In this study, we investigated the β-lactamase-encoding genes responsible for β-lactam resistance phenotypes detected among fifty-six Gram-negative isolates (Gamma- and Alpha-proteobacteria) recovered from wastewater, urban streams and drinking water. The β-lactam resistance mechanisms detected in 36 isolates comprised the presence of class A (blaTEM-1 , blaSHV-1 , blaSHV-11 , blaGES-5 ), class B
... [Show full abstract] (ImiS, L1), class C (blaCMY-2 , blaCMY-34 , blaCMY-65 , blaCMY-89 , blaCMY-90 , blaACC-5 , blaACT-13 ), and class D β-lactamase-encoding genes (blaOXA-309 ), some variants described for the first time here. Notably, the results showed antimicrobial resistance genes related not only to commonly used antibiotics, but also to carbapenems, providing the first description of a GES-5-producing Enterobacteriaceae. The importance of ubiquitous bacteria thriving in aquatic environments as reservoirs or carriers of clinically-relevant resistance determinants was confirmed, and the need to monitor water habitats as potential sources for the emergence and/or spread of antibiotic resistance in the environment was highlighted. This article is protected by copyright. All rights reserved.