Content uploaded by Pierre-Yves Morvan
Author content
All content in this area was uploaded by Pierre-Yves Morvan on Jul 10, 2015
Content may be subject to copyright.
A preview of the PDF is not available
Effect of a marine plant extract on skin melanogenesis
Abstract
Skin color comes from melanin pigments produced by melanocytes derived from the neural crest, in contact with the surrounding keranocytes via numerous dendrites and with nervous fibers. ▪ Hyperpigmentaon may be caused by sun damages, or numerous agents such as neurogenic peptides like endothelin‐1 and another peptide, Substance P, which has never been evaluated on melanogenesis. Objective ▪ Study the influence of two neuropeptides on melanogenesis in melanocytes, and evaluate its regulation by an extract of Pancratium maritimum (PME). The plant (also named Sea Lily) was cullvated in greenhouse. Results: Effect of PME on the synthesis of melanin by B16 melanocytes In the absence of NDP‐MSH (non‐smulated condiion), 0.33% PME significantly inhibits by 29% the melanin synthesis by the B16 melanocytes (fig 1A). In the presence of NDP‐MSH (100 nM), 0.165% and 0.33% PME significantly inhibits melanin synthesis by 32% and 65%, respecvely (fig 1B). Effects observed without any associated toxicity.
... These affect both neural and cellular stress pathways of melanin synthesis, ultimately providing an effective solution for dark spots. 16 Consistently following a comprehensive skincare regimen can significantly reduce age spots or dark spots. The current study revealed that HCPs recommend a complete regimen, including day and night creams along with a cleanser, to combat hyperpigmentation, dark spots, uneven skin tone, and dullness. ...
Background: Niacinamide has demonstrated its ability to treat dark spots in various studies. The present study evaluated the perspectives and experiences of healthcare professionals (HCPs) and patients with the niacinamide and sea daffodils-based bright healthy radiance product range for dark spots. Methods: This study was part of a survey series focusing on various dermatology products across India. The study involved over 1000 registered HCPs including dermatologists, pediatricians, and cosmetologists nationwide. A total of 203 HCPs participated in the survey on the niacinamide and sea daffodils-based product range. A well-structured questionnaire with 15 questions was used to collect the data and the responses were recorded by the HCPs. Results: Out of the total of 1086 individuals who participated in the survey, majority were females (774, 71.2%). Most HCPs reported that nearly 15-30% of their patients present with dark spots. A vast majority of individuals reported extreme satisfaction with the use of niacinamide and sea daffodils-based bright healthy radiance product range (493, 45.5%) and rated its effectiveness as excellent (511, 47.2%). HCPs reported that most patients showed major improvement in skin brightness (529, 48.7%), while the rest of them (418, 38.4%; 137, 12.6%) experienced good and satisfactory improvement respectively. Importantly, an even skin tone was achieved in most of the patients (492, 45.8%) after the application of the product range. Conclusions: A personalized approach of using niacinamide and sea daffodil-based products according to the patient's skin types and skin conditions is essential to remove blemishes and dark spots and improve skin brightness.
Background:
Key cellular players regulating human skin pigmentation include melanocytes in the epidermis that synthesize melanin, neighboring keratinocytes that receive and distribute melanin in the upper layers, and fibroblasts in the dermis that affect overlying melanocytes and keratinocytes. In addition, endocrine factors from the blood supply (endothelial cells) and inflammation-related factors play a role. Thus, new strategies for affecting pigmentation need to consider these multiple cell lines to adequately cover various causes and disease processes associated with hyperpigmentation.
Methods:
Pathophysiologic mechanisms and cellular pathways involved in melanogenesis were thoroughly reviewed with particular emphasis on the cellular interplay involved in the process. A complex system of interlinking and independent pathways was defined and described demonstrating differing pathways for altered pigmentary disorders - melasma associated with endothelial cell interactions; post inflammatory hyperpigmentation associated with keratinocyte inflammatory mediators (PGE2 in particular); and photodamage involving all 4 cell types. In vitro validation studies were then undertaken to define differing cell group gene expression profiles with selected peptides and other active agents. Melanocytic production of pigment was then tested with these agents to identify key potential players capable of limiting pigmentation.
Results:
Hexapeptide-12 and lactoferrin (melanocytes), Hexapeptide-11 (in keratinocytes), and phosphatidylserine (endothelial cells) were identified as major inhibitors of melanogenesis based on their gene expression profiles. This was confirmed by secondary melanin production tests performed on melanocytic lines. Additional active agents were also identified as inhibitors of melanocytic production of melanin, and together, these constituents formed the basis for a novel formulation for use in pigmentary disorders.
Conclusion:
A comprehensive scientific narrative of the various facets relating to pigmentation has been presented including differing pathways affecting varied cell lines that effect pigment production. Based on this concept, actives were tested using gene expression studies as well as in vitro melanogenic model testing in different cell lines. Using this novel multi-faceted approach, we have selected and validated a series of active agents to be used in a formulation targeting the complex problem of hyperpigmentation. J Drugs Dermatol. 2022;21(11):1206-1220. doi:10.36849/JDD.7013.
ResearchGate has not been able to resolve any references for this publication.