This study conducted a life cycle assessment (LCA) investigating energy, land occupation, greenhouse gas (GHG) emissions, fresh water consumption and stress-weighted water use from production of export lamb in the major production regions of New South Wales, Victoria and South Australia. The study used data from regional datasets and case study farms, and applied new methods for assessing water use using detailed farm water balances and water stress weighting. Land occupation was assessed with reference to the proportion of arable and non-arable land and allocation of liveweight (LW) and greasy wool was handled using a protein mass method. Fossil fuel energy demand ranged from 2.5 to 7.0 MJ/kg LW, fresh water consumption from 58.1 to 238.9 L/kg LW, stress-weighted water use from 2.9 to 137.8 L H2O-e/kg LW and crop land occupation from 0.2 to 2.0 m2/kg LW. Fossil fuel energy demand was dominated by on-farm energy demand, and differed between regions and datasets in response to production intensity and the use of purchased inputs such as fertiliser. Regional fresh water consumption was dominated by irrigation water use and losses from farm water supply, with smaller contributions from livestock drinking water. GHG emissions ranged from 6.1 to 7.3 kg CO2-e/kg LW and additional removals or emissions from land use (due to cultivation and fertilisation) and direct land-use change (due to deforestation over previous 20 years) were found to be modest, contributing between -1.6 and 0.3 kg CO2-e/kg LW for different scenarios assessing soil carbon flux. Excluding land use and direct land-use change, enteric CH4 contributed 83-89% of emissions, suggesting that emissions intensity can be reduced by focussing on flock production efficiency. Resource use and emissions were similar for export lamb production in the major production states of Australia, and GHG emissions were similar to other major global lamb producers. The results show impacts from lamb production on competitive resources to be low, as lamb production systems predominantly utilised non-arable land unsuited to alternative food production systems that rely on crop production, and water from regions with low water stress.