No full-text available
To read the full-text of this research,
you can request a copy directly from the author.
Correlation of Plasma homocysteine levels with BMI and Insulin Resistance, amongst Obese, Overweight and Non Obese Infertile Women
Abstract
Abstract- Background and Aims: The present study was
done to evaluate serum homocysteine level and its correlation
with metabolic (S. glucose level, S. insulin level and HOMA-IR.)
and anthropometric parameter (BMI) amongst over weight, obese
and non obese infertile women.
Methods: This cross sectional prospective study was conducted
in Department of Obstetrics and Gynaecology, CSM Medical
University, Lucknow, UP, India. After informed consent total 90
infertile women were enrolled, which included 29 overweight, 16
obese and 45 non-obese infertile women, who were taken as
controls. Blood samples were obtained in morning on 2nd day of
menstruation after an overnight fasting. During the same visit all
subjects underwent anthropometric measurement. Plasma
homocysteine concentration was measured by fluorescence
polarization immunoassay by using ABBOTT diagnostic kit
(USA). The estimation of insulin resistance by HOMA-IR score,
and BMI was calculated.
Results: Difference between the BMI, homocysteine levels,
insulin and HOMA-IR of three groups were statistically
significant (p<0.001). In obese group the mean homocysteine
level was (14.785.71), in overweight (9.845.16) and in non
obese (8.372.48). Result suggests association between
increasing homocysteine levels with increasing BMI. Insulin
resistant obese and overweight women had higher mean BMI
(29.912.32) than non insulin resistant (P<0.001). Serum
testosterone levels were significantly higher in obese
(65.0522.90) and over weight (50.1323.54) group as
compared to non obese (36.7519.38) group (P<0.001). A
positive correlation of total homocysteine levels was observed
with insulin (r=0.584), S. LH/FSH ratio (r=0.512) and HOMAIR(r=0.595).
Conclusions: We can conclude that a positive correlation was
observed between increasing homocysteine levels with BMI,
insulin resistance, testosterone levels and LH/FSH ratio.
... In addition, there was also a positive significant correlation between elevated serum insulin and IR in these patients [19]. Several evidences reported higher levels of sex steroid hormones and androgens in PCOS patients, which may influence the metabolism of Hcy and increase its plasma levels [20]. ...
between PCOS and genetic factors related to insulin resistance and type 2 diabetes mellitus [8]. In addition, a high risk of premature Coronary Artery Disease (CAD) has been described in patients with PCOS [9]. However, the exact etiology has not even been fully identified. To identify the pathogenesis for CAD in PCOS patients, several genetic studies have related to risk factors for CAD [10,11]. The risk factors associated with CAD are smoking , diabetes, family history, and increased homocyste-ine (Hcy) levels in PCOS [12]. Among the risk factors, it was described that the homocysteine level is extremely linked to insulin resistance in PCOS patients [13]. In Kazerooni and associates study, they proved to investigate the role of hyperandrogenemia, insulin resistance , and hyperhomocysteinemia as well as inherited and acquired thrombophilias in patients with PCOS who had Recurrent Pregnancy Loss (RPL). The writers establish that patients with PCOS and RPL had higher testos-terone, DHEAS, and homocysteine (Hcy) levels than patients without PCOS but with RPL. RPL in those patients with PCOS was also associated with hypofibrinolysis and decreased insulin sensitivity. Kazerooni and coworkers, also observed higher levels of Hcy in patients with RPL than in healthy people [14].
... 19 Several evidences reported higher levels of sex steroid hormones and androgens in PCOS patients, which may influence the metabolism of Hcy and increase its plasma levels. 20 Lastly, we conclude that elevated homocysteine in PCOS patients is a hazard factor for recurrent pregnancy loss. Folic acid supplementation in early pregnancy, in a pharmacological dose, might have a beneficial effect on pregnancy outcome. ...
Introduction: Polycystic ovary syndrome (PCOS) is one of the major causes of infertility
Worldwide and affects about 20% of infertile couples. Patients with PCOS have an
increased risk of first trimester spontaneous abortion ranging from 25% to 73%. Repeated
pregnancy loss (RPL), specified as two or more consecutive pregnancy losses before the
20th week of pregnancy is a frequent obstetric complication. In spite of this, 50% of cases
the pathophysiology remains unknown
Aim of the work: The purpose of this work was to prevent early recurrent pregnancy loss
in adult females with polycystic ovary syndrome and hyperhomocysteinemia by using folic
acid 5mg/daily.
Methods: The current study was carried out on 80 pregnant women in reproductive period,
their age ranged from 20 to 35 ages. They were assorted into two groups: group one
studying 40 women having a history of RPL with PCOS and group two including 40 adult
females having a history of RPL and non PCOS. The women in the two groups were treated
with 5mg/day folic acid/day for 20 weeks.
Results: Homocysteine level in patients with RPL and polycystic ovary was significantly
higher than in women without polycystic ovary syndrome. In addition, folic acid
administration at a daily dosage of 5 mg reduced significantly the rate of fetal loss.
Conclusion: We found that folic acid supplementation early in pregnancy, in a
pharmacological dose might have a beneficial effect on pregnancy outcome.
... the apparent association between Hc and cardiovascular diseases [1,4,9], and the possibility of regulating Hc synthesis through vitamin B 6 , vitamin B 12 and folic acid supply and regular physical exercise support taking preventive measures [16]. It is also important as elevated Hc levels correlate with insulin resistance [17,18], markers of oxidative stress [19,20], and inflammatory markers [21], which coexist with obesity and diabetes, and can be reduced through regular physical exercise. ...
Purpose
Homocysteine is a non-protein amino acid and elevated blood levels are often caused by inappropriate lifestyles, leading to atherosclerosis and cardiovascular diseases. The aim of this study was to determine the levels of homocysteine after a 9-month training program.
Methods
The group studied consisted of obese and overweight women, as well as women with normal body mass. During the training program, control examinations were carried out four times and the following parameters were analyzed: homocysteine blood levels, body mass, BMI, a single 10-minute exercise at a workload of 100W and the VO2max measured using the Astrand–Ryhming method.
Results
The highest homocysteine levels were found in obese women and the lowest in lean women. Higher levels were observed in older women (aged over 50 years) compared to younger women (aged below 50 years). The differences were clearly visible in obese women. Homocysteine levels decreased after the 9-month training program. A single exercise performed every three months during the training program resulted in an increase in homocysteine levels or no changes.
Conclusions
Higher homocysteine levels in the blood after a single exercise are likely caused by faster metabolism of this amino acid. Exercise of moderate intensity leads to a decrease in homocysteine levels in the blood, especially in obese women. Regular physical activity should therefore be recommended not only to prevent and treat obesity but, most importantly, atherosclerosis.
... Several studies on the relationship between Hcy and IR have been published, revealing conflicting results. Some investigations concluded that marked hyperhomocysteinemia could be a feature of IR (Meigs et al., 2001;Rekha et al., 2012;Mahalle et al., 2013). Moreover, some other studies have demonstrated an inverse relationship between these two well-known risk factors of atherosclerotic vascular disease, even in non-diabetic subjects ( Bar-On et al., 2000;Gallistl et al., 2000;Fonseca et al., 2003). ...
Diabetes mellitus is one of the most common chronic disorders in the world. Previous investigations have suggested that metformin, as a key treatment option in type 2 diabetes, may lead to hyperhomocysteinemia through decreasing the levels of vitamin B 12 and folic acid. In this clinical trial, 55 patients with type 2 diabetes who referred to two clinics of Shiraz University of Medical Sciences participated. At first, the participants followed diet regimen alone for 3 months and then in the next 3 months they were treated by metformin. Serum levels of vitamin B 12 , folic acid and homocysteine were checked before and after the diet alone and metformin consumption, then they were compared. After 3 months of diet regimen alone the levels of vitamin B 12 , folic acid and homocysteine did not show statistically (P≤0.05) significant differences. However, three months of treatment with metformin resulted in significantly higher levels of homocysteine (P=0.001) although serum levels of folic acid and vitamin B 12 did not change significantly again (P=0.1 and P=0.4, respectively). Our data provided no support for this hypothesis that metformin consumption increases homoysteine secondary to folate and /or B 12 deficiency.
... In this study, we found significant differences in Hcy levels and insulin resistance between the PCOS patients and the control group. The mean of Hcy and IR values in PCOS women was significantly higher than those in controls, which are in agreement with other previous studies [30][31][32]. When PCOS patients were divided into the insulin resistant and non-insulin resistant groups, higher prevalence of PCOS patients (76.66%) were insulin resistance. ...
Objectives: Polycystic ovary syndrome (PCOS) is a common disease among young women that causes hyperinsulinemia. Hyperhomocysteinemia is shown to be correlated positively with the hyperinsulinemia. This study aimed to evaluate serum homocysteine (Hcy) level and its correlation with other serum metabolic amongst over weight, obese and non-obese PCOS women.
Methods: Sixty women with PCOS and 20 healthy subjects were studied. Hormonal assays, lipid profile, Hcy and fasting insulin levels, insulin resistance (IR) indices (HOMA and QUICKI) determinations and ultrasound evaluation were performed.
Results: Mean of BMI was significantly higher in PCOS subjects as compared with the controls (29.95 ± 4.91 vs 26.09 ± 4.80 kg/m2; p = 0.005). The mean fasting insulin levels were also significantly higher (17.80 ± 8.02 vs 11.55 ± 6.81 μU/dl; p = 0.003) where we found no difference in fasting glucose concentrations between groups. IR indices in PCOS women were significantly higher than control group (p
... These findings might suggest the association of Polycystic Ovarian Syndrome (PCOS) with this state of hyper-androgenemia and high homocysteine plasma level. Similar results were obtained by Sachan et al .(2012). In contrast to this finding, George E et al found that DHEAS and testosterone level were not related to homocysteine level (George et al., 2006). ...
This study was conducted to assess homocysteine plasma level and insulin resistance profile (blood glucose level, plasma insulin level, HOMA-IR, body mass index) among different groups (non-obese, over-weight and obese) of women with anovulatory infertility. This cross-sectional study was conducted in Suez Canal University hospital in the period from December 2011 to August 2012. Total of 150 women with anovulatory infertility were included in this study, divided equally into three groups: non-obese, over-weight and obese. Blood samples were collected in second or third day of menstrual cycle for laboratory work-up. Hormonal profile and insulin resistance profile were determined for each patient. Plasma level of homocysteine was determined using the commercially available ELISA kit. Results showed that there were statistically significant differences between the three groups regarding homocysteine plasma level, body mass index and HOMA-IR with p-value < 0.001. There was a significant association between homocysteine plasma level and BMI. BMI and serum testosterone level were higher in obese and over-weight women in comparison to non-obese patients. Positive correlations were found between homocysteine plasma level with insulin level, HOMA-IR and LH/FSH ratio. In conclusion, homocysteine plasma level is positively correlated with BMI, insulin resistance, testosterone level and LH/ FSH ratio in over-weight and obese infertile women. This highlights an interaction between high homocysteine level, insulin resistance and hyperandrogenemia, mimicking polycystic ovarian syndrome that could be responsible for the infertile state in these patients.
Because of widespread chronic infections, especially among patients with heart and vascular inflammatory factors were measured three levels as vital functions Biomarkers for coronary heart disease, namely high-sensitivity C reactive protein hs-CRP, fibrinogen , homocysteine , and then finding the relationship between these factors and coronary heart disease CHD risk factors such as age , body mass index , blood pressure, blood sugar , and lipid profile .
The study was carried out in the Baquba teaching hospital from 1/ 9 / 2015 to 1/3 /2016, from patients who are lying in the recovery unit (CCU), consisting of :
- 35 patient with myocardial infarction (24 males, 11 females) .
- 35 infected with angina pectoris (19 males, 16 females) .
Both groups of patients were compared with a group of 20 healthy people (12 males, 8 females) , aged 40 – 70 years . Use 2 cm³ of blood to measure fibrinogen in blood by using coagulation homeostasis analysis device , Measurement homocysteine by technical Elisa , and measurement hs-CRP by use technical immune turbed metric preserved in temperature 20 C –homocysteine measurement and the other was placed in my Cobas Integra 400 Plus device other biochemistry indicators for measuring .
ResearchGate has not been able to resolve any references for this publication.