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Canine Olfaction: Scent, Sign, and Situation

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Canine olfaction is a rich field of study for the behavioural sciences and neurosciences, and it is rich in interdisciplinary connections. This chapter will explore the neurocognitive and neuroconative bases of olfaction (the neurophysi-ological foundations of cognition and motivation), and discuss the behavioural, psychological, and semiotic dimensions of scent processing. It will cover the basic psychophysics of olfaction and the methodologies allowing us to explore this sensory modality, as well as the complex cognitive and motivational dimensions of scent. This chapter will open with an overview of the different disciplines involved in the study of canine olfaction. Some basic anatomy and neuroscience will be reviewed, mostly with direct reference to behaviour and associated psychological processes (e.g., cognitive, motivational, and affective systems). For the behavioural aspect of olfaction, a discussion of the contrasting, yet complementary methods of ethology and experimental psychology will be examined. The importance of both field and laboratory research will be highlighted. Olfaction ''in context'' will also be discussed in reference to zoosemiotics and in order to understand the canine olfactory psychoethology in its most meaningful and functional dimension: processing ''signs'' (including symptoms as with dogs trained for biomedical applications such as symptom detection). We will conclude with a short commentary on the human-canine sensory symbiosis with sniffer dogs.
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Chapter 1
Canine Olfaction: Scent, Sign,
and Situation
Simon Gadbois and Catherine Reeve
Abstract Canine olfaction is a rich field of study for the behavioural sciences and
neurosciences, and it is rich in interdisciplinary connections. This chapter will
explore the neurocognitive and neuroconative bases of olfaction (the neurophysi-
ological foundations of cognition and motivation), and discuss the behavioural,
psychological, and semiotic dimensions of scent processing. It will cover the basic
psychophysics of olfaction and the methodologies allowing us to explore this
sensory modality, as well as the complex cognitive and motivational dimensions of
scent. This chapter will open with an overview of the different disciplines involved
in the study of canine olfaction. Some basic anatomy and neuroscience will be
reviewed, mostly with direct reference to behaviour and associated psychological
processes (e.g., cognitive, motivational, and affective systems). For the behavioural
aspect of olfaction, a discussion of the contrasting, yet complementary methods of
ethology and experimental psychology will be examined. The importance of both
field and laboratory research will be highlighted. Olfaction ‘in context’’ will also be
discussed in reference to zoosemiotics and in order to understand the canine
olfactory psychoethology in its most meaningful and functional dimension: pro-
cessing ‘‘signs’’ (including symptoms as with dogs trained for biomedical appli-
cations such as symptom detection). We will conclude with a short commentary on
the human-canine sensory symbiosis with sniffer dogs.
1.1 The Sciences of Canine Olfaction
Canids, like most mammals (and many other vertebrates, such as reptiles), live in an
olfactory world. Their Umwelt, or ‘‘sensory world’’, is impressively different from
ours (see also Horowitz and Hecht, this volume). Observing our dogs exploring
S. Gadbois (&)C. Reeve
Department of Psychology and Neuroscience, Neuroscience Institute, Dalhousie
University, Halifax, NS, Canada
e-mail: sgadbois@dal.ca
A. Horowitz (ed.), Domestic Dog Cognition and Behavior,
DOI: 10.1007/978-3-642-53994-7_1, Springer-Verlag Berlin Heidelberg 2014
3
their social landscape by relentlessly sniffing scent marks (mostly invisible, unless
you live where yellow snow is a possibility) left by others, we understand that we
are not in the same sensory-perceptual world. We are just starting to understand the
amount of information that animals process from chemical messages. Like us with
our emails and social site postings, dogs and other canids have their own world of
‘peemails’’ and ‘‘Nosebook’’ to explore, create and manipulate (Harrington and
Asa 2003; Bekoff 2001; Allen et al. 1999; Wells and Bekoff 1981).
The study of olfaction has traditionally focused on mammals and insects. In
mammals, rats and mice have been the primary model systems, at least in
experimental psychology and neuroscience. Recent focus on dogs, and working
dogs more specifically, seems to have sparked an interest in the scientific study of
olfactory processing in canines. Different theoretical, conceptual, and methodo-
logical perspectives have contributed to the science of canine olfaction over the
past century or so, either directly or indirectly. Here, we will advocate a generalist,
synthetic, and broad-reaching perspective on canine olfaction. We believe in a full
integration of experimental psychology (mostly psychophysics and animal learn-
ing theory), behavioural biology (mostly ethology because of its strong focus on
proximate questions), neuroscience (behavioural, cognitive, affective, and social)
and zoosemiotics. Many new applications surface every year (from bed bug
detection to telephone poll rot detection)—most either unknown by the scientific
community, or known in their own parallel (non-academic) world of ‘‘research and
development’’ (R&D) and applied types of research with low inter-disciplinary
diffusion. An important part of the scientific contribution to canine olfaction has
been fringe and marginal for decades. Canine olfaction can be discussed in relation
to the natural environment of the animal or in the context of laboratory conditions.
Applied canine olfaction is also a growing area of investigation and often relates to
‘quasi-experimental’’ approaches and the industrial, R&D model of applied
research. We will discuss some of these applications later.
The field of ‘zoosemiotics’ deserves a brief introduction. Sebeok (1968,1977)
conceptualized the field around the idea of ‘semiotics’, or the ‘theory of signs’. This
perspective applies well to ‘‘semiochemicals’’: chemicals used as signs. At first
glance, the field seems to duplicate the study of animal communication (e.g.,
Bradbury and Vehrencamp 2011). But interestingly, communication, according to
zoosemioticians, is only one of three sign processes, or semiosis. Communication
describes the ‘‘classical’’ perspective in the field of ethology: exchange of infor-
mation between a sender and a receiver. Zoosemiotics also makes room for ‘‘rep-
resentation’’ (when a sender is producing a sign without the presence of a receiver,
arguably for an intended receiver or clearly identified receiver, or if you will, a ‘‘to
whom it may concern’’ message) and ‘‘signification’’ (when a receiver is present
and processing a sign, without the emitter or sender being present). A clear case of
representation would be when a canine sender/emitter is urinating, potentially scent
marking its territory, but without any other dog in the vicinity. Signification would
be when a urine mark or defection is found, and processed by the receiver, without
the sender/emitter present, and without the assumption that the receiver was the
intended target (see also Bekoff, this volume, on urination patterns). The elegant
4 S. Gadbois and C. Reeve
nuance here is that intentionality in the processing of pheromones or allomones
(pheromones crossing the species barrier) is not assumed in either signification or
representation. In other words, a ‘‘sign’’ (including an olfactory one, or semio-
chemical) can provide information without being necessarily produced in the
context of communication (in which case, the term ‘‘signal’’ is used).
This brings us to the title of this chapter, ‘‘Canine Olfaction: Scent, Sign, and
Situation’’. It goes without saying that the stimuli discussed here are odorants or
scents. They are signs as defined by zoosemiotics, and they are always in context.
Without having to take a radical ‘‘ecological’’ or behavioural ecology perspective
on the issue, ethology, with its focus on direct observation of observable
behaviours, and its interest in social and developmental issues, as well as neuro-
physiological and fine-grained analysis of motor patterns (Fentress and Gadbois
2001), provides tools and an ‘‘in-context’’ framework that complements zoo-
semiotics, not to mention the highly formalist approaches of psychophysics and
animal learning research.
1.2 A Neurocognitive and Neuroconative Perspective
on Olfaction
This section will address how mental processes (cognition) and motivations
(conation) interact to produce and modulate olfactory behaviours. The neurosci-
ence of mammalian olfaction is a vast area of research, but the work on canines is
limited, mostly because of the potential invasiveness of the research. Much of what
will be mentioned here emanates from rodent and human research: most verte-
brates share the fundamental structure of the olfactory system, as well as its
mechanisms, and the homogeneity within the mammalian brain is truly impressive
(Panksepp 1998; Panksepp and Biven 2012). The olfactory system is fundamen-
tally linked to the limbic system or paleo-mammalian brain (MacLean 1990). Our
perspective in this chapter is neurocognitive. We will discuss two areas of theo-
retical interest in our lab: the neurocognitive issues behind olfactory processing
and learning in relation to training scent processing canines and the issue of
learning and motivation from a ‘‘soft’’ pharmacological perspective that one of us
(SG) calls the ‘‘dopamine hypothesis’’ (Gadbois 2010).
Because of our neurocognitive focus we will redirect the reader interested in the
neuroanatomical and neurophysiological foundations of olfaction to excellent
reviews (e.g., Buck 2000; Menini 2009; Shepherd 1994; Wilson and Stevenson
2006; Zelano and Sobel 2005). For the purpose of this chapter, we will focus mostly
on the olfactory cortex per se (Haberly 1998; Price 2003), that is, the cortical and
peri-cortical part of the olfactory system (the neo-mammalian and paleo-mamma-
lian parts of the olfactory system, respectively, per MacLean’s (1990) terminology).
We will not get into the distinction between the primary (or main) olfactory system
1 Canine Olfaction: Scent, Sign, and Situation 5
and secondary (or accessory) olfactory system, well known from countless reviews
on the mammalian vomeronasal organ (VNO) and processing of pheromones and
allomones, but we will discuss one important neurocognitive distinction in olfactory
processing: the distinction between smelling (implicit processing, involuntary) and
sniffing (explicit processing, exploratory) as explained by Sobel et al. (1998).
Although Sobel et al. studied human brains, it is reasonable to think that some
aspects of this distinction between implicit and explicit neurocognitive processing
could take place in canids.
1.2.1 Three Main Neuroanatomical Components
of Olfactory Cognition and Conation
We will now describe briefly three main components of the olfactory system that
are involved in more ‘‘cognitive’’ processing of olfactory information, as well as
being involved in motivational mechanisms underlying olfaction.
1.2.1.1 Pyriform Cortex
The pyriform cortex is also called the ‘prepyriform cortex’ or ‘primary olfactory
cortex’: information from the olfactory bulb (an integral component of the limbic
system and the first sub-system involved in processing olfactory information from
the primary and secondary olfactory systems) is directly wired to the pyriform
cortex, which then feeds information to the frontal lobes and the orbitofrontal
cortex (via the thalamus). The pyriform cortex is believed to play an important role
in olfactory detection and discrimination (Price 2003; Wilson and Sullivan 2011),
especially the posterior pyriform cortex. (The anterior pyriform cortex is more
involved in the basic analysis of the chemical structure of the odorant. See Sect.
1.3.1.1 below).
1.2.1.2 Entorhinal Cortex
The entorhinal cortex feeds directly into the hippocampus, frontal cortex, and
orbitofrontal cortex. It has an important role in memory, especially spatial mem-
ory, and thus may play an important role in navigation and possibly tracking and
trailing in dogs. It is often defined as the main interface between the hippocampus
and the neocortex (frontal and orbitofrontal cortices). The structure is also linked
to the amygdala and seems to be involved in autonomic nervous system responses
to odours. Emotional memories driven or triggered by smell may involve the
entorhinal cortex in significant ways. It is fundamentally part of the hippocampal
complex and therefore involved in spatial memory and orientation. See Sect.
1.3.1.2 below.
6 S. Gadbois and C. Reeve
1.2.1.3 Orbitofrontal Cortex
Often labelled as the ‘secondary olfactory cortex’, this part of the cortex is known
to be important in decision making, as well as some cognitive processing of
reward, especially expectation and anticipation of rewards (Kringelbach and
Berridge 2009). It is therefore involved in the explicit processing of odours
(sniffing, as opposed to smelling).
1.2.2 Motor and Motivational Factors in Olfaction
The words ‘motor’, ‘motion’, ‘motivation’, and ‘emotion’ are etymologically
related, from movere, ‘‘to move’’ in Latin. They all have a neuropharmacological
connection as well: dopamine. This important excitatory brain neurotransmitter is
of great importance in the motor system at the cortical level (e.g., frontal lobes)
and subcortical level (the limbic system, the basal ganglia, and associated struc-
tures such as the ventral tegmental area, the substantia nigra, the nucleus ac-
cumbens, etc.). It is also implicated in activity in the olfactory system, including
the olfactory tubercle in the olfactory cortex (where it plays a role in the overall
‘reward’’ system of the brain; the pyriform cortex is rich in dopamine and
dopamine receptors), and finally the periglomerular cells in the olfactory bulb.
Cognitively, dopamine is associated with a broad range of cognitive functions,
including attentional processes, which are conceptually linkable to anticipatory
processes at the conative level (in fact, those constructs may be less conceptually
and practically distinguishable than currently believed).
Dopamine is involved motivational processes as well. As addiction to dopamine
agonists (amphetamines, cocaine) may suggest, the role of dopamine in modulating
the motivational system of addicts is remarkable. Interestingly, dopamine agonists
are known to increase motivation and anticipation, as well as olfactomotor behav-
iours and olfactory activity in the olfactory system. In fact, dopamine activity in the
lateral hypothalamus is associated with ‘‘stimulus-bound processes’’ (Panksepp
1998), such as exploratory olfactomotor behaviour—in other words, sniffing. Other
behaviours may be associated with this as well, such as mouthing, licking, whisking
(Deschênes et al. 2011) and more involved motor behaviours such as searching,
exploring, and manipulating behaviours. Incentive salience is a characteristic of
reward-predicting stimuli that define a system called the ‘‘WANTING system’’ by
Berridge and associates (Berridge 2001,2004). This system is discussed by Panksepp
as being the SEEKING system, and similar theories exist elsewhere: Gray’s
‘Behavioural Activation System’’ (Gray 1987) and Depue’s ‘‘Behavioural Facili-
tation System’’ (Depue 2000). Berridge’s theory is more integrated in our opinion as
it explains very well the balance between an arousal state of anticipation for the
reward (wanting or seeking the reward: what ethologists labelled appetitive behav-
iours) and the opposing system that takes over when the reward is acquired and being
consumed. Here Berridge and Panksepp agree on some of the details, including the
1 Canine Olfaction: Scent, Sign, and Situation 7
idea that those two systems are incompatible in the sense that if one is activated, the
other one is not (in normal conditions, and as Berridge would explain, addiction
would be an exception), and that what really motivate animals to do things, including
learning, is anticipating the reward, not consuming it: it is wanting it, not liking it.
Berridge explains well his integration of those two systems and their impact on
behaviour and learning (Berridge 2001; Berridge and Robinson 1998; Berridge et al.
2009). Some neo-behaviourist theories of conditioning postulated such quasi-cog-
nitive or cognitive factors in learning.
Neurocognitively and neuroconatively, ‘‘anticipations’’ and ‘‘expectations’
seem to be modulated by the dopaminergic system—although it is not the only
neurotransmitter system involved (see Table 1.1 showing how the LIKING system
taps into endorphins, in fact suppressing the WANTING system, therefore sup-
pressing behaviour). As a reminder, dopamine is the central neurotransmitter in the
WANTING/SEEKING system. It is important in olfaction and motor behaviour in
general, and therefore plays a role in olfactomotor behaviours, including the basic
behaviour of sniffing that is enjoying its own scientific literature in recent years (in
humans, rodents, and canines; see Mainland and Sobel 2006; Sobel et al. 1998;
Kepecs et al. 2005; Panksepp 1998). Sniffing is an exploratory behaviour that has
many important roles in olfaction: it actively participates in the input of the
olfactory stimulus, it can be modulated to account for different odorant concen-
trations, and it can modulate the pattern of neural activity (e.g., brain waves).
In relation to canines, in 1992, Arons and Shoemaker demonstrated that some
dog breeds have higher baseline levels of dopamine than others. Border collies and
huskies have high dopamine levels; livestock guarding dogs’ levels are lower. It is
difficult not to think about the role of dopamine in some human disorders when
thinking of dopamine and dog breeds that seem to follow the pattern. Think of
border collies. The terms ‘‘hyperactive’’, ‘‘obsessive’’, ‘‘compulsive’’, etc., are
frequently used to describe individuals of that breed. This basically translates into
the intriguing possibility that individual differences and breed differences in
baseline dopamine levels may have a direct impact on cognition, motivation,
learning, and overall olfactory behaviour and performance. It is interesting to note
that our most successful laboratory and field work dogs are Border collies. A
selection bias may be at play here since we recruit dogs volunteered by their
owners eager to find an occupation for their overactive pets who appear in need of
stimulation, but if we look at the retention of individuals (the ones that make the
cut for advanced laboratory or field training), Border collies dominate the roster.
As a general rule, they are good and hard workers: motivated, persistent, good
sniffers, and their attentional focus can be channelled (in most cases) very well.
Our ‘‘dopamine hypothesis’’ essentially highlig htsthe possibi litythat ‘‘software’’-
level characteristics (neurochemical and neural-level mechanisms and processes) are
more important than the often-touted ‘‘hardware’’ characteristics. For example, we
never had any luck with Bloodhounds and other hunting ‘‘scent dogs’’ mostly because
of motivational issues, resilience, ability to work long hours or consistently, and
overall performance and energy levels. By contrast, the ‘‘work ethics’’ of high-
dopamine breeds, like the Belgian Malinois, Jack Russell, and Parsons, is remarkable.
8 S. Gadbois and C. Reeve
Although we have not yet had the opportunity to work with these breeds, some field
biologists working with wildlife conservation canines believe Jack Russells are the
ultimate detector breed: Engeman et al. (1998) calls them ‘‘the unique detector
dogs’’. We know local dog trainers and handlers in the Canadian Maritime provinces
working in bed bug detection that would share that belief.
1.3 Between Nose, Brain, and Mind: Cognitive Processes
1.3.1 Neurocognitive Sub-Systems
The visual system has two pathways (or streams) of processing information from
the outside world (Schneider 1969). One is the WHAT system for object recog-
nition, and the other is the WHERE system for spatial vision and localization.
From an evolutionary perspective it is believed that those systems evolved in order
to make sense of the immediate threats and potential foraging opportunities
afforded to the animal. In this section, we argue that the olfactory system can be
conceptualized the same way. It may be too early to determine the neuroana-
tomical boundaries and localization of these subsystems (assuming it is even
relevant), but at least the processes involved can be identified. To those two main
systems, we will add one that may be of crucial importance to olfaction: HOW
MUCH. Table 1.2 summarizes the perspective we propose.
1.3.1.1 The WHAT System
The psychophysics literature makes a clear case for the distinction between detection,
discrimination, and identification. Those three processes are part of the ‘‘what’’
system. We will briefly describe the processes involved. Note that in terms of higher
Table 1.1 Theoretical relationships between anticipatory and reward systems of the brain
Appetitive behaviours Consummatory behaviours
Traditional system(s) SEEKING system (Panksepp)
Behavioural activation system (Gray)
Behavioural facilitation system
(Depue)
‘Reward system’’
Berridge’s perspective WANTING system LIKING system
Associated brain areas Hypothalamus, basal ganglia and
associated structures
Limbic system: Amygdala,
Hippocampus, Septum,
etc.
Associated behaviours Exploratory behaviours: foraging,
stimulus-bound sniffing, mouthing,
licking
‘Feeling good’’
Associated
neurotransmitters
Dopamine Endorphins
1 Canine Olfaction: Scent, Sign, and Situation 9
level (cognitive) processing, we have already identified the pyrifom cortex as an
important role player in the WHAT system.
Detection defines the identification of one stimulus (e.g., grapefruit oil) or
stimulus category (e.g., citrus essential oil) among background noise or interfer-
ence. Note that we recognize the importance of early stimulus generalization when
inferring categorical detection.
Discrimination defines the identification of one stimulus (e.g., grapefruit oil,
referred to as the S+ or positive stimulus) or stimulus category (citrus oils) as
contrasted to another often similar stimulus (e.g., orange oil, the S-or negative
stimulus) or another category (e.g., floral oils).
Identification is a process by which a more explicit knowledge of the stimulus is
made. In humans, for example, ‘‘naming’’ the stimulus would be a demonstration of
this level of discriminatory process. Matching-to-sample tasks attempt to get at that
level of investigation; that is, the hope is that matching a sample with a target among
many other choices is an indication that the animal explicitly ‘‘identifies’’ the target
as the ‘‘same as’’ the sample. By definition, identification is preceded by detection
and discrimination. We will use the laboratory technique of simultaneous matching-
to-sample as an example. Imagine a set of four exemplars: lavender, grapefruit,
sandalwood, and bergamot oils. Each of these odorants can be used as the sample to
be matched with one or more instances of the same odours. For example, if presented
with a lavender oil sample, a dog may be required to pick the matching sample in an
array (matrix, line-up, or any other arrangement) of two, three, or x number of
choices that can include any of the oils in the initial set and/or distractors. Another
example is same-or-different judgments: dogs are trained to investigate two odours
and simply indicate if the samples are the same or different (by pressing or poking a
paddle, for example). This specific type of learning, although at first glance simple
and elegant, has been found to be very difficult if notimpossible to acquire by dogs in
our lab. Colleagues in developmental psychology have pointed out to us that even
children have a hard time with matching-to-sample (non-matching-to-sample tasks
being often acquired more readily) and same-or-different judgment tasks (Diamond
et al. 1999, Diamond 2006; Overman 1990; Premack 1983).
1.3.1.2 The WHERE System
The localization of olfactory stimuli is crucial in the context of finding food and
mates, just to mention the most obvious. We have identified the entorhinal cortex
as being an integral part in olfactory processing in the context of spatial memory
Table 1.2 Olfactory neurocognitive systems and corresponding neuroanatomical centres
WHAT WHERE HOW MUCH
Detection Searching Scaling
Discrimination Trailing
Identification (e.g., matching) Tracking
Pyriform cortex Entorhinal cortex Olfactory bulb; cortical?
10 S. Gadbois and C. Reeve
and likely localization of odours. It should be no surprise that canids, as predators,
have been efficient at using their olfactory sense to survive and reproduce. What is
less obvious is how this works beyond the WHAT system. Assuming the canine
knows the target scent (can detect it from background interference, can discrim-
inate it from similar odours that may be less relevant, and can identify it in more
complex situations), the issue of finding it when no other sensory modality can
help (especially vision and audition) is less obvious. This is where laboratory
conditions fail to give a full sense of the complexity of the processes necessary to
‘find’’ target odours.
The ‘‘sniffer dog’’ literature often distinguishes between trailing and tracking
dogs. There is in fact a significant confusion between these two processes, and in
some ways the distinction may be somewhat artificial or irrelevant to brain and
behavioural organization. But since it is an accepted conceptualization and often
defines specific training methods, protocols, and even dogs, we will include the
nuance in the WHERE system category. Not unlike the processes in the WHAT
system, the WHERE system addresses an incremental level of complexity in terms
of processing the stimulus. In this case, the stimulus is entirely ‘‘in context’’: in a
dynamic environment, meaning that the animal needs to be in foraging mode and
move around. Our experience in lab conditions suggests that a motor involvement
in active searching involving rooting and burying to find an odour source may be
facilitating detection and identification, despite the added olfactory noise coming
from the substrate. Our hypothesis is consistent with data presented by Hall et al.
(2013). We are currently investigating this intriguing hypothesis further. This
factor may also explain the ‘‘field effect’’, that is, the often radical and counter-
intuitive loss of performance in dogs that experience field conditions after labo-
ratory training. Motor integration between basic locomotor functions and olfac-
tomotor functions may be crucial for the system to work efficiently. Therefore,
searching, trailing, and tracking are uncommon areas of research but promising
behaviours to study. This is particularly evident with some of our dogs in the scent
processing program at the Canid Behaviour Research Lab that are trained in the
lab and later transferred to the field. For half of our dogs, at minimum, it is almost
impossible to bring them back to work in lab conditions. They seem to have lost all
motivation for the low stimulation (and contamination) of the laboratory envi-
ronment (it is possible that the absence of cues associated with reward would be
the cause). Alternatively, dogs may simply not be stimulated enough cognitively—
something that we address often with Border collies that seem to need being
constantly challenged. Our discussion of neuroconative processes addresses the
potential reasons behind this phenomenon documented by other teams (e.g., Smith
et al. 2003) that we have labelled ‘‘field effect’’. It is one type of ‘‘motivational
collapse’’ (also our term) that is often reported by dog handlers and trainers
familiar with working dogs.
Searching The first step in localization, before the stimulus is acquired, is to
search. Searching requires the animal to have an identified target, and in applied
settings, it may require the dog to memorize biologically irrelevant stimuli (e.g.,
looking for drugs or explosives). The important dimension of this step is that the
1 Canine Olfaction: Scent, Sign, and Situation 11
stimulus has not yet been detected. The early stages of foraging behaviour are
essentially ‘‘searching’’ behaviours.
Trailing Trailing is often defined as searching, at least in the early stages, but
also may suggest that the stimulus is acquired, but not yet localized. In other
words, cues are detected that announce the presence of the target, but the exact
localization or path taken by the target is not yet identified (and may never be).
This process requires significant amount of ‘‘air scenting’’ or sampling the air, as
opposed to ‘‘ground scenting’’ or investigating the ground.
Tracking Tracking is much more specific and there is a consensus on the
definition of the term. In tracking, the target is acquired, and the path taken by the
moving target is also identified and followed (with different levels of spatial
accuracy).
1.3.1.3 The HOW MUCH System
There are at least two situations, natural and artificial, that may require the dog to
assess the quantity of molecules present in the environment. Volatiles will be
distributed according to a specific gradient influenced by contextual conditions
(temperature, humidity, barometric pressure, and most importantly, air movements
such as drafts and wind), not to mention the distance between the dog and the
target (and obviously all this in relation to the actual saliency of the stimulus).
Much of this category is akin to the ‘‘scaling’’ process known in psychophysics.
Training and experimental conditions may require a dog to identify a threshold
and, for instance, give a positive response if the stimulus is above threshold and
give a negative response or no response if the stimulus is below that threshold. An
example would be a dog trained to identify Varroa destructor (parasitic mites) and
Nosema apis (fungus) in beehives. Both are potentially important factors in colony
collapse disorder (CCD). Most beehives in North America are infected with some
level of Varroa and Nosema, but the applied issue would be to train a dog to
identify hives infected beyond a specific threshold, highlighting the need for an
immediate intervention.
Interestingly, it is unclear whether the HOW MUCH system would actually be
able to discriminate within the actual volumetric quantity of a given stimulus (of
biological significance or having a primary incentive value—e.g., food). A study
by Horowitz et al. (2013) suggests that pet dogs may not differentiate between low
and high quantities of food based on olfactory cues alone. It is possible that a larger
differential between small and large amounts would have resulted in more sig-
nificant results (in terms of physical or chemical volatility), or perhaps the dif-
ferential incentive value of the stimuli was minimal enough to keep them
indifferent in their choice of food source.
The HOW MUCH system likely serves a function in processing gradients—in
other words, helping the dog to determine the direction of a source of volatiles.
12 S. Gadbois and C. Reeve
This specific question has been discussed in the context of directionality of tracks.
The basic question is as simple as ‘‘Do dogs know if they backtrack or forward-
track a target?’’, and as a corollary, ‘‘Can they make a mistake?’’. It seems logical
that, in order to survive, wolves would have had to ‘‘know’’ where their prey was
going, as backtracking would be counterproductive and maladaptive. A debate has
been ongoing regarding this issue with dogs (Thesen et al. 1993; Steen and Wilson
1990; Wells and Hepper 2003; Hepper and Wells 2005). Interestingly, Wells and
Hepper (2003) found that dogs were not good at ‘‘detecting’’ direction. Only
36.3 % of the dogs studied could do this consistently. Steen and Wilson (1990)
suggested that the training (read ‘‘learning’’ for ethological, non-artificial contexts)
may be of importance in determining if dogs track in the right direction. Thesen
et al. (1993) identified three stages in tracking behaviour: a ‘searching’’ phase
(before the stimulus or target is acquired, as described above), a ‘‘deciding’’ phase
(when the dog determines the directionality of the moving target), and a ‘‘track-
ing’’ phase. They also found the dogs to be more accurate and consistent than those
in the study by Wells and Hepper (2003). Note that this literature and perspective
on the HOW MUCH system may link it directly to the WHERE system. It may
even suggest that it is a sub-system of the WHERE system or simply needs to be
fully merged with it.
Most of the traditional fundamental research on canine olfaction and applied
research with sniffer dogs has focused on the WHAT system (e.g., odour dis-
criminations) and the HOW MUCH system focusing on detection thresholds, in
other words, more traditional psychophysics experiments (see Helton 2009a,band
Lit 2009 for reviews; see also the work of the Auburn University College of
Veterinary Medicine group, e.g., Furton and Myers 2001). Research on tracking
per se is at its infancy, mostly because of the methodological constraints imposed
by moving subjects during searching, trailing, and tracking but also because of the
challenges presented by field work.
1.4 Methodology: Psychophysics, Olfactory Learning,
and Cognition
Section 1.3 presented our three-system perspective on the sensory and cognitive
processes that work in synergy to process information. Now we move on to the
methods to investigate olfactory capacity. Studying olfaction in animals has been a
challenge in that what are salient odours for most non-human mammals (with
exceptions, e.g., cetaceans) are for humans ‘‘invisible’’ and often undetectable
stimuli. Experimental psychology has provided effective tools to study sensory
processes in animals using mostly operant methods (Blough 1966; Blough and
Blough 1977). Quantitative tools in human psychophysics have also contributed to
the application of Signal Detection Theory (SDT) to olfactory stimuli, either in
1 Canine Olfaction: Scent, Sign, and Situation 13
detection tasks (one stimulus in a noisy environment) or discrimination and
identification tasks (discriminating between two stimuli). While we are not cov-
ering it here, the basics of SDT for canine olfactory processing are discussed in
Helton (2009a,b). McNicol (2005) is a short yet useful resource to cover the basics
of SDT and MacMillan and Creelman (2005) is a comprehensive resource to cover
advanced applications of SDT. The latter includes its use in designs such as two-
alternative forced choice designs (2AFC) and multiple alternative forced choice
(mAFC), same-different, matching-to-sample, and oddity design (triangular
method) (Lit 2009). Although written with humans and mostly visual stimuli in
mind, olfactory stimuli can be used with these approaches. It is also worth men-
tioning that although SDT is usually used as a parametric tool, a non-parametric
version of SDT also exists (Pastore et al. 2003).
1.4.1 Habituation-Dishabituation
Slotnick and Schellinck (2002) also review methodologies used with rodents,
including an interesting non-operant technique called ‘‘habituation-dishabitua-
tion’’. The method is often used in our lab before training dogs on a specific scent
when we are in the early stages of a project. As habituation (and dishabituation)
are non-associative, ‘‘simple’’ forms of learning, no training is required. We use
this technique to test the ability of dogs to naturally detect two given odours. For
example, in 2009, our laboratory started a project with Parks Canada that required
sniffer dogs to find and potentially track Eastern Ribbon Snakes (Thamnophis
sauritus sauritus), a species-at-risk in Nova Scotia. One of our worries was that
Common Garter Snakes (Thamnophis sirtalis) are very common in the same
habitat and areas where the dogs were going to work. Both species are of the same
genus and to a human nose, smell quite the same. The procedure typically includes
five trials. The first four are the habituation phase, when the dogs are exposed to
the target scent for five minutes. During that time, the duration of sniffing (sniffing
time) is recorded. The dogs are given a break of fifteen minutes between each
exposure. Typically, by trial four, the sniffing time has been reduced dramatically.
On trial five, the new scent (Common Garter Snake) is introduced. This is the
dishabituation phase. If the dogs perceive the smell as different, it is assumed that
the sniffing time will increase dramatically from trial four because of the novelty
of the smell (Gheusi et al. 1997; Vaché et al. 2001). It is expected that the sniffing
time would approach the sniffing time of the first trial. In our case (Gadbois et al.
in prep), all dogs increased their sniffing time significantly, more than doubling the
sniffing time for trial one (see the Fig. 1.1). This can be interpreted as a strong
novelty effect, suggesting dogs can naturally discriminate the two smells. A
control condition—a cotton ball without the smell—is always added within each
trial. This immediate, within-trial control allows the experimenter to determine if
the dog is sensing the target odours.
14 S. Gadbois and C. Reeve
1.4.2 Errorless Discrimination Training
Our lab has also worked on adapting Errorless Discrimination Training (EDT)
(Terrace 1963a,1963b,1964,1966) to olfactory discriminations with some success.
Using the same example as above (training the Ribbon Snakes as the S+ or target
scent versus Common Garter Snakes as S-or non-target scent) we use an adaptation
of EDT. Terrace developed the procedure for colour (wavelength) discrimination in
pigeons. As far as we know, EDT has never been applied to scent detection and
discrimination. It differs from traditional discrimination training in a number of ways:
There is no peak shift: i.e., gradual biases in discriminations away from the
target stimulus do not occur.
There is a large reduction of errors compared to traditional discrimination
learning (*45 times fewer errors) (Terrace 1963a,b).
EDT is based on excitatory conditioning only. In other words, mistakes (errors)
are not required for learning. Thus, there are no negative emotions during
training (e.g., frustration, helplessness, stress, anxiety, etc.), so the training does
not become aversive.
In traditional discrimination training methods (TDT henceforth), a target odour
is presented (S+) and a non-target odour is presented (S-). Presentations of S+ and
S-can be simultaneous or sequential. Responses to S+ are always rewarded, at
least initially, and responses to S-are never rewarded. EDT’s procedure is
simpler, yet somewhat counterintuitive for most people familiar with TDT: ini-
tially, only S+ is presented, and a response specific to S+ (e.g., ‘‘sit’’) is rewarded.
Soon after the beginning of the training, S-is presented alongside S+ but in very
low concentrations. The concentration of S-is gradually and slowly increased
Fig. 1.1 Data showing Zyla’s (expert sniffer dog) habituation/dishabituation test comparing
Eastern Ribbon Snake scent to Common Garter Snake scent (Gadbois et al. in prep)
1 Canine Olfaction: Scent, Sign, and Situation 15
until it is at the same level as S+. This process is called ‘fading-in’’. Theoretically,
dogs learn to ignore S-, and to only responded to S+. As mentioned above, errors
made by animals with this type of training are radically reduced compared to TDT.
The main drawback of this technique is that transfer of learning (during re-
training) may be longer and more difficult for the dog. EDT dogs are more likely to
become a ‘‘one-smell-dog’’ than TDT dogs, but they are very accurate for that one
smell and the learned behaviour is very resistant to extinction.
The problem with EDT is to find a way to deliver the stimuli with a fading-in
procedure in place. Olfactometers could be used, but we decided to explore a
simpler and cheaper method. Our training used the Ribbon Snake odour (S+: a swab
of a live animal). Part of the training takes place with the scent of other snake
species or distractor odours (S-: food, dog smell, Common Garter Snake odour). A
small rectangular aquarium is used to present the odours. Each sample (S+ and S-)
sits at the bottom of the aquarium. Two containers (baby food jars or stainless steel
spice containers) are placed in the aquarium, above water level, one containing the
S+ (Ribbon Snake smell), the other the S-(e.g., Garter Snake smell). The location
(left or right) of the S+ is randomly determined between trials. The Ribbon Snake
sample is placed directly in the well, but the S-smell is contained in an ice cube
that gradually melts, increasing ever so gradually the saliency of the smell. Since
we do not control the presentation of the smell in discrete presentations, the dog is
taken in and out of the testing room. In our case, ice cubes would take between 4
and 5 h to melt completely. Dogs are then invited to visit the experimental room
and are rewarded (e.g. with play, food, or praise) for any attention paid to the S+ and
simply ignored for any time spent sniffing the S-. The saliency of the S-(and the
S+) can be increased towards the end of the exposures or accelerated overall to
reduce the length of the session by using a hot plate or other heating device. The
aquarium has a cover and multiple small holes allowing the scent to diffuse out of it.
Time spent smelling the target side of the box is measured (and as mentioned above,
counterbalanced between trials to avoid lateral preferences and learning) and
rewarded. Within 3 to 5 h (or depending on the volume of the frozen sample), the
dog typically sniffs only the S+ and ignores the S-. This type of learning can be
transferred and generalized to procedures requiring the shaping of a more explicit
response (e.g., nose poking and holding for a few seconds at the target stimulus).
Our experience with this technique so far has been promising, although we often
run into the problem of having a dog perfectly able to distinguish between the S+
and S-, but unable to then quickly learn a desired operant response to signal the
presence of a target (unpublished data).
1.4.3 Line-Ups: Memory Load Issues in Scent Processing
The line-up is another very common method in olfactory discrimination and
identification. It is traditionally used by the police forces and the military and has
become common in many modern applications for training as well as experimental
16 S. Gadbois and C. Reeve
and biomedical trials (e.g., cancer detection). For example, Schoon and Haak
(2002) wrote what is often considered the standard for forensic work with canines.
Their use of line-ups in the context of a matching-to-sample problem has many
potential applications. In their case, they describe the training of dogs to match
objects found at a crime scene with a potential perpetrator. At first glance, the
concept seems to make sense as dogs act here as witnesses. Following discussions
with a colleague at Dalhousie University, Dr. John Christie, a cognitive psy-
chologist, we realized that the dogs may be ‘‘expert witnesses’’, but they are not
comparable to expert witnesses participating in a photo or person line-up as they
were not at the crime scene. In other words, we are not testing their memory of a
past event, but we are instead testing their sensory-perceptual matching of the
odour of a person, and the odour of an object with which they were in contact. So
the question then becomes: Why make this task a memory task?
In the early 1970s, a former mentor of one of us (SG), Werner Honig, intro-
duced into animal learning, from human cognitive psychology, the concept of
working memory. Honig was by training a traditional operant conditioning
researcher who edited seminal books in the field (Honig 1966; Honig and Staddon
1977). Growing increasingly dissatisfied with a ‘‘pure’’, traditional approach to
instrumental conditioning, Honig became receptive to the memory literature in
humans and applied many of the theoretical and conceptual foundations of human
cognition to animal learning (he contributed a number of edited books on the topic,
e.g., Honig and James 1971; Hulse et al. 1978; Honig and Fetterman 1992, and
many articles addressing working memory in pigeons: Honig 1978,1981,1984).
Although his work was never applied to canines and was largely restricted to
pigeons (a traditional model in classical behaviourism), the concept of working
memory in animals is now largely accepted in animal cognition textbooks. The
basic idea is that information presented to animals may stay in memory for a short
period of time, for the time necessary to complete a task. Working memory, as a
specific type of short term memory, is prone to interference, and tends to fade
rapidly when not in use.
Let us go back to the line-up: Remember that a line-up is a special case of
matching-to-sample—the sample being the ‘‘cue’’ given to the dog, i.e., what to
find. The general procedure in a formal test of ‘‘expert canine testimony’ would be
to present the dog with the smell of a suspect, or sample (e.g., sweat sample on a
cotton ball) and ask the dog to walk a line-up of containers containing objects, one
of which could be the potential target. Conversely, the object found at the crime
scene could be the sample, and a line-up of sweat samples from different people
(including the suspect) could be available for investigation. Note that the most
standard procedure includes six containers with samples to sniff: one target and
five foils or distractors. The position of the target is typically randomly determined
by throwing a die. Note also that dogs are typically trained to not respond to blank
line-ups, so they should know that a ‘‘no target’’ condition is possible, thus
reducing false alarms. In addition, they should be trained to identify two instances
of the same target in the same line-up to encourage them to complete the sampling
of the line-up even if the target is in an early position.
1 Canine Olfaction: Scent, Sign, and Situation 17
The working memory issue is easy to miss. Taking into consideration that the
dog is not an actual witness of a crime trying to remember information about a
crime scene, it is puzzling that we would increase interference with such a pro-
cedure. Added interference comes from the information in working memory after
the inspection of each station containing a sample to inspect. Since this is done
sequentially in one scan of all six containers, you can imagine that by the time the
dog inspects the fifth and sixth samples, it may not remember the characteristics of
the cue or initial sample.
We decided to put this hypothesis to the test with a mini-experiment with two
dogs that were considered experts at line-ups and were of equal overall perfor-
mance (80–100 %, averaging 90 %). Dogs were trained on diluted essential oils
and in this case, we identified a specific target (lavender oil) to find in the line-up.
We measured the accuracy of the dogs based on the position of the target in the
line-up. The position of the target was randomly determined by throwing a die
over 200 trials. As expected, if the target was early or close in the line-up
(positions 1 and 2), accuracy was high ([90 %). But if the target was late or far in
the line-up (positions 5 and 6), accuracy dropped significantly (\40 %)
(Table 1.3). To go back to the forensic example with canine ‘expert witnesses’’,
imagine now that the target associated with a perpetrator has been randomly
assigned to position 6. The dogs have less than a 15 % chance of making an
accurate match. Note also that we are not working with degraded stimuli. In fact,
even if significantly diluted, essential oils are so strong and salient that we stopped
using them a few years ago in favour of tea and other infusions in the early training
phases of detection-, discrimination, or matching dogs.
After realizing this pattern was common when using line-ups, and since we are
not interested in mnemonic performance but rather psychophysical accuracy at the
sensory and perceptual level, we started using simpler tasks with a reduced number
of potential choices. Simplicity is our best ally in those situations. If possible,
using simple designs and procedures (e.g., go/no-go, a single-scent task, or 2AFC
or 3AFC if more than one operant response is necessary) is preferable. In other
words, as discussed in Lit (2009), reducing the cognitive demand of the task will
accelerate learning and likely increase performance. By analogy, in the case of the
line-up, making sure that the matching-to-sample is simultaneous (i.e., the options
are made available immediately, as opposed to a delayed matching-to-sample),
and a small number of options is offered, would help performance. In other words,
the spatial and temporal contiguities should be such that the task does not tax
memory processing resources, but only sensory-perceptual processing resources.
Table 1.3 Sequential position of target and performance of two expert detection dogs in a line-
up procedure
Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Dog 1 97.7 % 93.7 % 90.6 % 82.7 % 28.5 % 10.5 %
Dog 2 97.7 % 90.6 % 93.7 % 79.3 % 35.7 % 13.1 %
# trials 45 32 32 29 28 38
18 S. Gadbois and C. Reeve
1.4.4 Remote Scenting: Attentional Load Issues
in Scent Processing
Sometimes the presence of the dog ‘‘on site’’, especially in applied settings, is not
desirable or even safe. Field conditions are sometimes too hazardous for the dogs
to be present (land mine detection dogs can get injured or killed in the field),
sample collection of rare species sign with wildlife conservation canines may be
infrequent and occur over large expanses of terrain or in remote and difficult to
access areas (collecting scats and identifying potential latrine sites for Eastern
Cougars), or the dog is simply not able to easily work on premises (e.g., in clinics
and hospitals for diagnostic detection or in interactions with patients). In those
examples, practical reasons would normally exclude dogs from being part of the
detection, searching, tracking, etc. Remote-scenting protocols were developed
with these cases in mind, and an important historical first case was the develop-
ment of Remote Explosive Scent Tracing (REST) with land mine detection in
mind (Fjellanger et al. 2002; McLean et al. 2003; see Helton 2009a,bfor a short
review). One other clear advantage of this method addresses the issue of attention
and an attempt with remote scenting to reduce attention demands by having the
dogs work in controlled, consistent, and familiar indoor conditions. Microclimatic
and micrometeorological conditions (temperature, humidity, air movements) can
be controlled and delivery methods can be designed to optimize scent perception.
1.4.5 Ethological Approaches and Future Lines
of Investigation
The study of canine olfactory psychophysics and learning has certainly benefited
from the tradition of behaviourism and behaviour analysis, including applied
behaviour analysis. Indeed, applied research with sniffer dogs seems to be the main
impetus for funding and research opportunities in olfactory cognition, especially if
an experimental approach is favoured. Unfortunately, there is an immense gap in
the study of olfaction in canines: The true ethological field approach when looking
into mid- to long-range tracking and trailing processes. Potential approaches to this
may include the use of optical tracking methods (as used in path integration in
canines by Séguinot et al. 1998) and GPS/GIS technology, when the technology
becomes less expensive and more accessible.
A clear understanding and deep knowledge of animal learning and cognition is
without a doubt useful to the development of experimental programs seeking to
understand the fundamental and applied dimensions of canine olfaction.
1 Canine Olfaction: Scent, Sign, and Situation 19
1.5 Medical Detection and Assistance Canines: Cancer,
Diabetes, and Epilepsy
The challenges of applied research are numerous. Although not our primary
applied research area, we will present a quick review of a fascinating emergent
area of biomedical research involving the training of dogs for detection of and
assistance for medical problems.
The relationship between owner and pet can be a very fulfilling and rewarding
experience. Recent advances in the field of health research suggest that dogs may
soon be more than just our pets and that the nature of our relationship with them
could change drastically.
1.5.1 Cancer Detection
The first evidence of a dog’s ability to detect disease came from the well-known
report by Williams and Pembroke (1989) in the Lancet, in which a woman sought
medical attention after her dog persistently sniffed a mole on her leg. Upon clinical
examination, the spot was discovered to be malignant melanoma. What was it the
dog smelled that interested it so much? Advances in technology suggest that
diseases such as cancer likely have a ‘‘signature scent’’, characterized by the
volatile organic compounds (VOCs) being released (Szulejko et al. 2010).
In the 1970s, Linus Pauling et al. (1971) found 200 VOCs in exhaled human
breath. Since then, more than 3400 VOCs have been documented in human breath
(Phillips et al. 1999). VOCs released in the breath, urine, and tissues may provide a
window into biological processes, with certain biological markers indicating
specific medical conditions (Bijland et al. 2013; Buszewski et al. 2012; Miekish
et al. 2004; Szulejko et al. 2010).
In 1999, Phillips et al. obtained breath samples from 50 healthy individuals and
analyzed the biological components of the samples using an analytical technique
called Gas Chromatography—Mass Spectrometry. The analysis revealed a total of
over 3400 different VOCs. Importantly though, each individual breath sample was
found to have an average of 204.2 VOCs, and only 27 VOCs were found to be
present in every sample. This demonstrates a huge level of variability between
individuals’ breath samples. Such variation is likely due to differences in diet,
drugs, medication, metabolism, and health status, to name a few factors (Phillips
et al. 1999).
Can dogs detect differences in the VOCs being emitted by their owners? Can
they even smell the VOCs? Evidence would suggest that this is the case. The
burgeoning science of canine olfaction has elucidated just how sensitive the nose
of a dog is. For example, using an olfactometer, Waggoner et al. (1998) showed
that dogs were able to detect a target odour present in one part per billion in the
presence of a distracting odour at a concentration of twenty parts per million, and
20 S. Gadbois and C. Reeve
Pearsall and Verbruggen (1982) reported that dogs can smell some odours at one
part per trillion.
The ability of dogs to sniff out melanoma was empirically tested by Pickel et al.
(2004). Using two well-trained dogs, one of which was experienced in cancer
detection, Pickel et al. first confirmed the dogs’ ability to detect melanoma tissue
using a variety of search tasks. Researchers then placed between 8 and 30 adhesive
bandages on human participants, one of which covered the site of the cancerous
tissue. The first dog successfully identified the correct bandage on 6 out of 7
patients, while the second dog was successful in sniffing out melanoma on 3 out of
4 patients.
Horvath et al. (2008) showed that a naive Riesenschnauzer (neither the dog’s
previous level of training, or experience in olfactory detection was men-
tioned) was able to detect ovarian cancer from cancerous ovary tissues with 100 %
sensitivity and 97.5 % specificity. Moreover, control tissues in this study included
abdominal fat, muscle, small bowel tissue, healthy postmenopausal ovarian tissue,
and some tissues from an area just adjacent to the tumor. Given the impressive
results, this study serves as excellent evidence that cancerous cells have a distinct
odor that is reportedly detectable by dogs.
Detection of cancer in urine and fecal samples has yielded more conflicting
results. Willis et al. (2004) reported that after a seven month training period, dogs
with no prior experience could detect bladder cancer in urine samples at 41 %
accuracy (as compared to 14 % expected by chance). Using urine samples to test
dogs’ ability to detect breast or prostate cancer, Gordon et al. (2008) had ten dogs
trained by professional dog trainers. The reported successful detection rate for
breast cancer was 22 % and only 18 % for prostate cancer.
More promisingly, Cornu et al. (2011) were able to train a Belgian Malinois
with no prior experience to detect prostate cancer from urine samples with a
sensitivity and specificity of 91 % in a period of 24 months. Furthermore, Sonoda
et al. (2011) tested a trained cancer-detection Labrador retriever’s ability to detect
colorectal cancer in both breath and watery stool samples from patients with
different stages of colorectal cancer. Control samples were obtained from patients
with other colorectal conditions such as chronic inflammatory disease and various
forms of colitis. The dog was able to detect colorectal cancer in the breath samples
with 91 % sensitivity and 99 % specificity, and in the watery stool samples with
97 % sensitivity and 99 % specificity.
Still, based on the available literature, it would appear that testing the detection
of cancer by dogs is more consistently successful with the use of breath samples.
McCulloch et al. (2006) used a three-phase training program that spanned only a
couple of weeks to train naive sniffing dogs to detect lung and breast cancer from
breath samples. Testing revealed the dogs’ ability to detect breast cancer with a
specificity of 98 and 88 % sensitivity, and lung cancer with 99 % specificity and
sensitivity.
Ehmann et al. (2012) had four family dogs trained by professional dog trainers
to detect lung cancer from breath samples (no mention of length of training). In
this study, sample controls were from patients with a non-malignant lung disease.
1 Canine Olfaction: Scent, Sign, and Situation 21
Here, dogs were reported to successfully detect lung cancer at a sensitivity of 72 %
and specificity of 94 %.
Empirical studies of the ability of dogs to detect cancer are still in their infancy.
Inconsistent findings are likely the result of differing training programs and sample
collection techniques (Moser and McCulloch 2010), as well as breed-specific
behavioural profiles as suggested by our dopamine hypothesis (Sect. 1.2). How-
ever, given the reported ability of dogs to successfully detect cancer despite
potential confounds and biologically comparable control stimuli (Ehmann et al.
2012; Horvath et al. 2008), these studies provide an extremely promising and
intriguing area of study that warrants much further investigation.
1.5.2 Diabetes Detection
Anecdotal evidence suggests that dogs (and cats) may be able to prevent health
complications in individuals with insulin-dependent diabetes by signaling
impending hypoglycemic events in their owners (Chen et al. 2000; Wells et al.
2008,2011). In a series of case studies, anecdotal evidence from individuals with
diabetes suggests that their dogs were aware of fluctuations in their blood sugar
levels before they experienced symptoms. Furthermore, some dogs woke owners
during the night, and one even signaled through a closed bedroom door (Chen
et al. 2000).
Although there are companies claiming to train hypoglycemia detection dogs
(e.g., CARES, Canine Assistance Rehabilitation Education and Services), there are
currently no empirical studies confirming this ability in dogs, and there is only
speculation as to what the dog is detecting before a hypoglycemic event.
Researchers hypothesize dogs may be using olfactory cues such as a change in the
chemical composition of their owners’ sweat (sweating is a common symptom of
hypoglycemia), or that signaling dogs are acutely aware of the behavioural
changes accompanying hypoglycemia in their owners (Wells et al. 2008).
In an attempt to elucidate the mechanism with which dogs may detect hypo-
glycemia, our Canid Behaviour Research Team at Dalhousie University has
teamed-up with colleagues at the IWK Health Centre in Halifax, Drs. Elizabeth
Cummings and Elizabeth McLaughlin. We began in early 2013 a series of projects
to examine potential biochemical routes of detection. Our dogs are selected based
on motivation levels and their performance in detecting low-saliency stimuli.
Those dogs that are selected are tested on their ability to detect glycemic changes
in breath, sweat, and saliva samples from individuals with Type 1 diabetes. The
dogs are presented with a forced choice task that requires them to match hypo-
glycemic samples with hypoglycemic samples, in the presence of normoglycemic
samples from the same individual. Successful matching would indicate the dog’s
ability to discriminate between glycemic levels from samples in vitro, in the
absence of the actual patient. Although the project is still very young, preliminary
results are inconclusive. In the future, we would like to test the hypothesis that
22 S. Gadbois and C. Reeve
what dogs may be detecting in their owner is actually a myriad of physiological
and behavioural changes, therefore detection would only be possible in vivo. If this
is found to be the case, we would also like to test the idea that hypoglycemia-
detection dogs are responding to a generalized stress response. Anecdotal evidence
may suggest that trained hypoglycemia detection dogs respond to a variety of
biologically stressful events (e.g., asthma attack) in their owners and others and
this may be an indication that physiological stress markers (e.g., increase in
adrenalin, cortisol levels, etc.) may be detected, and not glycemic VOCs per se
(e.g., personal communication, Sarah Holbert of CARES, March 2013).
1.5.3 Seizure Alert Dogs
Until recently, only anecdotal reports of dogs signalling oncoming seizures in their
owners existed. However, recent empirical evidence has shown that dogs can
indeed detect seizures and can be trained to do so reliably (Kirton et al. 2008, and
for reviews see Brown and Goldstein 2011; Dalziel et al. 2003).
Strong et al. (1999) successfully trained six dogs to anticipate and signal an
impending seizure in a family member. Following a training period of six months,
the dogs learned to associate seizures with pleasurable events and consistently
signalled 15–45 min before a seizure. An unexpected result was that owners
reported a reduction in the frequency of seizures. Therefore, in 2002, Strong et al.
examined this directly by following epileptic patients 24 weeks after acquiring a
trained seizure detection dog. As reported by patients in the 1999 study, a
reduction in the frequency of seizures in almost all patients was observed (9 out of
10 patients, mean reduction of 43 %). Given that seizures are often preceded by
anxiety (Betts 1981) and that owners of seizure detection dogs have reported
increases in well-being (Kersting et al. 2009), it is possible that owning a trained
seizure detection dog provides feelings of comfort and safety (as discussed above),
thereby reducing anxiety and as a result, seizure frequency.
As with hypoglycemia detection dogs, it is not known what signals from the
owner alert the dog before a seizure. However, in this case, researchers appear
confident that trained dogs are recognizing and responding to minute changes in
the behaviour of their owner (Brown and Strong 2001), but detection of physio-
logical changes cannot be ruled out without further investigation (Wells 2007).
1.5.4 Where to Go From Here?
Canine detection of disease and the use of dogs as assistance dogs is an extremely
intriguing field. Evidence suggests that dogs can be trained to detect different
forms of cancer using olfactory cues from multiple biological channels. Testing
the VOCs in human biological samples provides an interesting alternative to
1 Canine Olfaction: Scent, Sign, and Situation 23
current screening methods for cancer. Depending on the cancer being tested for,
current screening techniques can be expensive, inaccurate, increase exposure to
radiation, and can result in unnecessary biopsies (Jett 2005; Gotzche and Nielsen
2006). The ability of dogs to detect impending hypoglycemic events in diabetic
owners is a phenomenon that merits further study. Based on the successful training
of seizure detection dogs, there is reason to believe that the validity of hypogly-
cemia-detection dogs may be empirically confirmed in the near future. Taken
together, the literature presented here suggests that in the future we will see dogs
not only as human’s best friend, but as our partners in health care, providing
detection of, assistance for, and treatment of disease.
1.6 Human-Canine Sensory Symbiosis and Appeal
for a Renewed (Situated) Science of Canine Olfaction
Much of our applied work at the Canid Behaviour Research Lab at Dalhousie
University is based on wild canid research and the use of sniffer dogs as ‘‘wildlife
conservation canines’’, helping us find our target species (e.g., coyotes, various
species-at-risk, or invasive species) in unobtrusive and non-invasive ways.
Although one of us (SG) has been using dogs in this capacity since the early 1990s,
it was not until a student (Flannery and Gadbois, unpublished manuscript) decided
to write a literature review on the topic that we realized the potential of this
association between humans as field researchers and dogs as research assistants.
As Hewes (1994) discusses, the symbiosis between humans and wolves or early
dogs may have been a question of survival, and the complementarity of our sen-
sory ecologies—visual humans, olfactory wolves—may have been the start of a
remarkable (mutualistic) symbiosis. This never became more obvious to me than
when a few years ago, in the scenic and majestic scenery of the Cape Breton
Highlands in Nova Scotia, we were looking for a pack of coyotes and a suspected
moose carcass site. Our sniffer dog Zyla was air scenting to localize the coyotes,
pulling in one direction, and ravens were converging in a slightly different
direction (towards, we realized later, the moose carcass). I could not stop thinking
about how this all made sense. Early humans would have relied on scavengers and
predators to locate food, and would have quickly realized that the keen sense of
smell of wolves was an asset.
As suggested and highlighted in our discussion of the biomedical (and com-
panionship, when assistance complements the detection work) applications of
canine olfaction, we are only at the beginning of the realization of the amazing
potential this partnership can offer.
Acknowledgements Sincere thanks to Alexandra Horowitz for inviting us to participate in this
exciting project. Thank you to all the dogs and their owners that participated in our research over
the years, the many hundreds of volunteers working in the lab, Honours and graduate students
(for a full list, see http://gadbois.org/simon/team.html). Simon Gadbois wants to take the
24 S. Gadbois and C. Reeve
opportunity to thank the many mentors who inspired him over the years, ‘‘in order of appear-
ance’’: Louis Gadbois, Ward O’Neill, Marvin Krank, Werner Honig, Vincent LoLordo, John
Fentress, Peter McLeod, William Moger, and Fred Harrington. You have no idea how much you
all contributed to shape and focus that mind of mine and force it to always want to synthesize and
keep an open mind, and like coyotes, be happy to be a generalist.
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1 Canine Olfaction: Scent, Sign, and Situation 29
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Olfaction in dogs is crucial for gathering important information about the environment, recognizing individuals, making decisions, and learning. It is far more specialized and sensitive than humans’ sense of smell. Using the strength of dogs’ sense of smell, humans work with dogs for the recognition of different odors, with a precision far exceeding the analytical capabilities of most modern instruments. Due to their extremely sensitive sense of smell, dogs could be used as modern, super-sensitive mobile area scanners, detecting specific chemical signals in real time in various environments outside the laboratory, and then tracking the odor of dynamic targets to their source, also in crowded places. Recent studies show that dogs can detect not only specific scents of drugs or explosives, but also changes in emotions as well as in human cell metabolism during various illnesses, including COVID-19 infection. Here, we provide an overview of canine olfaction, discussing aspects connected with anatomy, physiology, behavioral aspects of sniffing, and factors influencing the olfactory abilities of the domestic dog (Canis familiaris).
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Comprehensive Overview of Advances in Olfaction The common belief is that human smell perception is much reduced compared with other mammals, so that whatever abilities are uncovered and investigated in animal research would have little significance for humans. However, new evidence from a variety of sources indicates this traditional view is likely overly simplistic. The Neurobiology of Olfaction provides a thorough analysis of the state-of-the-science in olfactory knowledge and research, reflecting the growing interest in the field. Authors from some of the most respected laboratories in the world explore various aspects of olfaction, including genetics, behavior, olfactory systems, odorant receptors, odor coding, and cortical activity. Until recently, almost all animal research in olfaction was carried out on orthonasal olfaction (inhalation). It is only in recent years, especially in human flavor research, that evidence has begun to be obtained regarding the importance of retronasal olfaction (exhalation). These studies are beginning to demonstrate that retronasal smell plays a large role to play in human behavior. Highlighting common principles among various species including humans, insects, Xenopus laevis (African frog), and Caenorhabditis elegans (nematodes) this highly interdisciplinary book contains chapters about the most recent discoveries in odor coding from the olfactory epithelium to cortical centers. It also covers neurogenesis in the olfactory epithelium and olfactory bulb. Each subject-specific chapter is written by a top researcher in the field and provides an extensive list of reviews and original articles for students and scientists interested in further readings.
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Dogs have been trained to perform a diverse set of detection tasks. They have been trained to detect estrus in dairy cows, cancer, contamination in aquaculture tank water, compact discs and DVDs, invasive species, accelerants, explosives, narcotics, insect infestations, microbial growth, wood rot, gas leaks, toxins, and scat of a wide range of species. Olfaction in dogs (see Chapter 8 by Goldblatt, Gazit, and Terkel) and other vertebrates involves the action of chemically sensitive sensory cells located in the olfactory epithelium in the nasal cavity
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Since Canis lupus familiaris first shared a fire with man more than 15,000 years ago, dogs have been trusted and valued coworkers. Yet the relatively new field of canine ergonomics is just beginning to unravel the secrets of this collaboration. As with many new fields, the literature on working dogs is scattered across several non-overlapping disciplines from forensics and the life sciences to medicine, security, and wildlife biology. Canine Ergonomics: The Science of Working Dogs draws together related research from different fields into an interdisciplinary resource of science-based information. Providing a complete overview, from physiology to cognition, this is the first book to discuss working dogs from a scientific perspective. It covers a wide range of current and potential tasks, explores ergonomic and cognitive aspects of these tasks, and covers personality traits and behavioral assessments of working dogs. A quick look at the chapters, contributed by experts from across the globe and across the multidisciplinary spectrum, illustrates the breadth and depth of information available in this book. Traditionally, information concerning working dogs is mostly hearsay, with the exchange of information informal at best and non-existent at worst. Most books available are too general in coverage or conversely, too specific. They explain how to train a service dog or train a dog to track, based on training lore rather than empirical methods verified with rigorous scientific standards. This book, drawing on cutting edge research, unifies different perspectives into one global science: Canine Ergonomics.
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Detection dogs are highly accurate and flexible extensions of our senses. Despite improvements in machine intelligence and modern sensor technology, automated systems are still unable to match the operational effectiveness of trained canines in a number of activities such as explosive and narcotic detection (Fjellanger, Andersen, and McLean, 2002; Furton and Myers, 2001). There is, however, a persistent bias against using dogs in settings of consequence, like explosives detection, especially among engineers. Trevelyan (1997), who is an engineer, commented, “Dogs can smell explosive vapors, but the most sensitive detectors developed so far have orders of magnitude less sensitivity. Unfortunately, dogs are not consistent and treat their job as a game; they soon become bored.”
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Written by a neurobiologist and a psychologist, this volume presents a new theory of olfactory perception. Drawing on research in neuroscience, physiology, and ethology, Donald A. Wilson and Richard J. Stevenson address the fundamental question of how we navigate through a world of chemical encounters and provide a compelling alternative to the "reception-centric" view of olfaction. The major research challenge in olfaction is determining how the brain discriminates one smell from another. Here, the authors hold that olfaction is generally not a simple physiochemical process, but rather a plastic process that is strongly tied to memory. They find the traditional approach-which involves identifying how particular features of a chemical stimulus are represented in the olfactory system-to be at odds with historical data and with a growing body of neurobiological and psychological evidence that places primary emphasis on synthetic processing and experiential factors. Wilson and Stevenson propose that experience and cortical plasticity not only are important for traditional associative olfactory memory but also play a critical, defining role in odor perception and that current views are insufficient to account for current and past data. The book includes a broad comparative overview of the structure and function of olfactory systems, an exploration into the mechanisms of odor detection and olfactory perception, and a discussion of the implications of the authors' theory. Learning to Smell will serve as an important reference for workers within the field of chemical senses and those interested in sensory processing and perception. © 2006 by The Johns Hopkins University Press. All Rights Reserved.