настоящето проучване представя данни за биологичните и фармакологични свойства на растението Aloe vera. Доказано е, че в листата му са открити над 75 потенциално биологично активни съставни елемента - витамини, ензими, минерали, захари, липиди, сапонини, салицилова киселина и аминокиселини,
полизахариди, фенолни съединения и др., които определят неговото противовъзпалително, имуномодулиращо, хепатопротективно, антиоксидантно, антимикробно, антитуморно и антидиабетично действие. Описано е въздействието на тези физиологично-активни вещества върху човека, както и потенциалните възможности за използване на растението в борбата срещу редица заболявания.
... Te edible coating could enhance microbial safety of foods by reducing or preventing microbial infestation. Te AVG coating provides better barrier against infestation of Gram-positive than Gram-negative bacteria [52]. Additionally, it aids in minimizing the proliferation of Rhizopus stolonifer, Botrytis cinerea, and Penicillium digitatum [53]. ...
Edible coatings and active packaging have become more prevalent in response to changing consumption patterns and market trends to enhance the quality and safety of fresh products. In this work, we investigated the efect of aloe vera gel (AVG) coating and parafn wax-coated paperboard (PWB) packaging on the postharvest quality attributes of both grapes and apples during storage. Te fruits were coated with 50% AVG concentrations, and the inner wall of the corrugated paperboard was coated with parafn wax emulsion. Te grapes and apples were stored for 12 and 35 days, respectively, at ambient conditions (25 ± 3°C and 80-85% relative humidity). Te physicochemical properties, microbiological attributes, and decay incidence of the fruits were analyzed at intervals during storage. Both fruits treated with AVG and PWB packaging retained better qualities than the control at the fnal day of the storage period. Particularly, PWB packaging provided considerably superior quality from the control sample in terms of weight loss (≈54% and 32%), frmness (≈48% and 68%), and color diference (≈30% and 28%) for both grapes and apples. Tese fndings would introduce a novel approach for preserving the quality attributes of both climacteric and nonclimacteric fruits for a prolonged storage period at ambient temperature by PWB packaging and AVG coating.
... 13 Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, although many other species are also used for their therapeutic properties. Despite their wide spread usage, studies from different laboratories often report wide variations in the therapeutic bioactivities from Aloe spp., with some studiers reporting potent antibacterial activity, 14,15 yet other studies reporting a complete lack of activity. 16 Leaves from individual plants within the same species may have widely varying levels of the bioactive phytochemicals and thus wide variances in bioactivity. ...
... Daging daun lidah buaya (Aloe vera L.) bersifat antioksidan karena mengandung senyawa flavonoid. 5 Farmakologi in silico (juga dikenal sebagai terapi komputasi, farmakologi komputasi) adalah area yang berkembang pesat yang secara global mencakup pengembangan teknik untuk menggunakan perangkat lunak untuk menangkap, menganalisis, dan mengintegrasikan data biologis dan medis dari berbagai sumber. Lebih khusus, mendefinisikan penggunaan informasi ini dalam penciptaan model komputasi atau simulasi yang dapat digunakan untuk membuat prediksi, menyarankan hipotesis, dan akhirnya memberikan penemuan atau kemajuan dalam kedokteran dan terapi. ...
Aloe vera (Aloe vera L.) is a functional plant that is often found and has a wide range of health advantages. Antioxidants are chemical molecules that inhibit free radicals in the body, which can cause disease by generating oxidation events that destroy cell structure and function. The goal of this research is to see how bioactive chemicals from the aloe vera plant (Aloe vera L.) interact with antioxidant activity. The molecular docking method is combined with insilico analysis in this study, which employs four key applications: autodock tools, autodock vina, biovia discovery studio, and open babel. All amino acid residues in the bioactive compound Aloe vera (Aloe vera L.) act on the active site of the Human endothelial nitric oxide synthase (4D1O) receptor, according to the findings of this study. The binding energi of bioactive substances from aloe vera skin extract (Aloe vera L.) to the 4D1O receptor yielded promising results. Conclusion: Aloe vera (Aloe vera L.) extract bioactive component possesses antioxidant activity, which can particularly reduce or prevent free radical generation in the body. Keywords: Aloe vera (Aloe vera L.); Antioxidant; Human endothelial nitric oxide synthase (4D1O) receptor; Insilico. Abstrak: Lidah Buaya (Aloe vera L.) merupakan tanaman fungsional yang umum ditemui dan memiliki banyak manfaat bagi kesehatan. Antioksidan adalah senyawa kimia penghambat radikal bebas di dalam tubuh yang dapat menghambat terjadinya reaksi oksidasi dalam tubuh yang dapat merusak struktur dan fungsi sel sehingga menimbulkan penyakit. Penelitian ini bertujuan untuk mengetahui interaksi antara senyawa bioaktif dari tanaman lidah buaya (Aloe vera L.) terhadap aktivitas antioksidan. Penelitian ini menggunakan metode molecular docking dengan analisis secara insilico yang menggunakan 4 aplikasi utama: autodock tools, autodock vina, biovia discovery studio, dan open babel. Hasil penelitian ini menunjukkan bahwa semua residu asam amino pada senyawa bioaktif Lidah buaya (Aloe vera L.) bekerja pada sisi aktif reseptor Human endothelial nitric oxide synthase (4D1O). Hasil binding energi senyawa bioaktif ekstrak kulit lidah buaya (Aloe vera L.) terhadap reseptor 4D1O menunjukkan nilai yang bagus. Simpulan: Senyawa bioaktif ekstrak Lidah buaya (Aloe vera L.) memiliki aktivitas antioksidan yaitu secara spesifik dapat menekan atau menghambat aktivitas pembentukan radikal bebas di dalam tubuh. Kata kunci: Lidah buaya (Aloe vera L.); Antioksidan; reseptor Human endothelial nitric oxide synthase (4D1O); Insilico.
... Hence, the present study focused on whether A. fleurentiniorum can be used to reduce or prevent the teratogenic and histopathologic effects of DBP in pregnant rats. [25] . ...
The aim of this study was to evaluate the effects of the ethanolic extract of Aloe fleurentiniorume against dibutyl phthalate (DBP) toxicity in pregnant rats. Twenty healthy adult pregnant rats were divided into four groups: control group (c) was given 1 ml/kg bw corn oil on gestation days (GD) 7 and 14; T1 group received 1000 mg/kg bw ethanolic extract of A. fleurentiniorum on GD 7, 8, 14 and 15; TII group was administered with 2000 mg/kg bw DBP on GD 7 and 14; TIII-treated group was administered with 2000 mg/kg bw DBP + 1000 mg/kg bw of A. fleurentiniorum extract. On GD18, pregnant rats were necropsied, and the fetuses were removed, fixed in ethan ol (90%), eviscerated, and processed for skeletal staining. In addition, the livers, kidneys, ovaries, and uterus were collected for the histological examination. The results showed a significant decrease in the body weight gain of pregnant females that received DBP compared with the other groups. Moreover, there was a significant decrease in the relative uterine weight in all treated groups. Additionally, histopathological studies on the livers, kidneys, ovaries, and uterus of pregnant rats that received DBP (TII) revealed some alterations. In contrast, the administration of Aloe extract with DBP to pregnant females in the TIII group showed better amelioration of all the examined organs than t h e TII group. There was a significant increase in the number of resorbed fetuses in all treated groups. Furthermore, a highly significant decrease in fetal body weight and length was observed, and abnormalities in the morphological and skeletal structures were recorded in all treated groups. Preventive Effect…. Elham A.S.Al-Shaibani et al.
... Hence, the present study focused on whether A. fleurentiniorum can be used to reduce or prevent the teratogenic and histopathologic effects of DBP in pregnant rats. [25] . ...
The aim of this study was to evaluate the effects of the ethanolic extract of Aloe fleurentiniorume against dibutyl phthalate (DBP) toxicity in pregnant rats. Twenty healthy adult pregnant rats were divided into four groups: control group (c) was given 1 ml/kg bw corn oil on gestation days (GD) 7 and 14; T1 group received 1000 mg/kg bw ethanolic extract of A. fleurentiniorum on GD 7, 8, 14 and 15; TII group was administered with 2000 mg/kg bw DBP on GD 7 and 14; TIII-treated group was administered with 2000 mg/kg bw DBP + 1000 mg/kg bw of A. fleurentiniorum extract. On GD18, pregnant rats were necropsied, and the fetuses were removed, fixed in ethanol (90%), eviscerated, and processed for skeletal staining. In addition, the livers, kidneys, ovaries, and uterus were collected for the histological examination. The results showed a significant decrease in the body weight gain of pregnant females that received DBP compared with the other groups. Moreover, there was a significant decrease in the relative uterine weight in all treated groups. Additionally, histopathological studies on the livers, kidneys, ovaries, and uterus of pregnant rats that received DBP (TII) revealed some alterations. In contrast, the administration of Aloe extract with DBP to pregnant females in the TIII group showed better amelioration of all the examined organs than the TII group. There was a significant increase in the number of resorbed fetuses in all treated groups. Furthermore, a highly significant decrease in fetal body weight and length was observed, and abnormalities in the morphological and skeletal structures were recorded in all treated groups.
... The ABL extracts showed higher activity at 100 µg/mL in which methanolic extract showed activity at 59.73 ± 2.01% followed by ethanol (58.79 ± 1.20%), acetone (50.75 ± 1.50%), ethyl acetate (37.94 ± 1.27%), petroleum ether (34.88 ± 0.90%), and hexane (24.59 ± 2.35%). Similarly, the higher activity of DPPH was recorded in methanol and ethanol extract of aloe vera by Saritha et al. [108]. Previous report by Lopez et al. [109] stated that the soxhlet extracted methanol aloe vera sample showed 53 ± 2% inhibition. ...
Aloe barbadensis Miller (ABM) is a traditional medicinal plant all over the world. Numerous studies were conducted to exhibit its medicinal properties and most of them were concentrated on its metabolites against human pathogens. The current research work evaluates the attributes of different polar-based extracts (ethanol, methanol, ethyl acetate, acetone, hexane, and petroleum ether) of dried Aloe barbadensis leaf (ABL) to investigate its phytochemical constituents, antioxidant potential (DPPH, ABTS), phenolic, tannin, flavonoid contents, identification of bioactive compounds, and functional groups by gas chromatography–mass spectrometry (GC–MS) and fourier transform infrared spectroscopy (FT-IR) respectively, and comparing antibacterial efficacy against human pathogens, aquatic bacterial pathogens, and zoonotic bacteria associated with fish and human. The present results showed that the methanolic extract of ABL showed higher antioxidant activity (DPPH-59.73 ± 2.01%; ABTS-74.1 ± 1.29%), total phenolic (10.660 ± 1.242 mg GAE/g), tannin (7.158 ± 0.668 mg TAE/g), and flavonoid content (49.545 ± 1.928 µg QE/g) than that of other solvent extracts. Non-polar solvents hexane and petroleum ether exhibited lesser activity among the extracts. In the case of antibacterial activity, higher inhibition zone was recorded in methanol extract of ABL (25.00 ± 0.70 mm) against Aeromonas salmonicida. Variations in antibacterial activity were observed depending on solvents and extracts. In the current study, polar solvents revealed higher antibacterial activity when compared to the non-polar and the mid-polar solvents. Diverse crucial bioactive compounds were detected in GC–MS analysis. The vital compounds were hexadecanoic acid (30.69%) and 2-pentanone, 4-hydroxy-4-methyl (23.77%) which are responsible for higher antioxidant and antibacterial activity. Similar functional groups were identified in all the solvent extracts of ABL with slight variations in the FT-IR analysis. Polar-based solvent extraction influenced the elution of phytocompounds more than that of the other solvents used in this study. The obtained results suggested that the ABM could be an excellent source for antioxidant and antibacterial activities and can also serve as a potential source of effective bioactive compounds to combat human as well as aquatic pathogens.
Graphical Abstract
... Aloe aborescens Aloe Vera is the most versatile and nutritional store house nature favor to it by giving enormous nutrients and bioactive compounds [2,9]. It is a nutrient tower that contains around 75 nutrients and 200 active compounds including minerals, amino acids, enzymes and vitamins as summarized in Fig.2 [9][10][11] ...
The main objective of this study was to assess the metals level in Aloe Vera and in the soil where the Aloe Vera plant has grown in three sites, namely; Konso, Welaita and Arba Minch in southern region of Ethiopia using flame atomic absorption spectrometry (FAAS). The results of this study reveal that Aloe Vera plant has the ability to accumulate relatively higher amounts of K and Mn among the determined macro-and micro-elements, respectively. The mean levels of metal in Aloe Vera among three geographical areas in this study could be put in the following order: K (51.880 mg/Kg>> Ca (27.440 mg/Kg) > Mg (7.890 mg/Kg) > Na (2.335 mg/Kg) > Mn (2.083 mg/Kg) > Fe (2.043 mg/Kg) > Ni (2.033 mg/Kg) > Cr (1.670 mg/Kg) > Co (0.947 mg/Kg) > Cu (0.189 mg/Kg). But the metals level in soil are decreased in the order of: Ca (25.110 mg/Kg) > Mg (7.600 mg/Kg) > K (2.764 mg/Kg) > Na (2.330 mg/Kg) > Ni (2.240 mg/Kg) > Fe (2.157 mg/Kg) > Cr (1.700 mg/Kg) > Co (1.650 mg/Kg) > Mn (1.510 mg/Kg) > Cu (0.189 mg/Kg). Hence, the metal level in Aloe Vera are more dominant since plants absorb metal ions from different sources and then hyper-accumulated.
... 9 Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, although many other species are also used for their therapeutic properties. Despite their wide spread usage, studies from different laboratories often report wide variations in the therapeutic bioactivities from Aloe spp., with some studiers reporting potent antibacterial activity, 10,11 yet other studies reporting a complete lack of activity. 12 Leaves from individual plants within the same species may have widely varying levels of the bioactive phytochemicals and thus wide variances in bioactivity. ...
Figure 1: Eremophila macdonnelli F. Muell. (Family: Scrophulariaceae) is an endemic Australian plant. Several
Eremophilia spp. have been used in traditional First Australian medicine to treat a wide variety of ailments.1,2 The
genus consists of more than 200 species that grow in semi-arid and arid regions of Australia. Multiple Eremophila
spp. are used as traditional medicines by the First Australians in the areas in which they grow to treat diverse
conditions including uses as antibacterial, antifungal, antiviral, antioxidant, anti-diabetic, and anti-inflammatory
therapies, as well as for their cardio-protective properties. The antibacterial properties of Eremophila spp. have been
relatively well studied and the several bioactive terpenoids have been identified. This photograph was taken in the
Australian Arid Lands Botanic Garden, Port Augusta, Australia, in January 2021 by Ian Cock
... Aloe vera leaf powder was prepared according to the method described by Saritha et al. (2010). The extract of each powdered part of Aloe vera plants was used for phytochemical tests and to identify the constituents, standard procedures were carried out as described by Trease and Evans (1989) and Sofowora (1993). ...
Background: Aloe vera has been used by mankind for thousands of years in folk medicine for its therapeutic properties. The rich phytochemistry of Aloe vera has revealed great potential for improving the performance of livestock and poultry. Mizoram lies in the Indo-Myanmar subtropical forest region and is a biodiversity hotspot with many endemic species. The well-heeled biodiversity provides enormous assets and sustainable contribution of medicinal plants and thus leads to frequent use of plants as medicine. Moreover, it was reported that the nutrient composition of the plant depends on the soil quality of the locality. Therefore, the study was carried out to evaluate the phytochemical and nutrient composition of Aloe vera in an agro-climatic condition of Mizoram. Methods: The physical characteristics of fresh Aloe vera leaf i.e. length, width, thickness, apparent volume, leaf weight, gel weight and percent gel recovery were evaluated by using standard protocols. The nutrient composition of Aloe vera leaf powder i.e. moisture, crude protein, ether extract, crude fibre, carbohydrate, total ash, energy level and minerals i.e. calcium, phosphorus, zinc, copper, selenium, magnesium and chromium contents were estimated by using standard methods. The extract of each powdered part of Aloe vera plants was used for phytochemical tests and to identify the constituents, standard procedures were carried out. Result: The physical characteristics of fresh Aloe vera leaf i.e. length, width, thickness, apparent volume, leaf weight, gel weight and percent gel recovery were 562.72±6.32 mm, 83.55±4.46 mm, 25.33±4.05 mm, 298.44±7.32 cm 3 , 340.28±6.32 g, 180.23±9.39 g, 55.66±1.07%, respectively. The nutrient composition of Aloe vera leaf powder i.e. moisture, crude protein, ether extract, crude fibre, carbohydrate, total ash, the energy level was 91.12%, 8.75%, 2.4%, 16.8%, 52.0%,12.5%, 238 kcal, respectively and minerals i.e. calcium, phosphorus, zinc, copper, selenium, magnesium and chromium content was 2532.0, 870.1, 41.8, 0.57, 40.1, 8310.0 and 4.5 ppm, respectively. Aqueous and ethanol extraction of Aloe vera confirmed the presence of steroids, alkaloids, glycosides, carbohydrates, tannins and tarpenoid. In conclusion, the physical properties of Aloe vera revealed that the weight of the Aloe vera correlates with the gel portion irrespective of the leaf volume. Apart from therapeutic uses of Aloe vera, it can also use for the synthesis of complex chemical substances.
... Single-cell gel electrophoresis (SCGE) or Comet Assay method was used in our study to determine the effects of genotoxic agents on cells and to examine the damages that might occur in the DNA of cells [19]. The simplicity of the Comet Assay and its use in all kinds of tissues/cells increases the applicability of the test [20]. Lifespan of the D. melanogaster is affected by genotoxic agents which is a quantitative feature of phenotypic variation in natural populations based on both genetic and environmental components [21]. ...
... The in vitro antibacterial properties of methanolic extract of Aloe vera gel extract were investigated against various common pathogenic bacteria. Aloe vera gel extract could be same as a new source of natural antioxidant with potential applications for reducing the levels of lipid oxidation and oxidative stress [19]. The inhibitory potential of AVG against -amylase, -glucosidase, and pancreatic lipase activity in vitro was investigated. ...
Aloe a miracle plant obtained from many species namely aloe vera, aloe barbadensis, aloe perryi, aloe ferox and various
other species. Aloe vera the Indian species cultivated in India and other species are cultivated in Africa. Aloe is a cactuslike
plant that grows in hot, dry climates. Aloe plant can withstand drought conditions and can grow without rainfall. Aloe
produces two substances, gel and latex. Aloe gel is a clear, jelly-like substance found in the inner part of the aloe plant
leaf. Aloe latex comes from the plant's skin and is yellow in color. Some aloe products are made from the whole crushed
leaf, so they contain both gel and latex. Aloe vera plant has enormous activities in treatment of various skin diseases such
as acne, burns, psoriasis, etc. and used in herbal cosmetics. Aloe gel is used in many cosmetic preparations as face wash
and herbal creams etc. since the potency, efficacy, safety is more toxicity and side effects are comparatively less. Aloe is
used in gum diseases in, dental problems and dental plaque etc. Aloe gel has anti-diabetic activity, used in treatment of
glaucoma, and can treat vision problems. It is also used in hepatitis, osteoarthritis, varicose veins, weight loss etc. aloe has
tremendous other activities. This review includes various uses, medicinal properties, pharmacological actions, various
extracts of these Aloe species are traditionally used and their application used to cure, arthritis, inflammation, immunity,
diabetes, hyperlipidemic, antioxidant, atherosclerosis, coronary heart diseases, laxative, antibacterial, antifungal,
antiviral, wound healing and antitumor effect. Since aloe has been reviewed in many journal and articles, this review is
compiled from recent article of aloes. Hence aloe is an invaluable gift of nature and a miracle plant. Aloe is a medicinal
plant from the past, present and future and the invaluable activities present in this plant leads us to promising activities
for the research and development for finding new entities.
... This may be due to the fact that in plant minerals are found in complexed form and not in free form. Such a high amount of iron in acetone extract may be due to its good capacity of capturing iron and forming chelates with it 16 . ...
This study reports quantitatively ethanol, acetone and water extracts of Aloe saponaria for total phenols, flavonoids, mineral (Cu, Mn, Zn, Fe) contents and antioxidant activity. Maximum concentration of total phenols (0.019 mg GAE g-1 in water extract), flavonoids (3.60 mg CE g-1 in water extract) and Cu, Mn, Zn and Fe in ethanol, water, water and acetone extracts respectively, were observed. Best antioxidant activity (47.1%) was shown by ferric thiocyanate method in water extract and the highest antiradical activity (75.0%) by diphenyl-1-picrylhydrazyl free radical scavenging method was shown by water and ethanol extracts. A significant linear correlation was observed between the total phenols and antioxidant activity of plant extracts.
... In addition, FRAP method also contributes in quality control assessment, product differentiation, and development [66,83,84]. In this assay, antioxidant compounds in samples (fruits, vegetables, grains etc.), reduce oxidation state of Fe 3+ to Fe 2+ by donating an electron [85]. Measured levels of FRAP in the bulbs and leaves of all varieties are shown in Table 4. Relatively, bulbs of V1 planted at Kalar Kahar had significantly high (p < 0.05) ferric ion reduction power, followed by Lahore and Swabi (415.1 ± 10.7, 373.5 ± 16.5, 271.2 ± 27.6 mg GAE/100 g, respectively) on dry weight basis. ...
Genetic diversity and Agro-climatic conditions contribute significantly to the agronomic and morphological features of the food plant species, and their nutraceutical potential. The present study was intended to evaluate the impact of growing conditions on total phenolic and total flavonoid contents, and in vitro antioxidant potential in the bulbs and leaves of onion varieties planted under diverse environmental conditions. Standard analytical methods were used to quantify total phenolic content (TPC), total flavonoid content (TFC), and free radicals’ scavenging/antioxidant capacity. The impact of climatic and soil conditions was assessed using statistical tools. In general, onion varieties cultivated at three different locations viz. Kalar Kahar, Lahore and Swabi exhibited significant variations in TPC and TFC, and antioxidant activities. The bulbs and leaves of Mustang (V1) variety planted at Lahore and Swabi had significantly (p < 0.05), high levels of TPC (659.5 ± 6.59, and 631.1 ± 8.58 mg GAE/100 g, respectively). However, leaves of Red Orb (V2) and bulbs of Mustang (V1), and Golden Orb (V6), harvested from Kalar Kahar depicted the highest concentration of TFC (432.5 ± 10.3, 303.0 ± 6.67, and 303.0 ± 2.52 mg QE/100 g DW, respectively). Likewise, bulbs of V1 planted at Kalar Kahar, Lahore and Swabi exhibited maximum inhibition of DPPH, ABTS, and H2O2 radicals (79.01 ± 1.49, 65.38 ± 0.99, and 59.76 ± 0.90%, respectively). Golden Orb (V6) harvested from Lahore had the highest scavenging of OH radical (67.40 ± 0.09%). Likewise, bulbs of V1 variety planted at KalarKahar and Swabi had significant capacity to scavenge ferric ions (415.1 ± 10.6 mg GAE/100 g DW), and molybdate ions (213.7 ± 0.00 mg AAE/100 g DW). Conversely, leaves of Amazon (V8), planted at Lahore and Swabi depicted significant levels of DPPH, ABTS, H2O2 radical scavenging (90.69 ± 0.26, 63.55 ± 1.06, 51.86 ± 0.43%, respectively), and reduction of ferric ions (184.2 ± 6.75 mg GAE/100 g DW). V6 leaves harvested from Lahore and that of Super Sarhad (V3) from Swabi showed the highest inhibition of OH radical (61.21 ± 0.79%), and molybdate ions (623.6 ± 0.12 mg AAE/100 g DW), respectively. Pearson correlation and principal component analysis revealed strong relationships of climatic conditions, soil properties and elevation with TPC, TFC and free radicals’ scavenging potential in the bulbs and leaves of onion varieties. The variations in the total phenolic and flavonoid contents, and antioxidant potential of different varieties, and their associations with climatic and soil factors revealed the complexity of the growing conditions and genetic makeup that imposed significant impacts on the synthesis of secondary metabolites and nutraceutical potential of food and medicinal plant species.
... 14 Shea butter (Butter extracted from the nut of Vitellaria paradoxa) is used as non-oily emollient and skin moisturizer and also has sun screening properties. 15 aloe vera gel (Aloe barbadensis Miller liliaceae) is an established antioxidant 16 and antiaging 17 substance used in cosmetic industry. ...
UV absorption properties of bioactive agents has lead to their screening and development to provide photo protection. Safranal is one such secondary plant metabolite obtained from Saffron (Crocus sativus) and reported to possess antioxidant and antisolar properties. The objective of this research was to design a suitable delivery system for the topical delivery of Safranal and to develop broad spectrum polyherbal sun protection cream with mild photoprotection.
Safranal loaded Solid Lipid Nanoparticles (SLN) were formulated using probe sonication technique. The effect of variables like lipid concentration, surfactant concentration and stirring time were studied using central composite design using Design Expert 7.0 (Stat-Ease, Inc, USA). Particle size analysis of prepared SLN revealed the particles in the range of 460nm-980nm (F9). Entrapment efficiency was found between 88% and 99% . SLN was further characterized by techniques like DSC, FTIR and TEM. These SLN were combined with zinc oxide, pearl powder, Pterocarpus santalinus; a natural colorant with skin whitening effect and dispersed in a dermatological acceptable carrier with excellent skin nourishing properties. Other natural ingredients namely Almond oil, Hen egg oil and Aloevera gel were also incorporated due to their Sun protection properties. Evaluation of sunscreen cream by transmittane method showed good texture, excellent rheological properties, optimum pH and stability. The developed product showed broad spectrum of sunscreen protection with SPF 9.22, UV-A ++ and *** Boot Star Rating. The significant inhibitory activity of Safranal on matrix metalloproteinases (MMPs) analyzed by biochemical Investigation method and a higher SPF established that this bioorganic molecule is a strong photoprotective agent. Designing of Solid lipid nanoparticles and incorporation of other traditional ingredients in the recipe augmented its antisolar property and provided an all natural sunscreen.
... Flavonoids are also reported to have good antioxidant activity [51]. Our results are in good accordance with the findings that ethanol extract of Aloe vera leaf has more antioxidant activity than n-hexane and aqueous extract [20,43]. ...
Ethnomedicinally, Galium elegans is a valuable species used to cure many indigenous ailments in Asia. This study explored the phytochemical, antioxidant, biofilm inhibition and cytotoxic properties of G. elegans extracts. The results showed that the ethanol extract was enriched in phenolic contents (151.12 ± 0.02 mg GAE/g) and demonstrated potent antioxidant activity (IC50 57.95 μg/mL). Biofilm inhibition of E. coli showed that petroleum ether extract was more potent in the inhibition of biofilm (65.75 ± 0.3%). The nominal cytotoxicity of all the tested crude extracts ranged from 12.02 ± 0.40 to 32.03 ± 0.64%, revealed its safe usage as ethnomedicine. In thrombolytic study, only the acetone extract showed a very small anti-fibrinogen potential (3.07 ± 0.25). In conclusion, G. elegans qualifies preliminary in vitro evaluation as a potential source for antioxidant and biofilm inhibitory compounds. These findings license additional investigation to separate major bio-compounds from the extracts of G. elegans. Rezumat Galium elegans este o specie utilizată în medicina tradițională din Asia. Acest studiu a evaluat proprietățile fitochimice, antioxidante, de inhibare a biofilmului și proprietățile citotoxice ale extractelor de G. elegans. Rezultatele au arătat că extractul etanolic a fost bogat în constituenți fenolici (151,12 ± 0,02 mg GAE/g) și a demonstrat o activitate antioxidantă puternică (IC50 57,95 μg/mL) iar extractul în eter de petrol a fost mai eficient în inhibarea biofilmului (65,75 ± 0,3%). Citotoxicitatea nominală a tuturor extractelor brute testate a variat de la 12,02 ± 0,40 la 32,03 ± 0,64%. În studiul trombolitic, doar extractul în acetonă a arătat un potențial antifibrinogenic foarte mic (3,07 ± 0,25). În concluzie, G. elegans reprezintă o sursă potențială de compuși antioxidanți și inhibitori ai biofilmelor și sunt necesare investigații suplimentare pentru a separa bio-compușii majori din extractele de G. elegans.
... The spines around the leaves were removed using a knife and the thick epidermis was removed to collect the solid mucilaginous gel with a sterilized spoon. Gel was cut into small pieces and blended in an electric blender for 3 min (Saritha et al., 2010). ...
The present study was designed to compare the side effects of Cisplatin and 5-Fluorouracil with that of Aloe perryi leaves gel extracts. Twenty female albino rats (100 – 110 g) were used and assigned to 4 groups: group (I); control which received 1000 mg/kg distilled water for 30 days, group (II); intraperitoneally with 1.5 mg/kg of Cisplatin and 20 mg/kg 5-FU for 14 days; group (III) treated by Aloe aqueous extract (1000 mg/kg for 30 days), group (IV) treated by Aloe methanolic extract (1000 mg/kg for 30 days), at the end of the experiment they were anesthetized and liver and kidney were removed for histopathological studies. There was a decrement in bodyweight and relative liver and kidney weight recorded in Cisplatin and 5-FU group when compared with Aloe aqueous and methanolic extracts and control group. Histopathological investigation of the liver and kidney tissue of the control, Aloe aqueous, and methanolic extracts groups were found to be normal in structure. However, Congestion, hemorrhage, inflammatory cells infiltration, dilatation, fatty changes, vacuolation, fibrosis, and pyknosis showed histopathological changes in liver tissue of group II (intraperitoneally with Cisplatin and 5-FU for). Moreover, the histopathological analysis showed that the glomerular shrinkage, dilation, degeneration, congestion, vacuolation, and tubular shrinkage were the kidney histopathological changes in group II. It was concluded that Aloe perryi aqueous and methanolic leaves extracts have no side effects on liver and kidney tissues of rats as compared to Cisplatin and 5- fluorouracil.
... In the present study, the latex extract of A. irafensis showed significant antioxidant activity as shown by the DPPH free radical scavenging assay. This result is in agreement with a previous study which reported that Aloe species such as A. vera and A. inermis possessed high DPPH free-radical and superoxide-radical scavenging activities [10,23]. ...
Objectives: The objectives of the study were to determine the phytochemical constituents and to assess the antibacterial, antioxidant, and wound-healing properties of the methanol extracts of Aloe irafensis. Methods: Methanol extracts of A. irafensis's latex, gel, and green skin were screened for their phytochemical constituents. All three extracts were investigated regarding their antibacterial potential using disc diffusion and microdilution assays, and their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay. Histological analyses of wound healing areas were performed following the administration of the latex extract in male albino rats. Results: The methanol extracts of A. irafensis revealed the presence of carbohydrates, steroids, phenols, tannins, and anthrones. The latex extract showed greater inhibition zones against Staphylococcus aureus and Pseudomonas aeruginosa (24 and 17 mm, respectively) with minimum inhibitory concentration values of 1.25 and 2.50 mg/ml, respectively. The latex extract showed the highest antioxidant activity (IC 50 of 65.54 µg/ml), followed by green skin extract (IC 50 of 89.48 µg/ml). The latex extract significantly accelerated the rate of wound healing in rats (p<0.01), compared to the reference control fucidin ointment. Histological findings showed remarkably less scar width at wound closure site in the latex extract-treated wounds. Granulation tissue contained fewer inflammatory cells and more fibroblasts in wounds treated with the latex extract compared to those treated with the vehicle. Conclusion: A. irafensis latex extract is a potential source of bioactive compounds that can be exploited for antioxidant, antibacterial, and wound healing purposes.
... In the present study, the latex extract of A. irafensis showed significant antioxidant activity as shown by the DPPH free radical scavenging assay. This result is in agreement with a previous study which reported that Aloe species such as A. vera and A. inermis possessed high DPPH free-radical and superoxide-radical scavenging activities [10,23]. ...
Objectives: The objectives of the study were to determine the phytochemical constituents and to assess the antibacterial, antioxidant, and wound-healing properties of the methanol extracts of Aloe irafensis. Methods: Methanol extracts of A. irafensis's latex, gel, and green skin were screened for their phytochemical constituents. All three extracts were investigated regarding their antibacterial potential using disc diffusion and microdilution assays, and their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay. Histological analyses of wound healing areas were performed following the administration of the latex extract in male albino rats. Results: The methanol extracts of A. irafensis revealed the presence of carbohydrates, steroids, phenols, tannins, and anthrones. The latex extract showed greater inhibition zones against Staphylococcus aureus and Pseudomonas aeruginosa (24 and 17 mm, respectively) with minimum inhibitory concentration values of 1.25 and 2.50 mg/ml, respectively. The latex extract showed the highest antioxidant activity (IC 50 of 65.54 µg/ml), followed by green skin extract (IC 50 of 89.48 µg/ml). The latex extract significantly accelerated the rate of wound healing in rats (p<0.01), compared to the reference control fucidin ointment. Histological findings showed remarkably less scar width at wound closure site in the latex extract-treated wounds. Granulation tissue contained fewer inflammatory cells and more fibroblasts in wounds treated with the latex extract compared to those treated with the vehicle. Conclusion: A. irafensis latex extract is a potential source of bioactive compounds that can be exploited for antioxidant, antibacterial, and wound healing purposes.
... In the present study, the latex extract of A. irafensis showed significant antioxidant activity as shown by the DPPH free radical scavenging assay. This result is in agreement with a previous study which reported that Aloe species such as A. vera and A. inermis possessed high DPPH free-radical and superoxide-radical scavenging activities [10,23]. ...
Objectives: The objectives of the study were to determine the phytochemical constituents and to assess the antibacterial, antioxidant, and woundhealing properties of the methanol extracts of Aloe irafensis.
Methods: Methanol extracts of A. irafensis’s latex, gel, and green skin were screened for their phytochemical constituents. All three extracts were investigated regarding their antibacterial potential using disc diffusion and microdilution assays, and their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay. Histological analyses of wound healing areas were performed following the administration of the latex extract in male albino rats.
Results: The methanol extracts of A. irafensis revealed the presence of carbohydrates, steroids, phenols, tannins, and anthrones. The latex extract showed greater inhibition zones against Staphylococcus aureus and Pseudomonas aeruginosa (24 and 17 mm, respectively) with minimum inhibitory concentration values of 1.25 and 2.50 mg/ml, respectively. The latex extract showed the highest antioxidant activity (IC50 of 65.54 μg/ml), followed by green skin extract (IC50 of 89.48 μg/ml). The latex extract significantly accelerated the rate of wound healing in rats (p<0.01), compared to the reference control fucidin ointment. Histological findings showed remarkably less scar width at wound closure site in the latex extract-treated wounds. Granulation tissue contained fewer inflammatory cells and more fibroblasts in wounds treated with the latex extract compared to those treated with the vehicle.
Conclusion: A. irafensis latex extract is a potential source of bioactive compounds that can be exploited for antioxidant, antibacterial, and wound healing purposes.
... Referencias de uso medicinal: Sobre la acción antibacteriana, se ha probado que frente a Staphylococcus aureus tiene una ligera actividad inhibidora (Martínez et al., 1996;Saritha et al., 2010;Chen et al., 2010). En el área de dermatología y odontología se le otorga importancia en su acción contra microorganismos responsables de la caries dental y enfermedades periodontales (Lacerda et al., 2013;Bonilla y Jiménez, 2016). ...
La presente obra contiene resultados de la investigación de tesis de Karla Anahí Ramírez Ovando, egresada de la Maestría en Ciencias en Producción Agropecuaria Tropical de la UNACH. Este documento fue sometido a dictámenes a doble ciego, con evaluadores externos, conforme lo establecen los criterios académicos y de rigor para la comunicación científica de calidad; los expedientes obran en la Dirección de Investigación, de la Dirección General de Investigación y Posgrado de la UNACH.
En este catálogo se presenta la diversidad de especies de plantas medicinales, usadas comúnmente en beneficio de la salud de las personas, en las localidades de Querétaro, Francisco I. Madero, Jerusalén y Montebello Altamira, del municipio de Ángel Albino Corzo (Chiapas, México). La información que se brinda, fue generada en gran parte con la investigación de tesis titulada “Estudio de los conocimientos tradicionales sobre plantas medicinales en patios de comunidades en Ángel Albino Corzo, Chiapas”, defendida en el programa de Maestría en Ciencias en Producción Agropecuaria Tropical de la Universidad Autónoma de Chiapas. Aquí se comparte el nombre científico, nombre común, partes utilizadas, propiedades medicinales, algunas contraindicaciones e ilustraciones de plantas identificadas en los patios domésticos de esas poblaciones campesinas; por lo que se destaca que este catálogo se realizó con la participación de la gente de las localidades referidas.
... The DPPH radical scavenging method [75][76][77][78], which is widely used in antioxidant activity studies and determined rapidly with UV-Visible spectrophotometer, was studied at different concentrations for the antioxidant activities of the BBHMP, NA and Pt (II) complex. The IC 50 value is the concentration of the sample causing a 50 % reduction in DPPH concentration and and is obtained from linear regression of the concentration plots of the tested compounds against the mean percentage of antioxidant activity [79]. The lower the IC50 value, the higher the antioxidant activity of the samples tested. ...
In the presented study, Pt(II) complex including 4-Bromo-2,6-bis-hydroxymethyl-phenol (BBHMP) and nicotinamide (NA) was synthesized and structurally analyzed by using spectral and thermal analysis methods. The BBHMP and its Pt(II) complex involving BBHMP and NA were investigated for their antimicrobial, cytotoxicity, gen expression and antioxidant properties. The antimicrobial activity results showed that the platinum complex displayed moderate antibacterial and antifungal activities. The cytotoxicity of the BBHMP and platinum complex were determined against human prostate (DU145) and breast (MCF7) cancer cell lines by applying MTT assay. Cytotoxicity results suggest that the Pt(II) complex exhibited moderate cytotoxicity against growth of used cancer cell lines when compared with the reference drug cisplatin. Gen expression results proved that Pt(II) complex is a special bioactive chemical constituent and potential anticancer agent. In addition, the complex demonstrated important antioxidant activity.
... Azoxymethane induced oxidative stress in rats was tested to review the consequences of oral feeding with burn plant gel extract, and therefore the results showed that the hepatic glutathione and acid levels reduced by AOM were restored to normal levels with AGE feeding [35]. The antioxidative properties aged prepared in methanol, 95% ethanol, hexane (hexane extract of burn plant gel or HEAG), acetone (AEAG), and chloroform (chloroform extract of burn plant gel or CEAG) were tested by Saritha V et al, and it had been revealed that MEAG and AEAG possessed maximum 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicaland superoxide radical-scavenging activities [36]. also suggest that ROS is beneficial in cancer development, dietary antioxidants, and endogenous [37]. ...
Aloe vera is used for medicinal purposes since ancient times. The botanical name is Aloe barbadensis miller. It belongs to the Liliaceae family. It is also called the healing plant or the silent healer, because of its wound and burn healing properties. Latex and gel are the two basic products of Aloe leaves. The latex and gel contain biologically active components.Polysaccharides contained in the gel of the leaf attribute most of the health benefits associated with Aloe Vera. Aloe was available as pills, ointments, jellies, sprays, drinks, etc. The important application aloe vera was wound healing, treating burns, anti-inflammatory, antidiabetic, anti-cancer, anti-ulcer, protection against skin damage from x-rays, lung cancer, intestinal problems, increasing HDL, reducing LDL, reducing blood glucose in diabetics, fighting against acquired immune deficiency (AIDS), allergies, etc. The aloe vera plant, its biological properties are briefly reviewed in this article.
... 11 Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, although many other species are also used for their therapeutic properties. Despite their wide spread usage, studies from different laboratories often report wide variations in the therapeutic bioactivities from Aloe spp., with some studiers reporting potent antibacterial activity, 12,13 yet other studies reporting a complete lack of activity. 14 Leaves from individual plants within the same species may have widely varying levels of the bioactive phytochemicals and thus wide variances in bioactivity. ...
... 11 Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, although many other species are also used for their therapeutic properties. Despite their wide spread usage, studies from different laboratories often report wide variations in the therapeutic bioactivities from Aloe spp., with some studiers reporting potent antibacterial activity, 12,13 yet other studies reporting a complete lack of activity. 14 Leaves from individual plants within the same species may have widely varying levels of the bioactive phytochemicals and thus wide variances in bioactivity. ...
Petalostigma triloculorae (commonly known as quinine bush)
unripe fruit and leaves. Petalostigma is an Australian Euphorbiaceae
genus which consists of 7 species. They grow to between 2 and 10 metres
in height and have bright orange fruit (when ripe). Petalostigma species
were used extensively by indigenous Australians to treat a myriad of
bacterial, fungal and viral diseases.1 P. pubescens bark and fruit decoctions
were used extensively by Australian Aborigines as an antiseptic and to
treat sore eyes. Fruit were also held in the mouth to relieve toothache.1
Despite its common name, there is no scientific evidence to support the
presence of quinine in the fruit or leaves (the common name is presumably
due to the extremely sharp bitter flavour of the fruit). Recent studies
have confirmed the antibacterial, antifungal and antiviral activity of
extracts of the leaves and fruit of this plant.2,3 Interestingly, it has recently
been reported that Petalostigma spp. extracts not only have inherent
antibacterial activity, but they also contain synergising compounds
that allow conventional antibiotics to function, even in bacterial strains
otherwise resistant to their actions.4 This photograph was taken at
Griffith University, Brisbane, Australia in November 2020 by Dr. Ian Cock.
... For S. aureus (Figure 7), inhibition zones of 3.0 ± 0.1 cm and 3.7 ± 0.2 cm were found for FG/AV1 and FG/AV4, respectively, in contrast to the control FG film which did not show any inhibition zone, as expected. This antibacterial activity could be attributed to the presence of polyphenols and other antioxidants in AV with ability to produce the precipitation of cell membrane proteins or having non-specific interactions with them [18,57,58], in agreement with the high TPC and antioxidant activity values obtained in this work. On the other hand, the acemannan present in AV has been reported to be also responsible for antibacterial activity [2]. ...
Edible films based on the addition of Aloe Vera gel (AV) into fish gelatin (FG) with antimicrobial and functional properties for food packaging applications were proposed in this work. AV showed an amphiphilic nature by infrared spectroscopy, high total phenolics content (TPC), antioxidant activity and thermal stability with an initial degradation temperature of 174 ± 2 °C. Mannose and glucose were quantified as main monosaccharides whereas the linkage composition study confirmed the presence of acemannan as main active polysaccharide. Three different formulations were obtained by the casting technique and the addition of AV contents of 0, 1 and 4 wt.% to FG, showing films with 4 wt.% of AV the best performance. The addition of AV did not significantly affect mechanical and barrier properties to oxygen and water vapour. However, some structural changes were observed by infrared spectroscopy and the obtained glass transition temperature values due to intermolecular interactions that increased the hydrophilicity and solubility of the resulting FG/AV films. A higher thermal stability was observed in films with AV content increasing the initial degradation and oxidation onset temperatures. An antimicrobial activity against S. aureus was also observed for FG/AV films. The addition of AV into FG could be proposed as a potential effective material to increase the postharvest quality of packed fruits and vegetables by retarding the microbial growth and extending the shelf-life of these food products.
... As showed in Table 1, the highest percentage of yield was obtained in ethanol extract (580 mg/100 g), followed by petroleum ether (186 mg/100 g), chloroform (134 mg/100 g), and ethyl acetate extract (120 mg/100 g) in A. adigratana Reynolds gel. Saritha et al. [14] reported that the percentage yield of extractive values of crude 95% ethanol extracts of Aloe vera gel was 154.1 mg/100 g. Then, the results of the present study were parallel with the results of other. ...
Objective: Aloe adigratana Reynolds is an herb that belongs to the Xanthorrhoeaceae family. It is native species to Tigray region in Northern Ethiopia and locally known as ere, it is called the healing plant because of its wound and burn healing properties. The present study was carried out to perform the phytochemical screening with different organic solvents and evaluate the in vitro antioxidant activities of ethanolic extract of A. adigratana Reynolds gel. Methods: The present study was to assess the qualitative phytochemical analysis and in vitro antioxidants potential of ethanolic extract of A. adigratana Reynolds gel by 1,1-diphenyl-2-picrylhydrazyl, reducing power assay, hydroxyl radical, nitric oxide, and superoxide radical scavenging activities using standard methods. Results: Qualitative phytochemical screening revealed that ethanolic extract of A. adigratana Reynolds gel contained a maximum number of secondary metabolites such as flavonoids, tannins and phenolic compounds, and terpenoid then other solvent extracts; further, the in vitro antioxidant activity of A. adigratana Reynolds ethanolic gel extract showed significant superoxide radical and nitric oxide scavenging activity with 76.67% and 65.39% inhibition with half maximal inhibitory concentration = 36.94 μg/ml and 62.35 μg/ml, respectively. Conclusion: It can be concluded that ethanolic extract of A. adigratana Reynolds gel had significant antioxidant properties, due to the presence of polyphenolic compounds, and could serve as a potential source of natural antioxidants, for the development of therapeutic antioxidant drugs.
KEY WORDS: Phytochemical, Aloe adigratana Reynolds gel, Antioxidants, 1,1-diphenyl-2-picrylhydrazyl
... Currently, one of the most studied natural matrices of edible coatings is the gel extracted from Aloe vera leaves; its gel is rich in bioactive compounds that have been widely used for their medicinal and therapeutic properties [24], including anthraquinones; aloin α and β; hemodin; aloe-mannan (polysaccharide); lecithin; almost all essential amino acids (20 out of 22); many vitamins (A, B1, B2, B6, B12, C and E); mineral salts (zinc, magnesium, manganese, selenium, calcium and iron); acetylsalicylic acid and choline amylase. As a coating, it has been extensively used in concentrations between 50% and 100% in products such as nectarines [25], mangoes [26], apples [27,28], papayas [29] and peaches [30] in order to reduce respiration rates, ethylene production, weight loss and microbial load due to its antibacterial and antifungal activity [31,32]. However, as demonstrated by Farina et al. [27,29], Aloe vera gel-based edible coatings without the addition of additives (such as polysaccharides or lipids) show no improvement in fresh-cut fruits compared to other edible coatings added with these natural additives. ...
In recent years, the market for minimally processed fruit has increased. Fresh-cut fruits are characterized by a short shelf life due to the processing phases that accelerate the ripening courses. The aim of this work is to analyze the effect on the quality of fresh-cut Hayward kiwis of three different edible coatings based on (1) Aloe vera gel, (2) Aloe vera gel + hydroxypropyl methylcellulose and (3) Aloe vera gel + lemon essential oil. Fruit firmness, weight loss, color, soluble solids content, titratable acidity, microbial load and sensory analysis were evaluated as fresh after 2, 4, 7 and 10 days. Aloe vera gel and Aloe vera gel + lemon essential oil maintained the best values, as they acted as a barrier to gas exchange and further reduced the microbial load. These results were confirmed by sensory analysis: Aloe vera gel + hydroxypropyl methylcellulose does not alter the natural taste of kiwi slices, Aloe vera gel + lemon essential oil gives the characteristic taste of lemon essential oil and Aloe vera gel gives an herbaceous taste. The Aloe vera gel, in combination with these additives, maintains the ability to preserve the quality of fresh-cut kiwifruit.
... It's used for treatment of wounds, burns and constipation from thousands of years [7]. It has pharmacological properties such as hypoglycaemic effect on diabetic mellitus, antibacterial, antifungal, anticancerous, antiinflammatory, immune-stimulant, and antioxidant [8]. Aloe juice has bitter taste and contains aloin, anthreaquinone, isobarbaloin, emodin, ester of cinnamic acid that play an important role in treatment of cancer, ulcer, and diabetes. ...
Aloe is a very popular plant, and present study aimed to correlate the flavonoids and their antimicrobial activity, antioxidant activity and nitric oxide scavenging activity. So, phenolic content and flavonoids were determined and studied in vitro their antimicrobial activity, antioxidant activity and nitric oxide scavenging activity with well diffusion method, minimum inhibitory concentration and scavenging by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and nitric oxide radicals (NO.) respectively with reference of Ascorbic acid as standard. The phenolic content was analysed by Folin Ciocalteu's phenol reagent and flavonoids with aluminium chloride. Phenolic content and flavonoids contents were 16.971mg tannic acid equivalent/g and flavonoids was 21.84mg Querecetin/g of extract powder respectively of Aloe leaves. The MIC value of extracts ranges between 0.95μg – 1.24μg against Gram-positive and Gram-negative bacteria. The flavonoids inhibited significantly DPPH radical with IC50 value of 893μg as compared to standard IC50 - 714μg. IC50 of nitric oxide is 973μg as compared to standard IC50 -500μg. These results represent the contents of phenolic components and flavonoids in Aloe and their correlation with antimicrobial activity, antioxidant activity and nitric oxide scavenging activity.
... Among the most studied plant genera is Aloe, which features in most articles on poultry health management. The bioactivity (anti-inflammatory, antioxidant, antimicrobial and immune modulatory activities) of Aloe extracts explains the widespread and successful use of the Aloe species in avian ethnomedicine (Agarry and Olaleye 2005;Im et al. 2010;Saritha et al. 2010;Devaraj and Karpagam 2011). There is still a lot more to do for the many plant species that have been identified from surveys to screen them and truly establish their pharmacological value. ...
An outline of the status of research and development of ethnoveterinary medicine in Zimbabwe is given in this chapter. Ethnomedicinal plants and practices have always been part of the management of animal diseases and other health problems in the country. Most rural folks still rely on these practices to treat diseases affecting all livestock species. However, formal ethnoveterinary research is still an emerging field in the country with most studies to date focusing on documenting plants used in management of different livestock health problems. Out of the identified plants, only few have been tested for efficacy in vitro and even fewer in vivo. This is notwithstanding the massive potential of developing pharmaceutical drugs and pesticides from plants with proven pharmacological or pesticidal activity. Several factors have made navigating this field difficult including poor acceptability of ethnotechnologies among the elite and policy makers, inadequate funding for research, poor equipment in scientific laboratories and the technical expertise to lead the research among other issues. This has often been worsened by the non-performing economic environment. There is, therefore, a lot that can be done to support a vibrant and robust research and development program on ethnoveterinary products in Zimbabwe.
The correlation between the high incidence of oro-dental pathologies (periodontitis, oral cancer) and the increased level of ROS in the human body have led to the need to use alternative treatments, namely to the use of active principles from medicinal plants that have a considerable antioxidant effect, thus reducing the risk of producing these oro-dental pathologies. In this sense, some of the bioactive compounds present in medicinal plants such as turmeric acid extracted from Curcuma longa, polyphenolic compounds extracted from Camellia sinensis, apigenin extracted from Moringa oleifera, timolol extracted from Thymus vulgaris, etc. are already used in oral care products, giving consumers the opportunity to capitalize on the therapeutic properties of natural plant extracts. Although many medicinal plants exhibit remarkable antioxidant properties, observed in vitro studies, when switching to in vivo studies these antioxidant properties are no longer similar due to interference between physiopharmacological processes such as absorption, metabolism, excretion. Therefore, additional studies are needed to prove even more clearly the antioxidant capacities of medicinal plants and implicitly their applicability in the prevention of oro-dental pathologies.
Ayurveda is the most popular traditional system among these and this indigenous system originated from India. Kumaryasava (KS) is a very popular arista in our state, probably due to their taste, alcoholic content, medicinal uses and physiological importance. The quality assessment of ayurvedic formulation is of prime importance in justifying their acceptability when compared with the modern system of medicine. A study reported that the therapeutic efficacy of herbal formulation always depends on its method of preparation and hence, there is need to develop a formulation with statistical optimization. The constituents essential for preparation of KS involve dravya, dravadravya, sandhaneeya dravya, prakshepa Dravya and madhura dravya. The other ingredients in formulation were selected based on in silico molecular docking for the antioxidant and hepatoprotective properties. In this article attempt is made to describe the screening of Kumaryasava formulation for optimization of the concentration of sweetening agent, fermentation duration and fermentation temperature for alcohol generated and selection of various excipients that affect formulation efficacy. According to the preliminary screening study it was observed that the sugar concentration 35 to 40%, fermentation time 30 days for ghataki flowers and fermentation temperature 25℃ has reported considerably significant results for alcohol content analyzed by gas chromatography. The preliminary screening parameters of KS formulation will be applied effectively for the development of formulation with a statistical approach.
Fresh Aloe vera plant leaves were brought from botanical garden and sample was identified and brought to the laboratory in the Department of Zoology, Patkar-Varde College, Goregaon (W), Mumbai. 50 grams of leaves were then grounded with 50ml of distilled water in sterilized pestle and mortar. The yield was being calculated based on weight of the extract compared to the weight of the pulp of the leaves. Forty eight (48) Sprague-dawley rats (average weight 150-250 g) of either sex were used for the experiment. The drugs ETH and PAS drug and Aloe vera juice were given to respective groups daily for 28 days. At the end of study animals were dissected and various tissues/ glands were processed for their histological studies. From the histology study, it was found that the anti-tuberculosis drugs ETH and PAS induced toxicity in male and female Sprague-dawley rats by disturbing the histological structure from minimal to mild changes in the histoarchetecture of lungs, Heart and Kidneys of male and female Sprague-dawley rats either independently or in combination. It was also found that when the anti-tuberculosis drugs ETH and PAS co-administered with Aloe vera juice found amelioration of the histoarchetecture of lungs, Heart and Kidneys of male and female Sprague-dawley to normal. It was also found that, the rats treated with ETH and PAS drugs independently or in combination with Aloe vera juice, no histoarchetectural changes were observed in Brain, Pancreas, Spleen, testes, and ovaries of rat. Thus, it confirms the antioxidant, anti-inflammatory and free scavenging property of Aloe vera juice to protect the histological structures of the different organs and glands of Sprague-dawley rat.
In this research work, hog plum (Spondius mangifera L.) was treated with Aloe vera gel (AVG) coating and paraffin wax-coated box (PWB) packaging and stored at ambient condition (25 ± 3 °C and 80–85% relative humidity) for 12 d to evaluate their impact on postharvest quality and storability. The physicochemical properties, microbiological analysis, and decay evaluation were analyzed throughout the storage period. The AVG and PWB coating treatments both demonstrated a significant effect on the quality of fruits during storage. The results showed that fruits with AVG coating and PWB packaging exhibited lower decay rates, weight loss, color difference, total microbial population, total soluble solids, titratable acidity, and higher fruit firmness and pH than uncoated (control) fruits. The AVG coating was the most effective treatment, followed by the PWB packaging treatment. Our findings show that the AVG coating and PWB packaging treatment can be a promising solution for preserving the quality of hog plums and also helps in increasing the lifetime of hog plums during storage.
We are continuously fighting with the pandemic of COVID-19 from last 3 years. Also other infections like Common cold, Severely Acute Viral Infections (SARS), Influenza (the flu), Herpes, Tuberculosis, Whooping cough, Pneumonia, Typhoid are some of the very common and recurring infections in human beings. Having strong immunity and destroying the main causes of various infections is the only option to survive in such conditions. It is a need to get familiar with natural herbs or dravyas from Ayurveda. Dravyas suggested in Ayurveda, which can boost immunity and thus, can protect us from various infections. In Ashtang Ayurveda, Rasayana is a special branch of Ayurveda. It promotes the inner healing power of an individual and thus can become helpful in overtaking the symptoms and diseases while promoting the immunity. Madanpal Nighantu is one of our ancient literature textbook of Ayurveda which provides information about multiple important herbal and ayurvedic drugs. In Madanpal Nighantu, 35 dravyas are explained as Rasayana dravyas in sutras. So, screening of Rasayana dravyas from Madanpal Nighantu can provide useful information to medical practitioners. These Rasayana dravyas can ultimately become useful in healthy individuals and in patients suffering from various infections or diseases in maintaining and enhancing their natural immunity. By considering all these facts, we made an effort to find out and study all these Rasayana dravyas from Madanpal Nighantu having Rasayana properties. Also this paper makes an attempt to study Rasapanchkas of these Rasayana dravyas to assess their probable mode of action as Rasayana.
Objective: Renal failure is a complication of diabetes. It can be the risk factor of cardiovascular disease. This study aimed to evaluate the effect of exercise course and Aloe-Vera supplementation on renal function and lipid profile in type 2 diabetes mellitus (T2DM) patients.
Materials and Methods: This is a clinical trial double blind with pre-test and post hoc test analyses with a control group. The studied population included a group of T2DM diagnosed patients among whom 24 men (40-60years) were selected voluntarily and purposefully and were classified into three groups by balanced block randomization method, exercise(E), exercise + supplement (E+S), and control (C), (n=8/group). Combined exercise was planned in two 12-minute repetitive sets with 70% 1RM. Then the participants ran for two 10- minute sets with 70-75% maximum heart rate intensity on treadmill. E+S participants consumed 500 mg/day Aloe Vera for 6 weeks. Obtained data by SPSS-19 software were analyzed using co-variance analysis method with Bonferroni post hoc test (P-value
Majority of the diseases occur due to decreased level of the endogenous antioxidants like SOD (Super oxide dismutase), GPX (Glutathione per oxidase) and CAT (catalyse). Therefore, exogenous anti-oxidants like vitamin C and E, zinc, beta carotene etc. need to be incorporated. These anti-oxidants protect the cell from damage caused by free radicals and increase cell longevity. In Ayurveda perspective such type of function may be recognized as Madhurarasa. Acharya Charaka mentioned a group (Skandha) of drugs basing on their rasa (taste). Also he stated that these drugs do possess not only Madhurarasa, but also Madhuravipaka and Madhuraprabhava. Scientifically one of its Rasayana properties can be evaluated by anti-oxidant activity. The present review of Madhuraskandha drugs showed that majority of drugs do possess antioxidant activity.
Background: In medicine, plants considered the main resource for drugs. In fact, it is estimated that 25% of prescription drugs have a plant origin. Aloe vera which
is a pharmaceutical plant is useful for improving the body’s physiology and could be used for curing many diseases. However, there are a few studies related to the
effects of Aloe vera on the reproductive system.
Aim of study: The main objective of this study is to investigatethe effect of perinatal and postnatal exposure to Aloe vera gel extract on the histological status of
male mice epididymis at puberty.
Materials and Method: Forty mature female Swiss Webster mice were divided intoexperimental and control groups. These female mice were mating with fertile
males.Following conception,thefemales given orally 10 μl of fresh Aloe vera gel extract started from the onset of gestationand continued throughout the gestation
period (20 days). After parturition and through weaning time these male births were given orally 10 μl of fresh Aloe vera gel extract and continuous till six weeks.
The female mice in the control group were given normal saline only by the same dose and route. Around puberty (aged six weeks) the male births were sacrificed then
an incision was made in the pelvic region to get their testes. The epididymis was grasping gently, fixed, and then histological sections with a thickness of 5 microns
were prepared.
Results: Histological observation of male mice epididymis prenatal exposure to a low dose of Aloe vera gel extract and continuous through weaning time till puberty
showed a normal structural pattern with a great number of sperms in caput (head), corpus (body) and caudal (tail) epididymis. The lumen of the cauda epididymis
contains a larger density of sperm cells.
Conclusions: Using a low dose of Aloe vera gel extract revealed a normal structural pattern of the epididymis, and it leads to enhancing the sperm amounts inside
the epididymal lumen.
Keywords: Aloe vera, Male infertility, Natural therapeutic remedies, Male reproductive system, Epididymis
Aloe vera seems to have an essential role over millennia in several cultures, as well as in Greek. Νowadays it is a fact that Greece, because of the economic crisis, focused on the cultivation of alternative plants including Aloe. This cultivation attracts the farmers' interest both for its good adaptability to the local climate and for its beneficial properties. According to the literature, they can harvest 7,000 kg of leaves containing about 70-80% gel per acre. Also, it is noteworthy that the main purpose of the cultivation of this species, is the leaves' processing in order to obtain its beneficial properties for skin, health, weight loss etc. In addition, as far as the domestic commercialization and market demand of Aloe vera are concerned, it is observed that they are still in an embryonic stage, and are in need of enrichment with direct supply chains with the pharmaceutical and cosmetic industries that will supply with Aloe's gel their production lines. The present review focuses on the current state of the Aloe's cultivation, utilization and commercialization in Greece, so it can be used as a baseline information for further research.
Aloe barbadensis leaves and Hylocereus polyrhizus are plants that are easily found in Malaysia. They arecommonly studied for antibacterial activity and how they can benefit the human being. However it is notknown about how the combination of both plants work to become the alternative way of remedies. Theobjectives of this study were to determine the antibacterial, antioxidant, and toxicity of Hylocereuspolyrhizus peels and Aloe barbadensis leaves extracts. The antibacterial activities were evaluatedtowards the bacteria commonly associated with skin infections; which are Staphylococcus aureus,Propionibacterium acnes and Pseudomonas aeruginosa, using Kirby-Bauer Disc Diffusion method. Theantioxidant activity was determined using DPPH assay. The toxicity activity was evaluated by exposingthe extracts towards brine shrimp. As for the results, Aloe barbadensis extracts showed the highestantibacterial activity compared to Hylocereus polyrhizus and the combination of both extracts. For theantioxidant activity and the toxicity test, Hylocereus polyrhizus possessed the best result, where the IC50obtained was 226.397 μg/ml and LC50 was 28588.76 μg/ml (non-toxic). In conclusion, Aloe barbadensis,Hylocereus polyrhizus and the combination of both have the potential to become alternative way asremedies instead of modern medicines. Keywords: Hylocereus polyrhizus, Aloe barbadensis, antibiotics, antioxidants, toxicity.
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