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Molecular barcoding and phylogeny analysis of Herpetogramma stultalis (Lepidoptera: Crambidae) using cytochrome oxidase subunit I gene sequence
... The identification of these two species is difficult in morphologically due to their similarities. Molecular phylogenetic analysis were extensively carried out using COI gene sequences in various group of insects like dipterans ( Bindu and Sebastian, 2014;Priya and Sebastian, 2014), lepidopterans (Akhilesh and Sebastian, 2014;Pavana and Sebastian, 2014), heteropterans (Sreejith and Sebastian, 2014), odonates (Jisha and Sebastian, 2015), and hymenopterans ( Rukhsana and Sebastian, 2014 ...
Anastatus (Hymenoptera: Eupelmidae) species are end parasitoids of a wide variety of insect eggs of Hemiptera, Lepidoptera and Orthoptera. It included the family Eupelmidae of Hymenoptera and they are an important egg parasitoid of several major insect pests. Here we report the partial sequence of cytochrome oxidase sub unit I gene (COI) of Anatatus bangalorensis (KU052674) and Anastatus acherontiae (KU052673) and its phylogenetic relationship. The COI gene sequence of A. bangalorensis and A. acherontiae are showed considerable variation with other related species. The mitochondrial COI DNA barcode developed in this study can be used for the accurate identification. The COI partial coding sequence of A. bangalorensis (KU052674) are showed 1.6% difference to A. acherontiae (KU052673), A. bangalorensis and A. acherontiae demonstrates the efficiency of the barcoding gene in discriminating global phylogeographical variants among the related species complex. A. bangalorensis and A. acherontiae are in a same clade on the phylogenetic tree.
... DNA sequences of the mitochondrial cytochrome oxidase I (COI) gene can serve as a DNA barcode for identifying all kinds of animals [3]. Phylogenetic analysis using COI gene sequences were extensively carried out by several workers in different group of organisms like southern house mosquito Culex quinquefasciatus [4], Armigeres subalbatus mosquito [5], green bottle fly Lucilia sericata [6], Herpetogramma stultalis [7], white backed plant hopper Sogatella furcifera [8], Asian honeybee Apis cerana [9] and lepidopteran species [10]. It is an important advancement in molecular biology for rapidly and costefficiently using a short reference sequence of DNA to help catalog and inventory biodiversity. ...
Lepidoptera is one of the largest orders of insects that include moths and butterflies. Most of the lepidopterans are morphologically similar, therefore the identification of these insect are tedious using morphotaxonomy and that is detrimental in designing a better strategy to control and conserve them. These are an extremely important group of 'model' organisms used to investigate many areas of biological research, including such diverse fields as navigation, pest control, embryology, mimicry, evolution, genetics, population dynamics and biodiversity conservation. Knowledge of lepidopteran genomics will create new methods of insect pest management and will contribute to sustainable agriculture, protection of the environment and the maintenance of biodiversity. In this study we have amplified cytochrome oxidase I gene of Papilio polytes for species identification and constructed phylogenetic tree for recognizing evolutionary relationship.
... The mitochondrial DNA has been extensively analysed and proven to be an important tool in species delimitation as it possesses biological properties making it suitable as a marker for molecular biodiversity. Fragment size of mitochondrial cytochrome oxidase subunit I (COI) gene has been shown to provide high resolution to identify cryptic species, thereby increasing taxonomy-based biodiversity estimates and its usefulness has been confirmed for identifying Coleoptera 4 , Diptera 5,6 , Odonata 7 , Hemiptera 8,9 , Hymenoptera 10 and Lepidoptera 11,12 . DNA barcoding has proved to be a versatile tool with a variety of applications, for example, by facilitating the association between different developmental stages in insects. ...
DNA barcoding has become the method of choice for a rapid, reliable, and cost-effective identification of pest species. Since DNA barcoding have proven to be highly efficient in identifying both immatures and adults, it is used to differentiate invasive and native pests. It has been used in managing species complexes in agricultural, IPM systems and also in the cases unpredictable species. Recently, DNA barcoding of partial mitochondrial COI gene is very popular in DNA based identification of various agricultural pest species. The present study investigates the molecular evolution of the Asota species using COI gene and its usefulness for reconstructing phylogenetic relationships within and among different Asota species.
... Phylogenetic analysis using mitochondrial COI gene sequence were extensively carried out in various insect groups like grasshopper, Microcentrum rhombifolium Ceriagrion coromandelianum 8 , cigarette beetle serricorne 9 , leaf hopper Thaia Herpetogramma stulasis 11 . ...
... Phylogenetic analysis using mitochondrial COI gene sequence were extensively carried out in various insect groups like grasshopper, Microcentrum rhombifolium Ceriagrion coromandelianum 8 , cigarette beetle serricorne 9 , leaf hopper Thaia Herpetogramma stulasis 11 . ...
... The mitochondrial DNA has been extensively analysed and proven to be an important tool in species delimitation as it possesses biological properties making it suitable as a marker for molecular biodiversity. Fragment size of mitochondrial cytochrome oxidase subunit I (COI) gene has been shown to provide high resolution to identify cryptic species, thereby increasing taxonomy-based biodiversity estimates and its usefulness has been confirmed for identifying Coleoptera 4 , Diptera 5,6 , Odonata 7 , Hemiptera 8,9 , Hymenoptera 10 and Lepidoptera 11,12 . DNA barcoding has proved to be a versatile tool with a variety of applications, for example, by facilitating the association between different developmental stages in insects. ...
For the past few years, DNA barcoding has become an efficient method for the identification of species. In this study we test the efficiency of DNA barcoding for true bug, Zicrona caerulea (Hemiptera: Heteroptera), an ecologically and economically important as well as morphologically diverse insect taxon. These bugs are useful predators of leaf beetles of the genus Altica, larvae of various beetles and caterpillars of moths, and it also feeds on plants. The study emphasizes the use of DNA barcodes for the identification of Zicrona caerulea and to represent an important step in building-up a comprehensive barcode library for the true bugs. As part of our study we analyzed DNA barcodes of Zicrona caerulea isolated from Kerala and its phylogenetic status with other related taxonomic groups. The PCR amplified cytochrome oxidase subunit I gene (COI) partial sequence of Zicrona caerulea has 1.11% difference to that of Zicrona caerulea (GenBank Accession: GQ292256) of Seoul, Korea and 1.68% difference to Zicrona caerulea (KM023126) Bremen, Germany.
Significant novelty of the study were highlighted:
• Timely identification of the new invasive pests
• Timely identification of the emerging pests
• New report of alternative host crops of the pests
• Comprehensive taxonomic database of the crop pests
• Comprehensive taxonomic database of the natural enemies
• 20 nucleotide sequences were deposited first time to GenBank nucleotide database
• Developed sequences in the study serve as reference sequence across the world
• Given the way for development of better pest management strategies.
Library for solanaceous crops pests and natural enemies along with molecular data were developed. The study added several DNA barcodes of pest and natural enemies in the existing BOLD database which serve valuable information to the researchers. Also, the whole study certainly be helpful for the scientific communities, farmers, biodiversity personnel’s and non-governmental agencies related to the agriculture sector especially in the field of crop protection.
Podagrica fuscicornis referred to as Flea beetles are members of Family Chrysomelidae (leaf beetles) and occur in all plant life habitats. Podagrica species arise most frequently inside the open, namely within the location of grasslands, forests and water bodies. Molecular characterization and DNA barcoding is a taxonomic method that makes use of a short genetic marker in an insect DNA to identify a species, which include an unknown species. DNA barcoding for species identification of the Flea beetles, P. fuscicornis isolated from Malappuram district (Kerala: India) by using the mitochondrial cytochrome oxidase subunit I (CO I) gene have been checked. DNA sequence similarity searches of COI gene of P. fuscicornis (NCBI GenBank Accession No. KX 778629) revealed that it is genetically 87% identical to Podagrica fuscipes (Accession No. KF 655901) cytochrome oxidase I gene collected from Spain. The results indicate slow evolution of the CO I sequences among the morphologically distinct and geographically isolated group of P. fuscicornis.
Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNA Cytochrome oxidase subunit I (COXI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified cytochrome oxidase I gene using Polymerase Chain Reaction (PCR) method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. A partial sequence of cytochrome oxidase I gene of Iranian goats is 1286 bp and contained four variable sites and three haplotypes. Phylogenetic analysis of haplotype in the combination with the goat from GenBank showed that Iranian goatclustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.
With millions of species and their life-stage transformations, the animal kingdom provides a challenging target for taxonomy. Recent work has suggested that a DNA-based identification system, founded on the mitochondrial gene, cytochrome c oxidase subunit 1 (COI), can aid the resolution of this diversity. While past work has validated the ability of COI sequences to diagnose species in certain taxonomic groups, the present study extends these analyses across the animal kingdom. The results indicate that sequence divergences at COI regularly enable the discrimination of closely allied species in all animal phyla except the Cnidaria. This success in species diagnosis reflects both the high rates of sequence change at COI in most animal groups and constraints on intraspecific mitochondrial DNA divergence arising, at least in part, through selective sweeps mediated via interactions with the nuclear genome.
Abstract Mitochondrial genomes provide a promising new tool for understanding deep-level insect phylogenetics, but have yet to be evaluated for their ability to resolve intraordinal relationships. We tested the utility of mitochondrial genome data for the resolution of relationships within Diptera, the insect order for which the most data are available. We sequenced an additional three genomes, from a syrphid, nemestrinid and tabanid, representing three additional dipteran clades, ‘aschiza’, non-heteroneuran muscomorpha and ‘basal brachyceran’, respectively. We assessed the influence of optimality criteria, gene inclusion/exclusion, data recoding and partitioning strategies on topology and nodal support within Diptera. Our consensus phylogeny of Diptera was largely consistent with previous phylogenetic hypotheses of the order, except that we did not recover a monophyletic Muscomorpha (Nesmestrinidae grouped with Tabanidae) or Acalyptratae (Drosophilidae grouped with Calliphoridae). The results were very robust to optimality criteria, as parsimony, likelihood and Bayesian approaches yielded very similar topologies, although nodal support varied. The addition of ribosomal and transfer RNA genes to the protein coding genes traditionally used in mitochondrial genome phylogenies improved the resolution and support, contrary to previous suggestions that these genes would evolve too quickly or prove too difficult to align to provide phylogenetic signal at deep nodes. Strategies to recode data, aimed at reducing homoplasy, resulted in a decrease in tree resolution and branch support. Bayesian analyses were highly sensitive to partitioning strategy: biologically realistic partitions into codon groups produced the best results. The implications of this study for dipteran systematics and the effective approaches to using mitochondrial genome data are discussed. Mitochondrial genomes resolve intraordinal relationships within Diptera accurately over very wide time ranges (1–200 million years ago) and genetic distances, suggesting that this may be an excellent data source for deep-level studies within other, less studied, insect orders.
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Molecular evolution of PDo 500 satellite DNA family in dolichopoda Cave Crickets (Rhaphidophoridae)
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