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An Effect of Biofield Treatment on Multidrug-resistant Burkholderia cepacia: A Multihost Pathogen

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Abstract

Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B. cepacia was divided into two groups i.e. control and biofield treated. The analysis was done after 10 days of treatment and compared with control group. Control and treated group were analyzed for susceptibility pattern, MIC value, biochemical studies and biotype number using MicroScan Walk-Away® system. Sensitivity assay results showed a change in pattern from resistant to intermediate in aztreonam, intermediate to resistant in ceftazidime, ciprofloxacin, imipenem, and levofloxacin while sensitive to resistant in meropenem and piperacillin/tazobactam. The biofield treatment showed an alteration in MIC values of aztreonam, ceftazidime, chloramphenicol, ciprofloxacin, imipenem, levofloxacin, meropenem, piperacillin/tazobactam and tetracycline. Biochemical reactions of treated group showed negative reaction in colistin, lysine, and ornithine while positive reactions to acetamide, arginine, and malonate as compared to control. Overall results showed an alteration of 38.9% in susceptibility pattern, 30% in MIC values of tested antimicrobials and 18.2% change in biochemical reaction after biofield treatment. A significant change in biotype number (02063736) was reported with green pigment as special characteristics after biofield treatment as compared to control (05041776) group with yellow pigment. In treated group, a new species was identified as Pseudomonas aeruginosa, as compared to control. Study findings suggest that biofield treatment has a significant effect on the phenotypic character and biotype number of multidrug resistant strain of B. Cepacia.
An Effect of Biofield Treatment on Multidrug-resistant Burkholderia cepacia: A
Multihost Pathogen
Mahendra Kumar Trivedi
1
, Shrikant Patil
1
, Harish Shettigar
1
, Mayank Gangwar
2
, Snehasis Jana
2*
1
Trivedi Global Inc., 10624 S Eastern Avenue Suite A-969, Henderson, NV 89052, USA
2
Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal- 462026, Madhya Pradesh, India
*
Corresponding author: Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Hall-A, Chinar Mega Mall, Chinar Fortune City, Hoshangabad Rd., Bhopal-
462026, Madhya Pradesh, India, Tel: +91-755-6660006; E-mail: publication@trivedisrl.com
Rec date: Jun 29, 2015, Acc date: Jul 10, 2015, Pub date: Jul 17, 2015
Copyright: © 2015 Jana S et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in
immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients.
Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical
sample of B. cepacia was divided into two groups i.e. control and biofield treated. The analysis was done after 10
days of treatment and compared with control group. Control and treated group were analyzed for susceptibility
pattern, MIC value, biochemical studies and biotype number using MicroScan Walk-Away® system. Sensitivity
assay results showed a change in pattern from resistant to intermediate in aztreonam, intermediate to resistant in
ceftazidime, ciprofloxacin, imipenem, and levofloxacin while sensitive to resistant in meropenem and piperacillin/
tazobactam. The biofield treatment showed an alteration in MIC values of aztreonam, ceftazidime, chloramphenicol,
ciprofloxacin, imipenem, levofloxacin, meropenem, piperacillin/tazobactam and tetracycline. Biochemical reactions of
treated group showed negative reaction in colistin, lysine, and ornithine while positive reactions to acetamide,
arginine, and malonate as compared to control. Overall results showed an alteration of 38.9% in susceptibility
pattern, 30% in MIC values of tested antimicrobials and 18.2% change in biochemical reaction after biofield
treatment. A significant change in biotype number (02063736) was reported with green pigment as special
characteristics after biofield treatment as compared to control (05041776) group with yellow pigment. In treated
group, a new species was identified as Pseudomonas aeruginosa, as compared to control. Study findings suggest
that biofield treatment has a significant effect on the phenotypic character and biotype number of multidrug resistant
strain of B. cepacia.
Keywords:
Burkholderia cepacia
; Multidrug Resistant;
Antimicrobial Susceptibility; Biofield Treatment; Biochemical
Reactions; Biotyping
Introduction
Burkholderia cepacia (B. cepacia)
is an important human pathogen,
first isolated in cystic fibrosis patient and associated with serious
health issues such as wound infection, bacteremia, catheter-related
urinary infections and endocarditis [1].
B. cepacia
initially know as
Pseudomonas cepacia
referred as the phytopathogen responsible for a
bacterial rot of onions commonly found in soil and moist
environments [2].
B. cepacia
now emerged as an opportunistic human
pathogen especially for immunocompromised and hospitalized
patients [3].
B. cepacia
complex is the group of more than ten bacteria
with similar phenotypes but they differ genetically. They are
responsible for high morbidity and mortality rate of cystic fibrosis
patients mainly due to respiratory tract infections. Among this
B.
cepacia
complex, specifically
B. cenocepacia
is associated with serious
cepacia syndrome like high fever, overwhelming septicemia and
necrotizing pneumonia. Mortality rate among these patients are very
high as 62-100% [4].
During the last few decades, incidence of microbial resistance has
increased which leads to generates multi-drug-resistance (MDR)
organisms. Cases of MDR infection has been increased suddenly,
which leads to ineffective treatment and risk of spreading infections.
Resistance in microorganism against antimicrobials develops
naturally. Although MDR development is a natural phenomenon, but
extensive rise in the number of immunocompromised patients leads to
examine it and elucidate the molecular mechanism of organism during
infection [5]. In addition,
B. cepacia
is very difficult to treat due to its
highly resistant pattern against available antibiotics. Generally
combination therapy is preferred by the physicians, which consist of
meropenem along with other antibiotics such as amikacin,
minocycline or ceftazidime [6]. However, some pathogenic strains of
B. cepacia
are resistant to above drugs combination and are difficult to
treat. So, other treatment modalities must be adopted like multiple
combination bactericidal therapy to assess whether greater effect can
be achieved when more than two drugs are given together [7].
Recently, an alternate system called biofield treatment is reported to
alter the susceptibility of microorganism towards existing medicines
[8].
The conversion of mass into energy is well known in literature for
hundreds of years that was further explained by Fritz [9] and Einstein
[10]. The energy can exist in various forms that can be produced from
different sources such as potential, electrical, kinetic, magnetic, and
nuclear. Human nervous system consists of neurons that transmit
information in the form of electrical signals. Moreover, as per
Ampere-Maxwell law, electromagnetic field defines as when electrical
signals fluctuate will generate magnetic field with respect to time. It
involves electromagnetic bioinformation for regulating hemodynamics
(that is, the way the body system functions), hence it is known as
Journal of Tropical Diseases
Mahendra et al., J Trop Dis 2015, 3:3
http://dx.doi.org/10.4172/2329-891X.1000167
Research Article Open Access
J Trop Dis
ISSN:2329-891X JTD, an open access journal
Volume 3 • Issue 3 • 1000167
"biofield" [11]. Mr. Trivedi has the ability to harness the energy from
environment or Universe and can transmit into any living or nonliving
object(s). The objects always receive the energy and responding into
useful way that is called biofield energy and the process is known as
biofield treatment. Mr. Mahendra Trivedi biofield is well-known to
change the various physicochemical characteristics of metals and
ceramics [12-14]. The quality and yield of several agriculture products
have also been improved with several folds after biofield treatment
[15-17]. Exposure to biofield energy caused an increase in medicinal
property, growth, and anatomical characteristics of Ashwagandha
[18]. Further, the biofield treatment has considerably altered the
susceptibility of antimicrobials and biotype of microbe [19-20]. By
considering the above mentioned facts and literature reports on
biofield, the present work was undertaken to evaluate the impact of
biofield treatment on antimicrobials susceptibility, biochemical
reactions pattern, and biotype of MDR strain of
B. cepacia.
Material and Methods
Experimental design and biofield treatment
MDR strain of
B. cepacia
was collected from stored stock cultures of
clinical sample in Microbiology Lab, Hinduja Hospital, Mumbai. MDR
strain was divided in two groups i.e. control and treatment. Treatment
group, in sealed pack was handed over to Mr. Trivedi for biofield
treatment under laboratory conditions. Mr. Trivedi provided the
treatment through his energy transmission process to the treated
groups without touching the samples. The biofield treated sample was
returned in the similar sealed condition for further analysis on day 10
with respect to control using the standard protocols. After biofield
treatment, treated sample was analyzed for antimicrobial
susceptibility, biochemical reactions and biotype number using
MicroScan Walk-Away® (Dade Behring Inc., USA) and Negative Break
Point Combo (NBPC 30) panel with respect to control groups.
Evaluation of antimicrobial susceptibility assay
Antimicrobial susceptibility pattern of
B. cepacia
was studied using
MicroScan Walk-Away® NBPC30 as per manufacturer's instructions.
The antimicrobial susceptibility pattern (S: Susceptible, I:
Intermediate, and R: Resistant) and minimum inhibitory
concentration (MIC) values were determined by observing the lowest
antimicrobial concentration showing growth inhibition [21]. The
antimicrobials were procured from Sigma Aldrich, USA and used in
the susceptibility assay viz. amikacin, aztreonam, cefepime,
cefotaxime, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin,
gentamicin, imipenem, levofloxacin, meropenem, piperacillin,
piperacillin/tazobactam, tetracycline, ticarcillin/k-clavulanate,
tobramycin, and trimethoprim/sulfamethoxazole.
Biochemical reaction study
Biochemical study of
B. cepacia
was determined by MicroScan
Walk-Away® system in both control and treated groups. Biochemicals
were procured from Sigma Aldrich, USA and used in the study viz.
acetamide, adonitol, arabinose, arginine, cetrimide, cephalothin,
citrate, colistin, esculin hydrolysis, nitrofurantoin, glucose, hydrogen
sulfide, indole, inositol, kanamycin, lysine, malonate, melibiose,
nitrate, oxidation-fermentation, galactosidase, ornithine, oxidase,
penicillin, raffinose, rhaminose, sorbitol, sucrose, tartarate, tryptophan
deaminase, tobramycin, urea, and Voges-Proskauer [21].
Identification by biotype number
The biotype number of
B. cepacia
control and treated sample were
determined by MicroScan Walk-Away® processed panel data report
with the help of biochemical reaction data [21].
Results
Antimicrobial susceptibility
Results of antimicrobial sensitivity pattern and MIC of
B. cepacia
are summarized in Table 1 and 2 respectively. The biofield treatment
on MDR strain of
B. cepacia
showed a significant change in sensitivity
pattern of different antimicrobials such as ceftazidime, ciprofloxacin,
imipenem and levofloxacin changed from I→R. Aztreonam sensitivity
converted from R→I while meropenem and piperacillin/tazobactam
changed from S→R (Table 1).
S. No. Antimicrobial Control Treated
1 Amikacin R R
2 Aztreonam R I
3 Cefepime R R
4 Cefotaxime R R
5 Ceftazidime I R
6 Ceftriaxone R R
7 Chloramphenicol S -
8 Ciprofloxacin I R
9 Gentamicin R R
10 Imipenem I R
11 Levofloxacin I R
12 Meropenem S R
13 Piperacillin R R
14 Piperacillin/Tazobactam S R
15 Tetracycline R -
16 Ticarcillin/K-Clavulanate R R
17 Tobramycin R R
18 Trimethoprim/Sulfamethoxazole R -
Table 1: Effect of biofield treatment on
Burkholderia cepacia
to
antimicrobial susceptibility.
R: Resistant; I: Intermediate; S: Susceptible; ‘-‘: Not Reported
Decrease in MIC value was reported in case of aztreonam (16
µg/mL) and tetracycline (8 µg/mL) after biofield treatment as
compared to control. MIC value was increased after biofield treatment
in case of ceftazidime, chloramphenicol, ciprofloxacin, imipenem,
levofloxacin, meropenem, and piperacillin/tazobactam (Table 2).
Rest of the tested antimicrobials did not show any change in
sensitivity pattern and MIC value. Overall, study results showed a
Citation:
Mahendra KT, Shrikant P, Harish S, Mayank G, Jana S (2015) An Effect of Biofield Treatment on Multidrug-resistant Burkholderia
cepacia: A Multihost Pathogen. J Trop Dis 3: 167. doi:10.4172/2329-891X.1000167
Page 2 of 5
J Trop Dis
ISSN:2329-891X JTD, an open access journal
Volume 3 • Issue 3 • 1000167
change of 38.9% in susceptibility pattern and 30% in MIC values of
tested antimicrobials after biofield treatment. All these changes were
observed after 10 days of biofield treatment as compared to control
group.
S. No. Antimicrobial Control Treated
1 Amikacin >32 >32
2 Amoxicillin/ Clavulanic acid >16/8 >16/8
3 Ampicillin/Sulbactam >16/8 >16/8
4 Ampicillin >16 >16
5 Aztreonam >16 16
6 Cafazolin >16 >16
7 Cefepime >16 >16
8 Cefotaxime >32 >32
9 Cefotetan >32 >32
10 Cefoxitin >16 >16
11 Ceftazidime 16 >16
12 Ceftriaxone >32 >32
13 Cefuroxime >16 >16
14 Cephalothin >16 >16
15 Chloramphenicol ≤8 >16
16 Ciprofloxacin 2 >2
17 Gatifloxacin 4 -
18 Gentamicin >8 >8
19 Imipenem 8 >8
20 Levofloxacin 4 >4
21 Meropenem ≤4 >8
22 Moxifloxacin 4 -
23 Nitrofurantoin >64 >64
24 Norfloxacin >8 >8
25 Piperacillin >64 >64
26 Piperacillin/Tazobactam ≤16 >64
27 Tetracycline >8 8
28 Ticarcillin/K-Clavulanate >64 >64
29 Tobramycin >8 >8
30 Trimethoprim/Sulfamethoxazole >2/38 >2/38
Table 2: Minimum inhibitory concentration (MIC) of
Burkholderia
cepacia
for tested antimicrobials.
MIC values are presented in µg/mL; ‘-‘: Not Reported
Biochemical reaction
Table 3 summarizes the biochemical reactions. Biochemicals were
denoted with codes in control and biofield treated group on day 10.
Biochemical study showed positive reaction (i.e. from (-) negative to
(+) positive) in acetamide, arginine, and malonate while negative
reaction (i.e. from (+) positive to (-) negative) in case of colistin, lysine
and ornithine after biofield treatment as compared with control.
Overall, biochemical study showed the alteration of 18.2% after
biofield treatment.
S. No. Code Biochemical Control Treated
1 ACE Acetamide - +
2 ADO Adonitol - -
3 ARA Arabinose - -
4 ARG Arginine - +
5 CET Cetrimide + +
6 CF8 Cephalothin + +
7 CIT Citrate + +
8 CL4 Colistin + -
9 ESC Esculin hydrolysis - -
10 FD64 Nitrofurantoin + +
11 GLU Glucose - -
12 H2S Hydrogen sulfide - -
13 IND Indole - -
14 INO Inositol - -
15 K4 Kanamycin + +
16 LYS Lysine + -
17 MAL Malonate - +
18 MEL Melibiose - -
19 NIT Nitrate + +
20 OF/G Oxidation-
Fermentation
+ +
21 ONPG Galactosidase - -
22 ORN Ornithine + -
23 OXI Oxidase + +
24 P4 Penicillin + +
25 RAF Raffinose - -
26 RHA Rhaminose - -
27 SOR Sorbitol - -
28 SUC Sucrose - -
29 TAR Tartarate - -
Citation: Mahendra KT, Shrikant P, Harish S, Mayank G, Jana S (2015) An Effect of Biofield Treatment on Multidrug-resistant Burkholderia
cepacia: A Multihost Pathogen. J Trop Dis 3: 167. doi:10.4172/2329-891X.1000167
Page 3 of 5
J Trop Dis
ISSN:2329-891X JTD, an open access journal
Volume 3 • Issue 3 • 1000167
30 TDA Tryptophan
Deaminase
- -
31 TO4 Tobramycin + +
32 URE Urea - -
33 VP Voges-Proskauer - -
Table 3: Effect of biofield treatment on biochemical reactions of
Burkholderia cepacia
.
- (negative); + (positive)
Organism identification by biotype number
Biochemical tests result revealed a change in biotype number in
treated group on day 10 (02063736) with green pigmentation as a
special character as compared to control (05041776) which was having
a yellow pigmentation. In treated group, a new species was identified
as
Pseudomonas aeruginosa
, as compared to control (Table 4).
Feature Control Treated
Biotype 05041776 02063736
Organism Identification Burkholderia cepacia
Pseudomonas
aeruginosa
Characteristics Yellow pigment Green pigment
Table 4: Effect of biofield treatment on bio typing of
Burkholderia
cepacia
.
Discussion
B. cepacia
is a member of a group known as
B. cepacia
complex
causing “cepacia syndrome”, form of progressive necrotizing
pneumonia. It was associated with acute systemic infections and may
be fatal in some case [22]. The emergence of MDR of
B. cepacia
harbored a global health problem and an emerging drug resistant
microorganism commonly associated with immunocompromised
patients or patients with underlying lung disease, such as cystic
fibrosis. Due to continuous new drug discovery in antimicrobials, rate
of MDR microorganism increased causing serious health problems.
Cell membrane alterations in MDR microorganism results in
decreased uptake of antimicrobials [23], overexpression of drug target
enzymes results in mutation [24], and drug efflux pumps remains the
predominant mechanism in multi-drug resistant organisms [25].
Nowadays,
B. cepacia
acquires resistance against broad range of
antibiotics, so it was very difficult to start drug therapy in chronically
infected patients [26]. Due to this, use of combination therapy is
suggested rather than monotherapy against
B. cepacia
infection.
United States in 2002, demonstrated most prevalent infection among
B. cepacia
complex was
B. cepacia
followed by
B. multivorans
as the
next most dominant [27]. Contaminated disinfectants, ventilators,
antiseptics, and different types of medical equipment were also
responsible for
B. cepacia
infection. Even, person-to-person spread has
also been documented.
Best drug of choice in
B. cepacia
infection is co-trimoxazole,
followed by ceftazidime and meropenem, alone or in combination
with other antibiotics [28]. Experimental results showed a significant
alteration in sensitivity pattern after biofield treatment in azetronan,
ceftazidime, ciprofloxacin, imipenam, levofloxacin, meropenam, and
piperacillin/tazobactam. Apart from above antimicrobials, alteration
in MIC values were also reported in chloramphenicol and tetracycline.
Above results suggest that the Mr. Trivedi’s biofield has the ability to
harness energy from environment and can transmit it into
microorganism. However, biofield treatment varies according to
energy expressed, and information carried forward. Biofield treatment
in
B. cepacia
possibly made some alterations either in some enzymatic
pathways of microorganism or a change at genetic level, which leads to
alter the phenotypic features like sensitivity pattern and MIC values in
biofield treated group.
Several phenotypic identification tests were available to differentiate
the
Burkholderia
species. Experimental identification of
B. cepacia
was
performed using a series of biochemical analysis. Basic characteristics
of
B. cepacia
in biochemical reactions are presence of lysine, colistin,
oxidase activity, and ornithine decarboxylase activity. Pigment
production, hemolysis, and growth at 42°C temperature are other
general characters [29]. Biofield treatment showed a significant
alteration i.e. negative reaction in biochemical such as lysine, colistin,
and ornithine decarboxylase activity which are the basic characters of
B. cepacia
. Besides these changes, acetamide, arginine and malonate
showed positive reactions after biofield treatment. Biotyping was
performed using an automated system and found a significant
changed in biotype number (02063736) in treated group on day 10,
and organism identified as
Pseudomonas aeruginosa
after biofield
treatment as compared to control (biotype number, 05041776).
Pigment production is the special character of
B. cepacia
, biofield
treatment showed a significant change i.e. green pigment in treated
group as compared to yellow pigment in control (Table 4).
Biofield therapies in biomedical health care system are very popular
and claims to enhance human well-being and other metabolic
pathways [30]. National Center for Complementary and Alternative
Medicine (NCCAM), now defined biofield therapies in subcategory of
energy therapies as one of the five complementary medicine domain
[31]. Biofield treatment in microbiology was reported a significant
alteration in phenotypic characteristics of microorganism. Alteration
in microorganism might be due to the involvement of electromagnetic
field that acts on receptor protein at molecular or genetic level.
Biofield treatment, modifies ligand-receptor interaction which causes
alteration in phenotypic characters. Scientist studied that at extremely
low frequency, electromagnetic fields could alter transmembrane Ca2+
concentration of receptor proteins which causes damage and
developmental defects in different organs [32]. Results showed that,
biofield treatment induces changes in susceptibility pattern of
antimicrobials, MIC values, biochemical reactions, and biotype
number of MDR strain of
B. cepacia
.
Conclusion
Overall data concludes that biofield treatment has shown significant
impact on antimicrobial susceptibility pattern, MIC values,
biochemical reactions and biotype number of MDR strain of
B.
cepacia
. In treated group, a new species was identified as
Pseudomonas
aeruginosa
, as compared to control,
B. cepacia
. Based on the study
outcomes, biofield treatment could be applied to alter the sensitivity
pattern of antimicrobials, against multi drug resistance of
B. cepacia
.
Citation:
Mahendra KT, Shrikant P, Harish S, Mayank G, Jana S (2015) An Effect of Biofield Treatment on Multidrug-resistant Burkholderia
cepacia: A Multihost Pathogen. J Trop Dis 3: 167. doi:10.4172/2329-891X.1000167
Page 4 of 5
J Trop Dis
ISSN:2329-891X JTD, an open access journal
Volume 3 • Issue 3 • 1000167
Acknowledgement
Authors gratefully acknowledged the whole team of PD Hinduja
National Hospital and MRC, Mumbai, Microbiology Lab for their
support.
Conflict of Interest
The authors declare that they have no competing interest.
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Citation: Mahendra KT, Shrikant P, Harish S, Mayank G, Jana S (2015) An Effect of Biofield Treatment on Multidrug-resistant Burkholderia
cepacia: A Multihost Pathogen. J Trop Dis 3: 167. doi:10.4172/2329-891X.1000167
Page 5 of 5
J Trop Dis
ISSN:2329-891X JTD, an open access journal
Volume 3 • Issue 3 • 1000167
... Mr. Trivedi's unique biofield treatment is also known as The Trivedi Effect ® . Mr. Mahendra Trivedi's biofield treatment has been known to transform the structural, physical and thermal properties of several metals in material science [14][15][16], improved the overall productivity of crops [17,18], altered characteristics features of microbes [19][20][21] and improved growth and anatomical characteristics of various medicinal plants [22,23]. ...
... However, due to some limitation of science, the progress of new medications is slow and very challenging for scientists. Mr. Trivedi has the ability to harness energy from environment and altered the significant changes in microorganisms [19][20][21]. Mr. Trivedi's biofield treatment on various strains of clinical isolates [20] and multi drug resistance (MDR) [19,21] have recently reported a significant results. ...
... Mr. Trivedi has the ability to harness energy from environment and altered the significant changes in microorganisms [19][20][21]. Mr. Trivedi's biofield treatment on various strains of clinical isolates [20] and multi drug resistance (MDR) [19,21] have recently reported a significant results. Biofield treatment might be responsible to do alteration in microorganism at genetic level and/or enzymatic level, which may act on receptor protein. ...
Article
Full-text available
Enteric fever is a major global problem. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Salmonella paratyphi A (S. paratyphi A) in terms of antimicrobial susceptibility assay, biochemical characteristics and biotyping. S. paratyphi A strain were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 9150). The study was conducted in revived and lyophilized state of S. paratyphi A. Both revived (Group; Gr. II) and lyophilized (Gr. III) strain of S. paratyphi A were subjected to Mr. Trivedi’s biofield treatment. Revived treated cells was assessed on day 5 and day 10, while lyophilized treated cells assessed on day 10 after biofield treatment with respect to control (Gr. I). The antimicrobial susceptibility of S. paratyphi A showed significant (60%) alteration in revived treated cells (Gr. II) on day 10 as compared to control. The MIC values of S. paratyphi A also showed significant (53.12%) alteration in Gr. II and on day 10 while, no alteration was found in Gr. on day 5 as compared to control. It was observed that overall 18.18% biochemical reactions were altered in the treated groups with respect to control. Moreover, biotype numbers were substantially changed in Gr. II, on day 5 (53001040, S. paratyphi A), on day 10 (57101050, Citrobacter freundii complex) as compared to control (53001000, S. paratyphi A). Besides, biotype number was also changed in Gr. III (53001040, S. paratyphi A) as compared to control. The overall result suggested that biofield treatment had significant impact on S. paratyphi A in Gr. II on day 10 with respect to antimicrobial susceptibility, MIC values and biotype number.
... Burkholderia cepacia, a species that belongs to the Burkholderia cepacia complex (Bcc), has emerged as a multidrug-resistant opportunistic pathogen, causing severe human infections with a high rate of morbidity and mortality [1]. Many factors could contribute to B. cepacia dissemination in the hospital environment such as cross-transmission, frequent pulmonary procedures and to contacts with infected patients [2]. ...
Article
Burkholderia cepacia is well known as the causative agent of infections in humans where often shares niche with other pathogens, like Pseudomonas aeruginosa. Clinical isolate Burkholderia sp. BCC4135 was selected due to its strong quorum quenching (QQ) activity. Whole genome sequencing unveiled this isolate as B. cepacia with unique sequence type ST1485 and a myriad of genes belonging to resistome and virulome. Two QQ lactonases YtnP and Y2-aiiA originated from B. cepacia BCC4135 were cloned, expressed, and functionally characterized. They were active against a broad substrate spectrum of the N-acyl-homoserine lactones (AHLs). The YtnP lactonase was inactive, while Y2-aiiA was active against N-tetradecanoyl-dl-homoserine lactone (C14-HSL) which could imply the difference in their biological roles from the aspect of its quorum sensing (QS) autoregulation and interference with the QS systems of bacteria residing within the same niche. Both YtnP and Y2-aiiA were able to attenuate virulence potential of P. aeruginosa MMA83 clinical isolate declining its biofilm formation and virulence factors production. B. cepacia BCC4135 lactonases interfered with the las, rhl, and even pqs QS circuit of P. aeruginosa MMA83 transcription and the effect of combined enzymes was even more prominent. B. cepacia BCC4135 also employs the CepI/R QS system for governing its own virulence traits and possibly self-regulates the QQ/QS network through the different expression and activity of YtnP and/or Y2-aiiA. Our findings pointed out that BCC4135 lactonases could be exploited as an effective antivirulence drugs against P. aeruginosa and gave us a new insight into B. cepacia QQ/QS machinery.
... Various pre-clinical, in vitro cell lines, analytical, agricultural, and many more reports of The Trivedi Effect ® have been published so far. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported with significant transformation in the physicochemical properties of metals, chemicals, ceramics and polymers [14][15][16][17], improved overall agricultural crop yield [18][19][20], altered antimicrobial characteristics of various pathogenic microbes [21][22][23], improved biological activity of nutraceutical compounds in skin health [24,25], livestock [26], and many more. ...
Article
Full-text available
The objective of the present study was to evaluate the effect of Biofield Energy Healing on the plant callus, mouse and human derived Mesenchymal Stem Cells (MSCs) for their cell count and cellular proliferation. All the three type stem cells and DMEM media for the stem cell culture were divided into two parts, one received Biofield Energy Treatment (known as The Trivedi Effect®-Energy of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi through the Healer’s unique Energy Transmission process remotely and defined as Biofield Energy Treated (BT) group. On day 9 after treatment, the plant callus flasks of mandukparni, amla, and Centella were observed and visualized which showed an increased percentage in weight of the callus by 11.4%, 24.9%, and 51.7%, respectively as compared with the untreated groups. In addition, stem cell assay in murine bone marrow derived Mesenchymal Stem Cells (MSCs) showed an increased cellular proliferation by 115.2%, 127.6%, 161.9%, and 123.6%, in Biofield Energy Treated DMEM in the passage 1, 2, 3, and 4, respectively. Similarly, human derived Mesenchymal Stem Cells, Adipose-Derived (AD-MSC) cells showed a significant increased cell count by 139%, 119%, and 182% in the Biofield Energy Treated DMEM group with the passage 1, 2, and 3, respectively. Similarly, bone marrow (BM-MSC) cells showed a significant increased cell count by 102%, 170%, and 141% in the Biofield Energy Treated DMEM group with the passage 1, 2, and 3, respectively. However, BrdU assay for % cellular proliferation in the AD-MSC cells showed a significant increased cellular proliferation by 122%, 120%, and 184% in the passage 1, 2, and 3, respectively. On the other hand, BM-MSC cells showed a significant increased cellular proliferation by 125%, 146%, and 157% in the passage 1, 2, and 3, respectively. Hence, the results suggest that there was a significant growth in plant callus weight, mouse and human stem cell growth and proliferation after The Trivedi Effect®-Energy of Consciousness Treatment. It can be concluded that The Trivedi Effect® can be used as a complementary and alternate therapy for the stem cells regeneration, improvement of tissue injury that might be every effective against various autoimmune human diseases such as arthritis, osteoarthritis, and different neurodegenerative disorders.
... Hence, Biofield Energy Healing is increasingly accepted to promote human wellness as an alternative approach [12,13]. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported with significant revolution in the physicochemical properties of metals, chemicals, ceramics and polymers [14][15][16][17], improved agricultural crop yield, productivity, and quality [18][19][20], transformed antimicrobial characteristics of pathogenic microbes at genetic level [21][22][23], improved biological activity of nutraceutical compounds for skin health [24,25], livestock [26], and many more. ...
Article
Full-text available
The objective of the present study was to evaluate the effect of Biofield Energy Healing Treatment on the plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for their yield on day 7. All the three plant callus and MS liquid media were divided into two parts, one part was received Biofield Energy Treatment (known as The Trivedi Effect®-Energy Of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Alice Branton through the Healer’s unique Energy Transmission process remotely and defined as Biofield Energy Treated group. Another part did not receive any treatment and defined as the control group. On day 7 after the treatment, the flasks were observed and visualized under the phase contrast microscope for monitoring the cell growth and photomicrographs were captured at 200X magnification along with the wet weight of the callus cultures. Experimental data suggested that all the three plants viz. Mandukparni, Katsarika, and Amla callus showed significantly improved growth rate after seven days as compared with the respective untreated groups. The growth of the callus (weight in mg) of Mandukparni, Katsarika and Amla callus after 7 days of the Biofield Energy Treatment was 289.7, 348.5, and 512 mg respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika, and Amla was 3.3%, 7.7%, and 79.6%, respectively as compared with the untreated group. Hence, the results indicated that there was a significant growth observed after The Trivedi Effect®-Energy of Consciousness Treatment in the tested plant stem cells. It can be concluded that The Trivedi Effect® would be used as a complementary and alternative treatment to maximize most valuable natural products yield and quality best quality such as herbal medicinal plants, food and dietary supplements, flavoring and coloring agents, cosmetics and fragrance products, and many more.
... Hence, Biofield Energy Healing is increasingly accepted to promote human wellness as an alternative approach [12,13]. The Trivedi Effect ® -Consciousness Energy Healing Treatment has been reported with significant revolution in the physicochemical properties of metals, chemicals, ceramics and polymers [14][15][16][17], improved agricultural crop yield, productivity, and quality [18][19][20], transformed antimicrobial characteristics of pathogenic microbes at genetic level [21][22][23], improved biological activity of nutraceutical compounds for skin health [24,25], livestock [26], and many more. ...
Article
Full-text available
This study investigated the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment on the plant stem callus of Mandukparni (Centella asiatica), Katsarika (Baleria cristata), and Amla (Phyllanthus emblica). Each stem cell callus and MS liquid media were divided into two parts. One part of each was denoted as control. The other part was received the Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was defined as the Biofield Energy Treated sample. The weight of mandukparni callus was significantly increased by 8.5% in Biofield Energy Treated group (304.5 mg) compared to control group (280.6 mg). Furthermore, weight of katsarika stem cell callus was significantly increased by 38.6% in Biofield Energy Treated group (448.7 mg) compared to control (323.7 mg). The weight of amla stem cell callus was significantly raised by 41.4% in the Biofield Energy Treated group (403 mg) compared to control group (285 mg). Overall, the data suggest that there was a significant growth and yields of plants callus after treatment with the Biofield Energy. Therefore, The Trivedi Effect® - Consciousness Energy Healing approach could be useful for the production of more active constituents present in the three plants callus in a cost-effective manner and can be useful for food and dietary supplements, flavoring and coloring agents, cosmetics and fragrance products.
... Energy Healing Treatment has been reported with significant revolution in the physicochemical properties of metals [14][15][16][17], improved agricultural crop yield, productivity, and quality [18][19][20], transformed antimicrobial characteristics of pathogenic microbes at genetic level [21][22][23], improved biological activity of nutraceutical compounds for skin health [24,25], livestock [26], biotechnology [27,28], improved bioavailability [29][30][31], nutraceuticals [32,33], cancer research [34,35], bone health [36][37][38], human health and wellness. ...
Article
Full-text available
The present study was aimed to evaluate the effect of Consciousness Energy Healing Treatment on plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for its growth and yield on day 7. The plant callus of all the three plants were divided into two parts, one part received Biofield Energy Treatment remotely (known as The Trivedi Effect®-Energy of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Gopal Nayak and denoted as Biofield Energy Treated group, while another part did not receive any treatment and defined as control. Plants callus were observed on day 7 after treatment and visualized under phase contrast microscope for cell growth, captured photomicrographs along with wet weight compared with day 0. Results suggested that three plants viz. Mandukparni, Katsarika, and Amla callus after 7 days showed a significant improved growth rate as compared with the respective untreated groups. The weight of the callus growth (in mg) after Biofield Energy Treatment among Mandukparni, Katsarika, and Amla callus on day 7 was 630, 782, and 920 mg, respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika and Amla was 11.1%, 52.4%, and 68.5%, respectively than untreated. Hence, results concluded a significant growth in callus weight after Biofield Energy Treatment, which suggested that it could be used as complementary and alternate approach in order to produce most high-value phytoconstituents from plant callus that can be used for making various drugs, food flavoring and colouring agents.
... Mr. Trivedi is well known to possess the unique biofield energy treatment (The Trivedi Effect ® ) which is reported to alter the properties such as growth and yield of plants in the field of agriculture [24,25]. The effect was also reported on phenotypic characters of microorganisms in the field of microbiology [26,27]. Hence, the present study was designed to analyse the impact of biofield energy treatment on various physicochemical properties of BHT using XRD, DSC, TGA/DTG, FT-IR and UV-Vis spectroscopic techniques. ...
Article
The antioxidants play an important role in the preservation of foods and the management of oxidative stress related diseases by acting on reactive oxygen species and free radicals. However, their use in high temperature processed food and pharmaceuticals are limited due to its low thermal stability. The objective of the study was to use the biofield energy treatment on butylated hydroxytoluene (BHT) i.e. antioxidant and analyse its impact on the physical, thermal, and spectral properties of BHT. For the study, the sample was divided into two groups and termed as control and treated. The treated group was subjected to biofield energy treatment. The characterization of treated sample was done using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) and UV-visible (UV-Vis) spectroscopy. The XRD results showed the alteration in lattice parameters, unit cell volume, and molecular weight along with 14.8% reduction in the crystallite size of treated sample as compared to the control. The DSC analysis showed an increase in the latent heat of fusion from 75.94 J/g (control) to 96.23 J/g in the treated BHT sample. The TGA analysis showed an increase in onset temperature of decomposition (130°C→136°C) and maximum thermal decomposition temperature (152.39°C→158.42°C) in the treated sample as compared to the control. Besides, the FT-IR analysis reported the shifting of aromatic C-H stretching peak towards higher frequency (3068→3150 cm-1) and C=C stretching towards lower frequency (1603→1575 cm-1) as compared to the control sample. Moreover, the UV spectrum also revealed the shifting of the peak at λmax 247 nm (control) to 223 nm in the treated sample. The overall results showed the impact of biofield energy treatment on physical, thermal and spectral properties of BHT sample.
... Recent studies reported that the uses of energy medicine provided the highest benefit to cancer patients as compared to the use of other Complementary and Alternative Medicine (CAM) [16]. The Trivedi Effect ® -Unique Biofield Energy Treatment has been known to transform the structural, physical, and thermal properties of several metals in materials science [17][18][19][20], improved the overall productivity of crops [21,22], altered characteristics features of microbes [23][24][25] and improved growth and anatomical characteristics of various medicinal plants [26,27]. Based on the data from the literature of the Biofield Energy Treatment, authors designed this study to evaluate the impact of Consciousness Energy Healing based DMEM and test herbomineral formulation on various skin health parameters using human foreskin fibroblast (HFF-1), human keratinocytes (HaCaT), and mouse melanoma (B16-F10) cell lines. ...
Article
Full-text available
The aim of this study was to evaluate the effect of the Consciousness Energy Healing based test formulation and cell medium (DMEM) against various skin health parameters. The test formulation and DMEM were divided into two parts. One part received the Consciousness Energy Healing Treatment (The Trivedi Effect®) by Deborah Lea Smith and termed as the Biofield Energy Treated sample, while other part denoted as untreated test item. The study was assessed for the measurement of collagen, elastin, hyaluronic acid, melanin synthesis, cell viability against UV-B induced stress, and wound healing activity. MTT assay showed that the test formulation was safe and nontoxic in three cell lines. Bromodeoxyuridine (BrdU) assay data showed that the percent cell proliferation was increased significantly by 22.69% and 34.99% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 8.75 µg/mL compared to the UT-DMEM + UT-Test formulation group. Collagen was increased by 15.12% and 15.26% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 0.625 µg/mL compared to the untreated group. Elastin was significantly increased by 9.10%, 13.71%, and 12.41% in the UT-DMEM + BT-Test formulation, BT-DMEM + Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 5 µg/mL compared to the untreated group. Elastin was also increased significantly by 17.78% and 6.01% in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively at 10 µg/mL compared to the untreated group. Hyaluronic acid was significantly increased by 3.90%, 5.37%, and 6.82% in the UT-DMEM + BT-Test formulation at 0.625, 1.25, and 2.5 µg/mL, respectively compared to the untreated group. Melanin level was reduced by 14.64% and 18.25% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 0.13 µg/mL compared to the untreated group. Skin cells protection was significantly increased by 21.32%, 23.81%, and 7.20% at 0.625, 1.25, and 2.5 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared to the untreated group. Wound healing assay exhibited significant wound closure and cell migration in all the tested groups compared to the untreated group. Overall, the Biofield Energy Treated test formulation and DMEM (The Trivedi Effect®) were found to be safe and can be developed as an effective cosmetic product to protect and treat the skin problems including infection, photosensitivity, acne, hives, chickenpox, eczema, rosacea, seborrheic dermatitis, psoriasis, erythema, contact dermatitis, skin aging, wrinkles, etc.
... Biofield Energy Healing (The Trivedi Effect ® ) has been described worldwide with remarkable results in nonliving materials and in living organisms. The Trivedi Effect ® has been found with momentous results in the field of microbiology [15][16][17], agriculture science [18,19], livestock [20], and materials science [21][22][23][24]. With this diversifying results and application of Biofield Energy Healing, a new Biofield Energy Healing based herbomineral formulation was designed for skin health, which was tested in cell lines such as HFF-1, HaCaT, and B16-F10 cell lines. ...
Article
Full-text available
Alternative or natural systems of medicine are practiced worldwide that mostly emphasize research of herbal formulations, which might be helpful against various disorders. The objective of this study was to evaluate the influence of The Trivedi Effect®-Consciousness Energy Healing Treatment on an herbomineral test formulation and cell medium (DMEM) for the skin health. The formulation contained minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two equal parts. One part received Biofield Energy Treatment (BT) by William Dean Plikerd and other part was denoted as the untreated test items (UT). MTT assay showed that the test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. The cell proliferation using BrdU method showed improved cell proliferation by 43.63% and 10.97% in the BT-DMEM + UT-Test formulation and BT-DMEM + BT-Test formulation group, respectively at 17.5 µg/mL compared with the untreated group. The collagen level was significantly increased by 2.65% and 23.56% at 2.5 and 1.25 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 8.34% at a concentrations of 2.5 µg/mL in the BT-DMEM + BT-Test formulation group compared with the untreated group. However, the hyaluronic acid (HA) level was increased by 2.60% at 0.625 µg/mL in the BT-DMEM + UT-Test formulation group compared with the untreated group. Moreover, melanin synthesis was decreased by 14.22% and 17.61% in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at a concentration of 0.125 µg/mL. Anti-wrinkling activity in HFF-1 cells showed improve cell viability by 14.54% and 11.32% at 1.25 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively compared with the untreated group. Wound healing scratch assay results showed significantly increased healing rates by 10.5% and 8.3% in the HFF-1 and HaCaT cells lines, respectively in the Biofield Energy Healing based test formulation. In conclusion, The Trivedi Effect® based test formulation and DMEM could be useful to improve the skin ECM components, along with protection against UV-B rays, which could be useful for the development of an effective cosmetic product for the prevention and treatment of several skin problems such as erythema, contact dermatitis, skin aging, wrinkles and/or change in the skin color, etc. as an anti-ageing, skin-whitening, and anti-wrinkling.
Article
Objective The burden of healthcare-associated infections (HAIs) is higher in low- and middle-income countries, but HAIs are often missed because surveillance is not conducted. Here, we describe the identification of and response to a cluster of Burkholderia cepacia complex (BCC) bloodstream infections (BSIs) associated with high mortality in a surgical ICU (SICU) that joined an HAI surveillance network. Setting A 780-bed, tertiary-level, public teaching hospital in northern India. Methods After detecting a cluster of BCC in the SICU, cases were identified by reviewing laboratory registers and automated identification and susceptibility testing outputs. Sociodemographic details, clinical records, and potential exposure histories were collected, and a self-appraisal of infection prevention and control (IPC) practices using assessment tools from the World Health Organization and the US Centers for Disease Control and Prevention was conducted. Training and feedback were provided to hospital staff. Environmental samples were collected from high-touch surfaces, intravenous medications, saline, and mouthwash. Results Between October 2017 and October 2018, 183 BCC BSI cases were identified. Case records were available for 121 case patients. Of these 121 cases, 91 (75%) were male, the median age was 35 years, and 57 (47%) died. IPC scores were low in the areas of technical guidelines, human resources, and monitoring and evaluation. Of the 30 environmental samples, 4 grew BCC. A single source of the outbreak was not identified. Conclusions Implementing standardized HAI surveillance in a low-resource setting detected an ongoing Burkholderia cepacia outbreak. The outbreak investigation and use of a multimodal approach reduced incident cases and informed changes in IPC practices.
Article
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Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mr.Trivedi's energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through an automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi's energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
Article
Full-text available
This study tested the Null Hypothesis for the effect of BioField Energy applied to two separate crops under typical growing conditions, namely ginseng and organic blueberry in commercial plantings in Wisconsin and California, respectively. Following treatment to replicated plots in standard experimental design, data were collected at harvest for yield quantity and quality. Ginseng plants treated both pre-harvest and a combination of pre- and post-harvest showed market grade increases of 33.3% and 40.0%, respectively. Point of sale gross return for this crop is dependent upon tuber quality, and from these data the economics of these treatments were calculated. Based on stand adjusted yields and quality values, a combination of pre- and post-harvest treatment increased gross income by 57.4%. The second crop showed similar trends in positive responses. In the two blueberry varieties studied, Emerald treated plants showed 96% statistical increase in yield, while Jewel showed 31% increase. At the time of treatment, each variety was in a different stage of flowering. The Emerald variety was in the flowering stage, and Jewel was predominately in the fruiting stage. Both treated cultivars however demons-trated increased yield quantity and quality. The specific mechanisms that lead to these pre-liminary results need further investigation.
Article
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Background : While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Enterococcus faecalis [ATCC –51299], report the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists Results: The results indicated that Mr.Trivedi’s energy has changed 9 of 27 biochemical characteristics of Enterococcus faecalis along with significant changes in susceptibility pattern in 5 of 31 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy/treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
Article
Full-text available
Purpose While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiment on Staphylococcus epidermis [ATCC –13518], validate the effects of such energy transmitted through a person, Mr. Mahendrakumar Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. All tests were performed with the help of automation on the Microscan Walkaway System in Microbiology Laboratory - accredited by The College of American Pathologists. Results The results indicated that Mr.Trivedi’s energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. The Biotype number has changed from the original control strain giving rise to 2 different biotypes in treated samples while the external energy /treatment given was the same for all treated samples suggestive of random polymorphism as analyzed through the automated machine. Conclusions These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner. In lyophilized state, biochemical and enzymatic characteristics could be altered.
Article
Full-text available
Soybean production in Iowa USA is among the most productive for rainfed regions in the world. Despite generally having excellent soils, growing season temperatures and rainfall, soybean yields are decreased by weed interference and inadequate available soil water at key stages of crop development. A field study was conducted at two locations in Iowa in 2012 to determine if seed-applied fungicide or biofield treatments influenced weed community, soil volumetric water concentration and soybean yield and quality. Application of biofield treatment resulted in lower density of tall waterhemp density, greater soybean stand density at R8 stage and greater seed pod–1 compared to the absence of seed fungicide and biofield. Soil volumetric water content varied by seed fungicide x biofield x date interaction but differences were not consistent among treatment combinations. Overall, seed fungicide and biofield treatments had similar effects on soybean productivity, however additional research is necessary to determine if biofield treatment is a suitable replacement for seed fungicide application.
Article
Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B. cepacia was divided into two groups i.e. control and biofield treated. The analysis was done after 10 days of treatment and compared with control group. Control and treated group were analyzed for susceptibility pattern, MIC value, biochemical studies and biotype number using MicroScan Walk-Away® system.Sensitivity assay results showed a change in pattern from resistant to intermediate in aztreonam, intermediate to resistant in ceftazidime, ciprofloxacin, imipenem, and levofloxacin while sensitive to resistant in meropenem and piperacillin/ tazobactam.The biofield treatment showed an alteration in MIC values of aztreonam, ceftazidime, chloramphenicol, ciprofloxacin, imipenem, levofloxacin, meropenem, piperacillin/tazobactam and tetracycline. Biochemical reactions of treated group showed negative reaction in colistin, lysine, and ornithine while positive reactions to acetamide,arginine, and malonate as compared to control. Overall results showed an alteration of 38.9% in susceptibility pattern, 30% in MIC values of tested antimicrobials and 18.2% change in biochemical reaction after biofield treatment. A significant change in biotype number (02063736) was reported with green pigment as special characteristics after biofield treatment as compared to control (05041776) group with yellow pigment. In treated group, a new species was identified as Pseudomonas aeruginosa, as compared to control. Study findings suggest that biofield treatment has a significant effect on the phenotypic character and biotype number of multidrug resistant strain of B. cepacia.
Article
Quantum mechanics was developed when human energies of consciousness were found to influence observations at the scale of elementary particles, here referred as non-contact biofield treatment or biofield energies. Quantum mechanics has also proved ef-ficacious in biological processes. The present experiments found an enhanced and significant impact of the biofield treatment on adaptive micropropagation response and callus induction of two plant species , Withania somnifera and Amaranthus dubius. The enhancement was perhaps due to greater focus on adaptation rather than specific mechanisms, showing high potential including at biochemical and genetic levels. Possible reasons for the enhancement are discussed and a possible model is presented, consistent with current scientific theory.
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Recent studies report the effect of biofield treatment on changes in structural characteristics of organic and inorganic matter, on cancer cells in vitro and on overall plant development. This study tested the impact of the same treatment applied to lettuce and tomato seeds and transplants (Lactuca sativa var. capitata and Lycopersiconesculentum var. Roma) in commercial plantings with and without fertilizers and pesticides, in relation to yield, quality, and pest inhibition. Treated lettuce plants with fertilizer and pesticide applications were more vigorous, exhibited less incidence of soil-borne fungal wilt, and subsequent yield was statistically greater 43% compared to untreated plants. Treated plants with no fertilizer or pesticide applications in the field behaved similarly to untreated plants that received routine fertilizer and pest control inputs. Similarly, fertilizer applied and fertilizer non-applied treated tomato plants exhibited a 25% and 31% increase in total observable yields respectively. Treated tomato and lettuce plants also measured higher in total leaf tissue chlorophyll content. The combination of biofield treatment along with administration of chemical additives demonstrated the best results with statistically increased yields and higher pest resistance in both test cropping systems. The specific mechanisms that lead to these preliminary results have yet to be determined.
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Systemic fungal infections contribute to at least 10% of deaths in hospital settings. Most antifungal drugs target ergosterol (polyenes) or its biosynthetic pathway (azoles and allylamines), or beta-glucan synthesis (echinocandins). Antifungal drugs that target proteins are prone to the emergence of resistant strains. Identification of genes whose mutations lead to targeted resistance can provide new information on those pathways. We used Aspergillus nidulans as a model system to exploit its tractable sexual cycle and calcofluor white as a model antifungal agent to cross-reference our results with other studies. Within 2 weeks from inoculation on sublethal doses of calcofluor white, we isolated 24 A. nidulans adaptive strains from sectoring colonies. Meiotic analysis showed that these strains had single-gene mutations. In each case, the resistance was specific to calcofluor white, since there was no cross-resistance to caspofungin (echinocandin). Mutation sites were identified in two mutants by next-generation sequencing. These were confirmed by reengineering the mutation in a wild-type strain using a gene replacement strategy. One of these mutated genes was related to cell wall synthesis, and the other one was related to drug metabolism. Our strategy has wide application for many fungal species, for antifungal compounds used in agriculture as well as health care, and potentially during protracted drug therapy once drug resistance arises. We suggest that our strategy will be useful for keeping ahead in the drug resistance arms race.