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Research J. Topical and Cosmetic Sci. 6(1): Jan. – June 2015
ISSN 0976- 2981 (Print)
2321-5844 (Online)
Tea Polyphenols for Skin Care
Karishma Rajbhar, Himanshu Dawda and Usha Mukundan
Plant Biotechnology Laboratory, Ramniranjan Jhunjhunwala College, Ghatkopar, Mumbai, India.
*Corresponding Author E-mail:
Dietary polyphenols of tea show antioxidant, anti-ageing, anti-wrinkle, anti-acne, anti-microbial, anti-
inflammatory and skin lightening properties which are beneficial for dermal tissue. This article explores the
potential of topical application of polyphenols from tea in skin care. The use of tea polyphenols as a topically
applied plant extract which can penetrate in the dermis has been well documented. The findings of these studies
indicate that tea polyphenols when used in an appropriate way and amount can be used to prevent skin related
KEYWORDS: Tea, polyphenols, UV radiation, skin restoration, topical application.
Polyphenols are plant secondary metabolites and are
commonly found in almost all plants. The phenolic
components of most polyphenols have a common
structural feature of an aromatic ring with at least one
hydroxyl substituent [1]. Three phenolics compounds
important for humans are phenolic acids, flavonoids and
polyphenols [2]. Phenolic acid is divided into
hydroxycinnamic and hydroxybenzoic acids [3].
Flavonoids are a group of compounds with low
molecular weight, and are derivatives of benzo-y-pyrone.
Polyphenols are compounds with high molecular weights
divided into two groups- first is condensed group made
up of polymers of catechins and epicatechins, and
second is a hydrolysable group made up of polymers of
gallic and egllagic acids [2]. Polyphenols in plants
provides chemical defence against predators and ultra-
violet radiation [3].
Received on 22.02.2015 Accepted on 05.03.2015
©A&V Publications all right reserved
Research J. Topical and Cosmetic Sci. 6(1):Jan.–June 2015 page 1-6
Tea is prepared from leaves and leaf buds of Camellia
sinensis from the family Theaceae. Types of tea like
white tea, green tea, oolong and black tea and others are
all harvested from this species, but are processed
differently to attain different levels of oxidation. The
infusion of tea leaves is a beverage which is widely
consumed second only to water [4]. The brew of one tea
bag in one cup of water produces a solution of 0.35%
w/w solids [5].
The composition of an infusion of Green tea or Black tea
is listed below [5].
Components %wt/wt Solids
Catechins 9%-30%,
Theaflavins 4%,
Simple polyphenols 2%- 3%,
Flavonols 1%-2%
Other polyphenols 6% -23%,
Theanine 3%
Amino acids 3%
Peptides and protein 6%
Organic acids 2%,
Sugars 7%
Other carbohydrates 4%
Lipids 3%
Caffeine 3%,
Other methyl xanthines <1%
Potassium 5%
Other minerals and ash 5%
Trace of aroma -
Research J. Topical and Cosmetic Sci. 6(1): Jan. – June 2015
On the basis of the extent of enzymatic reactions which
occur during the manufacture, tea is classified into the
following types: green tea (non-fermented), black tea
(fermented) and oolong tea (specially treated and semi
fermented). The oxidized polyphenols in black tea are
responsible for briskness, strength, colour, taste and
astringency of the black tea infusion [6].
The polyphenols found in tea are more commonly
known as flavanols or catechins like epicatechin,
epicatechin-3-gallate, epigallocatechin, and
epigallocatechin-3-gallate (EGCG), with the latter being
the highest in concentration [7]. The principal
constituents are caffeine, tannins and essential oils.
Tannins comprise of a variety of polyphenolic
compounds - most important of these are flavonoids
called catechins [6]. Green tea contains higher amounts
of catechin derivatives, such as (-)-epicatechin (EC), (-)-
epigallocatechin (EGC) and their gallates (ECG and
EGCG). During the production of black tea, some of the
catechins are converted to theaflavins (TF) and
thearubigins (TR) by enzymatic oxidation and coupling
reactions (Roy; 2001). Catechins and their derivatives
are known to contribute towards the flavour of tea while
the aroma is dependent on the presence of different
volatile compounds. Theaflavins (TF) are responsible for
briskness and brightness while thearubigins (TR) for
colour and body or strength (mouth feel). Caffeine is
responsible for the stimulating effect of tea and the
cancer chemopreventive action of tea is mainly due to its
polyphenolic content [6].
The part of electromagnetic spectrum of the wavelength
between 200nm to 400nm is ultra-violet (UV) radiation.
It is divided into long wave UVA (320nm-400nm),
medium wave UVB (280nm-320nm) and short wave
UVC (200nm-280nm) [8]. The longer wave is called
aging ray which penetrates deep in the epidermis and
dermis of skin. UVA is thousand times effective in
tanning effect than UVB. Tanning is caused by melanin
darkening in epidermis. Long term UVA exposure burns
sensitive skin and if last prolonged than can damage
inner structure of corium and results in premature photo-
aging of the skin. UVA induces endogenous
photosensitization responses in cells. Necrosis of
endothelial cells is caused by UVA injury and leads to
dermal blood vessel damage. UVA injury damage
structural DNA. Impair immune system and causes 67%
of malignant melanoma [9].
UVB radiation is called burning ray and makes up 4.5%
of UV light. UVB is less but most active in solar light,
UVB is thousand times stronger than UVA causing
sunburn. UVB is more genotoxic than UVA. UVB acts
in epidermal basal cell of skin; it’s directly or indirectly
induces adverse biological effects, like formation of
pyrimidine, photoproducts, isomerisation of trans to cis
urocanic acid, induction of ornithine decarboxylase
activity, stimulation of DNA synthesis, free radical
production in the skin, cell cycle growth arrest,
photoaging and photocarcinogenesis.
For showing biological effects UV must be absorbed by
cellular chromophore which transforms the energy into
biochemical signals. Nucleic acid and proteins are major
cellular chromophores absorbing radiation in the UVB
wavelength range. A DNA lesion is seen if DNA absorbs
UVB. Lesions like cyclobutane-pyrimidine dimers and
pyrimidine-pyrimidine photoproducts. DNA
photoproduct formation leads to the activation of p53
protein that induces apoptosis of irradiated keratinocytes
by blocking RNA transcription. Damage occurring in S-
phase of cell cycle might lead to mutagenesis resulting in
mainly cytosine to thymine substitution. On the other
hand more UV exposure results keratinocytes to lose
their ability to undergo the apoptotic process. Amino
acids like tryptophan, tyrosine and biomolecules like
NADH, quoins, flavins, porphyrins, 7-
dehydrocholesterol and urocanic acid absorbs UVB
radiations [9, 10].
Skin damage can be caused by UV radiation even if
exposed for 2 hours. Keratinosomes decrease is an
indicator of skin damage which forms dyskeratotic cells,
16 hours to 18 hours exposure results in intracellular
edema whereas 30 hours to 48 hours intercellular edema
which develops around damaged keratinocytes.
Degeneration of keratinocytes cause mitochondrial
swelling and rupture, cytoplasm condensation and
pyknotic nuclei appears which is caused by 48 hours to
72 hours of total UV range exposure. This also results
into Reactive Oxygen Species (ROS) largely seen in
keratinocytes and fibroblasts and is taken out by non-
enzymatic enzymatic antioxidants. ROS active
metabolites like hydroxyl radical, superoxide active
anion and peroxyl radicals and most active ones are
single oxygen, hydrogen peroxide and ozone. Reactive
Nitrogen Species (RNS) are nitric oxide and nitric
dioxide. Free radicals modulate progressive deterioration
of cellular structure function resulting loss of cellular
integrity by DNA modification and abnormal expression
of cellular genes [10].
UV radiation cause skin pigmentation on exposure,
melanocytes increase the production of intracellular
nitric oxide, triggering the signal transduction cascades
to initiate melanogenesis by tyrosinase. Melanogenesis is
influenced through a paracrine regulation process
involving the keratinocytes [11].
Research J. Topical and Cosmetic Sci. 6(1): Jan. – June 2015
Antioxidant activity of herbal phenolic acids and
flavonoids such as quercetin, luteolin and catechins are
better antioxidants than the nutrients vitamin C, vitamin
E and β-carotene [2, 5]. Phenolics may be beneficial in
preventing UV-induced oxygen free radical generation
and lipid peroxidation, i.e. events involved in
pathological states such as photoaging and skin cancer.
This review is a summary of the findings of the pho-
toprotective effects of tea polyphenols in studies of the
skin damage induced by UV irradiation [12].
Flavonoids are antioxidant molecules. They absorb UV
light and modulate signalling pathways which influences
cellular function which can be beneficial for skin health.
UV radiation shows negative effects on skin like
erythema, edema, sunburned cells, hyperplasia,
inflammation, immune-suppression, photoaging and
photocarcinogenesis. Certain flavonoids can minimize
the adverse skin reactions, demonstration on cell culture,
animals, and humans have been proved. Green tea
polyphenols shows immense effect when used as
chemoprevention and it is most potent at suppressing the
carcinogenic activity of UV radiation. Green tea
polyphenols are photoprotective on cellular, molecular
and biochemical mechanisms in both in vitro and in vivo
systems [8, 10].
Polyphenols have been extensively studied as potential
chemopreventive agents that could act against external
inflammatory stimuli including tumour promoting agents
and solar ultraviolet (UV) radiation. Catechin,
epicatechin, epigallocatechin, epicatechin gallate and
epigallocatechin gallate, are effective free radical
scavengers, chain-breaking antioxidants and scavengers
of reactive nitrogen species. The tea polyphenols have
been attributed both antioxidant properties as scavengers
of reactive oxygen species and the activation of phase II
detoxifying enzymes [12].
Epigallocatechin (EGC) and epigallocatechin gallate
(EGCG) are the predominant catechins in tea protect
against UVB-induced skin carcinogenesis in mice
projected that reactive oxygen species generated by
UVB play a fundamental role in the process of
carcinogenesis and that tea polyphenols acted by
quenching such oxidant species . UVA component of
solar radiation exerts its biological effects primarily by
oxidative and antioxidants like the water-soluble
ascorbic acid, and the lipophilic K-tocopherol and
butylated hydroxytoluene are believed to act as photo-
protective agents by their ability to scavenge reactive
oxygen species generated during UVA irradiation. Tea
polyphenols may have a pro-oxidant role leading to the
generation of hydrogen peroxide in human cancer cell
lines, regulation of UVA-activated stress response genes
in human skin cells. EGC can modulate the expression of
haem oxygenase-1 (HO-1), cyclooxygenase-2 (Cox-2)
and metalloproteinase-1 (MMP-1) in UVA-irradiated
dermal and transformed epidermal keratinocytes [12].
Tea polyphenols are powerful antioxidants and
anticarcinogenic compounds, specifically the catechins
epigallocatechin-3-gallate (EGCG), epigallocatechin
(EGC), and epicatechin-3-gallate (ECG), which account
for 30-40 percent of the extractable solids of tea leaves,
are believed to mediate many of the cancer
chemopreventive effects. Mechanisms of action may
include antioxidant and free-radical scavenging activity,
and stimulation of detoxification systems through
selective induction or modification of phase I and phase
II metabolic enzymes. Tea inhibits biochemical markers
of tumour initiation and promotion, including the rate of
cell replication and thus inhibition of the growth and
development of neoplasms. Tea may serve to protect
against skin cancer. Tea consumption inhibited the
formation of tumours associated with the exposure to
UVB sunrays. Where UVB tumours already existed, tea
drinking slowed their growth and in some cases, actually
decreased their size [3, 12].
EGCG reactivated dying skin cells when exposed during
growth of the cells. The skin consists of three layers: the
epidermis (outer layer), dermis (middle layer) and
hypodermis (inner layer). Tea polyphenols are not
absorbed beyond the epidermis, so some benefits are
restricted to that outer layer of skin. But EGCG may be a
fountain of youth for skin cells as when exposed to
EGCG; the old cells found in the upper layers of the
epidermis appear to start dividing again as they make
DNA and produce more energy and the cells reactivated.
EGCG accelerates the differentiation process among new
cells. These two effects of EGCG on skin cells in
different layers of the epidermis could be potential
benefits for skin as diverse as aphthous ulcers, psoriasis,
rosacea, wrinkles and wounds. Possibly scar tissue could
be prevented from forming with EGCG therapy and
diabetics patients with slow healing wounds may benefit
from EGCG supplementation [12].
Caffeic acid (3,4-dihydroxycinnamic acid) and ferulic
acid (4-hydroxy-3-methoxycinnamic acid) have been
demonstrated to protect phospholipidic membranes from
UV-induced peroxidation by inhibiting propagation of
the lipid peroxidative chain reaction and to react with
nitrogen oxides and proved effective in protecting
human skin from UVB-induced erythema. Ferulic acid,
shown to be a strong UV absorber, is employed as a
photoprotective agent in a number of skin lotions and
sunscreens [3].
Research J. Topical and Cosmetic Sci. 6(1): Jan. – June 2015
Quercetin (3, 5, 7, 3’, 4’-pentahydroxyflavon) a
powerful antioxidant and metal ion chelator, is believed
capable of preventing the harmful effects of UV light or
at least of reducing the damage. Quercetin protected skin
antioxidant systems, namely glutathione peroxidase,
glutathione reductase, catalase and superoxide dismutase
activities, against UVA irradiating damage in rats to a
considerable degree. Oral intake of quercetin prevented
UVB-induced immune suppression in SKH-1 hairless
mice. In vitro, quercetin and its semi-synthetic
derivatives (quercetin 3-O-acetate, quercetin 3-O-
propionate, and quercetin 3-O-palmitate) were found to
inhibit UVC radiation-induced peroxidation in liposomal
membranes [3].
Photoprotective effects of green tea polyphenols,
catechins and others have been demonstrated on humans
by both oral supplementation and topical application.
Plant extracts is dissolved in solvent so that only plant
extracts remains on the dermal tissues. Although
delivery is an issue as they cannot penetrate and function
on human skin as the market commercial product do.
Large influence is exerting by flavonoids because of
their specific and nonspecific affinity for different types
of proteins throughout the cell. Flavonoids physically
block UV penetration, influence DNA repair, attenuate
the inflammatory response, preserve immune function
and induce cytoprotective pathways have been proved.
Mechanisms by which flavonoids protect skin from
harmful effects of UV radiation are still under
investigation. Topical application of EGCG may prevent
UV-B induced immune suppression and precancerous
cell changes after UVB exposure. Anti-inflammatory
and anticancer property of EGCG and green tea
polyphenols can be used to control onset and growth of
skin tumours [8, 9].
Antioxidant properties of tea show beneficial effect of
tea. Green and white tea shows protection against
detrimental effects of UV on cutaneous immunity. UV is
not absorbed by topical applied tea products or sunscreen
as both have presence of sun protection factor of one.
Green and white teas are potential photoprotective agent
which can be used in conjunction for sun protection
methods. Skin carcinogenesis is result of UV irradiation,
thus green tea extract can be used. Green tea extract
show reduction of UV induced erythema, DNA damage,
formation of radical oxygen species and down regulation
of numerous factors related to apoptosis, inflammation,
differentiation and carcinogenesis. Green tea extract s
chemicals stability and staining properties are unstable.
High concentration limits the usability of high green tea
extracts in cosmetic products. Low concentration green
extracts can be photochemopreventive to skin if doses
are taken regularly. Topical green tea extracts also
reduces UVB mediated epithelial damages [13].
1. Antioxidant properties
EGCG is at least 100 times more effective than vitamin
C and 25 times better than vitamin E at protecting cells
and their genetic material DNA from damage, believed
to be linked to cancer, heart disease and other fatal
illness. EGCG carries twice the antioxidant potential of
resveratrol. It induces inhibition of soyabean
lipoxygenase, inhibits TPA and induces oxidative DNA
base modification in HeLa cells. It also inhibits Cu2+
mediated oxidation of low density lipoprotein (LDL) and
reduces tertiary butyl hydro peroxide which creates lipid
peroxidation and blocks the production of reactive
oxygen species derived from NADPH-cytochorme P450-
mediated oxidation of the cooked meat carcinogen i.e 2-
amino-3- methylimidazole (4-5-f) quinoline. Green tea
water soluble therefore excessive amounts of
antioxidants are excreted out by body. Antioxidant
activity of EGCG helps tremendously to combat post
exercise muscle soreness [8].
2. Anti-ageing and anti-wrinkle properties
Free radicals promote oxidation of nucleic acids,
proteins, and lipids and can damage intracellular
structures including DNA by regulating transcription
factors, such as activator protein 1 (AP-1) and nuclear
transcription factor-kappa B (NF-kB). Polyphenols
inhibit collagenase activity by two mechanisms first by
binding of the essential Zn2+ in the enzyme active site
and second by conformational changes on the enzyme
due the ability of phenolic compounds to interact with
proteins. Metalloproteinases are produced by AP-1
which breaks existing collagen, contributing to skin
wrinkling. It is the sum of NF-kB transcription of
proinflammatory mediators interleukin (IL)-1, IL-6, IL-
8, and tumor necrosis factor-alpha acting through the cell
surface responsible for skin aging. Tea polyphenols and
EGCG in addition are effective free-radical scavengers,
down-regulate UV-induced expression of AP-1 and NF-
kB and suppress metalloproteinase and age-related
collagen cross-linking in mice. Tea polyphenols inhibit
the activity of collagenase and increases collagen
biosynthesis rate of human fibroblasts. EGCG topical
treatments influence HIF-1α induction and VEGF
(vascular endothelial growth factor) expression and may
serve as a potential agent in the prevention of
telangiectasia (spider veins) [11, 14].
Research J. Topical and Cosmetic Sci. 6(1): Jan. – June 2015
3. Anti-acne properties
Acne vulgaris is the most common skin condition
resulting from the effects of hormones. Androgens such
as dihydrotestosterone (DHT) and testosterone, the
adrenal precursor dehydroepiandrosterone sulfate
(DHEAS), estrogens such as estradiol, and other
hormones, including growth hormone and insulin-like
growth factors (IGFs), could be important in acne. It is
being studied whether hormones are taken by serum of
sebaceous gland or produced locally within the gland or
whether a combination of these processes is involved.
Hormonal therapy is an option in women with acne not
responding to conventional treatment or with signs of
endocrine abnormalities. Study depict potential effects of
stable formulation (water in oil emulsion), containing
3% tea extract on skin sebum production for a period of
8 weeks. A statistically significant (p < 0.5%) decrease
was found in skin sebum production after long term
application of the formulation [15]. EGCG inhibits
lipogenesis. Epigallocatechin-3-gallate (EGCG)
decreases inflammation induced by SEB-1 sebocytes
stimulated by heat-inactivated Propionibacterium acnes
through the inhibition of NF-jB and activator protein 1
(AP-1) pathways. Biochemical, genetic, and cellular
studies indicate that modulation of AMPK–SREBP-1
and NF-kB/ activator protein 1 (AP-1) signalling
pathways mediates the sebosuppressive and anti-
inflammatory effects of EGCG [16].
4. Skin lightening properties
Natural skin lightening products containing tyrosinase
blockers like phenols and polyphenols, and non-
tyrosinase blockers like α- MSH, melanosome
transferase and cytokine inhibitors. Gallic acid
derivatives of hydroxyflavanols had been isolated from
tea as they are identified as strong tyrosinase inhibitors.
EGCG and hydroxyflavanols are not only tyrosinase
inhibitors, but also decreased MITF (Microphthalmia-
associated transcription factor) production in cells.
Ellagic acids (EA) polyphenol found in tea with strong
antioxidative properties and tyrosinase inhibition.
Chelating copper at the active site of tyrosinase to reduce
its activity and inhibition of proliferation of melanocytes
and melanin synthesis results in skin lightening by EA.
EA also show antioxidative and ROS-scavenging
activities which contribute to its skin lightening effect [9,
5. Anti-viral properties
Microbial agents, viruses or bacteria attack skin and
cause local inflammation. Inflammation can be due to
internal elements such as autoimmune diseases that are
associated with damage to the skin, resulting in skin
lesions, rash, and altered appearance. Tea polyphenols
have been recognized for prevention of infection by a
range of viruses, such as adenovirus, Epstein-Barr virus
and influenza virus. Studies shows that EGCG
inactivated HSV (Herpes simplex virus) projecting that
the antiviral properties of polyphenols are due to their
influential protein binding capacity, follow-on tight
binding to the viral coat proteins, and their ability to
transform the dynamics of the cell plasma membrane,
preventing the entry of viral particles into the cells [8,
6. Anti-Inflammatory properties
The conversion of arachidonic acid into various
proinflammatory agents, including leukotrienes is
catalyzed by the dioxygenase enzyme. Epicatechin
inhibits the dioxygenase and LTA 2 synthase activities
of 5-LOX. Epicatechin has also been shown to inhibit
15-LOX-1 [17].
Tea is generally considered a safe, non-toxic beverage
and its consumption is usually without side effects. The
average cup of tea contains from 10-50 mg of caffeine,
and over-consumption may cause irritability, insomnia,
nervousness, and tachycardia. Because studies on its
possible teratogenic effect are inconclusive, caffeine
consumption is contraindicated during pregnancy.
Lactating women should also limit caffeine intake to
avoid sleep disorders in infants [7].
Human skin is constantly exposed to the UV irradiation
present in sunlight which induces a number of
pathobiological cellular changes. Tea polyphenol
becomes one of the favourite ingredients for cosmetic
preparations as although the effects may be small, they
are significant and do meaningfully improve skin feel
and appearance with continued use. Also the component
are beneficial by being stable in production, storage, and
use, be nontoxic to the consumer and have activity at the
target site once applied. The development of novel
defensive and beneficial strategies can be studied by
molecular mechanism of UV-damage and plant
phenolics effects for prevention of the unpleasant effects
of UV radiation on the skin and improving skin
penetration of this bioactive cosmetic by enhancing
delivery into the skin.
The authors would like to thank HUL (Hindustan
Unilever) for financial assistance for the collaborated
Research Project and the fellowship provided to the first
Research J. Topical and Cosmetic Sci. 6(1): Jan. – June 2015
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... 14 The ability of polyphenols as photoprotectors are important in their use in cosmetic products. The sun protection factor (SPF) from flavonoids, stylbene and hydroxycinamic acid derivatives, is determined from 7 to 29, based on minimal SPF values (SPF 2-12) and moderate (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). In addition, the ability to prevent or reduce photodamages due to UV makes these natural polyphenols as a relevant topical ingredient. ...
... 19 The high polyphenol content in green tea can also protect the skin from the premature aging which mainly caused by UV exposured, through its function as anti-melanogenic, anti-wrinkle, antioxidant, anti-inflammatory effects and prevents immunosuppression. 20 Widiyowati H (Denpasar, 2017) conducted an in vitro study, showed that the used of of cream containing green tea extract that 70% could prevent the reduction in the amount of collagen dermis and increase Matrix metalloproteinase-1 (MMP-1) expression in Balb/C mice that was given ultraviolet B exposured. 21 Another study, performed on 42 Korean women by applying green tea extract to wrinkles on the corner of the outer eye (crow's feet), twice daily for 8 consecutive days, showed that green tea extract can inhibit free radical activity and has an antiwrinkle effect. ...
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Skin aging is a complex biological process influenced by a combination of intrinsic and extrinsic factors, leading to cumulative alterations of skin struture, function and appearance. Polyphenols represent a superfamily of diverse naturally occurring phytochemicals. Current research reveals that phenolic compounds in plants possess high antioxidant activity and free radical scavenging capacity and can prevent the body from oxidative damage over human life span. This review focuses on present understanding of skin aging and the effects of polyphenols in the context of anti-agieng activity.
... Đồng thời khả năng kháng khuẩn của catechin cũng phụ thuộc vào nồng độ catechin sử dụng trong quá trình ức chế và diệt khuẩn, kết quả này tƣơng thích với kết luận của Reygaert và đồng tác giả năm 2018 [12]. Theo nghiên cứu của Rajbhar và đồng tác giả năm 2015, catechin đƣợc chiết xuất từ trà xanh (Camellia sinensis) đƣợc dùng bôi lên da giúp ngăn chặn các chủng vi sinh có hại xâm nhập vào lớp hạ bì của da gây viêm nhiễm, bên cạnh đó catechin đƣợc xem là một nguyên liệu mỹ phẩm đa năng có tác dụng kháng oxy hoá, chống lão hoá, kháng khuẩn, kháng viêm,… [15] Điều đó cho thấy hợp chất tách chiết trong nghiên cứu của chúng tôi tuy ở dạng hợp chất dẫn xuất catechin nhƣng cho hiệu quả vƣợt trội với chuẩn đối chứng (Vitamin C) trong thí nghiệm khảo sát khả năng kháng oxy hoá và kháng khuẩn phổ rộng nhƣ các nghiên cứu trƣớc đây, phù hợp sử dụng làm nguyên liệu mỹ phẩm mang tính năng trị liệu cho làn da. ...
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Catechin chiết xuất từ lá trà xanh được biết đến với nhiều hoạt tính sinh học cao và được ứng dụng nhiều trong các lĩnh vực, đặc biệt là mỹ phẩm. Nghiên cứu được thực hiện nhằm đánh giá khả năng bắt gốc tự do và khả năng ức chế vi khuẩn gây bệnh trên da từ catechin. Kết quả cho thấy hiệu quả bắt gốc tự do rất tốt với giá trị EC 50 đạt 4,963 ug/mL. Catechin có khả năng đối kháng với các chủng vi khuẩn P. aeruginosa, S. typhi và nấm C. albicans, với giá trị MIC đạt từ 3,125-12,5 mg/mL và khả năng diệt khuẩn đạt 99,9% ở các nồng độ diệt khuẩn từ 12,5-50 mg/mL trên 3 chủng khảo sát. Điều này có thể thấy rằng việc ứng dụng catechin từ lá trà xanh làm nguyên liệu mỹ phẩm để giải quyết các vấn đề về lão hóa da và ức chế vi sinh vật gây bệnh trên da là hoàn toàn có cơ sở.
... Tea and its extracts have historically been used as essential ingredients in cosmetic manufacturing. Many benefits are available, owing to their functional components such as flavonoids, amino acids, caffeine, theaflavins, and catechins [2,35,37]. These substances, however, can not readily be absorbed or immediately used by the human body. ...
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Biotechnology, cosmetics, and aesthetic remedies are now inextricably intertwined due to the production of alternative, more effective, and safer active ingredients. Additionally, there has been an increase in demand for natural cosmetic ingredients across the globe. Camellia sinensis var. assamica (Miang tea) is a good alternative because of several biological activities, and is commercially cultivated as a resource in northern Thailand. The process of fermentation mediated by probiotics can enhance the bioavailability of compounds, transform bioactive compounds, and decrease chemical solvent use for sustainability. This study aims to apply the functional evaluation of Miang tea bio-extracts to promote skin health. On the basis of their bioactive enzymes, β-glucosidase, and antioxidant properties, the strains Lacticaseibacillus rhamnosus (previously Lactobacillus rhamnosus), Lactiplantibacillus plantarum (previously Lactobacillus plantarum), and Saccharomyces cerevisiae were used as mixed probiotic starter cultures. The activities of white, green, and black Miang tea bio-extracts, including ferric reducing antioxidant power, lipid peroxidation, nitric oxide inhibition, tyrosinase inhibition, collagenase inhibition (MMP-1 and MMP-2), and antimicrobial activity, were all considerable after 7 days of fermentation time. Additionally, phenolic antioxidant compounds (gallic acid, epigallocatechin gallate, caffeic acid, caffeine, and p-coumaric acid) were identified. The current study’s findings can determine the most effective fermentation time and dose of bio-extract, as well as suggest improvements in bioactive compounds for use in skin care formulations. These results will be used for testing on human participants in further work.
... In vitro and in vivo clinical studies on animals and humans suggest that green tea polyphenols are photoprotective in nature and can be used as pharmacological agents to prevent UVinduced skin disorders, including photoaging, melanoma, and nonmelanoma human skin cancer. EGCG is at least 100 times more effective than vitamin C and 25 times better than vitamin E in protecting the cells and their genetic material [19]. The Journal of the American Medical Association of Dermatology has noticed that "green teas have anti-inflammatory and anti-cancer potential, which can be used against various skin disorders" [20]. ...
The latest trend in textile industry promotes products with added value that provide additional comfort to users and have a focus on health in terms of use. In that sense, biofunctional and intelligent textile products with different types of applications for improving the lifestyle of the modern consumer stand out. Cosmetic textile is a high-performance textile which represents a fusion of textile material with cosmetics. The main challenges in the manufacture of such products are the selection of products with a cosmetic effect for a particular purpose, storage of agents in the structure of the textile, the rate of release of the agent on the skin and the stability of the agent to the maintenance procedures of textiles and clothing. This paper provides an overview of cosmetic agents for application on textiles, methods of their storage and release and the techniques applicable on textile. Finally, a range of commercially available cosmetic textile products is presented.
The primary goal of this research was to develop and test a herbal sunscreen cream containing Hibiscus (Hibiscus rosasinensis) and Rose flower extracts (Rosaceae). Hibiscus and Rose extractswere used in the formulation of oil in water (O/W) emulsion-based cream. Flower extracts were preparedusing the Soxhlet apparatus. The physicochemical parameters of the formulated herbal sunscreen creams were tested, including colour, odour, spreadability, pH, and viscosity.The centrifugation process was used to conduct the stability and sensitivity tests. A UV Spectrophotometric method of Mansur et al. was used to calculate the in-vitro SPF of the formulations. The flower extract-based sunscreen cream provided high absorbance in the 290-320nm wavelength range, with an SPF of 38.12-42.24. The ProShine MBTMGMIHS herbal formulation was found to be stable, spreadable, homogeneous, non-greasy, and displayed no signs of phase separation. The pH of the cream was found to between 5.5-6 which is nearer to the skin's pH.Also, after the microbial evaluation of ProShine MBTMGMIHS herbal sunscreen, no bacterial or fungal contamination was observed after incubation for a long period.We may assume from the study's findings that the formulated sunscreen can greatly improve and add to the UV absorption properties of conventional sunscreen formulations. It would also aid in the broadening of the sunscreen's UV defence performance, with the added benefit of preventing the negative and undesirable effects of synthetic sunscreen compounds.
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Herbata chińska charakteryzuje się wysoką zawartością składników farmakologicznie i kosmetycznie czynnych (kofeina i szereg związków polifenoliwych). Stanowi składnik wielu nawilżających preparatów kosmetycznych. Celem badania była ocena wpływu maski do rąk z wodno-glicerynowym ekstraktem z Camelia sinesis (L.) O. Kuntze na wybrane cechy skóry. Z grupy 24 wolontariuszek (20,2 ±0,53 lat) wyłoniono grupę badaną (kosmetyk z ekstraktem roślinnym, n = 8), porównawczą (kosmetyk placebo, n = 8) i kontrolną (brak stosowania kosmetyku, n = 8). Łącznie badanie ukończyły 22 osoby. Badanie ankietowe dotyczące subiektywnej oceny jakości skóry dłoni oraz pomiary cech skóry z zastosowaniem specjalistycznych sond (korneometr, pHmetr, tewametr, identometr) wykonano przed oraz po 3 dniach stosowania kosmetyku. Jedynie dla grupy placebo wykazano znamienne różnice poziomu nawilżenia (p = 0,005). Pokazuje to znaczenie bazy kosmetyku jako składnika wpływającego na nawilżenie. Dodatek ekstraktu niwelował ten efekt. Inne badane cechy skóry nie uległy zmianie. Oceniając wyniki oceny subiektywnej i obiektywnej poziomu nawilżenia skóry, uwidoczniono dodatnią korelację pomiędzy jej wynikami. Pozostałe cechy (pH, TEWL, elastyczność skóry) nie uwidoczniły znamiennych korelacji. Ekstrakt z zielonej herbaty wykorzystany w prostej formie kosmetycznej nie jest składnikiem poprawiającym nawilżenie. Jego dodatek do kosmetyku zamaskował wręcz działanie nawilżające składników bazy kosmetycznej, za co odpowiadają najprawdopodobniej związki garbnikowe obecne w surowcu roślinnym. W krótkotrwałej obserwacji nie wpływa także na jej odczyn kwasowo-zasadowy, funkcje barierowe skóry i jej sztywność. W trakcie naboru ani w trakcie przeprowadzania badań żadna z uczestniczek nie miała żadnych działań niepożądanych wynikających ze stosowania badanego preparatu kosmetycznego.
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Introduction. Globally, the consumption and production of tea are on the rise because of its beneficial constituents. Scarce literature exists on the effects of extraction periods on the contents of the biologically important and protective phytochemicals such as phenolics, flavonoids, and antioxidants in locally produced teas in Uganda. Aim. This study determined the effects of extraction periods on the aqueous total phenolic content (TPC) of local Camella sinensis, black primary grades and green tea, and their ecological differences, their total flavonoid content (TFC), and antioxidant capacities (AOC). Methods. Samples of local tea were collected from Kigezi, Ankole, and Buganda regions, and those of green tea were purchased from a local supermarket in Uganda. Four- and 40-minute infusions were separately prepared for each sample. Total phenolic and flavonoid contents were determined using the Folin–Ciocalteu and aluminium chloride methods using garlic acid and quercetin as standards, respectively. Antioxidant content was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing assay power (FRAP) methods, using ascorbic acid as the standard. Results. Green tea had the highest total phenolic content both with four-minute (9.50 ± 0.25 mgGAE/g) and 40-minute (25.81 ± 1.13 mgGAE/g) extractions, followed by D1 (4.14 ± 0.33 mgGAE/g) at four minutes and PF (23.60 ± 2.37 mgGAE/g) at 40 minutes. Regionally, Kigezi (4.71 ± 0.09 and 22.13 ± 0.85 mgGAE/g) at four and 40 minutes, respectively, gave the highest TPC. In TFC, tea from Buganda (4,371 ± 0.00 μgQE/g) was the highest. In DPPH and FRAP, GT (93.82 ± 0.03%, 39.04 ± 0.02 AAEμg/mL) was the best, followed by Buganda tea (88.71 ± 0.03%, 36.99 ± 0.01 AAEµg/mL), respectively. Conclusion. Longer extraction periods increase TPC in all teas. Green tea generates approximately twice the TPC generated by black tea in four-minute infusions. Green tea gives higher TPC, DPPH, and FRAP but less TFC than some black teas and is perhaps the best in terms of protection against oxidative damage to the body.
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Large amounts of solid wastes such as spent coffee grounds (SCGs) from brewing provide a valuable sugar source to investigate. The effects on the sugar properties of extraction factors were studied. Different solvent extractions using an autoclave showed distinguishable sugar contents and properties. Water extracted the highest total sugar content while alkali extracted the highest total phenolic content (TPC). The ultrasonic-water-bath-assisted extraction with water did not produce any significant content or TPC. Finally, the combination of ultrasonic-autoclave-assisted extraction with water at 40% amplitude for 10 min produced the highest total sugar content and TPC, similar to that found in samples from the autoclave extraction with water. The FT-IR spectra of SCGs sugar revealed both amorphous and crystalline structures. All sugar extracts from SCGs contained phosphorus, potassium and calcium as the main mineral elements. Thus, sugar extracts from SCGs can be considered as an alternative additive with a good TPC for food products.
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Perilla is a rich source of polyphenols, which exhibits antioxidant, anti-inflammatory activities, and a variety of biological effects. The effect of differential solvents on the polyphenols, flavonoids, rosmarinic acid (RA), antiinflammatory and antioxidant activities of perilla leaf require investigation. In this study, perilla leaf was extracted with 70% ethanol and sequentially fractionated according to the solvent’s polarity with hexane, dichloromethane, ethyl acetate, and water. Samples were subjected to the bioactive compound measurements. The antioxidant and antiinflammation nature of perilla was analyzed based on the scavenging effects on DPPH•, ABTS•+, O2•- and nitric oxide (NO), as well as FRAP assay, and determination of the inhibition effects on NO, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) production in the cell-based study. The results indicate that among all different solvents used for sequential fractionation, ethyl acetate (EtOAc) was most effective in the separation of anti-oxidative and antiinflammatory compounds in the perilla leaf extract. These properties can partly be due to the presence of polyphenols, flavonoids, and also RA. It can be demonstrated here that, the perilla leaf EtOAc fraction could be used as a natural active pharmaceutical ingredient for dietary supplements and nutraceuticals.
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Hand hygiene is now creating more awareness in the people due to pandemic COVID -19. It plays important role in the prevention, control and reduction of any acquired infection. This can stop the chain of transmission of microorganism and other bacteria from hand to different parts of our body. Herbal medicines have been extensively utilized as effectual remedies for the prevention and management of multiple health conditions. The present research was carried out to formulate and evaluate the poly herbal hand sanitizer using extract and Clove oil. The formulation was evaluated for its physical parameters. It is sure that the combination of ingredients behaves as an effective hand sanitizer.
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Safflower is called as the 'beneficial flower' because 'it helps human health', and it was introduced as red flower in Tonguibogam due to the red color of floral leaf. From old times, it has been used for the material of cloth and rouge. Recently, polyphenol compound, the main ingredient of safflower, known as anti-aging and anti-oxidizing material in the healthy food industry becomes the emerging hot topic. This study aims to confirm by DDT (Disk Diffusion Test) assay, MTT assay, and NF-kB Luciferase activity inhibition assay in vitro that polyphenol compound, which is the main ingredient of safflower, has the anti-microbial efficacy to inhibit the growth of acne germs that make troubles for the teenagers or middle aged. Also it aims to evaluate its clinical efficacy on the acne skin, utilizing the facial cleansing cosmetic form of soap sample. This study can contribute to take a major step forward to the development of cosmetic soap for acne in the cosmeceutical industry.
In response to the increased popularity and greater demand for medicinal plants, a number of conservation groups are recommending that wild medicinal plants be brought into cultivation. Green Tea is one of the most ancient and popular therapeutic beverages consumed around the world. This product is made from the leaf of the plant called "Camellia sinensis". It can be prepared as a drink, which can have many systemic health effects or an "extract" can be made from the leaves to use as medicine. Green tea is reported to contain thousands of bioactive ingredients which are almost contributed by polyphenols which plays a key role in prevention and treatment of many diseases. The aim of this literature review was to illustrate therapeutic properties of the plant "Green tea".
The research and development of cosmeceuticals is booming in recent years. Many substances, from botanical are tested or investigated as the active ingredients in cosmeceuticals. Green tea polyphenols have gained high popularity in cosmetic arena for their skin improving property. This review is an attempt to collect the scientific data of green tea polyphenols on major cosmetic problems like aging, wrinkle, photo-damage, skin darkness, acne, dandruff and hair loss. This communication covers green tea effect, their mechanism of action and clinical trials conducted on humans for different cosmetological conditions.
Acne vulgaris is a highly prevalent skin disorder characterized by hyperseborrhea, inflammation, and Propionibacterium acnes overgrowth. Only isotretinoin and hormonal therapy reduce sebum production. To identify a new drug candidate that modulates sebum, we examined the effects of EGCG, the major polyphenol in green tea, on human SEB-1 sebocytes and in patients with acne. In SEB-1 sebocytes, we found that EGCG reduced sebum by modulating the AMPK-SREBP-1 signaling pathway. EGCG also reduces inflammation by suppressing the NF-κB and AP-1 pathways. EGCG also induces cytotoxicity of SEB-1 sebocytes via apoptosis and decreases the viability of P. acnes, thus targeting almost all the pathogenic features of acne. Finally, and most importantly, EGCG significantly improved acne in an 8-week randomized, split-face, clinical trial, and was well tolerated. Our data provide a therapeutic rationale for the use of EGCG in acne.Journal of Investigative Dermatology advance online publication, 25 October 2012; doi:10.1038/jid.2012.292.
Skin aging involves degradation of extracellular matrix (ECM) in both the epidermal and dermal layers, it leaves visible signs on the surface of skin and the physical properties of the skin are modified. Chronological aging is due to passage of time, whereas premature aging occurred due to some environmental factors on skin produces visible signs such as irregular dryness, dark/light pigmentation, sallowness, severe atrophy, telangiectases, premalignant lesions, laxity, leathery appearance and deep wrinkling. There are several synthetic skincare cosmetics existing in the market to treat premature aging and the most common adverse reactions of those include allergic contact dermatitis, irritant contact dermatitis, phototoxic and photo-allergic reactions. Recent trends in anti-aging research projected the use of natural products derived from ancient era after scientific validation. Ample varieties of phytomolecules such as aloin, ginsenoside, curcumin, epicatechin, asiaticoside, ziyuglycoside I, magnolol, gallic acid, hydroxychavicol, hydroxycinnamic acids, hydroxybenzoic acids, etc. scavenges free radicals from skin cells, prevent trans-epidermal water loss, include a sun protection factor (SPF) of 15 or higher contribute to protect skin from wrinkles, leading to glowing and healthy younger skin. Present era of treating aging skin has become technologically more invasive; but herbal products including botanicals are still relevant and combining them with molecular techniques outlined throughout this review will help to maximize the results and maintain the desired anti-skin aging benefits.
We have investigated the modifying effects of epigallocatechin, a major polyphenolic constituent of green tea, on ultraviolet-A-activated gene expression in human fibroblasts and keratinocytes using the stress responsive enzymes: haem oxygenase-1, interstitial collagenase and cyclooxygenase-2. Although epigallocatechin strongly reduced ultraviolet-A-induced haem oxygenase-1 activation in skin-derived 'fibroblasts, the same compound activated collagenase and cyclooxygenase expression. In a keratinocyte cell line, ultraviolet-A-mediated haem oxygenase-1 over-expression was low and epigallocatechin failed to modulate it further. In contrast to the results with fibroblasts, ultraviolet-A activation of cyclooxygenase in keratinocytes was reduced by epigallocatechin. The results indicate that the effect of this green tea polyphenol on cellular stress responses is complex and may involve direct effects on signal transduction as well as changes that may be associated with its antioxidant activity.
Phenolic phytochemicals are the largest category of phytochemicals and the most widely distributed in the plant kingdom. The 3 most important groups of dietary phenolics are flavonoids, phenolic acids, and polyphenols. Flavonoids are the largest group of plant phenols and the most studied. Phenolic acids form a diverse group that includes the widely distributed hydroxybenzoic and hydroxycinnamic acids. Phenolic polymers, commonly known as tannins, are compounds of high molecular weight that are divided into 2 classes: hydrolyzable and condensed tannins. Quantification of food phenolics is just beginning, and preliminary results indicate high variability, even within a given food. Phenolics are biologically active compounds that may possess some disease-preventive properties. Evidence for their ability to prevent cancer or heart disease is preliminary and conflicting. The health benefits of phytochemicals have been reported in the popular press, and the public will come to dietitians for answers to their questions about phytochemicals.
The polyphenolic, flavonoid, and caffeine compositions of four commercial tea bag products (typical of those used in the UK, US, continental Europe, and the Middle East) and beverages prepared from them under a range of typical consumer use conditions have been studied. Leaf composition was determined by extraction with aqueous methanol: the absolute compositions of all four products were remarkably similar in terms of most phenolic compounds. The flavonoids comprised the major proportion (93-94%) of the total phenolics estimated by the Folin-Ciocalteu method. At brew times up to 2 min the composition of the brew solids was for each product practically independent of brew time, with flavonoids again comprising the major proportion (86-88%) of the total phenolics. The efficiency of extraction in brewing of total phenolics, total flavonoids, catechins, and theaflavins was up to 35-55% of the total available in the leaf, whereas the flavonol and flavone glycosides and caffeine were more efficiently extracted (up to 55-90%). The contribution of tea to the UK adult average total dietary intake of flavonols and flavones was calculated to be up to 80% depending on brewing conditions.
Cancer chemopreventive effects of polyphenols from green tea (GTP) in mouse models of photocarcinogenesis are established. The present study is extended from mouse model to human system in vivo to determine the effect of topical application of GTP to human individuals against UV light-induced DNA damage in the form of cyclobutane pyrimidine dimers (CPDs) in the skin. UVB-induced CPDs were detected by immunohistochemical technique using monoclonal antibodies to thymine dimers. With the gradual increase in UVB dose, both erythema response and CPD formation in the skin was increased. GTP treatment inhibited both UVB-induced erythema response as well as CPD formation. Topical treatment with GTP (approximately 1 mg/cm2 of skin area) 20 min before human buttock skin (sun-protected site) exposure to UVB inhibited CPD formation in epidermis by 81, 70, 60, and 60% at 0.5, 1.0, 2.0, and 4.0 minimal erythema dose of UV exposure, respectively. Treatment of human skin with varying doses of GTP (1-4 mg/2.5 cm2 of skin area) before a single dose of UVB exposure (4.0 minimal erythema dose) decreased dose dependently the formation of UVB-induced CPDs in both epidermis and dermis. The inhibition of UVB-induced CPDs by GTP treatment may be, at least in part, responsible for the inhibition of photocarcinogenesis. Our data suggest that GTP may be used as a novel chemopreventive candidate and possible strategy to reduce UV-induced skin cancer risk in the human population.
The purpose of this study was to investigate biomarkers of the bioavailability and metabolism of hydroxycinnamate derivatives through the determination of the pharmacokinetics of their urinary elimination and identification of the metabolites excreted. Coffee was used as a rich source of caffeic acid derivatives and human supplementation was undertaken. The results show a highly significant increase in the excretion of ferulic, isoferulic, dihydroferulic acid (3-(4-hydroxy-3-methoxyphenyl)-propionic acid), and vanillic acid postsupplementation relative to the levels presupplementation. Thus, ferulic, isoferulic, and dihydroferulic acids are specific biomarkers for the bioavailability and metabolism of dietary caffeic acid esters. Isoferulic acid is a unique biomarker as it is not a dietary component, however, dihydroferulic acid may well derive from other flavonoids with a structurally related B-ring. 3-Hydroxyhippuric acid has also been identified as an indicator for bioavailability and metabolism of phenolic compounds, and shows a highly significant excretion increase postsupplementation. The results reveal isoferulic acid (and possibly dihydroferulic acid) as novel markers of caffeoyl quinic acid metabolism.